• 한국임상수의학회지
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• 제23권2호
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• pp.180-182
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• 2006

아일랜드 고르트주에서 사육중인 5살, 수컷, 아랍종말이 결막염으로 진단되었다. BL01, BL02 및 ST01에 자침 및 덱사메타손 수침(2회/주)을 적용하였고, 동시에 상초 영역에 안침을 실시하였다. 본 환축은 3회 침 치료 후, 눈물, 안 분비물 및 충혈이 소실되었다. 본 환축은 침 치료에 양호한 치료반응을 보인 말 결막염의 증례이었다.

• 한국발생생물학회지:발생과생식
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• 제20권3호
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• pp.219-225
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• 2016

Most of the commercial devices for vitrification are directly immersed into the warming solution (WS) for increasing of warming rate. However, the previous modified cut standard straw (MCS) which has reported is difficult to immerse into the WS. The aim of this study was to investigate whether the long cut straw (LCS) could be useful as a stable tool for vitrified-warmed human blastocysts. A total of 138 vitrified-warmed cycles were performed between November 2013 and November 2014 (exclusion criteria: women ${\geq}38$ years old, poor responder, surgical retrieval sperm, and severe male factor). The artificial shrinkage was conducted using 29-gauge needles. Ethylene glycol and dimethyl sulfoxide (7.5% and 15% (v/v)) were used as cryoprotectants. Freezing and warming were conducted using the LCS tool. The cap of LCS was removed using the forceps in the liquid nitrogen ($LN_2$) and then directly immersed into the first WS for 1 min at $37^{\circ}C$ (1 M sucrose). Only re-expanded blastocysts were transferred after it was cultured in sequential media for 18-20 h. A total of 294 blastocysts were warmed, and all were recovered (100%). Two hundred eighty-five embryos were survived (96.9%). The vitrified-warmed blastocysts of all patients were transferred without any cancellation. We were able to achieve a reasonable implantation (24.2%), following by clinical pregnancy (36.2%), which then continued to ongoing pregnancy (36.2%), and live birth (31.2%). Using LCS is achieved the acceptable rates of survival, pregnancy and live birth. Therefore, the LCS could be considered as a stable and simple tool for human embryo vitrificaton.

• 한국임상수의학회지
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• 제27권6호
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• pp.735-739
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• 2010

8개월령 말티스가 호흡곤란, 운동불내성의 증상으로 내원하였다. 흉부방사선상 환자는 심한심종대와 주폐동맥의 확장 소견을 보였고, 심초음파상 대동맥근이 우심실을 향하고 좌-우 단락을 가지고 있는 대동맥하 심실중격결손증이 확인되었다. 또한 중격결손에 의한 좌-우 단락성 혈류에 의해 폐혈관계에 과순환 소견과 폐성 고혈압에 의한 심한 폐동맥 역류가 (최고속도 4.7 m/s, 압력구배 ~88 mmHg) 확인되었다. 상기의 소견을 토대로 환자는 양대혈관 우심실기시로 진단되었다. 환자는 우심실의 혈량과부하를 줄이기 위한 이뇨제 처치(furosemide 1 mg/kg), 심장재구성을 늦추어주기 위해 spironolatcone (1 mg/kg) 및 enalapril (0.5 mg/kg) 처치와 폐의 과순환과 폐성 고혈압을 완화시켜주기 위해 sildenafil (1 mg/kg)를 처방하였다. 일주일뒤 재검에서 환자의 임상 증상은 크게 개선되었다. 현재 환자는 생존해 있고 정기적으로 모니터하고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.567-572
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• 2010

The objective was to assess the concentrations of Vitamins A and E in blood of growing lambs infected or not with gastrointestinal nematodes (GIN) and fed a diet containing clinoptilolite. Twenty-four male lambs were used. A $2{\times}2$ factorial design consisting of two feeding treatments (B and Z) and two levels of parasitic status, infected (I) and uninfected (U) was used. Lambs were randomly assigned to one of four (n = 6), groups: BU (basal-uninfected), BI (basal-infected), ZU (zeolite-uninfected) and ZI (zeoliteinfected). Lambs of groups BI and ZI were infected with a single dose of 15,000 $L_3$ larvae of GIN. Blood samples were collected from individual animals at the start of the experiment and, thereafter, at 15-day intervals. The average blood serum vitamin A and vitamin E, concentration in lambs (mean${\pm}$SD) was 0.25${\pm}$0.090 ${\mu}g/ml$ and 1.59${\pm}$0.769 ${\mu}g/ml$, respectively. Lambs fed Z diet had higher values of vitamin A (p<0.001), but lower values of vitamin E (p<0.01) when compared with those fed B diet.

• 대한수의사회지
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• 제13권3호
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• pp.167-168
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• 1977

A case of canine babesiasis in a about 2 years old male Retriever dog was presented. The diagnosis was comfirmed by the examination of blood smear with Giemsa stain. The patient was completly recovered with the intramuscular injection of $7{\%}$

• 대한수의사회지
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• 제34권11호
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• pp.786-787
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• 1998

수의사가 애완동물 소유자에게 적합한 치료를 함으로써 동물병원을 성공적으로 경영 한다. 적합한 치료로 인해 꾸준히 애완동물 소유자가 동물병원을 방문하게 된다. 따라서, 동물병원은 더 많은 수입을 올릴 수 있다. 동물병원의 올바른 백신 접종안(프로그램)은 동물병원과 고객사이에 강한 유대관계를 유지하게 하는 초석이 된다.

• 한국임상수의학회지
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• 제24권2호
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• pp.214-217
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• 2007

약 2개월령(個月齡)의 잡종 암컷 고양이가 영창동물병원(永昌動物病院)에 내원하였다. 1차(次) 진료시(診療時) 우측 눈의 축동(縮瞳) 및 순막 돌출(瞬膜 突出)이 인정되었으며, 또한 좌측 눈의 경도의 축동이 인정되어, 본 환축(患畜)은 고양이 Horner's syndrome으로 진단(診斷)되었다. 본 환축(患畜)에 대하여 안침(眼針) (상초 영역(上焦 領域), 10분간(分間)) 및 dexamethasone 약침(藥鍼)(0.2 ml/혈위: BL 01-정명(睛明) BL02-찬죽(纘竹) 및 ST01-승읍(承泣))을 각각(各各) 실시(實施)하였다. 1차 치료 후, 축동(縮瞳) 및 순막돌출(瞬膜突出)이 놀랍게도 소실되었다. 2차 진료시(1차 치료 후 1일)확인 결과, 눈의 소견은 정상 상태를 유지하고 있었다. 따라서 본(本) 증례(症例)는 침치료(鍼治療)에 양호(良好)한 반응(反應)을 나타낸 고양이 Horner's syndrome의 증례(症例)였다.

• Animal Bioscience
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• 제37권3호
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• pp.522-535
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• 2024

Objective: Transition period is considered from 3 weeks prepartum to 3 weeks postpartum, characterized with dramatic events (endocrine, metabolic, and physiological) leading to occurrence of production diseases (negative energy balance/ketosis, milk fever etc). The objectives of our study were to analyze the periodic concentration of serum beta-hydroxy butyric acid (BHBA), glucose and oxidative markers along with identification, and validation of the putative markers of negative energy balance in buffaloes using in-silico and quantitative real time-polymerase chain reaction (qRT-PCR) assay. Methods: Out of 20 potential markers of ketosis identified by in-silico analysis, two were selected and analyzed by qRT-PCR technique (upregulated; acetyl serotonin o-methyl transferase like and down regulated; guanylate cyclase activator 1B). Additional two sets of genes (carnitine palmotyl transferase A; upregulated and Insulin growth factor; downregulated) that have a role of hepatic fatty acid oxidation to maintain energy demands via gluconeogenesis were also validated. Extracted cDNA (complementary deoxyribonucleic acid) from the blood of the buffaloes were used for validation of selected genes via qRTPCR. Concentrations of BHBA, glucose and oxidative stress markers were identified with their respective optimized protocols. Results: The analysis of qRT-PCR gave similar trends as shown by in-silico analysis throughout the transition period. Significant changes (p<0.05) in the levels of BHBA, glucose and oxidative stress markers throughout this period were observed. This study provides validation from in-silico and qRT-PCR assays for potential markers to be used for earliest diagnosis of negative energy balance in buffaloes. Conclusion: Apart from conventional diagnostic methods, this study improves the understanding of putative biomarkers at the molecular level which helps to unfold their role in normal immune function, fat synthesis/metabolism and oxidative stress pathways. Therefore, provides an opportunity to discover more accurate and sensitive diagnostic aids.

• Biomolecules & Therapeutics
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• 제24권3호
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• pp.305-311
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• 2016

Mitochondria-targeted vitamin E (MVE) is designed to accumulate within mitochondria and is applied to decrease mitochondrial oxidative damage. However, the protective effects of MVE in skin cells have not been identified. We investigated the protective effect of MVE against UVB in dermal fibroblasts and immortalized human keratinocyte cell line (HaCaT). In addition, we studied the wound-healing effect of MVE in animal models. We found that MVE increased the proliferation and survival of fibroblasts at low concentration (i.e., nM ranges). In addition, MVE increased collagen production and downregulated matrix metalloproteinase1. MVE also increased the proliferation and survival of HaCaT cells. UVB increased reactive oxygen species (ROS) production in fibroblasts and HaCaT cells, while MVE decreased ROS production at low concentration. In an animal experiment, MVE accelerated wound healing from laser-induced skin damage. These results collectively suggest that low dose MVE protects skin from UVB irradiation. Therefore, MVE can be developed as a cosmetic raw material.

• Parasites, Hosts and Diseases
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• 제56권4호
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• pp.371-374
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• 2018

A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were examined in a diagnostic laboratory, using multiplex real-time polymerase chain reaction (PCR) against 23 diarrheal pathogens. Sequence analysis was performed using nested PCR amplicon of 18S ribosomal RNA. Coccidian oocysts were identified in the fecal smear. Although multiplex real-time PCR was positive for Cyclospora cayetanensis, the final diagnosis was Cystoisospora ohioensis infection, confirmed by phylogenetic analysis of 18S rRNA. To our knowledge, this the first case report of C. ohioensis in Korea, using microscopic examination and phylogenetic analysis.