• YAKHAK HOEJI
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• v.16 no.4
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• pp.162-175
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• 1972

Fifty six compounds of 4-methylthiosemicarbazone and thiorcarbohydrazone derivatives were prepared and subjected to biological tests. The following five compounds, 2-hydroxybenzaldehyde monothiocarbohydrazone (2),4-methylbenzaldehyde monothiocarbohydrazone (8), 1-(2-hydroxybenzaldehyde)-5(4-hydroxy-3-methoxybenzaldehyde) dithiocarbohydrazone (45), 1-(2-hydroxybenzaldehyde)-5-furfural dithiocarbohydrazone (46) and 1-benzaldehyde-5-cinnamaldehyde dithiocarbohydrazone (49) exhibited marked antimicrobial activity against E. coli, St. aureus and P. chrysogenum. In addition to these compounds, 3-methoxybenzaldehyde monothiocarbohydrazone (12) and 4-methylbenzaldehyde dithiocarbohydrazone (29) showed marked inhibition of HeLa cell growth at the concentration of 10 ${\nu}$g/ml. It was generally observed that most compounds demonstrated significant antifungal activity against P. chrysongenum but only one compound, 3-hydroxy-4-methoxybenzaldehyde dithiocarbohydrazone (39), exerted antituberculosis activity against M. tuberculosis H$_{37}$ RV at the concentration of 10 ${\nu}$g/ml.

• YAKHAK HOEJI
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• v.50 no.1
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• pp.15-20
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• 2006

Puerarin and daidzin have been isolated from Puerariae thunbergiana Benth. Structures were determined by spectroscopic methods. Compounds showed weak antimicrobial activity against S. mutans, S. epidermidis, S. aureus, and C. albicans (MIC, $800{\mu}g/ml$). However, these compounds were not antioxidative. Puerariae thunbergiana Benth. extracts against microorganisms were evaluated in terms of the minimum inhibitory concentrations (MIC). In general, C. albicans was stronger antimicrobial activity than the other microorganisms. The antioxidative activity of was observed in the etyl acetate extract ($IC_{50},\;119.87{\pm}0.16\;{\mu}g/ml$). The DPPH radical scavenging effect ($IC_{50},\;1,673.3{\pm}0.54\;{\mu}M$) of the puerarin was comparable with that of synthetic antioxidant, BHA $(IC_{50},\;88.39{\pm}1.1){\times}10^{3}{\mu}M$. These results suggest that puerarin and daidzin have a potential antimicrobial activity.

• Korean Journal of Food Science and Technology
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• v.22 no.6
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• pp.716-719
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• 1990

The essential oils were isolated by steam distillation from 13 spices used for curry. Antimicrobial activity of essential oils for two strains of Gram(+) bacteria, Gram(-) bacteria, lactic acid bacteria, yeast and mold were investigated by agar diffusion method. 5 spice essential oils(clove, cumin, nutmeg, oregano, rosemary) having high antimicrobial activity were selected and their minimal inhibitory concentration(MIC) were measured. Very low concentration ($0.2{\sim}9\;mg/ml$) of 5 spice essential oils were sufficient to prevent microbial growth. The data show that Gram(+) bacteria were more sensitive to the antimicrobial compounds in spices than Gram(-). But though Gram(+) bacteria, lactic acid bacteria were less sensitive to the compounds than Gram(-).

• Natural Product Sciences
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• v.25 no.1
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• pp.38-43
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• 2019

Microbial wound infection prolonged the hospitalization and increase the cost for wound management. Silver is commonly used as antimicrobial wound dressing. However, it causes several adverse side effects. Hence, this study was aimed to evaluate the antimicrobial efficiency of Swietenia macrophylla seed extract on clinical wound pathogens. Besides, the bioactive constituents of the seed extract were also determined. S. macrophylla seeds were extracted with methanol by maceration method. The seed extract inhibited 5 test bacteria and 1 yeast on disc diffusion assay. The antibacterial activity was broad spectrum, as the extract inhibited both Gram positive and Gram negative bacteria. On kill curve analysis, the antibacterial activity of the seed extract was concentration-dependent, the increase of extract concentration resulted in more reduction of bacterial growth. The extract also caused 99.9% growth reduction of Bacillus subtilis relative to control. A total of 21 compounds were detected in gas chromatography- mass spectrometry analysis. The predominant compounds present in the extract were oleic acid (18.56%) and linoleic acid (17.72%). In conclusion, the methanolic extract of S. macrophylla seeds exhibited significant antimicrobial activity on clinical wound pathogens. Further investigations should be conducted to purify other bioactive compounds from the seeds of S. macrophylla.

• Journal of Microbiology and Biotechnology
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• v.25 no.5
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• pp.629-636
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• 2015

Chemical investigation of the actinobacterial isolate Pseudonocardia endophytica VUK-10 has led to the segregation of two known bioactive compounds, namely 4-(2-acetamidoethyl) phenyl acetate and 4-((1, 4-dioxooctahydropyrrolo [1, 2-a] pyrazin-3-yl) methyl) phenyl acetate. The strain was isolated from a sediment sample of the Nizampatnam mangrove ecosystem, south coastal Andhra Pradesh, India. The chemical structure of the active compounds was established on the basis of spectroscopic analysis, including ¹H NMR and ¹³C NMR spectroscopies, FTIR, and EIMS. The antimicrobial and cytotoxic activities of the bioactive compounds produced by the strain were tested against opportunistic and pathogenic bacteria and fungi and on MDA-MB-231, OAW, HeLa, and MCF-7 cell lines. The compounds exhibited antimicrobial activities against gram-positive and gram-negative bacteria and fungi and also showed potent cytotoxic activity against MDA-MB-231, OAW, HeLa, and MCF-7 cell lines. This is the first example for this class of bioactive compounds isolated from Pseudonocardia of mangrove origin.

• Bulletin of the Korean Chemical Society
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• v.32 no.11
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• pp.3887-3892
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• 2011

An efficient synthesis of 1,5-Benzothiazepines via Michael addition of corresponding (E)-1-(2-hydroxyphenyl)-3-(4-(phenylthio)phenyl)prop-2-en-1-one is described under ultrasound irradiation. A series of novel 2-((E)-2,3-dihydro-2-(4-(phenylthio)phenyl)benzo[b][1,4]thiazepin-4-yl)phenol derivatives was confirmed on the basis of $^1H$ NMR, Mass, IR spectral data and Elemental analysis. The synthesized compounds were evaluated for their antimicrobial activities. Most of the compounds were found to be comparable potent than the reference standard drugs. Utilization of ultrasound irradiation, simple reaction conditions, isolation, and purification makes this manipulation very interesting from an economic and environmental perspective.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.17 no.1
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• pp.126-130
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• 2004

Objectives; Bioassay-directed fractionation of the dried roots of Salvia miltiorrhiza led to the isolation of abietane tanshinones, crptotanshinone and dihydrotanshinone. Methods: Their structures were elucidated using 1H-and 13C-NMR, UV, IR and mass spectral analysis. Result and Conclusions : These compounds exhibited a moderate antimicrobial activities against Staphylococcus epidemidis, Staphylococcus aureus, Staphylococcus pyogene. Actinobacillus actinomycetemcomitans, Escherichia coil, Enterbacter cloacae, Pseudomonas aeruginosa, Citrobacter freundii, and Samonella typhimurium.

• Natural Product Sciences
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• v.15 no.2
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• pp.76-82
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• 2009

Phytochemical investigation of the EtOAc and n-BuOH fractions obtained from the crude methanol extract of the seeds of Butyrospermum parkii led to the isolation of seven compounds including 1${\beta}$,2${\alpha}$,3${\alpha}$-trihydroxyurs-12-en-28-oic acid (1${\beta}$-hydroxyeuscaphic acid) which full NMR assignment is herein reported for the first time. The above extract, fractions and some of the isolated compounds were screened for antimicrobial and antioxidant activities; 1${\beta}$-hydroxyeuscaphic acid was the more potent.

• Archives of Pharmacal Research
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• v.23 no.4
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• pp.310-314
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• 2000

The synthesis and spectral analysis of some new 1,3-dihydro-3-hydroxy-3-[2-hydroxyimino-2-(substituted phenyl)ethyll-2H-indol-2-ones (21-32) and spiro[3H-indol-3,5'-(4'H)-isoxazol]-2(1 H)-ones (33-44) are described. Sixteen of the synthesized compounds were screened in vitro for their growth inhibitory activity against thirteen species of microorganisms, viz, S. aureus, S. epidermidis, S. faecalis, B. subtilis, B. cereus, E. aerogens, E. coli, P. aeruginosa, P. vulgaris, A. baumonia, A. faecalis, C. albicans and S. cervicae. Most of the compounds exhibited significant antimicrobial activity especially the oximes 28 and 29.

• Journal of the Korean Chemical Society
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• v.68 no.4
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• pp.199-204
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• 2024

Chamaecyparis obtusa (Siebold & Zucc.) Endl. is an evergreen tree of the family Cupressaceae well known for its unique scents. The seed extract of this cypress tree was phytochemically investigated to isolate a novel abietane-diterpene compound (1) along with fifteen known terpenoids (2-16). All of the isolated compounds were subjected to the screening of antimicrobial activities against Cutibacterium acnes and Staphylococcus epidermidis including erythromycin resistant strains. Among the isolates, 1α-hydroxy-hinokione (1), hinokione (3), 1,2-dehydrohinokione (4) and ferruginol (9) showed significant antibacterial activities against both acne-causing strains. This study demonstrated that abietane-type diterpenoids are the main antibacterial components in C. obtusa seed extract, and some isolated compounds can be further developed as potential acne-treatment agents.