• 한국산학기술학회논문지
• /
• 제17권9호
• /
• pp.126-132
• /
• 2016

본 연구는 전산유체역학 기법을 이용하여 수소 생산 플랜트의 개질 튜브 공정가스와 버너 가스 온도에 따른 화학반응과 열변형 특성을 분석한다. 개질로 내부의 온도는 약 800 K 내지 1000 K 이상으로 고온으로 유지되기 때문에 튜브의 열변형 문제가 심각하게 발생할 수 있다. 따라서 개질로의 구조건전성을 평가하고 안정된 생산력을 가진 장비를 운영하기 위해서 반응과 열변형 특성에 대한 이해는 필수적이다. 본 연구는 상용 전산해석 코드(ANSYS Fluent/Mechanical V.13.0)를 사용하여, 대류, 전도 및 복사 열전달을 포함한 복합 열전달과 난류유동을 3차원적으로 해석하였다. 특히, 열유동 특성에 따른 연성해석(Fluid-Solid Interaction: FSI)를 수행하였으며 고온 버너가스와 공정가스 운전조건에 따른 반응 특성과 열변형 변화를 분석하였다. 수치해석 결과, 개질 공정가스와 버너 가스의 주입온도가 각각 200 K 감소하면, 수소생성량은 최대 약 4 배, 최소 약 2 배 감소한다. 또한, 공정가스와 버너 가스의 주입온도에 따라 열변형은 최대 약 20%, 최소 약 15% 감소한다.

• Journal of Plant Biotechnology
• /
• 제36권4호
• /
• pp.384-391
• /
• 2009

Previously, two events (H15 and B20) of transgenic pepper (Capsicum annuum L.) that enhanced resistance to Cucumber mosaic virus (CMV) by the introduction of CMV coat protein (CP) gene were constructed. Presently, a single copy number of the CP gene was revealed in H15 and B20 by Southern blot. To predict possible unintended effects due to transgene insertion in an endogenous gene, we carried out sequencing of the 5'-flanking region of the CP gene and a Blastbased search. The results revealed that insertion of the transgene into genes encoding putative proteins may occur in the H15 and B20 transgenic event. Mutiplex polymerase chain reaction (PCR) for simultaneous detection and identification of transgenic pepper was conducted with a set of nine primers. Both transgenic event were differentiated from non-transgenic event by the presence of 267 bp and 430 bp PCR products indicative of CP gene specific primer pairs and primer pairs targeting the CP gene and 35S promoter. H15 and B20 uniquely possessed a 390 bp and 596 bp PCR product, respectively. The presence of a 1115 bp product corresponding to intrinsic pepper actin gene confirmed the use of pepper DNA as the PCR template. The primer set and PCR conditions used presently may allow the accurate and simple identification of CMV resistant transgenic pepper.

• 재활복지
• /
• 제21권2호
• /
• pp.29-62
• /
• 2017

본 연구는 성인기 발달장애인의 소득보장과 재산의 보호 및 관리 수단으로서 신탁을 사회복지의 측면에서 연계, 활용하기 위한 "재정계획"과 발달장애인 "신탁"의 주요 운영방안을 모색하고자 하였다. 연구결과 "재정계획"의 운영내용과 관련하여 (1)필요성은 "미래계획을 수립하는데 있어 필요한 재원을 충족시키는 방안 수립", "부모가 살아있는 동안 사후 장애자녀에게 남길 재산에 대한 안전한 관리 방안 수립"이었다. (2)구성내용은 "자립 시 재산 유지 및 사용 방안", "미래에 대한 계획과 꿈", "부모 사후 시 발달 장애인을 위한 유언 및 유산처리 방안" 등 이었으며 (3)장애요인은 "장애인의 재정계획에 대한 국가의 인식부족", 지원의 내용은 "개인의 소득보장과 재산을 관리해줄 수 있는 공신력 있는 기관" 등으로 나타났다. 발달장애인을 위한 "신탁" 운영내용과 관련하여 (1)운영 원칙은 "보편성, 공공성, 안정성, 자기결정, 개별성 보장"이었고, (2)"운영체계는 공공과 민간의 혼합방식", (3)대상자 유형은 "부모가 없거나 돌볼 가족이 없는 장애인으로 당사자의 소득이 있거나 재산을 가진 경우", (4)필요한 지원은 수탁기관에 대한 "재산의 안전한 관리를 위한 관리 감독 시스템", "발달장애인을 위한 의사결정지원 시스템", "일정한도의 신탁재산이 있어도 공적 부조(기초생활수급자, 의료보호 등)의 정부수급 자격의 유지" 등으로 나타났다.

• 대한치과의사협회지
• /
• 제49권1호
• /
• pp.38-44
• /
• 2011

The aim of this study is to evaluate the patient's and clinician's response to rapid oral HIV screening test. Attitude assessment survey was carried out on patients test with rapid oral HIV screening test and clinicians who conducted the test. The patients and clinicians were present at the dental hospital and clinics which were participating in the research project, between March and May 2010. Rapid oral HIV screening test is safe and efficient to the patients. It also demonstrated reliability to accuracy and confidentiality, and high recommendation to rapid oral HIV screening test. The clinician survey revealed high test convenience and high appraisal to patient satisfaction. In conclusion, if rapid oral HIV screening test becomes more common, patients will receive safe and convenient and high reliable HIV infection test, while clinicians will be able to create safe dental care environment.

• Korean Circulation Journal
• /
• 제52권3호
• /
• pp.220-230
• /
• 2022

Background and Objectives: Previous observational studies presented a positive association between alcohol and atrial fibrillation (AF). However, previous studies using genetic polymorphisms on the causal relationship between alcohol consumption and AF have reported conflicting results. This study aimed to evaluate the causality between alcohol consumption and AF using the aldehyde dehydrogenase 2 (ALDH2) rs671 polymorphism, which is the genetic variant with the most potent effect on drinking behavior. Methods: A total of 8,964 participants from the Dong-gu Study were included in the present study. The causal association between alcohol consumption and AF was evaluated through a Mendelian randomization (MR) analysis using the ALDH2 rs671 polymorphism as an instrumental variable. Results: No significant relationship between alcohol consumption and AF was found in the observational analysis. However, the genetic analysis using the ALDH2 polymorphism showed a significant association in men. In the MR analysis, genetically predicted daily alcohol consumption was positively related to AF. Conclusions: MR analysis revealed a significant association between the amount of alcohol consumption and AF, which suggests that the association may be causal.

• 한국식품위생안전성학회지
• /
• 제22권3호
• /
• pp.209-212
• /
• 2007

내분비 교란 물질 중에서 최근에 문제가 되고 있는 물질이 프탈레이트계통 물질로서 플라스틱의 가소제로 사용하고 있는 DEHP와 DBP에 대하여 사람유방암세포 이면서 에스트로젠 의존성을 가지고 있는 MCF-7 세포에서의 세포 증식 정도를 농도 별로 측정하여 두 물질의 에스트로젠 작용가능성에 대하여 조사하였다. 시험물질인 DEHP와 DBP의 MCF-7 세포의 최대 증식 시의 농도를 조사해 본 결과, DEHP는 $17{\beta}-Estradiol$에 비하여 100배정도 높은 농도인 $10^{-7}M$ 에서 최대 증식능력을 보였고, DBP는 10배정도 높은 $10^{-8}M$ 에서 최대 증식능력을 보였다. 최대 증식 능력을 보일 때의 양성대조물질인 $17{\beta}-Estradiol$와 증식 정도 차이를 비교하였을 때에는 DEHP는 양성대조군 대비 87.5%의 증식 정도를 나타내었고, DBP는 73.4%의 증식 정도를 나타내었다 결론적으로 DEHP와 DBP 두 물질 모두 MCF-7 세포의 증식에 영향을 주는 것으로 판단되었으며, 최대 작용농도에 있어서는 DBP>DEHP, 세포 증식 정도에 있어서는 DEHP>DBP 인 것으로 판단되어 진다.

• Korean Journal of Radiology
• /
• 제22권12호
• /
• pp.2094-2123
• /
• 2021

Incidental thyroid nodules are commonly detected on ultrasonography (US). This has contributed to the rapidly rising incidence of low-risk papillary thyroid carcinoma over the last 20 years. The appropriate diagnosis and management of these patients is based on the risk factors related to the patients as well as the thyroid nodules. The Korean Society of Thyroid Radiology (KSThR) published consensus recommendations for US-based management of thyroid nodules in 2011 and revised them in 2016. These guidelines have been used as the standard guidelines in Korea. However, recent advances in the diagnosis and management of thyroid nodules have necessitated the revision of the original recommendations. The task force of the KSThR has revised the Korean Thyroid Imaging Reporting and Data System and recommendations for US lexicon, biopsy criteria, US criteria of extrathyroidal extension, optimal thyroid computed tomography protocol, and US follow-up of thyroid nodules before and after biopsy. The biopsy criteria were revised to reduce unnecessary biopsies for benign nodules while maintaining an appropriate sensitivity for the detection of malignant tumors in small (1-2 cm) thyroid nodules. The goal of these recommendations is to provide the optimal scientific evidence and expert opinion consensus regarding US-based diagnosis and management of thyroid nodules.

• Parasites, Hosts and Diseases
• /
• 제55권3호
• /
• pp.247-254
• /
• 2017

ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera. Comparison between groups was analyzed by the Student's t-test. The positive seroprevalence surged from 14.7% (135/921, 2010), 23.1% (231/993, 2011), 23.6% (222/940, 2012), and 32.1% (269/838, 2013) by ELISA. In contrast, RDT showed a more moderate increasing trend from 21.7% (200/921, 2010), 25.5% (253/993, 2011), 28.9% (272/940, 2012) and 33.1% (277/838, 2013). Discrepancies between ELISA and RDT were noted near the cut-off value. At the OD 0.15-0.24 range, RDT could detect 16.1% (169/1051) more positives, which suggests an early or acute toxoplasmosis, but at the OD 0.25-0.34 range, ELISA could detect 35.9% (92/256) more positives of possible chronic infections. Over the OD > 0.35 ELISA and RDT agreed in the majority of the cases. This surge in seroprevalence may be caused by the organic agriculture in addition to eating behavior or increase in pets among Koreans. These facts may be applied on a full-scale national survey using RDT to supplement ELISA to define the characteristics of the infection.

• 한국미생물·생명공학회지
• /
• 제43권4호
• /
• pp.378-384
• /
• 2015

누룩은 전통주를 빚기 위한 발효제이다. 본 연구의 목적은 누룩 발효시 온도의 영향을 알아보기 위한 것이다. 누룩 발효는 $36^{\circ}C$에서 30일간 발효한 처리구(TN-A)와 $45^{\circ}C$에서 10일간 전발효 후, $36^{\circ}C$에서 20일간 후 발효시킨 처리구(TN-B)로 실험하였다. 누룩 발효시 온도에 따른 ${\alpha}$-amylase, glucoamylase, acidic protease 등 효소활성과 누룩의 대사산물을 측정하였다. 초기 효소활성은 온도에 상관없이 급격하게 증가하였고 발효 3일 후, TN-A 누룩의 효소활성은 일정하게 유지됐으나 TN-B 누룩은 발효기간이 끝나는 동안 점진적으로 이들 활성이 감소하였다. $^1H$-MNR을 이용한 대사체 분석결과, TN-A 누룩은 glucose, glycerol, fructose, mannitol, lactose가 초기에 급격히 증가한 후 감소하였으나, TN-B 누룩은 초기에 대사체들이 감소한 후, 발효기간 동안 일정하게 유지되었다. Glycine, proline, serine 유리아미노산은 TN-B보다 TN-A 누룩에서 보다 높게 나타났다. 밀누룩 발효시 약 $36^{\circ}C$의 일정한 온도는 전분 및 단백질분해 효소의 높은 활성을 유지하는데 적절하였다.

• Journal of Microbiology and Biotechnology
• /
• 제26권6호
• /
• pp.1035-1045
• /
• 2016

To evaluate the effects of lactic acid bacteria (LAB) on Peyer's patch cells, mice were treated with a high dose of kanamycin to disturb the gut microbial environment. The overarching goal was to explore the potential of LAB for use as a dietary probiotic that buffers the negative consequences of antibiotic treatment. In vitro, LAB stimulated the production of immunoglobulin A (IgA) from isolated Peyer's patch cells. Inflammation-related genes (TNF-α, IL-1β, and IL-8) were up-regulated in Caco-2 cells stimulated with lipopolysaccharide (LPS), while tight-junction-related genes (ZO-1 and occludin) were down-regulated; the effects of LPS on inflammatory gene and tight-junction gene expression were reversed by treatment with LAB. Mice treated with a high dose of kanamycin showed increased serum IgE levels and decreases in serum IgA and fecal IgA levels; the number of Peyer's patch cells decreased with kanamycin treatment. However, subsequent LAB treatment was effective in reducing the serum IgE level and recovering the serum IgA and fecal IgA levels, as well as the number of Peyer's patch cells. In addition, ZO-1 and occludin mRNA levels were up-regulated in the ileum tissues of mice receiving LAB treatment. Lactic acid bacteria can enhance the intestinal immune system by improving the integrity of the intestinal barrier and increasing the production of IgA in Peyer's patches. Lactic acid bacteria should be considered a potential probiotic candidate for improving intestinal immunity, particularly in mitigating the negative consequences of antibiotic use.