• 생명과학회지
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• 제17권7호통권87호
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• pp.944-947
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• 2007

Bacillus LPO3 (계면활성제와 같이 유화물질을 생산하는)을 hydrocarbon을 감소시키는 bio-control agent로 사용했다. 토양 (시중구입)은 gasoline을 섞어 오염시킨 토양을 사용하였다. 13일 동안 bacteria의 성장, hydrocarbon의 감소, 그리고, Bacillus LP03 의 작물 성장에 미친 요인(식물의 싹과 뿌리의 길이를 포함한)을 관찰하였다. 우리는 이 bacteria의hydrocarbon을 감소 시키는 물질로서 이 균이 생산하는 계면활성제일 것이라는 가능성을 이미 확인 하였으며 이는 식물의 싹과 뿌리의 생장을 촉진하였고, 회색 곰팡이에 대하여항 진균 활성을 보였다. 본 연구에서는 bacteria를 넣지 않은 기름으로 오염된 토양 실험 군에서는 식물이 자라나지 않았을 뿐 아니라 살아남지도 못했다. 실험 결과로 볼 때, gasoline의 hydrocarbon이 시간이 지남에 따라 감소되었고, RNA blotting실험에서는 오염된 토양에서 균의 증식과 더불어 계면활성제관련 유전자 산물의 증가를 확인하였다 결과적으로, 이 bacteria (계면활성제를 생산하는)는 회색 곰팡이에 감염된 토양이나 기름으로 오염된 토양에서 작물이 자랄 수 있게 하기 위한 bio-control agent로서 유효한 균으로 생각된다.

• 한국건설순환자원학회논문집
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• 제10권1호
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• pp.117-123
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• 2022

현대인은 대부분의 시간을 실내에서 보내고 있기 때문에 실내 공기질에 대한 관심이 높아지는 추세이다. 실내 공기의 주요 오염원으로는 인간의 활동, 건축자재, 생활용품, 외부로부터 오염된 대기의 유입 등을 들 수 있다. 실내 공기질 관리법에서는 실내공기 오염물질로 미세먼지, 총 부유세균, 곰팡이, 이산화탄소 등 17종을 규정하고 있다. 현대인의 실내 환경은 세균과 곰팡이균의 생육환경이 흡사하기 때문에 일상생활에서 세균과 곰팡이에 항상 노출된다. 특히 많은 사람이 모이는 다중이용시설의 경우에는 세균감염의 위험이 더 크다고 할 수 있다. 한편 솔잎은 예전부터 항균효과가 뛰어난 것으로 알려져 있으며, 한약재, 식용 등 쓰임새가 다양하다. 그 외에 의약, 식품 및 염색제 관련 산업에서는 적극적으로 활용하고 있으며, 최근에는 건축자재로 이용하기 위한 시도도 이루어지고 있는 실정이다. 솔잎의 항균효과에 관한 연구는 상당히 많이 이루어지고 있으며, 다양한 항균 성분을 가지고 있다. 따라서 본 연구는 솔잎 추출물의 항세균, 항곰팡이 효과를 확인하였으며, 이를 토대로 솔잎 추출물을 혼입한 시멘트 경화체를 제작해 솔잎 추출물의 혼입 비율별 세균과 곰팡이에 대한 항균 활성 효과를 연구하였다. 솔잎 추출물은 황색포도상구균과 대장균에 대해 항세균 효과를 나타났으며, 검정 곰팡이에 대해 생육환경을 저해하는 것이 나타났다. 솔잎 추출물을 혼입한 시멘트 경화체는 황색포도상구균과 대장균에 대해서 항균 활성 효과를 나타냈다. 그러나 검정 곰팡이에 대해서 항균 활성 효과를 나타내지 않았다. 결과적으로, 솔잎추출물을 혼입한 시멘트 경화체는 병원균 억제에는 효과적이지만 곰팡이에 대해서는 효과가 없는 것으로 판단되며, 솔잎추출물을 항균재료 사용하기 위해서는 곰팡이의 생육을 저해하는 요인에 대한 추가적인 연구가 필요할 것으로 판단된다.

• Natural Product Sciences
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• 제23권2호
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• pp.125-131
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• 2017

Clitoria ternatea or Commonly known blue pea, is a perennial climber crop native to Asian countries. The current study was aimed to evaluate the antimicrobial activity C. ternatea extract on food borne microorganisms and its antifungal effect on Penicillium expansum. The extract showed significant antimicrobial activity against 3 Gram positive bacteria, 2 Gram negative bacteria and 1 filamentous fungus on disc diffusion assay. The extract also showed good biocidal effect on all Gram positive bacteria tested and P. expansum. However, the kill curve analysis revealed that the fungicidal activity of the extract against P. expansum conidia was depend on the concentration of the extract and the time of exposure of the conidia to the extract. The scanning electron micrograph of the extract treated P. expansum culture showed alterations in the morphology of fungal hyphae. The germination of P. expansum conidia was completely inhibited and conidial development was totally suppressed by the extract, suggesting the possible mode of action of anthocyanin. Besides, the extract also exhibited 5.0-log suppression of microbial growth relative to control in the rice model. The results indicate the potential use of the C. ternatea anthocyanin as food biopreservative.

• Mycobiology
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• 제40권4호
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• pp.244-251
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• 2012

In vitro and greenhouse screening of seven rhizobacterial isolates, AB05, AB10, AB11, AB12, AB14, AB15 and AB17, was conducted to investigate the plant growth promoting activities and inhibition against anthracnose caused by Colletotrichum acutatum in pepper. According to identification based on 16S rDNA sequencing, the majority of the isolates are members of Bacillus and a single isolate belongs to the genus Paenibacillus. All seven bacterial isolates were capable of inhibiting C. acutatum to various degrees. The results primarily showed that antibiotic substances produced by the selected bacteria were effective and resulted in strong antifungal activity against the fungi. However, isolate AB15 was the most effective bacterial strain, with the potential to suppress more than 50% mycelial growth of C. acutatum in vitro. Moreover, antibiotics from Paenibacillus polymyxa (AB15) and volatile compounds from Bacillus subtilis (AB14) exerted efficient antagonistic activity against the pathogens in a dual culture assay. In vivo suppression activity of selected bacteria was also analyzed in a greenhouse with the reference to their prominent in vitro antagonism efficacy. Induced systemic resistance in pepper against C. acutatum was also observed under greenhouse conditions. Where, isolate AB15 was found to be the most effective bacterial strain at suppressing pepper anthracnose under greenhouse conditions. Moreover, four isolates, AB10, AB12, AB15, and AB17, were identified as the most effective growth promoting bacteria under greenhouse conditions, with AB17 inducing the greatest enhancement of pepper growth.

• Mycobiology
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• 제29권4호
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• pp.218-223
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• 2001

A rapid radicle assay for prescreening antagonistic bacteria to Phytophthora capsic4 causal agent of Phytophthora blight of pepper was developed. Sixty-four bacterial strains with in vitro antifungal activity selected out of 1,400 strains isolated from soils of Ansung, Chunan, Koyang, and Paju, Korea in 1998 were used for development of the bioassay. Uniformly germinated pepper seeds dipped in bacterial cells for 3 hours were placed near the edges of growing mycelia of P. capsici on water agar containing 0.02% glucose. Five-week-old pepper plants(cv. Nockwang) were inoculated to compare with results of the radicle assay developed in this study. For plant inoculation, pepper seeds were sown in potting mixtures incorporated with the bacterial strains, then transplanted into steam-sterilized soils 3 weeks later. Plants were hole-inoculated with zoospores of P. capsici 2 weeks after transplanting. Disease incidence and severity were determined in radicle and plant assessments, respectively. In radicle assay, six strains, GK-B15, GK-B25, OA-B26, OA-B36, PK-B09, and VK-B14 consistently showed the significant(P=0.05) disease reduction against radicle infection by the fungus, four of which also did in plant assessments. Strains OA-B36 and GK-B15 consistently reduced the fungal infection in both the radicle assay and the plant assessment. Therefore, prescreening strains using the radicle assay developed in this study followed by plant assay could reduce time and labor, and improved the possibility of selecting antagonistic bacteria for control of Phytophthora blight of peppers.

• Korean Chemical Engineering Research
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• 제56권2호
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• pp.245-251
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• 2018

본 연구에서는 커피(Coffea arabica)의 에탄올 추출물을 이용하여 기능성 화장품소재로서의 가능성을 검토하였다. 미백활성으로는 tyrosinase 저해 활성과 멜라닌 함량을 측정하였으며, 항산화 활성으로는 SOD-like activity를 측정하였고, 그 결과 $500{\mu}g/mL$의 농도에서 $94.8{\pm}6.2%$의 활성을 나타내었다. CAE는 3개의 그람양성균과 6개의 그람음성균을 대상으로 항균활성을 조사하였으며, 그 결과 그람음성균에 대해서는 폭넓은 활성을 나타내었지만, 그람양성균에 대한 활성은 나타내지 않았다. 하지만 항생제내성균주인 MRSA CCARM3561에는 강한 활성을 나타내었다. 반면에 Candida albicanse에 대한 항곰팡이 활성은 나타내지 않았다. Tyrosinase 저해활성과 DOPA 산화능은 대조군인 arbutin 보다는 낮게 나타내었고, B16-F10 melanoma cell에 대한 멜라닌 생합성 저해능은 농도 의존적으로 감소하는 경향을 보였으며, $100{\mu}g/mL$의 농도에서 89.2%를 나타내었다. 이러한 결과들은 커피 추출물이 천연물, 무독성 항균제 또한 천연 화장품 소재로써의 가치가 있음을 나타내는 것이다.

• Advances in nano research
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• 제5권2호
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• pp.127-140
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• 2017

Metal nanoparticles have been intensively studied within the past decade. Nano-sized materials have been an important subject in basic and applied sciences. Zinc oxide nanoparticles have received considerable attention due to their unique antibacterial, antifungal, and UV filtering properties, high catalytic and photochemical activity. In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated. In the emerging issue of increased multi-resistant properties in water borne pathogens, zinc oxide (ZnO) nanoparticle are being used increasingly as antimicrobial agents. Thus, the minimum bactericidal concentration (MBC) and minimum fungal concentration of ZnO nanoparticles towards pathogens microbe were examined in this study. The results obtained suggested that ZnO nanoparticles exhibit a good anti fungal activity than bactericidal effect towards all pathogens tested in in-vitro disc diffusion method (170 ppm, 100 ppm and 30 ppm). ZnO nanoparticles can be a potential antimicrobial agent due to its low cost of production and high effectiveness in antimicrobial properties, which may find wide applications in various industries to address safety issues. Stable ZnO nanoparticles were prepared and their shape and size distribution characterized by Dynamic light scattering (35.7 nm) and transmission electron microscopic TEM study for morphology identification (20 nm), UV-visible spectroscopy (230 nm), X-ray diffraction (FWHM of more intense peak corresponding to 101 planes located at $36.33^{\circ}$ using Scherrer's formula), FT-IR (Amines, Alcohols, Carbonyl and Nitrate ions), Zeta potential (-28.8). The antimicrobial activity of ZnO nanoparticles was investigated against Bacteria and Fungi present in drinking water PVC pipelines biofilm. In these tests, Muller Hinton agar plates were used and ZnO nanoparticles of various concentrations were supplemented in solid medium.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1029-1039
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• 2024

This study explores beneficial bacteria isolated from the roots and rhizosphere soil of Khao Rai Leum Pua Phetchabun rice plants. A total of 315 bacterial isolates (KK001 to KK315) were obtained. Plant growth-promoting traits (phosphate solubilization and indole-3-acetic acid (IAA) production), and antimicrobial activity against three rice pathogens (Curvularia lunata NUF001, Bipolaris oryzae 2464, and Xanthomonas oryzae pv. oryzae) were assessed. KK074 was the most prolific in IAA production, generating 362.6 ± 28.0 ㎍/ml, and KK007 excelled in tricalcium phosphate solubilization, achieving 714.2 ± 12.1 ㎍/ml. In antimicrobial assays using the dual culture method, KK024 and KK281 exhibited strong inhibitory activity against C. lunata, and KK269 was particularly effective against B. oryzae. In the evaluation of antimicrobial metabolite production, KK281 and KK288 exhibited strong antifungal activities in cell-free supernatants. Given the superior performance of KK281, taxonomically identified as Bacillus sp. KK281, it was investigated further. Lipopeptide extracts from KK281 had significant antimicrobial activity against C. lunata and a minimum inhibitory concentration (MIC) of 3.1 mg/ml against X. oryzae pv. oryzae. LC-ESI-MS/MS analysis revealed the presence of surfactin in the lipopeptide extract. The crude extract was non-cytotoxic to the L-929 cell line at tested concentrations. In conclusion, the in vitro plant growth-promoting and disease-controlling attributes of Bacillus sp. KK281 make it a strong candidate for field evaluation to boost plant growth and manage disease in upland rice.

• 생약학회지
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• 제19권2호
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• pp.120-126
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• 1988

HPLC analysis of three Prunus species, Armeniacae Semen. Persicae Semen, and Mume Semen, showed that 24. 06 mg/g, 5. 79 mg/g and 3/ 10 mg/g of amygdalin in the MeOH extract and 3. 59 mg/g, 5. 41 mg/g and 13. 48 mg/g of benzaldehyde in the MeOH extract hydrolyzed with ${\beta}-glucosidase$ were contained respectively. The MeOH extract of Mume Semen showed strong antibacterial activities against two species of bacteria (E. coli and B. subtilis) while that of Armeniacae Semen showed mild, and that of Persicae Semen showed no effect. Against A. niger, a strong antifungal activity was observed with Armeniacae Semen and only mild activities with Persicae Semen and Mume Semen. None oh the three inhibited the growth of S. cervisiae. The above results may possibly be suggestive of the correlationships between the contents of amygdalin and benzaldehyde in the test extracts with the antimicrobial potencies.

• 미생물학회지
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• 제19권3호
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• pp.108-114
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• 1981

In order to isolate microorganisms which produce antibiotics aganist wheat black rust, some bacteria, molds, and actinomycetes were isolated from soils and screened for the production of antibiptics against wheat black rust. Beacuse wheat black rust-puccinia graminis--is a complete parsitic mold which can't grow in artifical medium, new method for the screening of antibiotic producing microorgsnisms against wheat black rust developed by using live leaves of wheat. With new method, a strain No. $480HS_{20}$ which produces a substnace having strong and Puccinia graminis activity and very narrow antimicrobial spectrum was isolated. the substance produced by the strain No.$480HS_{20}$ had better anti Puccinia graminis activity than any other known antifungal antibotics such as kasurgamycin, balasticidins, actidione, antimycin, ologomycin. And the substance was observed to be very stable at heat and ultraviolet light. The strain was indentified as Bacillus subtilis.