• 대한한의학방제학회지
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• 제23권2호
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• pp.189-198
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• 2015

Objectives : Sparganii Rhizoma is frequently used in traditional herbal medicine for treatment of blood stasis, amenorrhea and functional dyspepsia and has been reported to exhibit anti-oxidant, anti-proliferation and anti-angiogenesis peoperties. In this study, we investigated the cytoprotective effect and underlying mechanism of Sparganii Rhizoma water extract (SRE) against oxidative stress-induced mitochondrial dysfunction and apoptosis in hepatocyte. Methods : To determine the effects of SRE on oxidative stress, we induced synergistic cytotoxicity by co-treatment of arachidonic acid (AA) and iron in the HepG2 cell, a human derived hepatocyte cell line. Results : Treatment of SRE increased relative cell viability and altered the expression levels of apoptosis-related proteins such as Bcl-xL, Bcl-2 and procaspase-3. And SRE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species production induced by AA+iron. In addition, SRE activated of AMP-activated protein kinase (AMPK), a potential target for cytoprotection, by increasing the phosphorylation of AMPKα at Thr-172. Morever, SRE increased phosphorylation of acetyl-CoA carboxylase, a direct downstream target of AMPK. Conclusion : These results indicated that SRE has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with AMPK pathway.

• 생명과학회지
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• 제31권4호
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• pp.389-399
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• 2021

이 연구는 인간진피섬유아(HDF)세포에서 병풀 및 아시아티코사이드가 H₂O₂ 유래 세포주기 정지기, 미토콘드리아 활성 및 염증성 사이토카인에 미치는 영향을 조사하였다. 병풀 80% 메탄올 추출물, 에틸아세티이트 분획물 및 병풀의 대표물질인 아시아티코사이드를 사용하였다. 병풀 추추물, 에틸아세테이트 분획 및 아시아티코사이드로 처리한 세포는 낮은 수준의 TNF-α 및 IL-6을 분비하였고, 아시아티코사이드의 항산화 효과는 병풀 추출물 및 에틸아세테이트 분획물보다 높았다. 아시아티코사이드 처리는 미토콘드리아의 막포텐셜을 증가시키고, 미토콘드리아를 정상으로 되돌렸다. 스트레스 유도 후 에틸아세테이트 분획물 및 아시아티코사이드에 의해 미토콘드리아 산소 소비율이 증가하였고, TLR4-MyD88-TRAF6-p65 경로가 재감소하였다. 이러한 결과는 병풀 추출물, 에틸 아세테이트 분획 및 아시아티코사이드가 HDF 세포의 미토콘드리아 활성을 조절할 뿐 아니라 항산화 및 항염증에 효과 있음을 시사한다.

• Applied Biological Chemistry
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• 제49권2호
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• pp.135-139
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• 2006

본 논문은 도인(桃仁)(Persicae semen)의 화장품 약리활성으로 항산화 효과, tyrosinase 억제효과, 아질산염 소거능를 살펴보았다. 도인(桃仁)은 도인 전체, 껍질, 껍질을 제외한 알맹이로 각각 나누어 사용하였다. 도인(桃仁) 껍질 열수 추출물과 에탄올 추출물은 1,000 ppm에서 각각 89%, 87%이상의 전자 공여능을 나타내어 같은 농도의 BHA에 비해 높은 효과를 나타내었다. SOD 유사활성실험에서 10,000 ppm의 BHA가 89%, 도인(桃仁) 껍질 열수 추출물은 93%의 효과를 나타내었다. Xanthine oxidase 억제실험에서는 1,000 ppm의 BHA가 27%의 효과를 나타내었고, 도인(桃仁) 껍질 에탄올 추출물은 62%의 효과를 나타내었다. Tyrosinase 억제실험에서 도인(桃仁) 전체, 껍질, 알맹이 에탄올 추출물 10,000 ppm에서 모두 98%이상의 효과를 나타내었다. 1,000 ppm의 도인껍질 에탄올 추출물에서 96%의 아질산염 소거능을 나타내었다. 위와 같은 결과를 통해 도인(桃仁)의 화장품 천연소재로써 응용 가능함을 알 수 있었다.

• 한국식품저장유통학회지
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• 제22권6호
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• pp.878-885
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• 2015

본 연구는 새로운 기능성 소재의 개발에 우선하여 박주가리 잎과 줄기의 열수 및 70% 에탄올 추출방법에 따라 각 추출물에 대한 항산화 활성을 측정함으로써 천연 항산화제 개발에 필요한 기초자료를 얻고자 하였다. 박주가리 잎과 줄기의 열수 및 에탄올 추출물의 수율 측정 결과 박주가리 잎의 열수 추출물은 6.89%으로 다소 높았고, 총 폴리페놀 함량 및 플라보노이드 함량은 박주가리 잎의 에탄올 추출물인 ALEE에서 각각 86.96, 60.73 mg/g으로 가장 높았다. 전자공여능은 박주가리 잎의 열수 추출물 ALWE는 추출물 농도에 따라 36.20~68.19%의 전자공여능을 보여 다른 추출물에 비해 높은 전자공여능을 보였다. SOD 유사활성능은 추출물의 농도가 증가함에 따라 박주가리 추출물 ALWE, ALEE에서 다소 높았다. 아질산염 소거능 측정 결과 pH 1.2에서 3.0에 비해 소거능은 높았으며, 박주가리 잎 열수 추출물인 ALWE에서 높은 아질산염 소거능을 보였다. 환원력 측정 결과 추출물 농도 $62.5{\mu}g/mL$에서는 박주가리 잎 에탄올 추출물 ALEE에서 0.09로 다른 추출물에 비해 다소 높은 환원력을 보였고, 추출물 $1,000{\mu}g/mL$의 농도에서는 박주가리 잎 열수 추출물 ALWE가 0.44로 환원력이 높았다. Tyrosinase 저해활성 측정 결과에서는 $62.5{\mu}g/mL$ 농도에서는 박주가리 줄기 에탄올추출물인 ASEE에서 13.81%로 다소 높았으며, $1,000{\mu}g/mL$의 농도에서는 박주가리 잎의 에탄올 추출물 ALEE에서 57.04%로 높은 저해활성을 보였다. 그리고 박주가리 잎과 줄기 열수 및 에탄올 추출물의 농도가 증가할수록 tyrosinase 저해활성이 높았다.

• 셀메드
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• 제3권3호
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• pp.25.1-25.8
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• 2013

Homoeopathically prepared Condurango 30C is traditionally used in amelioration of certain types of cancer by homeopathic practitioners. In this study, ability of Condurango 30C in amelioration of the conventional benzo[a]pyrene (BaP)-induced lung cancer in rat has been tested. After one month of scheduled oral feeding of BaP, lung cancer is routinely developed after four months in rats. Tumorbearing rats were then treated with Condurango 30C for the next one ($5^{th}$), two ($6^{th}$) and three ($7^{th}$) months, respectively, and sacrificed. Efficacy of post-cancer treatment by Condurango 30C was evaluated against controls (placebo) by different study parameters like: body and lung weights, number and diameter of lung tumour nodules, lung architecture, DNA damage, anti-oxidant activity and reactive oxygen species (ROS) accumulation. Administration of this homeopathic remedy caused increase of body weight and decrease of lung weight, decrease in number and diameter of lung tumour nodules, particularly after one and two months of drug treatment. BaP intoxication significantly increased lipid peroxidase (LPO) with concomitant decrease in activities of different antioxidants, while Condurango 30C administration certainly reduce their levels than normal and cancerous groups, notably after one and two months' of drug treatment. Condurango 30C showed capability to induce ROS-mediated cell death evidenced from the study of ROS activities at different time-points. Further, the remedy possibly achieved its anticancer goal through mediation of DNA-nicks that possibly led cancer cells to the apoptotic pathway. Thus, Condurango 30C has anticancer potential in BaP-induced lung cancer of rats via tissue damage recovery and ROS-mediated programmed cell death.

• 대한한의학방제학회지
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• 제25권2호
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• pp.155-166
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• 2017

Objectives : Polygoni Multiflori Ramulus has been widely used as a traditional medicinal herb for the treatment of insomnia, limb pain and itch. The extract of Polygoni Multiflori Ramulus (PMRE) is known to have a modulatory effect of many inflammatory responses. This study was performed to investigate the hepatoprotective effect of PMRE against arachidonic acid (AA) + iron-induced oxidative stress on HepG2 cell and carbon tetrachloride ($CCl_4$)-induced liver injury on mice. Methods : The effects of PMRE on cell viability was assessed by MTT assay. And flow cytometric analysis was performed to estimate the effects on mitochondrial function. To investigate its underlying mechanism, apoptosis-related proteins were analysed by using immunoblot analysis. In addition, ICR mouse were administrated (po) with the PMRE (30, 100 mg/kg) for 3 days and then, injected (ip) with $CCl_4$ (0.5 ml/kg body weight) to induce acute liver damage. The level of pro-caspase-3 was measured. Results : Treatment of PMRE increased relative cell viability, prevented a cleavage of poly (ADP ribose) polymerase and pro-caspase-3, and also reduced mitochondrial membrane permeability against AA + iron-induced oxidative stress. In addition, PMRE treatment decreased liver injury induced by $CCl_4$, as evidenced by increases in pro-caspase-3 level. Conclusions : These results demonstrate that PMRE has an ability to anti-oxidant and hepatoprotective effect against AA + iron-induced oxidative stress and $CCl_4$-induced liver injury.

• BMB Reports
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• 제40권3호
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• pp.382-395
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• 2007

The herb, Cajanus indicus L, is well known for its hepatoprotective action. A 43 kD protein has been isolated, purified and partially sequenced from the leaves of this herb. A number of in vivo and in vitro studies carried out in our laboratory suggest that this protein might be a major component responsible for the hepatoprotective action of the herb. Our successive studies have been designed to evaluate the potential efficacy of this protein in protecting the hepatic as well as renal tissues from the sodium fluoride (NaF) induced oxidative stress. The experimental groups of mice were exposed to NaF at a dose of 600 ppm through drinking water for one week. This exposure significantly altered the activities of the antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione reductase (GR) and the cellular metabolites such as reduced glutathione (GSH), oxidized glutathione (GSSG), total thiols, lipid peroxidation end products in liver and kidney compared to the normal mice. Intraperitoneal administration of the protein at a dose of 2 mg/kg body weight for seven days followed by NaF treatment (600 ppm for next seven days) normalized the activities of the hepato-renal antioxidant enzymes, the level of cellular metabolites and lipid peroxidation end products. Post treatment with the protein for four days showed that it could help recovering the damages after NaF administration. Time-course study suggests that the protein could stimulate the recovery of both the organs faster than natural process. Effects of a known antioxidant, vitamin E, and a non-relevant protein, bovine serum albumin (BSA) have been included in the study to validate the experimental data. Combining all, result suggests that NaF could induce severe oxidative stress both in the liver and kidney tissues in mice and the protein possessed the ability to attenuate that hepato-renal toxic effect of NaF probably via its antioxidant activity.

• 한국식품조리과학회지
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• 제29권6호
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• pp.653-660
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• 2013

Acaiberry(Euterpe oleracea Mart.) was partially substituted from the formula for juak and the subsequent effects on the physiochemical and physical characteristics. And this study was carried out to standardize the recipe of acaiberry-juak. Acaiberry powder was added in 0(control), 1, 3 and 5% substitution ratios by weight per glutinous rice flour. Also, acaiberry puree was added in 3, 6 and 9% substitution ratios by weight water. The moisture contents showed juak with addition of acaiberry contents were lower then control. The diameter of the juak decreased with increasing levels of the added acaiberry powder and puree, whereas the height increased with increasing amount of the powder and puree. The Hunter colorimeteric L-values of the dough and acaiberry-juak decreased as the amount of acaiberry powder and puree increased. Hunter a and b-values both increased with increasing levels of the acaiberry powder and puree. Textural characteristics there were increase in hardness, springiness, chewiness as the amount of acaiberry powder and puree increased(p<.001). Cohesiveness were not significantly different as the level of acaiberry powder and puree increased(p<.001). The anti-oxidant capacity results, anthocyanin, DPPH radical scavenging activity, SOD-like activity, Nitrate-scavenging ability tended to increased by according to the addition acaiberry powder and puree(p<.001). As measured DSC, the onset temperature and enthalpy were accordingly increased during storage period as the acaiberry contents were higher then control. According to sensory evaluation results, the after swallowing, appearance, flavor, taste and texture of juak was acaiberry powder 1% and acaiberry puree 6% proper score is recorded. Overall acceptability of acaiberry juak powder 1%, puree 6% percent of the additives are also the highest mark in the sensory characteristics. Also aciberry powder addition of more than demonstrate a good reputation in the development juak will suggest more appropriate form of puree.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1026-1032
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• 2001

Microwave 처리가 쌀가공 부산물로 발생하는 왕겨와 미 의 항산화능에 미치는 영향을 조사하였다. 100 W, 200 W, 440 W의 출력으로 microwave를 처리하였을 때, 출력과 처리시간에 비례하여 갈변을 나타내는 색도의 변화가 관찰되었다 그러나, 왕겨와 미강의 전자공여능은 조사된 범위 내에서 microwave처리에 대해 큰 변화를 보이지 않았다. 활성산 소종($H_2O$$_2$,O$_2$$^{-}$, .OH)을 발생시켜 어유의 과산화에 대한 왕겨와 미강의 과산화 억제력을 조사한 결과, 전반적으로 왕겨가 미강보다 microwaye 처리에 안정함을 보였다 특히, $H_2O$$_2$에 대한 왕겨의 과산화 억제력은 미강에 비하여 매우 높게 측정되었다. 이상의 결과로 왕겨가 미강보다 항산화 물질을 많이 함유함으로써 미강보다 높고 안정한 항산화능을 나타내 고, 또한 microwave 처리로 인하여 항산화 물질이 일부 파괴되지만 항산화력을 가지는 갈변 반응 생성물이 생성되어 왕겨의 경우에는 microwave 처리에 대해 영향을 거의 받지 않 음을 알 수 있었다. 따라서, microwave 처리 공정은 왕겨나 미강의 항산화능을 이용한 가공 방법에 이용될 수 있음을 의미한다.

• 한국패션뷰티학회지
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• 제5권4호
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• pp.139-144
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• 2007

There was an increasing interest that herbal medicine and natural material extracts were proved processes of antioxidant, cosmeceutical activity and the other effects. The aim of this study was to assess the antioxidant of extraction of four kinds from Prunus armeniaca L., Reynoutria elliptica, Curcuma aromatica, Lithospermum erythrorhizon. RE (Reynoutria elliptica) and CA (Curcuma aromatica) have good electron donating ability. The water and ethanol extract of RE at a 100 ppm concentration showed over 70%, the water extract at 500 ppm concentration showed 83% and the ethanol extract at 100 ppm concentration showed 86% of CA. Xanthine oxidase inhibition activity of the water extract of LE (Lithospermum erythrorhizon) at a 1,000 ppm concentration showed over 44%, on the other hand, RE showed in all lowest effect and there was no inhibition activity of a couple more extracts. In the measurement of nitrite scavenging activity, all extracts showed highly scavenging activity. Especially the water and ethanol extract of RE showed over 99% at 500 ppm, also LE showed over 40% at 10 ppm concentration.