• Journal of Microbiology
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• v.34 no.1
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• pp.40-48
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• 1996

We have examined the chromatin structure of the HMRE/HSP82 and HMRa/HSP82 allels using three complementary approaches : DNase I chromating footprinting, micrococcal nuclease (MNase) nucleosome-protected ladder assay, and an in vivo E. coli dam methylase accessibility assay. The footprinting results indicate that the promoter and silencer sequences are assembled into nucleoprotein complexes which exhibit no detectable change in structure, despite a 70-fold range in expression levels. In addition, the promoter region of the HMRa/HSP82 allele is cleaved randomly by MNase in all cases, indicating the absence of anonical nucleosomes over this region irrespective of SIR4 or heat-shock. Finally, no discernible difference in the accessibility of the HMRE/HSP82 locus to dam methylase in SIR4 vs. sir4 cells was seenm which again suggests that the chromatin structure of HMRE/HSP82 allele is identical regardless of SIR4. Altogether, our results indicate that in contrast to other observations of the silent mating-type loci, no discernible structural alteration is detected at either HMR/HSP82 allele regardless of SIR genetic background or transcriptional state of the gene.

• Genomics & Informatics
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• v.16 no.4
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• pp.39.1-39.3
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• 2018

The rapid increase in genetic dataset volume has demanded extensive adoption of biological knowledge to reduce the computational complexity, and the biological pathway is one well-known source of such knowledge. In this regard, we have introduced a novel statistical method that enables the pathway-based association study of large-scale genetic dataset-namely, PHARAOH. However, researcher-level application of the PHARAOH method has been limited by a lack of generally used file formats and the absence of various quality control options that are essential to practical analysis. In order to overcome these limitations, we introduce our integration of the PHARAOH method into our recently developed all-in-one workbench. The proposed new PHARAOH program not only supports various de facto standard genetic data formats but also provides many quality control measures and filters based on those measures. We expect that our updated PHARAOH provides advanced accessibility of the pathway-level analysis of large-scale genetic datasets to researchers.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2019.05a
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• pp.169-170
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• 2019

Domestic marine tourism has been greatly influenced by traffic accessibility. The purpose of this study is to provide a way to improve the plan of marine tourism in order for expanding traffic network. In particular, with the successful hosting of the 2018 PyeongChang Winter Olympic Games and the opening of Seoul-Gangneung KTX.

• Proceedings of the Korea Information Processing Society Conference
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• 2007.05a
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• pp.473-476
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• 2007

정보통신기술 특히 인터넷의 급속한 확산으로 웹은 특정계층의 도구가 아니라 모두에게 평등한 보편적 서비스로 자리매김하고 있지만, 장애인 노인 등의 정보취약계층은 이러한 인터넷을 활용하지 못해 불이익을 받는 정보격차(Digital Divide) 현상이 나타나고 있다. 인터넷에 접근하지 못하고 활용하지 못하는 근본적 원인은 웹 접근성(Web Accessibility)의 부재라 할 수 있다. 이에 본 논문에서는 국내외 언론사 홈페이지의 웹 접근성을 평가 비교하여 웹 접근성에 대한 관심을 증가시키고 웹 접근성의 향상 방안을 제시하였다.

• Korean Institute of Interior Design Journal
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• v.25 no.1
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• pp.3-12
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• 2016

This study analyses characteristics of space to facilitate community centering on cases of collective houses in Japan and uses the results as basic data to activate collective houses as an alternative form of housing in our country. To identify the characteristics of space for active communities with collective living space, this study conducted a theoretical examination on collective houses and housing, derived territoriality, accessibility, comfort and safety as factors of community activation and analysed various cases, and the results are presented as follows: First, territoriality is a necessary condition for formation of community, and a confined and physical space. It indicates the proper size of a space and diverse spacial areas which meet the needs of residents with the proper size and amount of space (space which can accommodate diverse generations including women, the aged and the young, flexible space which changes according to the purposes and program, space which supports leisure and hobby activities, and space which can accommodate outsiders). Second, accessibility indicates an environment which supports residents' easy use of space. As the more accessible it is, the higher the frequency of its use, spatial factors (piloti, backyards, benches and tables) should be provided, community space should be arranged in a central or decentralized way and community living space should be made for each floor. As for exits, accessibility should be considered in every way; entrances connected with community spaces, open entrances and entrances accessible from outside or individual living spaces. Third, comfort satisfies the physical and mental needs of residents through separation of floors and flows between the community and individual spaces for privacy and formation of eco-friendly spaces including vegetable gardens, gardens and plant arrangements. Fourth, safety should be considered for a higher quality environment with psychological stability and comfort, for which the following are recommended: installation of hand-rails, removal of thresholds, spacial design for the socially weak, duplex and open type structures for community space, open stages and halls, as well as indoor and outdoor openness through the use of windows.

• Journal of Korean Society of Rural Planning
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• v.13 no.3
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• pp.51-60
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• 2007

In terms of the indicators by the type for the landscape plan per the district nit, geographic ecology indicators were the items such as water, water system, topography and climate, while biological ecology indicators were the items such as green zone size, green zone ratio, biotope size and distribution. The sensory and visual indicators were the items such as view point, view axis and skyline, and the accessibility indicator items for the green zone were applied as the socially used indicators. As for the application method according to each indicator, the water or water system was expressed and applied through the slopes and elevation topographic analysis drawings, the climate was expressed and applied through the main direction of the wind and the cold wind pass alley drawings. As for the green zone size and green zone ratio, the land use according to the status and housing area development was compared to present the measured figures. In case of the biotop size and the distribution item, the range was limited to the preparation of the biotope drawings for the focused preservation. As for the view point and view axis, the view point was selected according to the view point to prepare and apply the view analysis drawing in which the view area to be preserved is analyzed. As for the skyline, its landscape photo was composed with the housing area shape, and then skyline photography was applied as the plan in order to propose and consider the various shapes of the skyline. As for the accessibility to the peen zone, the network drawing was drawn to reflect the users' accessibility and continuity of the green zone formed and preserved according to the land use plan and then the drawing was prepared and proposed as the applicable plan.

• Journal of Families and Better Life
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• v.24 no.6 s.84
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• pp.117-129
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• 2006

The purpose of this study was to provide basic information about hierarchical spatial compositions of lounges and corridors in elderly skilled nursing facilities through the analysis of elderly residents' behaviors. For the study, five researchers observed the behavior of the elderly in the lounges and corridors of five facilities from 10 AM. to 4 PM. at 30-minute intervals for the investigation of hierarchical spatial compositions. And then, the design characteristics of public and semi-public area were analyzed by evaluating their openness, centrality, accessibility, and stimulations. The results of this study were as follows. First, the public area was focused on the lounge, and the semi-public area was formed on the comers of corridors and the parts of lounge walls. The private and semi-private area were distributed to the whole lounges and corridors. Second, in morning time, the semi-public area was concentrated on the wall of lounge instead of on the comers of corridors, and in afternoon, the residents' behaviors relevant to the private and semi-public areas were increased. Especially, the comers of corridors were utilized as the main semi-public areas. Third, the public area could encourage the participation of the elderly residents when it was opened to other areas more than 40% and close to the nursing station. But, the central garden made the accessibility from the elderly private room to the public area not good. The ideal openness of semi-public area was indicated as 25-30%, and its centrality was $10{\sim}15m$ which was a little far from the nursing station. Forth, furniture arrangements gave large influence to the behavior characteristics in the semi-public areas. Especially, sofa arrangements in the comers of corridors increased informal interactions among the elderly. In conclusion, the public area needs high rate of openness and should satisfy both of the centrality and accessibility so that it lead the elderly participations from the private and semi-private areas. The semi-public area should be in a little distance from nursing station and provide with furniture sets for the elderly informal interactions.

• Journal of Korean Society of Rural Planning
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• v.21 no.3
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• pp.145-154
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• 2015

With the rapid change of rural communities and increasing desire for social welfare, the demand for the quality of public spaces and facilities is increasing. However, inefficient location of rural public space and facility has caused problems such as economic inefficiency, inconvenient accessibility, environmental pollution, unsustainable energy use, and disturbance of visual landscape. This study aims to evaluate suitability of public spaces and facilities in three types of rural village: rural downtown village, rural clustered village, and individual village. As a way to measure the suitability of public spaces and facilities, we assessed indicators of suitability in terms of accessibility, usability, biophysical environment, and sustainable management. To evaluate the accessibility, we applied indicators such as distance from residential areas, public transit, market and public facilities. For the evaluation of biophysical environment, we applied indicators such as slope, elevation, aspect, and occurrence of natural disasters. We analyzed digital maps representing the indicators listed above using GIS. We also conducted a survey and face-to-face interviews to evaluate usability and sustainability of management which are not possible to understand through maps. As a result, we identified that suitability of public spaces and facilities in downtown villages is higher than clustered and individual villages. In particular, suitability of infrastructure facilities, social welfare facilities, and income generation facilities in downtown village are higher than those in clustered and individual villages. The suitability of tourism facilities is generally lower than other types of public facility across the three types of villages. Tourism facilities in clustered village have been identified especially unsuitable compared to other types of villages. We expect the outcome of the research is helpful to improve allocation of public spaces and facilities in rural villages.

• Nutrition Research and Practice
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• v.11 no.4
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• pp.340-346
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• 2017

BACKGROUND/OBJECTIVES: Despite the importance of consuming sufficient amounts of vegetables, daily vegetable intake among adolescents in Korea is lower than the current dietary recommendation. The objective of this study was to examine determinants affecting vegetable preference in order to suggest a stage-tailored education strategy that can promote vegetable consumption in adolescents. SUBJECTS/METHODS: Adolescents (n = 400, aged 16-17 years) from two high schools participated in a cross-sectional study. Survey variables were vegetable preference, the social cognitive theory (SCT) and stages of change (SOC) constructs. Based on vegetable preference, subjects were classified into two groups: a low-preference group (LPG) and a high-preference group (HPG). SOC was subdivided into pre-action and action/maintenance stages. To compare SCT components and SOC related to vegetable preference, chi-squared and t-tests, along with stepwise multiple-regression analysis, were applied. RESULTS: In the LPG, a similar number of subjects were classified into each stage. Significant differences in self-efficacy, affective attitudes, and vegetable accessibility at home and school were detected among the stages. Subjects in the HPG were mainly at the maintenance stage (81%), and there were significant differences among the stages regarding self-efficacy, affective attitudes, and parenting practice. In the predictions of vegetable preference, self-efficacy and parenting practice had a significant effect in the "pre-action" stage. In the action/maintenance stage, outcome expectation, affective attitudes, and vegetable accessibility at school had significant predictive value. In predicting the vegetable preference for all subjects, 42.8% of the predictive variance was accounted for by affective attitudes, self-efficacy, and vegetable accessibility at school. CONCLUSION: The study revealed that different determinants affect adolescent vegetable preference in each stage. Self-efficacy and affective attitudes are important determinants affecting vegetable preference. Additionally, school-based nutrition intervention that focuses on enhancing affective attitudes, self-efficacy, and vegetable exposure may constitute an effective education strategy for promoting vegetable consumption among adolescents.

• Journal of Korean Academy of Oral Health
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• v.42 no.4
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• pp.167-174
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• 2018

Objectives: The aim of this study was to analyze the accessibility of dental care services among individuals with precarious employment in South Korea. Methods: We used the $9^{th}$ wave of the Korean Health Panel data (2015) and included 7,736 wage and non-wage earners in our study. We determined precariousness in the labor market as a combination of employment relationship and job income, and categorized individuals based on this into the following four groups: Group A comprising those who report job and income security, Group B comprising those who experience job insecurity alone, Group C comprising those who report a stable job but low income, and Group D comprising those who experience both job and income insecurity. Accessibility to dental care services was determined by experience of unmet dental care needs and unmet dental care needs caused primarily by financial burden. Logistic regression analyses were used to assess the effect of precarious work on access to dental care services. Results: Individuals with job insecurity (Group B; OR=1.445; 95% CI=1.22-1.70) and both job and income insecurity (Group D; OR=1.899; 95% CI=1.61-2.24) were more likely to have unmet needs than the comparison group. Both groups B and D were also 2.048 (95% CI=1.57-2.66) times and 4.435 (95% CI =3.46-5.68) times more likely, respectively, to have unmet dental care needs caused by financial burden. Education status, health insurance, and health status were all also effective factors influencing unmet dental care needs. Conclusions: Unstable employment and low income resulted in diminished access to dental care services. Therefore, governments should consider health policy solutions to reduce barriers preventing individuals with employment and income instability from accessing adequate dental care.