• Title/Summary/Keyword: antagonistic

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Root-Dipping Application of Antagonistic Rhizobacteria for the Control of Phytophthora Blight of Pepper Under Field Conditions

  • Sang, Mee-Kyung;Oh, Ji-Yeon;Kim, Ki-Deok
    • The Plant Pathology Journal
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    • v.23 no.2
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    • pp.109-112
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    • 2007
  • This study was to examine the efficacy of a root-dipping application of antagonistic bacterial strains for the control of Phytophthora blight of pepper caused by P. capcisi, and to evaluate their plant growth-promoting effects in the field in 2005 and 2006. The candidate antagonistic rhizobacterial strains CCR04, CCR80, GSE09, ISE13, and ISE14 were treated by dipping plant roots with bacterial suspensions prior to transplanting. The candidate rhizobacterial strains CCR04, CCR80, GSE09, and ISE14 significantly (P=0.05) reduced the disease incidence and the area under the disease progress curves when compared to buffer-treated controls in at least a year test. The metalaxy l(fungicide-treated control) resulted in one of the lowest disease incidences among the treatments in both years. Moreover, the strains CCR04, CCR80, GSE09, and ISE13 significantly (P=0.05) increased the fruit weights and/or numbers of peppers in at least a year test compared to the buffer-treated controls. These results suggest that the antagonistic rhizobacterial strains CCR04, CCR80, and GSE09 could be efficient biocontrol agents by controlling Phytophthora blight of pepper and promoting the plant growth when treated with root-dipping at transplanting.

Molecular Differentiation of Bacillus spp. Antagonistic Against Phytopathogenic Fungi Causing Damping-off Disease

  • Cho, Min-Jeong;Kim, Young-Kwon;Ka, Jong-Ok
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.599-606
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    • 2004
  • Gram-positive antagonistic bacilli were isolated from agricultural soils for possible use in biocontrol of plant pathogenic fungi, Fusarium oxysporum, Rhizoctonia solani, and/or Pythium ultimum. Among the 65 antagonistic Gram-positive soil isolates, 22 strains were identified as Bacillus species by 16S rDNA sequence analyses. Four strains, including DF14, especially exhibited multiple antagonistic properties against the three damping-off fungi. Genotypic properties of the Bacillus isolates were characterized by rapid molecular fingerprinting methods using repetitive extragenic palindromic-PCR (REP-PCR), ribosomal intergenic spacer-length polymorphisms (RIS-LP), 16S rDNA PCR-restriction fragment length polymorphisms (PCR-RFLP), and strain-specific PCR assays. The results indicated that the REP-PCR method was more valuable than the RIS-LP and 16S rDNA PCR-RFLP analyses as a rapid and reliable approach for bacilli typing and identification. The use of strain-specific primers designed based on 16S rDNA sequence comparisons enabled it to be possible to selectively detect a strain, DF14, which is being used as a biocontrol agent against damping-off fungi.

Model Estimation and Precise Position Control of an Antagonistic Actuation with Pneumatic Artificial Muscles (공압형 인공근육을 이용한 상극 구동의 모델 추정 및 정밀 위치제어)

  • Kang, Bong-Soo
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.35 no.5
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    • pp.533-541
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    • 2011
  • This paper presents a frequency-response test performed on an antagonistic actuation system consisting of two Mckibben pneumatic artificial muscles and a pneumatic circuit with pressure valves. Varying switching frequency to pressure valves from 0.1 Hz to 5 Hz, parameters of a linear model were estimated optimally to predict dynamic characteristics of the antagonistic actuation. A model-base control scheme with estimated parameters was built for the precise trajectory tracking of the antagonistic structure and realized on a reconfigurable embedded control system, CompactRIO. Experimental results showed that the proposed model-based control scheme gave good performance in trajectory tracking comparing with a PD control scheme when square wave and sinusoidal wave were given as references to follow.

Antimicrobial test of Antagonistic Microbes for Biological Control of Large patch of Zoysiagrass (잔디 Large patch의 생물학적 방제를 위한 길항 미생물의 선발과 항균력 검정)

  • Ma, Gi-Yoon;Lee, Geung-Joo
    • Proceedings of the Turfgrass Society of Korea Conference
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    • 2011.02a
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    • pp.35-35
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    • 2011
  • A large patch disease caused by Rhizoctonia solani AG2-2(IV) is a serious problem in turfgrass sites including golf courses and sports fields in Korea. The objectives of this study were to isolate some antagonistic microorganisms and to explain some involving mechanisms. Initially single colonies which were formed from the filtrates of various soil samples were obtained from LB culture and then co-cultured with R.solani AG2-2(IV) on PDA plate to explore some antagonistic microbes against for large patch fungus, Rhizoctonia solani AG2-2(IV). Out of total 82 antagonistic isolates which commonly had inhibition effect on Rhizoctonia solani AG2-2(IV) mycelial growth, one candidate (YPIN22) showed the most antifungal effect, which was confirmed by the longest distance from the edge of bacterial colony to the mycelial edge of the Rhizoctonia solani AG2-2(IV) in the dual culture. A succeeding investigation was to test any potential effect of the isolate on growth inhibition of 5 other turfgrass pathogens including R. solani solani AG2-2(IIIB), P. ultimum, C. caudatum, C. lunata, and F.oxysporum. Preliminary result indicated that the new isolate YPIN22 was also found to have antagonistic potential on the growth inhibition of those turfgrass pathogenic fungi, which was explained by inhibition zones ranging from 8 to 22mm. A further explanation of some characteristics of the isolate YPIN22 will be discussed in detail.

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Analysis on characteristics of antagonistic stiffness of the planar 3 degrees-of-freedom RCC mechanism (평면형 3 자유도 RCC 메카니즘의 상반효과로 인한 강성 특성 분석)

  • 이병주;김희국;이준용
    • 제어로봇시스템학회:학술대회논문집
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    • 1996.10b
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    • pp.718-721
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    • 1996
  • In this work, an effective stiffness generated by internal loading for a planar 3 degrees of freedom RCC mechanism is investigated. For this purpose, the internal kinematic analysis and antagonistic stiffness modeling for this mechanism are performed. It is shown that the antagonistic stiffness could be effectively created at the center of the mechanism in its symmetric configuration.

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Bradykinin Antagonistic Activities of Antihistamine Agents Containing Piperazine Moiety (피페라진계 항히스타민제들의 브라디키닌 길항작용)

  • 정성현;이소영;윤혜숙
    • YAKHAK HOEJI
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    • v.37 no.6
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    • pp.625-630
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    • 1993
  • Among the six antihistamine agents tested in this study, homochlorcyclizine showed the highest bradykinin antagonistic activity in the receptor binding assay as well as the isolated rat ileum assay. Schild plot analysis of bradykinin-induced ileal contraction in the presence of three different concentrations of homochlorcyclizine revealed a pA$_{2}$=6.26, and a correlation coefficient of 0.984. Homochlorcyclizine of (100 $\mu{M}$ final concentration) also showed 25% antagonistic activity in the receptor binding assay.

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Isolation of Antagonostic Fungi to Phytophthora Capsici for Biological Control of Phytophthora Blight of Red-Pepper (고추역병의 생물학적 방제를 위한 길항진균의 분리)

  • 이용세;전하준;김상달
    • Korean Journal of Organic Agriculture
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    • v.6 no.2
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    • pp.117-125
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    • 1998
  • For isolation of antagonistic fungi antagonistic to Phytophthora capsici, a total of 157 isolates of fungi were screened from soil. Among the 157 isolates further screened by the dual culture test on potato dextrose agar and V-8 juice agar, 16 isolates were tested to show their antagonistic activity against P. capsici and Fusarium oxysporum. Fungal cul-ture filtrates of screened 16 isolates were shown to inhibit germination of zoospoorangia of P. capsici entirely and conidia of F. oxysporum considerably. Antagonistic fungi were shown to suppress of P. capsici infection of red-pepper plants maintained in the green house. Four isolates. 27 J5, 37 J10, 36 J13 and 31 K10, with the reduced disease incidence 53.3∼60.0% were identified as Fusarium sp. (27 J5). Trichoderma sp. (37 J10, 36 J13) and Penicillium sp. (31 K10).

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Dynamic Characteristics of an Antagonistic Actuation with Pneumatic Artificial Muscles (공압형 인공근육을 이용한 상극구동의 동적 특성)

  • Kang, Bong-Soo;Song, Seung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.33 no.10
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    • pp.1081-1086
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    • 2009
  • This paper presents dynamic characteristics of pneumatic artificial muscles. Since the actuating performance of a pneumatic muscle is closely related to the input pressure of a pneumatic muscle, the air flow model on a valve orifice and an elastic bladder of the muscle is formulated to estimate precisely the pressure variance of pneumatic muscles during deflating and inflating process. Frequency response experiments are performed with an antagonistic system consisting of two pneumatic muscles and fast pneumatic control valves. Comparing with experimental results, the proposed model yielded good performance in estimating dynamic motions of the antagonistic system as well as the pressure variance of the pneumatic artificial muscles

Multiple Simultaneous Specification Control of Antagonistic Actuation by Pneumatic Artificial Muscles (공압형 인공근육으로 구동되는 상극구동의 다중 동시 사양 제어)

  • Kang, Bong-Soo
    • The Journal of Korea Robotics Society
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    • v.6 no.1
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    • pp.34-41
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    • 2011
  • This paper presents a frequency-response test performed on an antagonistic actuation system consisting of two Mckibben pneumatic artificial muscles and a pneumatic circuit. A linear model, capable of estimating the dynamic characteristics of the antagonistic system in the operating range of pneumatic artificial muscles, was optimally calculated based on frequency-response results and applied to a multiple simultaneous specification control scheme. Trajectory tracking results showed that the presented multiple simultaneous specification controller, built experimentally by three PD typed sample controllers, satisfied successfully all required control specifications; rising time, maximum overshoot, steady-state error.

Effect of Continuous Antagonistic Muscle Strengthening and Evjenth-Hamberg Stretching on Pulmonary Function of Forward Head Posture Subjects

  • Park, Joo Hyun
    • Journal of International Academy of Physical Therapy Research
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    • v.6 no.2
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    • pp.871-877
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    • 2015
  • This research was conducted to investigate the effect of continuous antagonistic muscle strengthening exercise and Evjenth-Hamberg stretching on the pulmonary function of university students with abnormal transformation of forward head posture(FHP). The results of study subject to the continuous antagonistic muscle strengthening(CAS) group(n=10) and Evjenth- Hamberg stretching(EHS) group(n=10) that was conducted 3 times a week for 6 weeks are as follow. FVC, IVC, and MVV all were shown to be significant in the pre post comparison between the CAS group and EHS group(p<.05), and significant difference was shown for MW between the two groups(p<.05) in which the CAS group showed better effect. Based on the results above, it is considered that combining continuous antagonistic muscle strengthening exercise has better effect on pulmonary function compared to application of only Evjenth-Hamberg stretching.