• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.64-68
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• 1994

The present experiments were conducted to investigate some of the factors affecting the number of haploids derived from anther culture of Nicotiana tabacum. Anther - donor plants grown under controlled environment room at 3$0^{\circ}C$ yielded more haploid than room at 18, 25 and 26-22-18$^{\circ}C$ in anther culture. Donor plants starved of fertilizer yielded more haploids as compared to those of the well fed with fertilizer in anther culture. Pretreatment of exercised flower bud at 5$^{\circ}C$ was shown to be more effective in anther culture than pretreatment at 7 and 1$0^{\circ}C$, and the optimum temperature and period of pretreatment were 4 or 6 days at 5$^{\circ}C$.

• Journal of Life Science
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• v.11 no.4
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• pp.340-345
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• 2001

Effects of growth regulators(2,4-D, IAA, NAA, BA and kinetin) and chilling treatment on callus induction. embryogenesis and regeneration through anther cultures of B. falcatum L. were examined. Frequency of callus induction and embryogenesis was effectively increased by the treatment of chilling at 5$^{\circ}C$ for 10 days before anther inoculation. Optimal level of growth regulator for callus induction and embryogenesis from anther was 2,4-D 1.0 mg/L in Murashige and Skoog(MS) basal medium supplemented with 30 g/L sucrose, 8 g/L agar. Frequency of embryogenesis from anther derived callus was increased up to 48% or 45% by addition of IAA 0.1+ kinetin 1.0 mg/L of IAA 0.1+ BA 1.0 mg/L in MS medium, respectively, Optimal medium for obtaining green callus was MS basal supplemented with BA 1.0mg/L. Addition of auxins(IAA or NAA) inhibited the formation of green callus from anther derived callus.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.436-442
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• 1987

The variations of the anther and pistil length of some rice cultivars at the different planting density and fertility levels were tested and their inheritance mode was studied. The anther length of a spikelet on a secondary panicle branch was longer than the one of a spikelet on a primary panicle branch. In the cultivar Z97B, both the anther and the pistil length were increased slightly along the increased plant-ing spacings, But, in the cultivar IR43 no general tendency was observed. The fertilizer levels applied n the field did not affect both the anther and pistil length of a given cultivar. In a cross of IR56/IR8, the anther length showed continuous variation with longer anther being recessive in the F2 population. While, the pistil length showed a continuous variation with longer pistil being over-dominance. In all of the crosses which IR56 involved, the general and specific combining abilities were high for both the anther and pistil length. The broad sense heritability for anther length was 0.46, and for pistil length was 0.88. The correlation coefficients of anther length and pistil length were 0.33 for phenotypic and 0.44 for geno-typic.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.211-214
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• 2008

Haploid system by anther culture allows the development of homozygous lines when doubled. The response of anther culture to Basta (glufosinate) resistance was investigated on transgenic plants (cv. Anjungbyeo) in order to identify inheritance of bar gene associated with Basta. Most of the regenerated transgenic plants were sterile, and only a few plants produced viable seeds ($A_1$) in the greenhouse. The bar gene was analysis by PCR in basta resistant transgenic plant ($TA_0$). The transgenic seeds ($A_1$) were significantly germinated in Basta solution compared with non-transformed seeds. As a result of anther culture, in regenerated haploid plants, segregation ratio was 1:1 in five of eight cross combinations. In diploid plants, segregation ratio was 1:1 in seven of eight cross combinations. Although there was some differences in the cross combinations, most of the combinations had 1:1 segregation ratio which supports the theory. The difference may be a result of the small sample size or the difference of anther culture response caused by genotypic difference. Hence, when many cross combinations were anther-cultured the results would support the theory.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.161-165
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• 1969

Since Guha and Maheshwari's works on the induction of haploid plant from the cultured anther of Datura innoxia an her culture has become a big topic among geneticists and breeders. Presen paper is the summarized report on the anther culture method based on the author's recent researches.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.04a
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• pp.39-40
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• 2002

• Journal of Plant Biology
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• v.39 no.1
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• pp.9-13
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• 1996

A clone, LCM1, which encodes calmodulin (CaM) was isolated and characterized from monocot lily (Lilium longiflorum Thunb.) plants. The clone is 681 bps and contains the 447 bp coding region, 8 bp leader sequence, 210 bp 3'-untraslated region, and a poly(A) tail. The coding region of 149 amino acids encodes a protein of predicted Mr 17 kD. Comparison of the LCM1 amino acid sequence with other CaMs revealed that the protein is highly conserved among various living organisms. The expression level of calmodulin gene in lily was studied by RNA blot analysis. The LCM1 mRNA was present in all tissues tested. However, a higher level of calmodulin was observed in anther and floral bud. The level of calmodulin mRNA in anther was about 10 times higher than that in anther was about 10 times higher than that in vegetative tissues. The anther preferential expression of CaM in lily is currently investigated in dicot plants.

• Plant Resources
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• v.6 no.2
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• pp.153-158
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• 2003

Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.

• Horticultural Science & Technology
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• v.17 no.1
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• pp.7-10
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• 1999

This study was carried out with the purpose of looking into the transcriptionally regulated genes related to the anther development, characterizing them, and applying their promoters to induce male-sterile plants and restore their fertility. Fifteen anther-specific clones were isolated from the anther cDNA library of Chinese cabbage through the differential screening and sequenced partially at both ends. These partial sequence data showed that cDNA clones BAN52, 84, 101, and 229 are very similar to polygalacturonase, ascorbate oxidase, $H^+-translocating$ ATPase, and pectin esterase genes respectively. However, the other clones have not been matched to any of gene sequences in data bank. In northern dot blot analysis, the transcripts of cDNA clone BAN5, 10, 33, 52, 57, 102, 103, 215, 229 appeared in the flower bud of 2.1 mm in length and their amounts were gradually increased along with the anther development. Transcription of cDNA clone BAN32, 54, 62, 84, 101 began in flower bud of 3.9 mm, which is the late stage in anther development. However, the transcription of BAN87 was very small, but its transcript was detected in all anther developmental stages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.1
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• pp.23-27
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• 1990

To elucidate the influence of nitrogen application rate on cool tolerance at the booting stage and respiratory activity of anther at the heading stage in the rice plants, the relationships among nitrogen content in the leaf blade and leaf sheath+culm at young microspore stage, cool tolerance at the booting stage and respiratory activity of anther at the heading stage were investigated for 3 rice cultivars-Yeomyungbyeo, Unbongbyeo and Milyang 23. Nitrogen content in the leaf blade at the young microspore stage was negatively correlated with respiratory rate of anther at the heading stage and fertility index, respectively. Respiratory activity of anther at the heading stage with 10ppm nitrogen application was higher than that of anther with 100ppm nitrogen application. On the Arrhenius plot of respiratory activity of anther at the heading stage, Yeomyungbyeo and Unbongbyeo showed a break at 18$^{\circ}C$ and 18.5$^{\circ}C$, respectively, with 100ppm nitrogen application, but did not show a break in respiratory activity of anther with 10ppm nitrogen application, while Milyang 23 showed a break at 20.5$^{\circ}C$ and 21$^{\circ}C$ with 10ppm and 100ppm nitrogen application, respectively. The highest correlation coefficient between fertility and respiratory rate of anther at the heading stage was shown at 20$^{\circ}C$ in the temperature range of 15$^{\circ}C$-30$^{\circ}C$.