• Journal of Applied Biological Chemistry
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• 제60권1호
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• pp.61-68
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• 2017

본 연구에서 층꽃나무(Caryopteris incana Miq.)는 항산화, 피부미백 및 주름개선 활성을 측정하는 데에 사용되었다. 층꽃나무의 생리활성은 고형분 함량보다 첨가된 total phenolic compounds의 농도에 의해 영향을 받는 것으로 확인되어 이후 모든 실험은 층꽃나무로부터 추출한 물과 80 % ethanol 추출물의 total phenolic compounds 함량을 조절하여 실시하였다. 층꽃나무 추출물의 항산화 활성은 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), antioxidant protection factor (PF), Thiobarbituric acid reactive substances (TBARs)를 측정하였다. $200{\mu}g/mL$의 농도에서 물과 ethanol 추출물로 DPPH free radical 소거능을 측정한 결과 각각 84, 92 %의 활성을 나타내었고, ABTS radical 소거능은 물과 ethanol 추출물 둘 다 약 99 %의 활성을 나타내었다. PF를 측정한 결과 물과 ethanol 추출물이 각각 1.56, 1.67 PF 값을 나타내었고, TBARs는 각각 62, 82 %의 활성을 나타내었다. 주름개선 및 피부미백 활성을 측정하였을 때, $200{\mu}g/mL$의 농도에서 80 % ethanol 추출물이 물 추출물보다 눈에 띄게 높은 효과를 나타내었다. 주름개선과 연관된 elastase와 collagenase 저해활성은 ethanol 추출물에서 각각 58, 89 %로 측정되었다. 피부미백과 연관된 tyrosinase의 저해활성은 ethanol 추출물에서 13 %로 측정되었고, 수렴효과는 50 %로 측정되었다. 이러한 결과로 보아, 층꽃나무 추출물은 항산화 활성, 주름개선 및 미백효과가 우수하므로 기능성 화장품 원료의 새로운 자원이 될 것으로 기대되었다.

• KSBB Journal
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• 제28권6호
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• pp.387-393
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• 2013

In this study, the domestic onion skin has been used to investigate the anti-wrinkle effect of the ethanolic extract. The onion skin has been known to contain a large amount of antioxidant including quercetin. Solvent extraction by 80% ethanol was carried out of the onion skin. The high amount of quercetin have been identified using HPLC as a bioactive compound. The extraction yield of the quercetin increased according to temperature up to $80^{\circ}C$. Two different antioxidant assays (DPPH and ABTS) were performed to determine the free-radical scavenging activity of onion skin extracts. The antioxidant activities ($EC_{50}$ of DPPH) of the vitamin C and the onion extracts were 2.2 and $5.09{\mu}g/mL$, respectively. The results of ABTS tests were 2.33 (of vitamin C) and $2.40{\mu}g/mL$ (of the onion extracts), respectively. In a clinical study, a panel of 10 female volunteers between the ages of 30 and 40 (mean age: 42 years) was given a mask pack containing 1% of this extracts for 4 weeks. It was shown that a highly significant reduction in the deep and moderate wrinkled was obtained with those treated with this onion extracts as compared to the control group. The reduction in deep wrinkles increased with time. Eye wrinkle were reduced statistically significant (p<0.05). The application of the proper topical cream and mask pack containing onion skin extracts has therefore shown to be effective enhancing anti-wrinkle activities.

• 대한화장품학회지
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• 제30권2호
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• pp.221-225
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• 2004

인삼(Panax ginseng C. A Meyer)의 뿌리는 전통적인 항-노화 및 항-주름제로 동양에서 사용되어 왔다. 그러나 인삼의 어떤 성분이 주름 형성을 억제하는데 효과적인지는 아직 밝혀지지 않았다. 최근 인삼의 주요 활성 성분으로 생각되는 ginsenosides가 20가지 이상 분리되었다. 이들 중 본 연구원들은 인삼에 의한 항-주름의 작용기작을 밝히기 위해 세포간질(extracellular matrix, ECM) 물질대사에 있어 ginsenoside Rg3의 진피에서의 효과를 시험하였다. 본 연구에서, ginsenoside Rg3의 항-주름 효과를 연구하기 위해 진피의 세포간질 구성 성분과 성장 인자를 ELISA (enzyme-1in14ed immunosorbent assay) 측정법으로 평가하였다. Ginsenoside Rg3은 human dermal fibroblasts 배양에서 type I procollagen과 fibronectin(FN) 생합성을 농도 증가에 비례하여 촉진시키고(p < 0.05, n=3), 농도에 비례하여 TGF-$\beta$1 수준을 증가 (p < 0.05, n=3) 시키는 것으로 밝혀졌다. RT-PCR 분석에서 AP-1 전사 인자(transcription factor)의 일부인 c-Jun의 mRNA 수준이 human dermal fibroblasts에서 ginsenoside Rg3에 의해 감소되었다. 이들 결과들은 ginsenoside Rg3이 fibroblasts에서 TGF-$\beta$1과 AP-1의 발현을 변화시킴으로써 type I collagen과 FN합성을 촉진시킴을 보여준다.

• 한방안이비인후피부과학회지
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• 제23권3호
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• pp.42-65
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• 2010

Objectives : Betula platyphylla var. japonica extract (BPE) was used to determine the modulation of cytokine secretion, the activation of inflammatory and allergic factor and the inhibition of gene expression. Inflammatory and allergic cytokines as IL-$1{\beta}$, IL-2, IL-4, IL-5, IL-6, IL-8, TNF-${\alpha}$, NO and COX-2 were measured to use effectively on improvement or treatment of atopic dermatitis. Methods : We used NC/Nga mouse induced by atopic dermatitis to observe the effects of BPE on the weight, water and feed, blood test, weight of organs, histological change, total IgE and histological change of main organs. Results : BPE is effective on anti-inflammatory and allergic reaction. However, further study is needed to prove which component of BPE indicates effective pharmacological action. Conclusions : The above results suggest that Phellinus igniarius Quel extract could be applicable for improvement of several skin functions.

• 한방안이비인후피부과학회지
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• 제23권3호
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• pp.11-32
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• 2010

Objective : The aim of this study is to determine the effects of Dendrobii herba extract and Punica granatum extract on skin disease and skin beauty. Methods : To investigate in vitro anti-oxidant activity assay, ethanol extracts of medicinal plants tested by DPPH radical, xanthine oxidase activity. In the next experiment, to investigate anti-inflammatory activity assay, examined by relations in NO synthesis, IL-$1{\beta}$, IL-6, TNF-${\alpha}$, NF-${\kappa}B$, COX-2, MAP kinase. To study Skin wrinkle formation effect, we were examined by tyrosinase activities, melanin synthesis in MNT-1 cell. Results : 1. In an anti-oxidant test, Dendrobii and Punica granatum extract showed high radical scavenging activity. 2. In an anti-inflammatory test, Dendrobii herba and Punica granatum extract weakly inhibited the lipopolysaccharide(LPS)-induced nitric oxide(NO) release from RAW 246.7 macrophage cells. Dendrobii herba and Punica granatum extract also inhibited LPS-induced IL-$1{\beta}$ and COX-2 expressions. The inhibitory effect of Dendrobii herba and Punica granatum extract on macrophage activation were via the inhibition of NF-${\kappa}B$, evidenced by transient transfection assay. however, Dendrobii herba and Punica granatum extract did not have any effects about activation of Jun-N-terminal kinase(JNK) and inhibition of p38 MAP kinase in RAW 264.7 cells. 3. In the skin wrinkle formation assay, Dendrobii herba and Punica granatum extract weakly inhibited collagenase and elastase, however it was not statistically significant. 4. In the skin whitening assay, Dendrobii herba and Punica granatum extract weakly inhibited tyrosinase activity, however, it was not statistically significant. They did not have any effect on melanin synthesis, indicating that they could not be applicable for skin whitening. Conclusion : Dendrobii herba extract and Punica granatum extract may play a significant role in skin disease and skin beauty.

• 한방안이비인후피부과학회지
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• 제24권2호
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• pp.68-78
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• 2011

Objective : The purpose of this clinical research was to investigate the effects of herbal cosmetics containing Momordica charntia L. extracts on the anti-wrinkle and whitening effects. Methods : A total of 25 subjects who visited Daegu Hanny Oriental Medical Center from November 1st, 2010 to December 31th, 2010 were included in this study. In this study, we observed change of R1, R2, R3, R4, R5 for using products, change of average melanin content and pigmental area, analysis results of subjective wrinkle improvement, Analysis results of subjective whitening improvement. Statistical analysis was performed by using paired t-test and wilcoxon signed ranks test. Statistical significance was achieved if the probability was less than 5%(p<0.05) Results : Statistically, herbal cosmetics containing Momordica charntia L. extracts showed siginificant effect on change of R1, R2, R3, R4, R5 for using products, change of average melanin content and pigmental area, analysis results of subjective wrinkle improvement, Analysis results of subjective whitening improvement(p<0.05). And satisfaction after using herbal cosmetics was near good. Conclusions : Considering the above results, we have concluded that herbal cosmetics containing Momordica charntia L. extracts have the remarkable effects on the anti-wrinkle and whitening effects.

• 한방안이비인후피부과학회지
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• 제23권3호
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• pp.33-41
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• 2010

Background & Objective : This study was performed to find a new anti-aging, anti-wrinkle agent. Ten kinds of hers were evaluated by proliferation, cytotoxicity, collagen synthesis tests in cultured human dermal fibroblast. Methods : MTT assay was carried out to check cell proliferation, cytotoxic effect of each herbal medicine. Collagen synthesis was investigated by the amount of propeptide using procollagen type-Ⅰ C peptide(PIP) ELISA kit(Takara). Results and Conclusions : There are no cytotoxicity in all group herbs by MTT assay. Collagen synthetic ability was greatest in LYCII FRUCTUS(LF) and was excellent in order of Rehmanniae Radix Preparat(RRP) > Nelumbinis Semen(NS) > Asparai Radix(AR) > Schizandrae Frucus(SF).

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.611-616
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• 2003

Korean ginseng(Panax Ginseng C.A. Meyer) known as a oriental miracle drug is an important medicinal plant. Ginseng has been used for geriatric, tonic, stomachic, and aphrodisiac treatments for thousands years. Also, it is an antibiotic and has therapeutic properties against stress and cancer. Ginseng is widely distributed all over the world. Among them, Korean mountain ginseng has the most valuable effect on pharmaceuticals. The roots of mountain ginseng contained several kinds of ginsenosides that have many active functions for the human body. However, the study of mountain ginseng has a limit because the mountain ginseng is very expensive and rare. So, we artificially cultured mountain ginseng adventitious roots using the bioreactor culture system. We induced callus from original mountain ginseng, directly dug up in mountain and aged about one hundred ten years. Separated adventitious roots were precultured in 500ml conical flasks and then, transferred in 20L bioreactors. The adventitious roots of mountain ginseng were harvested after culturing for 40days, dried and then, extracted with several solvents. In this study, we investigated the whitening effect, anti-wrinkle effect and the safety of tissue cultured adventitious roots extract of mountain ginseng in order to identify the merit as a cosmetic ingredient. Particularly, extract of mountain ginseng adventitious roots showed whitening and anti-wrinkle effects. The inhibitory effect of this extract on the melanogenesis was examined using B-16 melanoma cell. When B-16 melanoma cells were cultured with adventitious root extract, there was a dramatically decrease in melanin contents of 8-16 melanoma cell. And we identified this extract inhibited Dopa auto-oxidation significantly. Also, when transformed mouse fibroblast L929 cells were treated with this extract, there was a significant increase in collagen synthesis. The results show significant inhibited melanization and wrinkle without inhibiting cell viability.

• 대한화장품학회지
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• 제36권4호
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• pp.281-288
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• 2010

본 연구는 에탄올 농도별(0, 30, 50, 70, 100 %)로 추출한 사매추출물의 항산화 효과를 Oxygen Radical Absorbance Capacity(ORAC)로 평가한 결과 30 % 에탄올 추출물에서 가장 우수하였다. 따라서 30 % 에탄올 추출물의 주름 완화에 관련한 효능 및 효과를 확인하기 위하여 마우스 유래 섬유아세포에 대한 세포 독성, MMP-8 collagenase 억제 활성 검사를 실시하였다. 그 결과 30 % 에탄올 사매추출물을 섬유아세포에 처리한 결과 세포 독성이 없었고, 20 %의 MMP-8 collagenase 억제 활성을 보였다. 사매 추출물 1 %가 함유된 Di-Wrinkle Free Cream (DWFC)으로부터 35 ~ 50세의 21명 여성을 대상으로 하루에 2번 도포하여 8주간 인체 안전성과 주름개선 임상평가를 수행하였다. 주름 평가 방법은 3차원 입체 측정기(PRIMOS)를 이용하여 평가를 하였다. DWFC에 대한 안전성 평가는 피험자 방문 시 마다 이상반응, 부종, 인설, 가려움, 자통, 작열감, 뻣뻣함, 따끔거림이나 다른 이상반응이 발생하는지 육안평가와 문진으로 평가하였다. PRIMOS(GFM, Germany)를 이용하여 눈 주변의 주름을 분석한 결과 시험제품 사용 8주 후 통계적으로 유의하게 주름이 13 % 감소하였으며, 시험기간 동안 특별한 피부 이상반응은 관찰되지 않았다. 이와 같은 결과로 보아 사매추출물은 인체에 부작용이 없는 주름개선에 효과 있는 화장품으로 개발이 가능할 것으로 사료된다.

• 한국응용과학기술학회지
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• 제34권3호
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• pp.657-665
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• 2017

본 연구는 화장품 소재로서 연근과 우절의 가능성을 확인하기 위한 것이다. 이를 위해 우리는 연근과 우절 에탄올 추출물을 사용하여 항산화, 항염증 및 항주름에 대한 생물학적 활성 평가를 수행하였다. 연근과 우절을 95% 에탄올로 추출한 다음 항산화 평가를 위해 샘플을 농도 (100, 500, 1000) ${\mu}g/mL$에 따라 처리하여 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거능과 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 양이온 라디칼 소거능을 확인하였다. 또한 항주름 효과를 평가하기 위해 Elastase 저해 활성 평가를 수행 하였다. 항염증 효과를 평가하기 위해서 대식세포 (Raw 264.7 cells)를 이용해 MTT assay를 통한 샘플의 독성평가와 nitric oxide 생성 저해 활성을 평가하였다. 그 결과 연근과 우절의 $1000{\mu}g/mL$ 농도에서 DPPH 라디칼 소거능 활성이 각 66.7%, 99.5% 로 $ABTS^+$ 라디칼 소거능 활성은 동일농도에서 각 51.2%, 98.3%로 나타났다. elastase저해 활성 결과 우절이 연근에 비해 높은 항주름 효능을 나타내었다. 우절은 $1000{\mu}g/mL$ 농도에서 대조군 EGCG 보다 높은 활성을 나타내었다. Nitric oxide 저해 활성 결과에 따르면 연근은 55.8%의 효과를 나타내었고 우절은 66.6%의 효과를 각각 나타내어 우절 추출물이 연근보다 항염증 효과가 더 우수함을 나타내었다. 결과적으로 연근 및 우절 추출물은 안전한 항산화 항염증 및 항주름 천연화장품소재로 사용될 수 있음을 시사한다.