• 동의생리병리학회지
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• 제16권2호
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• pp.348-352
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• 2002

This study was carried out to evaluate cytotoxic effects of Atractylodes macrocephala Koidz. (A. macrocephala Koidz.) extract on NIH 3T3 fibloblast. SK-MEL-3 (HBT 69) and KB (ATCC No, OCL 17) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. 10.2 mg/ml Concentration of A. macrocephala Koidz. extracts in SK-MEL-3 showed that their susceptibility (sensitivity) to these compounds decreased in the following order ; adriamycin > H₂O > ethyl acetate > ethyl alcohol > chloroform > n-hexane in SK-MEL-3 cell lines ; 5-FU > H₂O > n-hexane > ethyl acetate > ethyl alcohol > chloroform in KB cell lines. In order to develop an antimicrobial agent, A. macrocephala Koidz. was extracted with solvents. The minimal inhibitory concentrations (MICs) of each solvent extract of A. macrocephala Koidz. against microogranisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of each solvent extract of A. macrocephala Koidz. The antimicrobial activity of the ethyl acetate soluble extract of A. macrocephala Koidz. had growth inhibition activity against S. mutans and P. putida (MICs. 500 ㎍/ml). These results suggest that the ethyl acetate soluble extract of A. macrocephala Koidz. possessed antitumorous and antimicrobial agents

• 대한수의학회지
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• 제41권4호
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• pp.511-521
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• 2001

Microorganisms were isolated and identified from bovine 296 quarters which showed positive reaction by California Mastitis Test (CMT) in 40 farms of Jeju from September 1999 to June 2000. The organisms associated with the mastitis of bovine were 11 different bacterial species in this study. Which of them, Staphylococcus aureus was the most predominant species as 152 (51.4%) isolates. Other identified species included 49 (16.5%) coliform, 47 (15.8%) Streptococcus dysgalactiae, 15 (5.1%) Bacillus spp., 8 (2.7%) Staphylococcus epidermidis, 6 (2.1%) Streptococcus agalactiae, 5 (1.7%) Enterococcus faecalis, 5 (1.7%) Corynebacterium spp., 3 (1.0%) Streptococcus uberis, 1 (0.3%) Pseudomonas aeruginosa and 1 (0.3%) Pasteurella haemolytica. Almost of all the islolated beacterial species showed high sensitivity against kanamycin (98.6%), cephalothin (98.0%), streptomycin (94.9%), gentamicin (94.6%), ampicillin (92.2%) and polymyxin B (90.2%). On the contrary, they showed resistance against penicillin (47.0%), tetracycline (37.2%), cefazolin (26.0%), bacitracin (22.6%) and erythromycin (19.9%). Eighty-one isolates were not resistant to any antibiotics and 215 drug resistant isolates showed 89 different drug resistance patterns from single to nine multiple antibiotics resistance patterns.

• International Journal of Oral Biology
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• 제40권1호
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• pp.41-50
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• 2015

The aim of this study was to identify bacteria isolated from the oral cavities and to determine their antimicrobial susceptibility against eight antibiotics. The bacterial strains were obtained from the Korean Collection for Oral Microbiology (KCOM). The bacteria were identified by comparing 16S rDNA sequences at the species level. The data showed that 77 bacterial strains were predominantly identified as streptococci (49.4%) and staphylococci (14.3%). Minimum inhibitory concentrations (MIC) were determined using a broth dilution assay to test the sensitivity of the bacterial strains. The MIC values of the oral bacterial strains against antibiotics were different. Streptococci were sensitive to clindamycin, cefuroxime axetil, and vancomycin, and they were resistant to tetracycline. Staphylococci also were sensitive to clindamycin, cefuroxime axetil, and vancomycin, and they were resistant to penicillin antibiotics. Gramnegative bacterial strains were sensitive to tetracycline and were resistant to clindamycin. These results suggest that the antimicrobial susceptibility test is necessary in deciding the prescription for antibiotics, to prevent the misuse or abuse of antibiotics.

• 한국환경보건학회지
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• 제19권1호
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• pp.71-76
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• 1993

Nasal carrige of Staphylococcus was studied in relation to its significance as a source of the Staphylococci that caused hospital-acquired infection. Due to the trend of the increasing r esistance of S. aureus to many antimicrobial agents, it is necessary to study the sensitivity to antibiotics of this infectious microorganisms. 50 persons from general hospital and 50 college students were the object of this study. The following results were obtained 3 0 Strains of S. aureus were isolated. The rate of S. aureus nasal carrying were 26% in college students and 34% in hospital personnel. S. aureus which showed resistance to penicillin were 90%, tetracyclin 43%, erythromycin 37% and oxacillin 17%. The number of penicillin resistance of S. aureus were 11 (84%) in college students and 16 (94%) in hospital personnel. The number of strains of penicillin resistant S. aureus which produced 13-1actamase were 9 (82%) in college student and 14 (88%) in hospital personnel. Methicillin-resistant S. aureus (MRSA) which showed resistance to erythromycin and penicillin G were 100%, tetracyline, cephalothin and clindamycin were over 40% respectively, gentamicin 20%, SAM 20% and chloramphenicol 0%.

• International Journal of Oral Biology
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• 제35권4호
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• pp.185-190
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• 2010

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most prevalent pathogens in hospitals. To investigate cross contamination by this bacterium in both dental and medical settings, the pathogens that cause acute pyogenic infection and one of the major microbes responsible for nosocomial infection were isolated from health care providers, nurses and patients. We used VITEK II to measure drug sensitivity, and we further performed biochemical testing, coagulase serotype testing and pulse-field gel electrophoresis (PFGE) for isolated MRSA colonies. The isolation rate of Staphylococcus aureus from nasal swabs was 75.0% from dental health care providers and 18.8% from the medical health care providers. A total of 10 MRSA strains were isolated from 40 health care providers and 2 patients and the prevalent coagulase serotype from patients and health care providers was VII. The antimicrobial drug resistance and partial PFGE types of the isolated MRSA strains showed a similar pattern. These results suggest that MRSA may be one of the principal causes of nosocomial infection in dental and medical hospitals.

• 한국식품과학회지
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• 제45권3호
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• pp.370-375
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• 2013

구강구취균 4종을 국내산 프로폴리스를 이용하여 프로폴리스의 농도, 열처리, pH의 변화를 통한 항균효과를 조사하였다. P. gingivalis KCTC 5352가 다소 프로폴리스에 저항적인 모습을 보였으나 그 외의 구강구취균에는 강한 항균활성을 나타내었다. 액체배지를 이용하여 프로폴리스의 농도를 조절하여 구강구치균의 민감도를 조사한 결과, 프로폴리스의 농도가 진할 수록 더 빠른 시간에 큰 활성을 보였으며 일반적으로 $0.22mg/{\mu}L$ 농도의 프로폴리스를 첨가하였을 경우 4-6시간 안에 대부분의 균이 사멸하였다. TEM을 통해 프로폴리스가 균의 세포에 어떤 영향을 미치는가 살펴보았으며 프로폴리스를 첨가한 균의 세포막이 얇아지면서 붕괴되고 안의 내부물질이 유출되면서 세포가 분해됨을 확인하였다. $0.56mg/{\mu}L$ 농도의 프로폴리스를 사용하여 열처리 후와 pH 조절 후의 프로폴리스의 항균활성을 시험한 결과 열에 안정하지 않고, pH가 높아질수록 활성이 약해지는 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1179-1188
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• 2023

Escherichia coli, particularly multidrug-resistant (MDR) strains, is a serious cause of healthcare-associated infections. Development of novel antimicrobial agents or restoration of drug efficiency is required to treat MDR bacteria, and the use of natural products to solve this problem is promising. We investigated the antimicrobial activity of dried green coffee (DGC) beans, coffee pulp (CP), and arabica leaf (AL) crude extracts against 28 isolated MDR E. coli strains and restoration of ampicillin (AMP) efficiency with a combination test. DGC, CP, and AL extracts were effective against all 28 strains, with a minimum inhibitory concentration (MIC) of 12.5-50 mg/ml and minimum bactericidal concentration of 25-100 mg/ml. The CP-AMP combination was more effective than CP or AMP alone, with a fractional inhibitory concentration index value of 0.01. In the combination, the MIC of CP was 0.2 mg/ml (compared to 25 mg/ml of CP alone) and that of AMP was 0.1 mg/ml (compared to 50 mg/ml of AMP alone), or a 125-fold and 500-fold reduction, respectively, against 13-drug resistant MDR E. coli strains. Time-kill kinetics showed that the bactericidal effect of the CP-AMP combination occurred within 3 h through disruption of membrane permeability and biofilm eradication, as verified by scanning electron microscopy. This is the first report indicating that CP-AMP combination therapy could be employed to treat MDR E. coli by repurposing AMP.

• 대한수의학회지
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• 제29권4호
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• pp.493-501
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• 1989

A total of 108 strains of C jejuni isolated from pigs and chickens were examined for the susceptibility to 10 antimicrobial agents and normal sera of cattle, sheep, guinea pigs and chickens. Minimal inhibitory concentration(MIC) ranges of antimicrobial agents to C jejuni isolates were $${\leq_-}1.56$$ to $${\geq_-}100{\mu}g/ml$$ for erythromycin, rifampin, streptomycin and tetracycline, 50 to $${\geq_-}100{\mu}g$$ for cephalothin, $${\leq_-}1.56$$ to $50{\mu}g$ for ampicillin, $${\leq_-}1.56$$ to $25{\mu}g$ for kanamycin and nalidixic acid, $${\leq_-}1.56$$ to $12.5{\mu}g$ for chloramphenicol and gentamicin. Resistance rates of C jejuni were showed to in order of rifampin(84.7%), tetracycline(56.2 %), erythromycin(17.1%) and ampicillin(3.8%), all of the strains were sensitive to chloramphenicol, gentamicin and kanamycin, and the incidence rates of resistant C jejuni were highly frequent in pig isolates than chicken isolates. The drug resistance patterns of 87 chicken isolates C jejuni to 9 antimicrobial drugs were showed 12 patterns, and Sm Ra Tc(24.1%), Sm Ra(21.8%) and Ra Tc(14.9%) were relatively common, and also 21 pig isolates were showed 6 patterns and Em Sm Ra Tc(57.1%) were most frequent. The majority of the isolates showed multiple drug resistance. Bactericidal activity of 10% normal sera from healthy animals were examined for 60min at $37^{\circ}C$. C jejuni were decreased from 0.4 to 1.0 ${\log}_{10}$(p<0.01), and serum susceptibility were high in order of guinea pig, sheep, chicken and cattle sera. Serum sensitivity of C jejuni Ch-39 strain in increased serum concentation up to 10, 20, 40 and 80% were highly significant. In the normal animal serum, the number of Ch-39 strain were decreased from $1.8{\times}10^4/ml$ to $2.7{\times}10^3/ml$ after 60 min incubation(p<0.01), but the numbers were decreased to $6.6{\times}10^3/ml$ in the heat inactivated normal serum for 30 min at $56^{\circ}C$. Bactericidal activity was restored in the heat inactivated normal serum after the serum of complement source was added.

• 한국어병학회지
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• 제22권1호
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• pp.53-65
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• 2009

Tenacibaculum maritimum (formerly Flexibacter maritimus)은 tenacibaculosis라 불리며, 해산어에서 궤양 및 괴사성 증상을 나타내는 질병원인체 이다. Tenacibaculosis는 다양한 양식 어종에서 있어서 경제적 측면에서도 중요한 질병 중 하나이며, 피부, 입, 지느러미, 꼬리 등과 같은 어체 표면에 심각한 궤양과 괴사로 인한 손상을 유발시 킨다. 본 연구에서는 tenacibaculosis의 증상을 나타내는 넙치 병어로부터 A-7 균주를 분리하였고, 형태학적 특성, 생화학적 성상 및 유전자 분석 결과를 바탕으로 T. maritimum으로 동정하였으며, 침지법을 통하여 넙치에 인위적으로 감염되는 것을 확인하였다. Tenacibaculosis를 제어하기 위해 약욕에 의한 항생제 투여가 많이 이루어지며, 그 중 oxytetracycline이 주로 사용되고있다. 그러나, 대부분의 양식어민과 소비자들은항생제 잔류와 환경오염 등의 문제를 우려하고있으며, 비싼 항생제의 사용으로 인하여 양식어의 생산 비용도 높아지고 있다. 이러한 문제점들을 극복하기 위하여 본 연구에서는 천연 자생식물 추출물을 항생제 대체제로 사용하기 위한 연구를 수행하였다. 총 35 종류의 천연식물의 항균 활성을 검색하였고, 많은 천연식물 추출물이 T. maritimum에 대하여 높은 항균 효과를 나타낸다는 것을 밝혔다.

• 환경위생공학
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• 제23권2호
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• pp.71-79
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• 2008

We examined 165 unchlorinated natural drinking water samples for the presence of E. coli group resistant to antimicrobial agent and chlorine in nothern Gyeongbuk area in 2007. Among 165 water samples, 21 samples(12.7%) were positive to total coliforms and Six genus, 16 strains of E. coli groups isolated from 16 samples showed resistance against more than one antimicrobial agent such as Ampicillin, Tetracycline and Chloroamphenicol. Among 16 strains, 14 strains resistant to Ampicillin, 9 strains resistant to Tetracycline and one strain resistant to Chloroampenicol. but all 16 strains did not contain any integron gene cassettes, which contribute to the spread of antimicrobial resistance alleles by lateral gene transfer of gene cassettes in a variety of enteric bacteria. The minimal inhibitory concentration(MIC) of 14 strains which showed resistant to Ampicillin was between $12{\mu}g/m{\ell}$ and $32{\mu}g/m{\ell}$, Nine strains resistant to Tetracycline showed between $32{\mu}g/m{\ell}$ and $128{\mu}g/m{\ell}$ and one strain resistant to Chloroampenicol showed $128{\mu}g/m{\ell}$. The chlorine sensitivity of 16 strains isolated from unchlorinated natural water sample did not show any difference among strains by the concentration of initial free chlorine and elapsed time after chlorine treatment. All 16 strains were killed after 1hr. exposure at $0.2mg/m{\ell}$ of free chlorine per liter or 30minutes exposure at $0.4mg/m{\ell}$ of free chlorine per liter.