• Proceedings of the Korean Society of Applied Pharmacology
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• 2007.11a
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• pp.140-152
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• 2007

Hyaluronic acid(HA) is a naturally occurring mucopolysaccharide composed of the repeating disaccharide unit of D-glucuronic acid and N-acetyl-D-glucosamine. HA exists as a high molecular weight polymer in the extracellular matrix of many tissues in the body. HA has been widely used not only for osteoarthritis and ophthalmology but also for cosmetics for skin care. Recently HA has drawin much attention for use in health foods. Bioland Corporation started production of HA t1y biotechnological process in 1990 for cosmetical use. At present Bioland produces HA for cosmetic and food use. We have done many researches to evaluate the helpful effect for skin and safety of HA as food. In order to evaluate the effect of HA on the skin after its oral intake, we measured its bioavailability, skin-care effects on anti-wrinkle, moisturization elasticity and desquamation by clinical trial. The results showed that HA is very safe and could bs helpful to improve anti-wrinkling, moisturization, elasticity, and desquamation on the skin.

• Journal of Convergence for Information Technology
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• v.12 no.1
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• pp.144-152
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• 2022

The Spartina anglica is recognized as a highly invasive plant and active eradication methods are required. In this study, we aimed to determine the physiologic activities of Spartina anglica extracts as a cosmetic ingredient. Antioxidant properties were investigated by different chemical methods including radical quenching (DPPH and ABTS), reducing power(FRAP) assay and aerial part of S. anglica(SAA) extract presented the strongest antioxidant activities. The significant cytotoxicity was not observed up to a concentration of 0.5 mg/mL in RAW264.7 cells and NHDF cells. The anti-inflammatory activity of S. anglica belowground(SAB) extract had strong effects on cell-based systems, including LPS-induced NO and cytokines(TNF-a and IL-6) production in RAW264.7 cells. Collagen synthesis and skin hydration gene expression of S. anglica extract showed the highest anti-wrinkle and moisturizing effect in NHDF cells. Results presented in this study tend to show that the ethanol extracts of S. anglica could be exploited as useful-bio-resource for bioactive substances in functional cosmetics.

• Archives of Pharmacal Research
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• v.25 no.4
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• pp.469-474
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• 2002

The inner shell of the chestnut (Castanea crenata S. et Z., Fagaceae) has been used as an anti-wrinkle/skin firming agent in East Asia, and preliminary experiments have found that a 70% ethanol extract from this plant material can prevent cell detachment of skin fibroblasts from culture plates. In order to examine the molecular mechanisms underlying this phenomenon, its effects on the expression of adhesion molecules, such as fibronectin and vitronectin, were investigated using the mouse skin fibroblast cell line, NIH/3T3. Using fixed-cell ELISA, Western blotting and immunofluorescence cell staining, it was clearly demonstrated that the chestnut inner shell extract enhanced the expression of the cell-associated fibronectin and vitronectin. Scoparone (6,7-dimethoxycoumarin), isolated from the extract, also possessed similar properties. These findings suggest that the enhanced expression of the adhesion molecules may be one of the molecular mechanisms for how the chestnut inner shell extract preventing cell detachment and may be also responsible for its anti-wrinkle/skin firming effect.

• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1401-1409
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• 2024

Postbiotics have various functional effects, such as antioxidant, anti-inflammatory, and anti-obesity. Levilactobacillus brevis BK3, the subject of this study, was derived from lactic acid bacteria isolated from Kimchi, a traditional Korean fermented food. The antioxidant activity of BK3 was confirmed through the measurements of 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and total antioxidant capacity (TAC). The wrinkle improvement effect was validated by assessing elastase inhibitory activity and collagenase inhibitory activity. The intracellular activity was confirmed using human keratinocytes (HaCaT) and human fibroblasts (HFF-1). BK3 protects skin cells from oxidative stress induced by H₂O₂ and reduces intracellular reactive oxygen species (ROS) production. In addition, the expressions of the antioxidant genes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were upregulated. Meanwhile, matrix metalloproteinase-1 (MMP-1) and collagen type I alpha 1 (COL1A1), involved in collagen degradation and synthesis, were significantly regulated. These results suggest the possibility of utilizing BK3 as a functional ingredient with antioxidant and wrinkle-improving effects.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.22 no.3
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• pp.36-46
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• 2009

Objective : Recently, the demands for the effective and safe depigmentating and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The aim of this study is to search new materials from the traditional herbal medicines which inhibit the aging process of skin in vitro. Methods : herbal medicines(80% ethanol or water extracts) were screened for their inhibitory activities against elastase. Results : Rheum undulatum (final concentrstion 1 mg/ml) appeared over 30% of inhibition of elastase activity. so we are investigated anti wrinkle effects of Rheum undulatum look through MMP-1 inhibition activity. also Extracts of Rheum undulatum showed higher anti tyrosinase activity than arbutin (final concentration 1 mg/ml). Conclusion : These results suggest that herbal medicines could be strong potential sources of inhibition of anti-aging and whitening effects for the skin.

• Journal of Applied Biological Chemistry
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• v.57 no.4
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• pp.365-371
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• 2014

In the present study, we investigated the biological activities of Xylosma congesta leaf ethanol extract (XCO) using a variety of in vitro and cell culture model systems for anti-melanogenic, anti-wrinkle, anti-inflammatory and anti-oxidant activities. First, XCO markedly inhibited ${\alpha}$-melanocyte stimulating hormone-stimulated melanin synthesis in B16F10 cells. Secondly, XCO marginally induced procollagen synthesis in CCD-986SK cells. Thirdly, XCO dose-dependently suppressed lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 cells. XCO did not affect cell viability at different concentrations used in this study, indicating that XCO-mediated inhibition of melanin, procollagen and NO synthesis is not mediated by cytotoxicity. Finally, XCO was found to exert anti-oxidant effect. Taken together, these findings demonstrate for the first time that XCO possesses anti-melanogenic, anti-wrinkle, anti-inflammatory and anti-oxidant activities, and suggest further evaluation and development of XCO as a functional supplement or cosmetic that may be useful for whitening skin, reducing wrinkles and treating inflammatory responses.

• Journal of the Korea Convergence Society
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• v.12 no.1
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• pp.251-257
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• 2021

ln this study, natural extracts extracted from cypress sapiens, a natural material, were investigated as materials that could protect skin aging caused by ultraviolet rays, and experiments were conducted on the synthesis of filaggrins that make up the natural moisturizing factor of the skin, the synthesis of pro-colagen, a fibrous protein, which plays an important role in moisturizing the dermis, and elastin, which is an enzyme that decomposes collagen. As a result, cypress ethanol extract (COE) was a dependent inhibitor to collagenase and elastase, inhibiting the synthesis of filaggrin and the expression of MMP-1 for exfoliated cells damaged by ultraviolet rays. Therefore, it is estimated that ethanol extract will have the effect of delaying wrinkles and as a functional cosmetic material that inhibits skin aging convergence. Based on this study, we would like to further study the mechanism of the synthesis of filaggrin on the suppression of expression of MMP, which is the anti-wrinkle effect.

• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1567-1573
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• 2020

Ultraviolet (UV) is one of the major factors harmful to skin health. Irradiation with ultraviolet accelerates the decline of skin function, causing the skin to have deep wrinkles, dryness, decreased procollagen production, and degradation of collagen. Novel materials are needed to prevent the aging of the skin by blocking the effects of UV. Safflower seed oil (Charthamus tinctorius L., SSO) contains significantly high levels of unsaturated fatty acids and phytochemicals. SSO has been traditionally used in China, Japan, and Korea to improve skin and hair. Our objective in this study was to determine the effect of SSO and its active compound acacetin on UVB-induced skin photoaging in HaCaT cells and human dermal fibroblasts (HDF). SSO inhibited UVB-induced matrix metalloproteinase-1 (MMP-1) at both protein and mRNA levels in HaCaT cells and HDF. MMP-1 is known to play important roles in collagen degradation and wrinkle formation. Acacetin, a type of flavonoid, is present in SSO. Similar to SSO, acacetin also inhibited UVB-induced MMP-1 protein and mRNA levels in HaCaT cells and HDF. MMP-1 mRNA is primarily regulated by the mitogen-activated kinase (MAPK) signaling pathway. Acacetin regulated the phosphorylation of JNK1/2 and c-jun, but did not inhibit the phosphorylation of ERK1/2, p38 and AKT. Taken together, these results indicate that SSO and its active compound acacetin can prevent UVB-induced MMP-1 expression, which leads to skin photoaging, and may therefore have therapeutic potential as an anti-wrinkle agent to improve skin health.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.49-57
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• 2018

In this study, we investigated the anti-aging effect of Rosa damascena extract containing low molecular glycoprotein (RELG) converted from the high molecular glycoprotein by bioconversion. Free radical scavenging activities were performed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Antioxidant activities ($IC_{50}$) of RELG and the positive control ascorbic acid were $22.6{\mu}g/mL$ and $21.1{\mu}g/mL$, respectively. For skin cells, $15{\mu}g/mL$ RELG showed 28% antioxidant activity by inhibiting the production of active oxygen species induced by ultraviolet ray and hydrogen peroxide. $15{\mu}g/mL$ RELG prevented 10% the cell death caused by stress in human hair follicle dermal papilla cells (HDPC) and reduced 90% the production of active oxygen species. In addition, the glycoprotein showed not only anti-wrinkle effect but also moisturizing effect by 48% inhibition of matrix metallo proteinase-1 (MMP-1) production by ultraviolet stress and $10{\mu}g/mL$ RELG enhanced 10% neutral lipid synthesis with 44% aquaporin 3 (AQP3) expression, which is moisture factor. In conclusion, the RELG can be used as an anti-aging cosmetic material.

• YAKHAK HOEJI
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• v.51 no.5
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• pp.343-349
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• 2007

To investigate the effect of ethanol extract of Saxifraga stolonifera MEERBURGH on skin care, we measured anti-oxidant and anti-aging activity. S. stolonifera ethanol extract itself had anti-oxidant activity in a dose-dependent manner in DPPH radical scavenging. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. S. stolonifera ethanol extract inhibited silica-induced intracellular superoxide anion generation, $H_2O_2$ and hydroperoxide generation in RAW 264.7 cells. S. stolonifera ethanol extract significantly inhibited both hyaluronidase and elastase activity, also significantly inhibited MMP-1(collagenase) activity as well. In NIH 3T3 fibroblast cells, S. stolonifera ethanol extract significantly increased collagen-like polymer synthesis, which suggesting the S. stolonifera ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is suggested that the main ingredients of S. stolonifera ethanol extract play an important role in anti-oxidant and anti-aging activity.