• Journal of Microbiology and Biotechnology
• /
• v.9 no.2
• /
• pp.206-212
• /
• 1999

The gene orf7(oxi III) was expressed using an E. coli system in anticipation that it would encode dTDP-glucose 4,6-dehydratase which is involved in the biosynthesis of the olivose moiety of chlorothricin produced from Streptomyces antibioticus Tu99. The solubility of the expressed protein increased up to 20% under optimal induction conditions. The expressed protein was purified from the E. coli BL 21(DE3) cell lysate by a 28.5-fold purification in two chromatography steps with a 38% recovery to near homogeneity. The molecular weight and N-terminal amino acid sequence of the purified protein correlated with the predicted mass and sequence deduced from the orf7 gene. The purified protein was a homodimer with a subunit relative molecular weight of 38,000 Dalton. The expressed protein was found to exhibit dTDP-glucose 4,6-dehydratase activity and be highly specific for dTDP-glucose as a substrate. The values of K'm and V'max for dTDP-glucose were 28 $\mu$M and 295 nmol $min^{-1} (mg protein)^{-1}$, respectively. dTTP and dTDP were strong inhibitors of this enzyme.$NAD^+$, the coenzyme for dTDP-glucose 4,6-dehydratase, was tightly bound to the expressed protein.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
• /
• v.20 no.3
• /
• pp.140-145
• /
• 2009

Objectives : This study explored the differences in their ego defense mechanisms between adolescents with conduct disorder and normal adolescents. Methods : Subjects were 35 adolescents with conduct disorder and 44 normal adolescents. The Ewha Defense Mechanism Test (EDMT), consisting of 200 items and 20 scales, was administered, to examine the defense mechanisms of both groups of adolescents. Results : Normal adolescents presented statistically significantly higher scores on the reaction formation, controlling, suppression, anticipation, dissociation, and distortion scales than did adolescents with conduct disorder. Zn addition, adolescents with conduct disorder used neurotic defense mechanisms of both neurotic and mature levels less frequently than normal adolescents did. Factor analysis revealed that, normal adolescents had higher scores on ego-expansive factor scales and behavior control factor scales thanadolescents with conduct disorder did. Conclusion : The results suggest adolescents with conduct disorder use mature and ego-expansive defense mechanisms less frequently than do normal adolescents.

• International Journal of Advanced Culture Technology
• /
• v.8 no.3
• /
• pp.300-306
• /
• 2020

This study analyzes the distributor's inventory model in a two-stage supply chain consisting of the supplier, the distributor and the end customer. The supplier will allow a credit period before the distributor settles the account with him in order to stimulate the demand for the product he produces. It is also assumed that the distributor pays the shipping cost for the order and hence, the distributor's ordering cost consists of a fixed ordering cost and the shipping cost that depend on the order quantity. The availability of the delay in payments from the supplier enables discount of the distributor's selling price from a wider range of the price option in anticipation of increased customer's demand. As a result, the availability of a credit transaction leads to an increase in inventory levels. On the other hand, in the case of deteriorating products in which the utility of the product perish over time, the deterioration rate with time plays a role in reducing inventory levels. In this regard, we analyze the effect of the length of the credit period and the degree of product deterioration on the distributor's inventory level. For the analysis, we formulate the distributor's annual net profit and analyze the effect of the length of credit period and deterioration rate of the product on inventory policy numerically.

• Corrosion Science and Technology
• /
• v.6 no.3
• /
• pp.90-95
• /
• 2007

Cathodic protection technology has been widely used on ship's outer hull and inner side of ballast water tanks as a supplementary corrosion protection measure in combination with protective organic coatings. Impressed current cathodic protection system is typically opted for the ship's hull and, sacrificial anode system, for ballast water tanks. The anticipation and interest in cathodic protection system for ships has been surprisingly low-eyed to date in comparison with protective coatings. Computational analysis for the verification of cathodic protection design has been tried sometimes for offshore marine structures, however, in commercial shipbuilding section, decades old design practice is still applied, and no systematic or analytical verification work has been done for that. In this respect, over-rotection from un-erified initial design protocol has been also concerned by several experts. Especially, it was frequently reported in sacrificial anode system that even after full design life time, anode was remaining nearly intact. Another issue for impressed current system, for example, is that the anode shield area design for ship's outer hull should be compromised with actual application situation, because the state-of-the-art design equation is quite impractical from the applicator's stand. Besides that, in this study, some other critical design issues for sacrificial anode and impressed current cathodic protection system were discussed.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2003.07a
• /
• pp.43-49
• /
• 2003

Low-temperature polysilicon (LTPS) technology has continued to mature with the passing of each year since LTPS mass production began. The integration of complex circuits has become possible with advances in microprocessing, leading to the realization of panels with highly advanced functions. At the same time, efforts have been made to meet market demands for lower costs, thereby boosting competitive strength. Today, LTPS-TFT LCDs have become standard equipment for the monitors of digital still cameras, and inroads are being made into the massive cellular phone market. Micro displays such as electronic viewfinders, which were previously only possible with high-temperature polysilicon technology, can now also be made with LTPS, thus expanding the scope of the technology. AMOLED displays using the LTPS-TFT as a back plane are also approaching the stage of industrialization. The hidden potential for the OLED to replace the familiar LCD has prompted Widespread anticipation for this emerging technology. This paper reflects on the history of LTPS technology, then looks forward to its future prospects and suggests a variety of potential fields of application.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.13 no.1
• /
• pp.8-15
• /
• 2014

This study investigates structural and vibration analyses for three types of bike frame fork models. As long as the maximum equivalent stresses of these models are lower than the yield stress, the three models are considered to be safe structurally. Type 3, with a maximum equivalent stress of 169.23 MPa, has the lowest stress among the three models and the strongest strength. Types 1, 2 and 3 have natural frequencies lower than 270 Hz. Type 3, with a critical frequency of 118 Hz, has the best durability under vibration among the three models. In order to decrease the vibration transmitted to a bike rider riding on a rough road, the impact due to vibration can be relieved by selecting a Type 3 model from among the three models. The results of this study can be effectively utilized for the design of a bike frame fork as this allows the anticipation and prevention of damage caused by durability issues.

• Journal of Korean Society of Transportation
• /
• v.21 no.6
• /
• pp.67-73
• /
• 2003

This study preformed a research about the traffic signal light that is adding supplementary green light to prevent driver's cognitive errors and plan safe driving through improving visual cognitivity of present traffic signal light. The result of comparing the present traffic signal light(three colors, four colors) with the traffic signal light(three colors, four colors) adding supplementary green light through car simulator has a significant difference. This result shows possibility that the traffic signal light adding supplementary green light can contribute in safety driving at a point that the traffic signal light advances a point of braking time when drivers didn't recognize by themselves. The findings in this study ca say that there is the meaning in showing a actual application possibility of this study finding by investigating a action of subjective response the moment compare driver's actual response.

• The Bulletin of The Korean Astronomical Society
• /
• v.43 no.1
• /
• pp.46.3-47
• /
• 2018

We present a general N-body exasolar system simulator in anticipation of upcoming searches for exoplanets and even exomoons by next generation telescopes such as James Webb Space Telescope. For habitable zones, traditionally defined by temperature, we here address the essential problem of dynamical stability of planetary orbits. Illustrative examples are presented on P-type orbits in stellar binary systems, that should be fairly common as in Kepler 16b. Specific attention is paid to reduced orbital lifetimes of exoplanets in the habitable zone by the stellar binary, that is propoesed by Maurice van Putten (2017). Especially, we focused on a classic work of complex three-body problem that is well known by Dvorak(1986). We charge his elliptic restricted three-body problem to extend unrestricted three-body problem to look into dynamical motions in view of circumbinary planet, furthermore, we suggest that opposite angular orientation of the planet is relative to the stability of orbits. In here, counter-rotation case is relatively more faster than co-rotation case for being stable. As a result, we find that various initial conditions and thresholds to approach dynamical stability and unstability with unexpectable isolated islands over enormous parameter space. Even, superkeplerian effect of binary is important to habitability of the exoplanet and we can verify that superfaster binary doesn't effect on th planet and increases survivality of planet around the binary.

• Journal of Advanced Navigation Technology
• /
• v.16 no.5
• /
• pp.879-884
• /
• 2012

In accelerating the industrialization, human being have been frequently experiencing man-made crisis such as technology failures, financial problem as well as natural disaster. Emergency management system based on international standard can provide an organization with a framework for continual improvement to increase the possibility of enhancing anticipation, prevention and response as well. In this paper, we introduced the emergency management system design of various organizations for societal security network. In the first, the main role of ISO/TC223 is reviewed and then, current issues of under going standardization in ISO are also addressed. Finally, we have designed a efficient disaster prevention system to minimize the potential risk as well as to improve the organizational resilience.

• Journal of the Korea Furniture Society
• /
• v.27 no.2
• /
• pp.80-90
• /
• 2016

The idea of Sustainable, first mentioned in "Our Future (Bruntdland)" from the United Nations report released in 1987 has been highly affect design disciplines in developed countries in 21st century. It relocated industrial society and structure's attentions to nature and recourse conservation and reuse. Designed and manufactured products eventually disposed and it leads to waste of resources as well as creates environmental issues. Unfortunately, vast majority of designers starts designing without anticipation of this matter and produce more believing this leads to more sales. However shorter life of a product will create bigger increase of resource consumption and waste than, importance of re-usage and recycle will eventually increase. In modern furniture industry metal and plastics are frequently used beside wood that material has been diverse than in past. However, there are lack of plans and regulations of re-usage of those material due to difficulties of separating each different materials and collecting due to it's size. This study would like to look into recycling rate, methods and usage of three major materials (wood, metal, plastic) that is typically used in current furniture industries. Furthermore, see the potential of sustainable furniture by analyzing furniture practices made by each different recycled material from old furniture and what designers should consider to achieve feasibility of sustainable furniture.