• Journal of radiological science and technology
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• v.44 no.3
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• pp.173-181
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• 2021

The purpose of this study was to reduce dose while maintaining image quality during digital radiographic examination of paranasal sinus by using the automatic exposure control (AEC) system. The tube voltage was set as six stages that increased by about 10 kVp to 70 kVp, 81 kVp, 90 kVp, 102 kVp, 109 kVp and 117 kVp. And then the AEC system conditions were consisted of 9 setting environments, that change mode of the sensitivity (S200, S400, S800) and the density (+2.5, 0, -2.5). We measured automatically exposed tube current (mAs) under 54 conditions with combined these, and assessed SNR and PSNR through the acquired images. In addition, four radiologists performed a qualitative assessment of the acquired images for each combination on a five-point scale of the Likert. As a result, the lowest dose and the highest values of SNR and PSNR in images with a qualitative assessment more than 4 point were the AEC control factors of 90 kVp, S800, D2.5. We applied this condition to the clinical trial, it showed an effect of 83.1% reduction in exposure radiation dose (mR). Therefore, AEC system could be used as dose reduction technology if it understood and used related regulatory factors and physical characteristics.

• BMB Reports
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• v.41 no.9
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• pp.678-683
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• 2008

The initial step during assembly of the hepatitis A virus particle is driven by domain 2A of P1-2A, which is the precursor of the structural proteins. The proteolytic removal of 2A from particulate VP1-2A by an as yet unknown host enzyme presumably terminates viral morphogenesis. Using a genetic approach, we show that a basic amino acid residue at the C-terminus of VP1 is required for efficient particle assembly and that host proteases trypsin and cathepsin L remove 2A from hepatitis A virus particles in vitro. Analyses of insertion mutants in the C-terminus of 2A reveal that this part of 2A is important for liberation of P1-2A from the polyprotein. The data provide the first evidence that the VP1/2A junction is involved in both viral particle assembly and maturation and, therefore, seems to coordinate the first and last steps in viral morphogenesis.

• Fisheries and Aquatic Sciences
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• v.26 no.3
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• pp.204-215
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• 2023

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 ㎍ of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10^-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.11a
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• pp.619-622
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• 2005

This study concerns the new strength equation of concrete by ultrasonic pulse velocity test. There are not only few estimate strength equations of concrete by ultrasonic pulse velocity test, but also many problems to apply them because of time,. cost, easiness, structural damage, reliability and so on. For this study, there performed a series of test and proposed equations as follows; Linear: ${\Large f}_{ck}=-193.15+60.97Vp\;r^2=77.9\%$ Quadratic : ${\Large f}_{ck}=276.85-189.64Vp+33.22Vp^2\;r^2=80.3\%$ here, $f_{ck}$ : Estimated compressive strength of concrete by MPa Vp : Ultrasonic Pulse Velocity of concrete by km/sec

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1960-1965
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• 2015

Rabbit hemorrhagic disease virus (RHDV) is highly contagious and often causes fatal disease that affects both wild and domestic rabbits of the species Oryctolagus cuniculus. A highly pathogenic RHDV variant (RHDVa) has been circulation in the Korean rabbit population since 2007 and has a devastating effect on the rabbit industry in Korea. A highly pathogenic RHDVa was isolated from naturally infected rabbits, and the gene encoding the VP60 protein was cloned into a baculovirus transfer vector and expressed in insect cells. The hemagglutination titer of the Sf-9 cell lysate infected with recombinant VP60 baculovirus was 131,072 units/50 μl and of the supernatant 4,096 units/50 μl. Guinea pigs immunized twice intramuscularly with a trial inactivated RHDVa vaccine containing recombinant VP60 contained 2,152 hemagglutination inhibition (HI) geometric mean titers. The 8-week-old white rabbits inoculated with one vaccine dose were challenged with a lethal RHDVa 21 days later and showed 100% survival rates. The recombinant VP60 protein expressed in a baculovirus system induced high HI titers in guinea pigs and rendered complete protection, which led to the development of a novel inactivated RHDVa vaccine.

• Journal of radiological science and technology
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• v.32 no.3
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• pp.299-306
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• 2009

Purpose : To compare radiation dose for coronary CT angiography (CTA) obtained with 6 examination protocols such as a retrospectively ECG gated helical scan, a prospectively ECG gated sequential scan, low kVp technique, and cardiac dose modulation technique. Materials and Methods : Coronary CTA was performed by using 6 current clinical protocols to evaluate effective dose and organ dose in primary beam area with anthropomorphic female phantom and glass dosimetric system in 64 channel multi-detector CT. After acquiring topograms of frontal and lateral projection with 80 kVp and 10 mA, main coronary scan was done with 0.35 sec tube rotation time, 40 mm collimation ($0.625\;mm{\times}64\;ea$), small scan field of view (32 cm diameter), 105 mm scan length. Heart beat rate of phantom was maintained 60 bpm in ECG gating. In constant mAs technique 120 kVp, 600 mA was used, and 100 kVp for low kVp technique. In a retrospectively ECG gated helical CT technique 0.22 pitch was used, peak mA (600 mA) was adopted in range of $40{\sim}80%$ of R-R interval and 120mA(80% reduction) in others with cardiac dose modulation. And 210 mAs was used without cardiac dose modulation. In a prospectively ECG gated sequential CT technique data were acquired at 75% R-R interval (middle diastolic phase in cardiac cycle), and 120 msec additional padding of the tube-on time was used. For effective dose calculation region specific conversion factor of dose length product in thorax was used, which was recommended by EUR 16262. Results : The mean effective dose for conventional coronary CTA without cardiac dose modulation in a retrospectively ECG gated helical scan was 17.8 mSv, and mean organ dose of heart was 103.8 mGy. With low kVp and cardiac dose modulation the mean effective dose showed 54.5% reduction, and heart dose showed 52.3% reduction, compared with that of conventional coronary CTA. And at the sequential scan(SnapShot pulse mode) under prospective ECG gating the mean effective dose was 4.9 mSv, this represents an 72.5% reduction compared with that of conventional coronary CTA. And heart dose was 33.8 mGy, this represents 67.4% reduction. In the sequential scan technique under prospective ECG gating with low kVp the mean effective dose was 3.0 mSv, this represents an 83.2% reduction compared with that of conventional coronary CTA. And heart dose was 17.7 mGy, this represents an 82.9% reduction. Conclusion : In coronary CTA at retrospectively ECG gated helical scan, cardiac dose modulation technique using low kVp reduced dose to 50% above compared with the conventional helical scan. And the prospectively ECG gated sequential scan offers substantially reduced dose compared with the traditional retrospectively ECG gated helical scan.

• The Journal of Korean Society of Virology
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• v.28 no.2
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• pp.165-174
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• 1998

Characterizations of the VP4 (P type) and VP7 (G type) genes of Korean isolates of bovine rotavirus were performed using RT-PCR/RFLP and nucleotide sequencing analysis. After RT-PCR amplification of partial length (1094bp) of the VP4 and full length (1062bp) of the VP7 genes, amplified PCR products were digested with restriction endonucleases and digestion patterns were compared with those of reference rotaviruses. With the VP4 genes, four RFLP (A-D) profiles were observed; three (A, Band C) were the same as those of bovine rotavirus NCDV (P[1]), IND (P[5]) and B223 (P[11]), respectively. Profile D was the same as that of porcine rotavirus OSU (P[7]). With the VP7 genes, five RFLP profiles (I-V) were observed; three of them (I, II and III) were the same as those of bovine rotavirus NCDV (G6), Cody 1-801 (G8), and B223 (G10), respectively. Profile IV and V were atypical to those of reference bovine rotaviruses used in this study. These two profiles were identified as G6 and G5, respectively, after analyzing and comparing the nucleotide sequences. The G typing analysis revealed that 61.9% (26/42) were G6, which included G6 subtype; 28.6% (12/42) were G5; 7.1% (3/42) were G10; 2.4% (1/42) were G8. The P typing analysis revealed that 54.8% (23/42) were P[5]; 28.6% (12/42) were P[7]; 11.8% (5/42) were [11]; 4.8% (2/42) were P[1]. Our results showed that G6/P[5] were the most prevalent rotaviruses in diarrheic calves in Korea. Also, this is the first report that G5/P[7] rotaviruses were identified from cattle with diarrhea.

• 한국해양학회지
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• v.20 no.2
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• pp.10-21
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• 1985

The sound velocity (compressional wave) and attenuation coefficient in the marine surface sediments in the nearshore areas off the Pohang, Pusan, Yeosu and Kunsan were investigated in terms of the geotechnical properties of the marine surface sediments in the water depth range of 10-50 meters. The marine surface sediments in the study areas are variable, that is, sand to clay. Due to the various four different study area, the sound velocities and attenuation coefficients in the surface sediment facies vary 1,44m/sec to 1,510m/sec in velocity and 0.82dB/m to 3.70dB/m in coefficient respectively. In fact, the sound velocity increases with increasing of density and mean grain sizes of the sediments, and however, with decreasing of porosith. The correlation equations between the sound velocith and geotechnical properties of mean grain size, density, and porosity were expressed as the following: Vp=1512.28406-9.16083(Mz)＋0.20795(Mz)$\^$2/, Vp=1876.15527-597.50397(d)＋210.48375(d)$\^$2/, Vp=1559.47217-2.09266(n)$\^$2/. where Vp is sound velocity, Mz is mean grain size, d is density, and m is porosity, respectively. However, the relationship between the attenuation and geotechnical properties were different from that of sound velocity and geotchnical properties. Furthermore, the correlation equations between attenuation coefficient and geotechnical properties were expressed as the following: a=1.85217＋0.67197(Mz)-0.09035 (Mz)$\^$2/, a=48.87859＋58.21721(d)-16.3.143(d)$\^$2/, a=2.06765＋0.07215(n)-0.00111(n)$\^$2/, where a is attenuation coefficient. The high attenuation appeared in the silty sand through fine sand facies in sediment and k values in these facies were in the range of 0.86 to 0.89 dB/m/KHz.

• Journal of radiological science and technology
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• v.38 no.4
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• pp.421-427
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• 2015

In this study, we have observed the change of the Hounsfield (HU) in the alteration of by changing in size of physical area and setting size of region of interest (ROI) at focus on kVp and mAs. Four-channel multi-detector computed tomography was used to get transverse axial scanning images and HU. Three dimensional printer which is type of fused deposition modeling (FDM) was used to produce the Phantom. The structure of the phantom was designed to be a type of cylinder that contains 33 mm, 24 mm, 19 mm, 16 mm, 9 mm size of circle holes that are symmetrically located. It was charged with mixing iodine contrast agent and distilled water in the holes. The images were gained with changing by 90 kVp, 120 kVp, 140 kVp and 50 mAs, 100 mAs, 150 mAs, respectively. The 'image J' was used to get the HU measurement of gained images of ROI. As a result, it was confirmed that kVp affects to HU more than mAs. And it is suggested that the smaller size of physical area, the more decreasing HU even in material of a uniform density and the smaller setting size of ROI, the more increasing HU. Therefore, it is reason that to set maximum ROI within 5 HU is the best way to minimize in the alteration of by changing in size of physical area and setting size of region of interest.

• Journal of radiological science and technology
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• v.8 no.2
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• pp.3-9
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• 1985

From the study on the chest radiologic equipments and situations of university hospitals, general hospitals, and radiologic clinics located in Seoul area and Choong-cheong province, the author got conclusions as follows : 1. The rectfication methods of the equipments were employing mainly single or three-phase full wave, and 78% of the examined were using single phase full wave. 2. The focal sizes in case of small focus were less than 1.0mm in chest-only use, and more than 1.0mm in 81% of combined use. The focal sizes in case of large focus were more than 1.6mm in 78% of the total. 3. Maximum ratings of x-ray tubes were 47% at 125 kVp maximum tube voltage and 41% at 150kVp. The maximum tube currentes higher than 500mA were shown in 72% of all the examined. And the maximum exposure time was the range of 5-8sec. 4. Grid was not used in the 66% of all the hospitals. Equipment for chest-only was employing 10:1 grid ratio and the 2.0-2.9mmAl of total filtration formed 38% of all the examined. 5. In the processing time of exposed film, 68% of all was 90sec, and only 20% of all the examined used optimum developing temperature according to this condition. 6. In the radiographic conditions, more than 50% used low tube voltages lower than 70 kVp and only 3% of all the equipments used high tube voltages higher than 100 kVp. The 46.9% of all the equipments 300mA, and 53% of all used shorter exposure time less than 1/20(0.05) sec. 7. In the monthly average number of radiographs, 44% of all was x-ray the radiographs.