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Superoxide and Nitric Oxide Involvement in Enhancing of N-methyl-D-aspartate Receptor-Mediated Central Sensitization in the Chronic Post-ischemia Pain Model

  • Ryu, Tae-Ha;Jung, Kyung-Young;Ha, Mi-Jin;Kwak, Kyung-Hwa;Lim, Dong-Gun;Hong, Jung-Gil
    • The Korean Journal of Pain
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    • v.23 no.1
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    • pp.1-10
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    • 2010
  • Background: Recent studies indicate that reactive oxygen species (ROS) are involved in persistent pain, including neuropathic and inflammatory pain. Since the data suggest that ROS are involved in central sensitization, the present study examines the levels of activated N-methyl-D-aspartate (NMDA) receptors in the dorsal horn after an exogenous supply of three antioxidants in rats with chronic post-ischemia pain (CPIP). This serves as an animal model of complex regional pain syndrome type-I induced by hindpaw ischemia/reperfusion injury. Methods: The application of tight-fitting O-rings for a period of three hours produced CPIP in male Sprague-Dawley rats. Allopurinol 4 mg/kg, allopurinol 40 mg/kg, superoxide dismutase (SOD) 4,000 U/kg, N-nitro-L-arginine methyl ester (L-NAME) 10 mg/kg and SOD 4,000 U/kg plus L-NAME 10 mg/kg were administered intraperitoneally just after O-ring application and on the first and second days after reperfusion. Mechanical allodynia was measured, and activation of the NMDA receptor subunit 1 (pNR1) of the lumbar spinal cord (L4-L6) was analyzed by the Western blot three days after reperfusion. Results: Allopurinol reduced mechanical allodynia and attenuated the enhancement of spinal pNR1 expression in CPIP rats. SOD and L-NAME also blocked spinal pNR1 in accordance with the reduced mechanical allodynia in rats with CPIP. Conclusions: The present data suggest the contribution of superoxide, produced via xanthine oxidase, and the participation of superoxide and nitric oxide as a precursor of peroxynitrite in NMDA mediated central sensitization. Finally, the findings support a therapeutic potential for the manipulation of superoxide and nitric oxide in ischemia/reperfusion related pain conditions.

New Record of the Spinecheek Gudgeon, Eleotris acanthopoma (Perciformes: Eleotridae) from Jeju Island, Korea (제주도 남부 연안역에서 채집된 농어목 구굴무치과 한국미기록종, Eleotris acanthopoma)

  • Kim, Byung-Jik;Kweon, Seon-Man;Cho, Hyun-Geun;Choi, Seung-Ho;Kang, Dong-Won
    • Korean Journal of Ichthyology
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    • v.27 no.1
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    • pp.50-54
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    • 2015
  • Based on four specimens (50.9~69.0 mm SL) collected from a small stream of southern coast of Jeju Island, Eleotris acanthopoma was described as the second Eleotris species as well as the first record from Korea. They were characterized by having two dorsal fins, well-separated pelvic fins, separation of two rows of sensory papillae on opercle and extending the fourth row of sensory papillae beyond the 13th longitudinal sensory-papillae row on cheek. We proposed a new Korean name, "Geom-eun-gu-gul-mu-chi", for the species.

A Study on the Costume Culture of Xiongnu (흉노(匈奴)의 복식문화에 관한 연구)

  • Kim, Yong-Mun
    • Journal of the Korean Society of Costume
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    • v.63 no.3
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    • pp.1-16
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    • 2013
  • Xiognu people were the first of the Central-Asian nomads to establish a nation in 209 B.C. They always moved around looking for places to breed their animals and fertile grounds, so they wore clothes made of fur and leather and covered their tents with felt from the livestock. This research studies on the literatures, costumes and the achievement of archaeological excavation. Furthermore, to investigate on costumes excavated of Xiongnu, we visited the Mongolian National Museum and the Hermitage Museum. A corn-hat made of felt, a felt hat with ear flaps and a golden crown with a bird on the top were unearthed from a tomb of Xiongnu in Inner mongolia. Women usually wore pigtails, and men wore pigtails or ponytails but they cut their hair short when holding a funeral. Many pigtails discovered in Noyon uul tombs can be considered as their funeral customs. The Xiongnu wore a round or v-neck caftan attached straight sleeves reaching knees in the left folded style, and because they always rode horses, having the length of the caftan not go past their buttocks would have made it more convenient for them. During the period of Western Han, Ho refered to Xiongnu and it became a common name for northern races. They used leather belts and an animal-designed buckle was found. Women commonly rouged their cheeks for a vivid and cute look, and many ornaments were excavated including bracelets, rings and decorations made of gold, silver, copper and jade, among which there were hair ornaments used to identify one's class. A horse pattern with wings and a horn of Golmod T20 was substitution for the Schythian use of deer. Patterns or shape of unearthed articles present in the Xiongnu culture in Noyon uul had a close relationship with Altaic, Greek and Persian cultures. The Xiongnu clothing was made of animals' skin and fur, woolen textiles and felt. It was folded to the left for upper garments, and the pants were adjusted using a belt and shoes were made of leather, which was very suitable for protection against the cold and horse riding. Mobility played a significant role in their clothing.

Evaluation of Meals Students Consumed in College Foodservice (일부 서울지역 대학식당의 메뉴분석)

  • Song, Yoon-Ju;Park, Jung-Sook;Paik, Hee-Young;Lee, Yeon-Sook
    • Journal of the Korean Society of Food Culture
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    • v.14 no.1
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    • pp.1-15
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    • 1999
  • A survey was conducted in 591 college students using 8 different cafeterias serving 31 meals. The information was obtained about the name and amount of dishes they consumed from the menu offered by college foodservice. Mean nutrient contents per meal in offered menu were higher than 1/3 of RDA for their age, sex in offered menu. The mean energy content was 466kcal from rice, 113kcal from soup, 141kcal from side dish and 21kcal from kimchi. When side dishes were classified by cooking methods, side dishes using grilling, frying, roasting methods had high energy content and fat percent above 200kcal, 40% per dish. By main ingredients, side dishes with animal food were higher energy than with plant food. Students consumed 94% of the energy provided in offered menu. When compared to proportion of foods consumed by sex, there was 43% of female and 22% of male consumed less than half in soup and 31% of female and 12% of male in kimchi. The most prevalent menu patterns of subjects included rice, soup, 2 side dishes, kimchi and were the same in both sexes. Mean energy intakes per meal were 989kcal for males and 842kcal for females which were enough to meet 1/3 of RDA for their age and sex. Most nutrient intake except fat and vitamin B1 were higher in set menu than in cafeteria. Nutrient adequacy ratio(NAR) were above 0.9 except calcium and vitamin A. The mean energy intake was 542kcal from rice, 70kcal from soup, 164kcal from side dish and 20kcal from kimchi. In conclusion, intake of most of the nutrients of students obtained from males in college foodservice were sufficient but calcium intake was insufficient and fat content was above 20% of energy. High fat consumption was due to side dishes from frying, grilling, roasting with animal food. To provide desirable meals in college foodservice, use of fat in cooking must be decreased.

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Quantitative Analysis of Microperfusion in Contrast-Induced Nephropathy Using Contrast-Enhanced Ultrasound: An Animal Study

  • Nieun Seo;Hyewon Oh;Hyung Jung Oh;Yong Eun Chung
    • Korean Journal of Radiology
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    • v.22 no.5
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    • pp.801-810
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    • 2021
  • Objective: To investigate imaging biomarkers of microperfusion in contrast-induced nephropathy (CIN) using contrast-enhanced ultrasound (CEUS). Materials and Methods: The CIN model was fabricated by administering indomethacin (10 mg/kg), L-NAME (15 mg/kg), and iopamidol (10 mL/kg) to Sprague-Dawley rats. After 24 hours, CEUS was performed on CIN (n = 6) and control (n = 6) rats with sulphur hexafluoride microbubbles (SonoVue). From time-intensity curves obtained from the kidney arriving time (AT), acceleration time (AC), time to peak (TTP), and peak enhancement (PE) were measured and compared between the groups. After CEUS, the rats were sacrificed, and cell apoptosis markers were evaluated to confirm the development of CIN. Results: Among CEUS parameters, AT (7.8 ± 1.6 vs. 4.2 ± 0.5 s, p = 0.002), AC (4.7 ± 1.4 vs. 2.0 ± 0.4 s, p = 0.002), and TTP (12.5 ± 2.9 vs. 6.2 ± 0.6 s, p = 0.002) were significantly prolonged in the CIN group compared to controls. PE was significantly higher in the control group than in the CIN group (17.1 ± 1.9 vs. 12.2 ± 2.0 dB, p = 0.004). In kidney tissue, mRNA and protein levels of the apoptotic makers were significantly higher in the CIN group than in the control group (p = 0.003 and p = 0.002). Conclusion: CEUS parameters can be used as imaging biomarkers for microperfusion in CIN. In rats with CIN, AT, AC, and TTP were significantly prolonged, while PE was significantly lower compared to controls.

Practical Propagation Methods for Production of Prothalli and Sporophytes in Deparia pycnosora (Christ) M. Kato

  • Jang, Bo Kook;Park, Kyungtae;Cho, Ju Sung;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.43-43
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    • 2019
  • Deparia pycnosora (Christ) M. Kato is a fern used as ornamental plant. In addition, it is called "Teol-go-sa-ri" in Korean name. The aim of this study was to develop a practical propagation method of D. pycnosora using tissue culture technique. Prothallus obtained from spore germination was the used as experiment materials. The prothalli (300 mg) used in all experiments were sub-cultured for 8-week intervals. The most suitable media for prothallus propagation were identified by culturing 300 mg of prothalli in $1/4{\times}$, $1/2{\times}$, $1{\times}$, $2{\times}$ MS medium and in Knop medium for 8 weeks. Also, the prothalli were cultured by chopping with a scalpel. In addition, sucrose, activated charcoal, and total nitrogen source were added in different concentrations based on the culture medium selected. Cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $30{\times}1.0{\mu}mol-m-2{\cdot}s-1$, and a photoperiod of 16/8 h (light/dark) in in vitro. The results showed that optimum was achieved prothallus fresh weight and development in $1{\times}$ MS medium. When other components were added to the basic $1{\times}$ MS medium, prothallus propagation was maximized in $1{\times}$ MS medium supplemented with 2% sucrose, 0.2% activated charcoal, and 60 mM total nitrogen. To select a suitable soil mixture for sporophyte formation, 1.0 g of prothallus was blended with distilled water, spread on five combinations of different soil substrates (decomposed granite, horticultural substrates, peat moss, and perlite), and cultivated for 12 weeks. The sporophyte cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $43{\pm}2.0{\mu}mol-m-2{\cdot}s-1$, humidity of $84{\pm}1.4%$, and a photoperiod of 16/8 h (light/dark). As a results, horticultural substrate alone, 2:1 (v:v) mixtures of horticultural substrate and perlite, and 2:1 mixtures of horticultural substrate and decomposed granite induced 208.0, 201.3 and 248.8 sporophytes per pot, respectively. Therefore, this result could provide a practical mass propagation method of D. pycnosora

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Effect of Mecadox in Treatment of Colibacillosis and Prevention of Experimental Salmonella Infection in Swine (자돈하리증(仔豚下痢症) 및 Salmonella 인공감염증(人工感染症)에 대한 Mecadox의 치료 및 예방효과)

  • Seo, Ik Soo;Chung, Gill Taik;Han, In Kyu
    • Korean Journal of Veterinary Research
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    • v.15 no.1
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    • pp.119-121
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    • 1975
  • The effect of mecadox, trade name of carbodox, in the treatment of colibacillosis and in the prevention of experimental Salmonella infection in swine was studied throughout the experimental period of four weeks using a total of 70 piglets. The results obtained are as followings. 1. Mecadox was found moderately effective as a therapeutic for colibacillosis of piglets when administered orally as pig doser or added to the feed at the ratio of 50 ppm with or without the addition of vitamin A and $D_3$. 2. In the prevention of experimental infection with Sal. choleraesuis, mecadox was found highly effective. As judged by clinical symptoms such as diarrhea and fever together with the confirmation of excretion of the causative organisms in the feces, the best result was obtained with mecadox pig doser followed by mecadox (50ppm) with vitamin A and $D_3$, and mecadox (50 ppm) without the vitamins in the descending order of effectiveness.

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New Record of Sillago sinica (Pisces: Sillaginidae) in Korean Waters, and Re-identification of Sillago parvisquamis Previously Reported from Korea as S. sinica

  • Bae, Seung Eun;Kwun, Hyuck Joon;Kim, Jin-Koo;Kweon, Seon-Man;Kang, Chung Bae
    • Animal Systematics, Evolution and Diversity
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    • v.29 no.4
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    • pp.288-293
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    • 2013
  • A single specimen of the genus Sillago, collected from Gwangyang, Korea, in May 2009, is characterized by XI first dorsal fin spines, 3 or 4 rows of melanophore pattern along the second dorsal fin membrane, and a darkish posterior margin of the caudal fin. Our specimen was identified as Sillago sinica reported as a new species; this identification is confirmed by mitochondrial DNA cytochrome oxidase subunit I sequences, which show that our specimen corresponds to S. sinica (d=0.000) and differs from the congeneric species Sillago parvisquamis (d=0.170). Comparisons of Korean specimens previously reported as S. parvisquamis with specimens of S. sinica show that the S. parvisquamis specimens are actually S. sinica. We propose the new Korean name "buk-bang-jeom-bo-ri-myeol" for S. sinica.

First Record of the Gobiid fish, Clariger chionomaculatus (Perciformes: Gobiidae) from off Geojedo Island, Korea (우리나라 거제도 연안에서 채집된 망둑어과 첫기록종, Clariger chionomaculatus)

  • Cho, Hyun-Geun;Kim, Byung-Jik
    • Korean Journal of Ichthyology
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    • v.32 no.2
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    • pp.110-114
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    • 2020
  • A single specimen (40.5 mm in SL) of Clariger chionomaculatus was collected at 4 m depth on mud bottom from off Geojedo Island, Korea. This species is characterized by having an elongated body without scales, several barbels below eyes, I, 12~15 second dorsal fin rays, I, 12~14 anal fin rays; 19~20 pectoral fin rays with one free soft ray on upper part, and a unique color pattern with numerous white blotches on dorsal part of body. Because it has been known from Japan only to date, we describe in detail C. chionomaculatus as the first record from Korea. A new Korean name, "Huin-jeom-wae-mang-dug", is proposed for the species.

New Record of the Gobiid Fish, Parioglossus dotui(Pisces, Gobiidae) from Korea (한국산 망둑어과 어종 1미기록종, Parioglossus dotul)

  • Kim Yong Uk;Han, Kyeong Ho
    • Animal Systematics, Evolution and Diversity
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    • v.9 no.1
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    • pp.51-59
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    • 1993
  • A gobiid fish, Parioglossus dotui Tomiyama is described from 359 specimens collected at Cheju-do and Pusan, in August 1994 of 1986. This is the first definitive record of the species in the Korean waters. Parioglossus dotui having a small-sized body usually is found swimming in schools. The branchiostegals are 5 in number on each side. It grows up to 40mm in total length. Most of ventral fins are separated and connected with rudimentray membrane. In this species, the lateral line were indistincet or absent. The caudal fin slightly emarginates or truncates in both sexes, with a dark spot at the border of the base. It Inhabits inlet waters and estuaries of rivers. "Deungsul mang-dug" is proposed as Korean name of this species.

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