• 한방비만학회지
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• 제15권2호
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• pp.111-122
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• 2015

Objectives: Schizandrae fructus (Schizandra chinensis) is one of very common herbs, it is known as natural antioxidants, anti-inflammatory agent. Also some reports show that its extract works to regulate of dyslipidemia. This study was designed to investigate the effects of Schizandrae fructus chloroform fraction (SFCF) on serum lipid levels in dyslipidemic mice. Methods: The levels of total cholesterol, high density lipoprotein-cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), fasting blood glucose in serum were measured. Histopathological and gene expression changes in liver tissue were also observed. Results: Oral administration of SFCF lowered levels of total cholesterol and triglyceride, which were elevated by high-fat diet. But SFCF did not affect on weight changes and serum AST, ALT levels in dyslipidemic mice. After carrying out gene ontological analysis, large numbers of genes in high-fat diet group were up-(347) or down-regulated (235). In SFCF treated mice, some changed expression of the genes was restored to normal levels, with a recovery rate of 17%. And it seems that fatty acid biosynthesis pathway was one of important key pathways to recovery. Conclusions: SFCF has beneficial effect on dyslipidemia, and could be used to prevent and treat cardiovascular disease.

• 한국식생활문화학회지
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• 제23권6호
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• pp.812-819
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• 2008

In this study, we assessed the effects of dietary supplementation with Ecklonia cava on blood glucose, lipid metabolism, and renal oxidative stress in streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into a normal rat group fed on a control diet and diabetic rats fed on a control diet or supplemented with powder (15% w/w) or water extract of Ecklonia cava (2.5% w/w). Diabetes was induced by a single injection of STZ (60 mg/kg, ip) in citrate buffer. The animals were fed ad libitum with the experimental diet and water for 5 weeks. Dietary supplementation of Ecklonia cava powder and water extract was shown to reduce blood glucose levels in the diabetic rats, and the water extract was more effective than the powder. Dietary supplementation with Ecklonia cava also reduced LDL cholesterol and increased HDL-cholesterol levels in the diabetic rats. Renal glutathione S-transferase activity was increased in the diabetic rats as compared to the normal rats, but reverted to near control values as the result of dietary supplementation with Ecklonia cava. These results show that Eklonia cava exerts an anti-diabetic effect by improving blood glucose concentrations, LDL/HDL-cholesterol ratios, and antioxidative effects on the kidney in diabetic rats.

• IMMUNE NETWORK
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• 제23권5호
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• pp.40.1-40.14
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• 2023

Glucocorticoids suppress the vascular inflammation that occurs under hypercholesterolemia, as demonstrated in an animal model fed a high-cholesterol diet. However, the molecular mechanisms underlying these beneficial effects remain poorly understood. Because cholesterol is oxidized to form cholesterol oxides (oxysterols) that are capable of inducing inflammation, we investigated whether glucocorticoids affect the immune responses evoked by 7α-hydroxycholesterol (7αOHChol). The treatment of human THP-1 monocytic cells with dexamethasone (Dex) and prednisolone (Pdn) downregulated the expression of pattern recognition receptors (PRRs), such as TLR6 and CD14, and diminished 7αOHChol-enhanced response to FSL-1, a TLR2/6 ligand, and lipopolysaccharide, which interacts with CD14 to initiate immune responses, as determined by the reduced secretion of IL-23 and CCL2, respectively. Glucocorticoids weakened the 7αOHChol-induced production of CCL2 and CCR5 ligands, which was accompanied by decreased migration of monocytic cells and CCR5-expressing Jurkat T cells. Treatment with Dex or Pdn also reduced the phosphorylation of the Akt-1 Src, ERK1/2, and p65 subunits. These results indicate that both Dex and Pdn impair the expression of PRRs and their downstream products, chemokine production, and phosphorylation of signaling molecules. Collectively, glucocorticoids suppress the innate immune response and activation of monocytic cells to an inflammatory phenotype enhanced or induced by 7αOHChol, which may contribute to the anti-inflammatory effects in hypercholesterolemic conditions.

• BMB Reports
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• 제45권3호
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• pp.141-146
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• 2012

Protein tyrosine phosphatase 1B (PTP1B) is important in the regulation of metabolic diseases and has emerged as a promising signaling target. Previously, we reported the PTP1B inhibitory activity of Rheum undulatum (RU). In the present study, we investigated the metabolic regulatory effects of RU in a high-fat diet (HFD) model. RU treatment significantly blocked body weight gain, which was accompanied by a reduction of feed efficiency. In addition, it led to a reduction of liver weight mediated by overexpression of PPAR${\alpha}$ and CPT1 in the liver, and an increase in the expression of adiponectin, aP2, and UCP3 in adipose tissue responsible for the reduction of total and LDL-cholesterol levels. Chrysophanol and physcion from RU significantly inhibited PTP1B activity and strongly enhanced insulin sensitivity. Altogether, our findings strongly suggest that 2 compounds are novel PTP1B inhibitors and might be considered as anti-obesity agents that are effective for suppressing body weight gain and improving lipid homeostasis.

• 한국식품영양학회지
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• 제27권4호
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• pp.641-649
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• 2014

The antiobesity effect of commercial kochujang and fermented wheat grains in Sprague-Dawley (SD) rats was studied. The experiment was consisted of 6 groups. Normal, high fat diet (HFD), HFD+raw wheat grains, HFD+first fermented wheat grains (FFWG, with Aspergillus oryzae) HFD+final fermented wheat grains (FiFWG, fermented more for 30~40 days), and HFD+ commercial kochujang. The results showed that final body weight, weight gain, food efficiency ratio, and adipose tissue weight were markedly decreased by the commercial kochujang and the fermented wheat grains, whereas non-fermented raw wheat grains had no such effect. Lipid contents such as total lipid, total triglyceride and total cholesterol decreased in the serum and organs of liver and adipose tissues by the commercial kochujang and the fermented wheat grains as well. These results also indicated that fermented wheat grains exhibited more suppressive effects on high fat induced-obesity than raw wheat grains. Increased fermentation time and adding the red pepper powder resulted in increased the anti-obesity effect. Especially, commercial kochujang showed higher antiobestic effects than fermented wheat grains. These in vivo findings suggested that well-fermented end products of the wheat grains and red pepper powder in kochujang could be useful in the prevention of obesity.

• 한국버섯학회지
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• 제15권3호
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• pp.99-103
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• 2017

The objective of this study was to determine the anti-diabetic effect of the water extract of Neolentinus lepideus in a diabetic mouse model. Seven-week-old C57BL/KsJ-db/db mice were fed either a control diet (CD) or diet supplemented with 1% or 5% of N. lepideus water extract (NLWE1 or NLWE5) for 10 weeks. Oral administration of NLWE significantly decreased the body weight gain compared to that of CD-fed group. Mice in the NLWE group had significantly lower levels of fasting serum glucose, fatty acids, and low-density lipoprotein cholesterol compared to those in the control group. These effects were accompanied by reduced fatty liver and improved glucose tolerance in the NLWE group. Taken together, these results suggest that N. lepideus might have potential as a dietary supplement to control diabetes.

• Food Science and Biotechnology
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• 제16권1호
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• pp.43-48
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• 2007

Collagen-induced arthritis (CIA) is a model for some types of human autoimmune rheumatoid arthritis (RA). In this study, we examined whether ethanol extract of potato (Solanum tuberosum) is efficacious against CIA in mice. Potato extracts (100 and 200 mg/kg) were orally administered to DBA/1J mice once daily for 49 day after initial immunization with type II collagen. Clinical assessment of disease and measurement of paw edema were conducted throughout the study. The production of CIA-related rheumatoid factor, anti-type II collagen antibody, and cytokines were examined in DBA/1J mice. Serum levels of AST, ALT, creatinine, and lipids were measured, and antioxidant enzyme activity in the spleen was also determined. The arthritis score and paw edema were markedly suppressed in the groups treated with potato extract. Levels of rheumatoid factor, anti-type II collagen antibody, interleukin (IL)-1, IL-6, LDL-cholesterol, and malondialdehyde in sera were also reduced by potato extract treatment. The activities of glutathione peroxidase and glutathione reductase were increased in the spleens of CIA mice treated with potato extract. These findings suggest that potato extract has suppressive effects on type II collagen-induced arthritis, an animal model for human RA.

• KSBB Journal
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• 제26권2호
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• pp.126-130
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• 2011

To investigate the antihyperglycemic and antihyperlipemic effect of 80% ethanol extract of Schizandra Chinensis fruit, we induced diabetes in the rats with streptozotocin (STZ) and administered schizandra extract or Acarbose to diabetic rats for 21 days by oral administration. Consequently, the groups treated using schizandra extract decreased blood glucose levels more 39% than no treatment group and the case of Acarbose group was decreased it about 21%. The concentration of cholesterol, triglyceride and LDL-C in blood was also decreased while treating schizandra extract, on the other hand, HDL-C concentration was significantly increased it about 26%. Those results induced that anti-atherogenic index (AAI) in blood was improved more than 82% level like normal condition, especially in treatment of schizandra extract 100 mg. The lipid profiled in feces was likewise showed apparent tendency to decrease and food efficiency ratio of diabetic rats was became higher for treatment with schizandra extract, but Acarbose group had low efficiency in compared with the result of glucose level and lipid profile in blood. As a result, schizandra extract is regard a good medicine for diabetes due to improve physical constitution, blood glucose and lipid level caused hyperglycemia and suggest that schizandra extract has real effects on the diabetes complication as atherosclerosis, coronary heart disease, high blood pressure.

• 동의생리병리학회지
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• 제33권5호
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• pp.282-287
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• 2019

In this study, we investigated the anti-obesity effects of various mixtures of Atractylodes macrocephala (AM) and Amomum villosum (AV) water extracts on high-fat diet (HFD) induced mouse model. We classified five groups as follows; control, HFD, HFD + AM extracts : AV extracts (100mg/kg) (1:1), HFD + AM extracts : AV extracts (100mg/kg) (2:1), HFD + AM extracts : AV extracts (100mg/kg) (3:1). Oral administration of various mixtures of AM and AV extracts for 6 weeks inhibited HFD-induced increases of body, liver and epididymal fat weights. Also, lipid profiles including LDL cholesterol were improved by various mixtures of AM and AV extracts treatment compared with HFD-fed group. Lipogenesis-related genes such as acetyl coA carboxylase (ACC) and fatty acid synthase (FAS) in liver changed in a favorable way for lipid biosynthesis by HFD compared to control, but various mixtures of AM and AV extracts-treated groups did not. Our results show that various mixtures of AM and AV extracts can prevent HFD-induced obesity in mice and suggests that the mechanisms are involved in expressions and modifications of lipogenesis-related genes such as ACC and FAS in liver.

• 한국식품과학회지
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• 제44권3호
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• pp.324-330
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• 2012

김치유산균인 L. plantarum CIB 001(KCTC 11717 bp)을 고콜레스테롤을 식이를 섭취시킨 흰쥐에게 급여하여 혈청 지질 성상 및 간 조직의 변화를 살펴보았다. 4주간의 실험식이로 고콜레스테롤 유도한 후, 6주간 고콜레스테롤 식이와 함께 CIB 001 유산균을 $5.0-7.5{\times}10^9$ CFU/day 수준으로 급여한 결과 총 콜레스테롤을 HCD군에 비해 약 30% 정도 유의하게 감소시켰고 중성지방을 약 32% 정도 유의하게 감소시켰다. HDL-콜레스테롤에 있어서는 HCD군에 비해 CIB 001를 급여한 군(HCDL)이 약 40% 유의적으로 증가하였고, LDL-콜레스테롤은 감소하는 경향을 보였으나 표준편차가 커서 유의성이 없었다. 동맥경화지수(AI)와 심장위험인자(CRF)를 비교하였을 때, HCD군에 비하여 HCDL군에서 AI가 약 6배 유의하게 낮아졌고 CRF도 약 1.9배 유의적으로 낮아졌다. 또한, 간 조직을 관찰한 결과, 고콜레스테롤 식이에 의해 유발된 지방 변성과 손상의 경우 HCDL군에서 중심정맥을 중심으로 정상 모양의 재생과 지방 변성을 감소시키는 것이 관찰되었다. 따라서 CIB 001의 투여가 콜레스테롤 과잉혈증 상태에서 높아진 혈장 지질을 저하시키고 간 기능을 개선시켰으며 동맥경화와 심장질환의 가능성을 감소시키는 것으로 나타냈다.