• 한국수의병리학회지
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• 제7권1호
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• pp.11-15
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• 2003

Antigen retrieval (AR) techniques were widely used to recover the antigenicity from the fixed tissues, which were guided by the philosophy of rendering immunohistochemistry (IHC) applicable to routine formalin-fixed, paraffin-embedded tissues for wide application of IHC in research and clinical filed for morphological observation like as anatomy, histology and pathology. Protease antigen recovery (PAR) is an AR technique, which is obtained the antigen retrieve by using enzyme digestion, and commonly used in IHC field. However, during the IHC for the detection of ovine herpesvirus 2 (OvHV-2) antigen, we noted lymphocyte-like cells-specific staining in the infiltrated cells into various organs like as liver and kidney, which was also shown in the IHC tissues with isotype control. However, those signals were not observed in the tissues conducted with in situ hybridization. Therefore, we analyzed the specificity of the IHC detection results. We found that PAR may induce false-positive result during IHC in lymphocyte-like cells, which were infiltrated mainly around vessels and in interstitial tissues. Through the Phenotyping, we realized that those false-positive cells were B-cell-related cells. These results suggest that PAR, a AR using protease, may induce non-specific false-positive reactions during IHC.

• 대한세포병리학회지
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• 제9권1호
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• pp.15-20
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• 1998

Mutant form of the p53 gene product is abnormally accumulated in the nuclei of the tumor cells due to prolonged half life, and readily detected by immunohistochemical methods. To determine the positivity rate of p53 in body cavity fluid according the primary site and histological types of tumors and the utility of p53 immunostaining as an adjunct in the diagnosis of malignancy, we reviewed 69 effusions, including pleural effusion, ascitic fluid, and pericardial fluid, that were diagnosed as overt malignancy and 21 effusions of suspicious malignancy, immunohistochemistry was performed on paraffin-embedded cell blocks using a monoclonal antibody to p53 supressor gene product(Clone DO7) and a standard avidin-biotin complex technique with a citrate buffer antigen retrieval solution. The results were as follows; of the 46 pleural effusions with overt malignancy, 22 were immunopositive for p53 protein; of the 21 ascitic fluids with overt malignancy, 5 were positive for p53. Positivity rates according to the primary sites of tumors were 18 of 34(52.9%), 8 of 21(38.1%), 1 of 9(11.1%) cases of the tumors of the lung, GI tract, and ovary, respectively. According to the histologic types of lung cancer, 11 cases(61.6%) were positive out of 18 adenocarcinomas, 2 of 5 large cell undifferentiated carcinomas, and 1 of 2 small cell undifferentiated carcinomas. Of 21 cases of suspicious malignancy, 6 were positive for p53 and all of them(6/6) were confirmed as adenocarcinoma of the lung or GI tract. These findings indicate that p53 immunostaining using paraffin embedded cell block is useful diagnostic and prognostic marker in body fluid cytology although negative immunostaining does not exclude malignancy.

• 한국임상수의학회지
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• 제22권4호
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• pp.309-313
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• 2005

본 연구는 심장사상충 감염견에 있어서 flexible alligator forceps(FAF)을 이용한 외과적 치료법의 효능을 평가하기 위해 실시되었다. FAF를 이용한 외과적 치료를 위해 평균 연령 $5.3\pm3.0\;(mean{\pm}S.D.)$ 년, 평균 체중 $9.3\pm6.5(mean{\pm}S.D.)kg$인 20마리의 심장사상충 감염견을 실험에 사용하였다 그 중 수컷 10마리, 암컷 10마리였다. 성충 제거를 위한 외과적 치료를 isoflurane호흡 마취 하에 FAF완 투시기를 이용하여 실시하였다 FAF를 이용한 외과적 치료방법의 평가를 위해 제거율과 수술시간을 측정하였고 제거 부위를 확인하였다 제거되지 알고 남아 있을 수 있는 성충 확인을 위해 항원 ELISA kit검사와 부검을 실시하였다. 제거율은 심장사상충이 완전히 제거된 15마리를 포함 전체 심장사상충의 $91.4\%$ 였고 수술에 걸린 평균 시간은 $30.0\pm7.6(mean{\pm}S.E.)$이었다. 또한 심장사상충은 우심실, 주폐동맥, 우폐동맥, 그리고 좌폐동맥에서 제거되었다 결론적으로, FAF를 이용한 외과적 치료법은 높은 제거율, 짧은 수술 시간, 그리고 다양한 제거부위와 같은 장점을 보이는 효과적이 방법이며 심장사상충 감염견에서 적용될 수 있는 좋은 치료법으로 사료된다.