• Journal of Acupuncture Research
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• 제23권2호
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• pp.21-31
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• 2006

In the present study, We have investigated the bee venom (BV) and melittin (a major component of BV)-mediated anti-proliferative effects, and defined its mechanisms of action in cultured rat aortic vascular smooth muscle cells (VSMCs). BV and melittin $(0.4{\sim}0.8\;{\mu}g/ml)$ effectively inhibited 5% FBS-induced VSMCs proliferations. The regulation of apoptosis has attracted much attention as a possible means of eliminating excessively proliferating VSMCs. In the present study, the treatment of BV and melittin strongly induced apoptosis of VSMCs. These results suggest that the anti-proliferative effects of BV and melittin in VSMCs should be related with induction of apoptosis. Further study about Influence of BV and melittin upon apoptosis mechanism is therefor thought to be necessary to confirm the above results.

• 생명과학회지
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• 제26권10호
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• pp.1130-1136
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• 2016

본 연구에서는 꼬시래기 산추출물(acid extraction of Gracilaria verrucosa, AEG)을 이용하여 RC-58T/h/SA#4 primary 인체 전립선 암세포에 대한 증식억제 및 apoptosis 유도효과를 밝히고자 하였다. AEG의 처리는 전립선 암세포에서 24시간에서 농도 의존적으로 증식 억제능을 보이는 반면 정상세포에서는 독성을 나타내지 않아 암세포의 증식만을 선택적으로 억제시킴을 확인할 수 있었다. 또한 RC-58T/h/SA#4 세포에서 AEG의 처리는 apoptotic body 형성 및 핵의 형태 변화를 유도하였으며, anti-apoptotic 인자인 Bcl-2 단백질은 감소시키고 pro-apoptotic 인자인 Bax 단백질은 증가시키는 것으로 나타났다. Apoptosis의 유발과 관련된 주요인자인 caspase-3 단백질의 발현은 대조구와 비교하여 AEG를 처리한 군에서 caspase-3의 발현을 농도 의존적으로 증가시키는 것으로 나타났다. 한편, bisphenol A에 의해 비정상적으로 증식된 전립선 암세포에서 AEG의 처리는 유의적인 전립선암세포 성장억제효능을 나타내었다. 본 연구에서는 AEG가 RC-58T/h/SA#4 전립선암 세포에서 암세포 성장억제효과 및 apoptosis 유도효과를 나타낸다는 것을 확인하였으며, 환경호르몬에 의해 증식된 암에 대해서도 성장을 억제할 수 있는 효능을 가지고 있음을 증명하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2307-2312
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• 2015

Curcumol is a sesquiterpene originally isolated from curcuma rhizomes, a component of herbal remedies commonly used in oriental medicine. Its beneficial pharmacological activities have attract significant interest recently. In this study, anti-cancer activity of curcumol was examined with both in vitro and in vivo models. It was found that curcumol exhibited time- and concentration-dependent anti-proliferative effects in SPC-A-1 human lung adenocarcinoma cells with cell cycle arrest in the G0/G1 phase while apoptosis-induction was also confirmed with flow cytometry and morphological analyses. Interestingly, curcumol did not display growth inhibition in MRC-5 human embryonic lung fibroblasts, suggesting the anti-proliferative effects of curcumol were specific to cancer cells. Anti-neoplastic effects of curcumol were also confirmed in tumor bearing mice. Curcumol (60 mg/ kg daily) significantly reduced tumor size without causing notable toxicity. In conclusion, curcumol appears a favorable anti-cancer candidate for further development.

• 생명과학회지
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• 제22권4호
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• pp.538-544
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• 2012

에탄올은 사람에 대한 발암물질로 잘 알려져 있다. 또한 여러 조직이나 세포에서의 에탄올에 의한 세포증식억제효과도 잘 알려져 있다. 본 연구에서는 여러 암세포에서 에탄올에 의한 세포증식억제 효과를 조사하였는데 특히 발암원성 $ras$로 형질전환되거나 미세주입된 세포에서의 영향을 조사하였다. 에탄올은 여러 정상세포들의 증식을 억제하였다. 반면에 여러 암세포나 발암원성 Ras에 의한 세포증식은 억제하지 못 하였다. 또한 발암원성 단백질의 세포내 미세주사에 의한 DNA합성 유도도 에탄올에 의해 억제 되지 않았다. 이러한 에탄올의 세포증식억제 효과는 $N$-acetylcysteine이나 4-methylpyrazole과 같은 항산화제에 의해 제거되었다. 이러한 실험 결과는 에탄올에 의한 세포증식억제 효과는 Ras단백질의 upstream에 있거나 또는 Ras와 독립적으로 작용하며, 활성산소 형성과 밀접한 관계가 있다는 것을 알려준다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.256.1-256.1
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• 2002

We investigated the anti-proliferative effects of a new compound. ircinin-1. from the sponge Sarcotragus sp. on SK-MEL -2 human skin cancer cells. From the data of MTT assay, cell viability was decreased by ircinin-1 in a dose-dependent manner. We observed that the anti-proliferative effect of ircinin-1 was due to the induction of apoptosis, which was confirmed by observing the morphological changes. the increased ratio of pro-apoptotic protein Bax to anti-apoptotic protein Bcl-2, and cleavage of poly(ADP-ribose) polymerase protein, via activation of caspase-3. (omitted)

• Advances in Traditional Medicine
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• 제4권2호
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• pp.91-94
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• 2004

Water and chloroform/methanol extracts of the three local Malaysian snakehead fish, Channa striatus (striped snakehead), Channa micropeltes (giant snakehead) and Channa lucius (blotched snakehead) were evaluated for inhibitory activity in chronic inflammation, using cotton pellet granuloma test. Both water extracts of C. striatus and C. micropeltes showed marked inhibition of the transudative and proliferative components of chronic inflammation (42.9 and 31.2% respectively for C. striatus, 35.6 and 26.2% for C. micropeltes) when compared to those of mefenamic acid (25.1 and 21.3% respectively) and piroxicam (36.1 and 26.2% respectively). The chloroform/methanol extracts did not exhibit any anti-inflammatory effects. These results indicated that C. striatus has more anti-transudative and anti-proliferative activities than the extract of C. micropletes. C. lucius extract in contrast, did not inhibit these two components. This present study indicated the beneficial effects of the water extracts of C. striatus and C. micropeltes, but not C. lucius on chronic inflammation.

• 생약학회지
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• 제46권2호
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• pp.133-139
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• 2015

Two coumarinolignans, cleomiscosins C (1) and D (2) were isolated from the heartwood of Acer mono, together with four compounds, 5-O-methyl-(E)-resveratrol-3-O-${\beta}$-D-glucopyranoside (3), 5-O-methyl-(E)-resveratrol-3-O-${\beta}$-D-apiofuranosyl-(1$\rightarrow$6)-${\beta}$-D-glucopyranoside (4), scopoletin (5), and (E)-resveratrol-3-O-${\beta}$-D-glucopyranoside (6). Of them, cleomiscosins C (1) and D (2) were applied to preparing inclusion complex molecules with ${\beta}$-cyclodextrin (${\beta}$-CD) to improve the very poor solubility in cell media. The CD complexes of 1 and 2 exhibited an enhancement of water solubility which is feasible to measure their cytotoxicity using a spectrophotometer in a cell-based assay. Anti-proliferative activity of these complex molecules was successfully estimated on HCT116 human colon cancer cells, and cleomiscosin D (2) showed anti-proliferative effects at the concentration of 1.95~31.2 ${{\mu}g}$/mL in a dose-dependent manner.

• Biomolecules & Therapeutics
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• 제32권1호
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• pp.38-55
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• 2024

Cancer is a global health challenge with high morbidity and mortality rates. However, conventional cancer treatment methods often have severe side effects and limited success rates. In the last decade, extensive research has been conducted to develop safe, and efficient alternative treatments that do not have the limitations of existing anticancer medicines. Plant-derived compounds have shown promise in cancer treatment for their anti-carcinogenic and anti-proliferative properties. Rosmarinic acid (RA) and carnosic acid (CA) are potent polyphenolic compounds found in rosemary (Rosmarinus officinalis) extract. They have been extensively studied for their biological properties, which include anti-diabetic, anti-inflammatory, antioxidant, and anticancer activities. In addition, RA and CA have demonstrated effective anti-proliferative properties against various cancers, making them promising targets for extensive research to develop candidate or leading compounds for cancer treatment. This review discusses and summarizes the anti-tumor effect of RA and CA against various cancers and highlights the involved biochemical and mechanistic pathways.

• 한국식품과학회지
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• 제49권3호
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• pp.242-251
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• 2017

본 연구의 목적은 유자씨와 유자씨 유지로부터 HT-29 암세포 생장 억제효과를 확인하고 주요 원인 물질을 확인하는 것이다. 유자씨, 헥산 추출 유자씨 유지, 냉압착 유자씨 유지로부터 60% 메탄올을 이용하여 추출물(각각 ES, EHO, ECO)을 얻었다. 추출물의 성분은 HPLC-MS를 이용하여 확인하였다. ES, EHO, ECO를 HT-29 세포에 처리하여 생장 억제 효과를 확인한 결과, EHO와 ECO가 유의적인 효과가 있었다(p<0.05). 반면, ES와 리모닌, 노밀린은 24시간과 48시간 처리 후에 암세포 생장 억제 효과가 없었다(p>0.05). 유자씨 유지 추출물의 암세포 생장 억제효과에 주요 역할을 하는 성분을 탐색하기 위해 제조용 LC로 EHO와 ECO를 분획하여 이 분획물의 암세포 생장 억제 효과와 조성을 확인하였다. 분획물 중에서 EHO의 3개 분획물과 ECO의 2개 분획물이 유의적인 HT-29 세포 생장 억제 효과가 있었다(p<0.05). 이 5개 분획물의 HPLC-MS 분석 결과, 아이소핌피넬린, 버갑텐, 이찬젠신이 주요 성분일 것으로 추정되었다. 아이소핌피넬린, 버갑텐, 이찬젠신을 HT-29 세포에 처리한 결과, 이찬젠신이 유의적인 생장 억제 효과가 있었고(p<0.05), 아이소핌피넬린과 버갑텐은 약간의 생장 억제 효과가 있었으나 유의적이지는 않았다(p>0.05). 따라서 아이소핌피넬린, 버갑텐, 이찬젠신이 유자씨 유지 내의 주요 암세포 생장 억제 물질이며, 이 중에서 이찬젠신이 그 활성이 가장 높은 물질인 것으로 추정한다.

• Biomolecules & Therapeutics
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• 제19권2호
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• pp.211-217
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• 2011

Panax ginseng CA Meyer, a herb from the Araliaceae, has traditionally been used as a medicinal plant in Asian countries. Ginseng extract fermented by ginsenoside-${\beta}$-glucosidase treatment is enriched in ginsenosides such as Rh2 and Rg3. Here we show that a fermented ginseng extract, BST204, has anti-proliferative and anti-invasive effects on HT-29 human colon cancer cells. Treatment of HT-29 cells with BST204 induced cell cycle arrest at $G_1$ phase without progression to apoptosis. This cell cycle arrest was accompanied by up-regulation of tumor suppressor proteins, p53 and p21$^{WAF1/Cip1}$, down-regulation of the cyclin-dependent kinase/cyclins, Cdk2, cyclin E, and cyclin D1 involved in $G_1$ or $G_1/S$ transition, and decrease in the phosphorylated form of retinoblastoma protein. In addition, BST204 suppressed the migration of HT-29 cells induced by 12-O-tetradecanoylphorbol-13-acetate, which correlated with the inhibition of metalloproteinase-9 activity and extracellular signal-regulated kinase activity. The effects of BST204 on the proliferation and the invasiveness of HT-29 cells were similar to those of Rh2. Taken together, the results suggest that fermentation of ginseng extract with ginsenoside-${\beta}$-glucosidase enhanced the anti-proliferative and the anti-invasive activity against human colon cancer cells and these anti-tumor effects of BST204 might be mediated in part by enriched Rh2.