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Disaccharide Synthesis using E. coli UDP-glucose regeneration system (대장균의 UDP-glucose regeneration 시스템을 이용한 이당류 합성에 관한 연구)

  • Oh, Jeong-Seok
    • KSBB Journal
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    • v.23 no.6
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    • pp.474-478
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    • 2008
  • UDP-glucose regeneration system using metabolic engineeringis unique and efficient strategy for oligosaccharide synthesis. To exploit the efficient UDP-glucose regeneration system, we introduced four enzymes, which would be important in partitioning the flux of UDP regenerationsuch as UDP-glucose pyrophosphorylase, UDP-Kinase gene, UDP-galactose 4-epimerase, and $\beta$-1, 4-galactasyltrasnsferase, into E. coli AD202. To determine the optimal expression level for UDP-regeneration, LacNAc concentration was compared depending on IPTG concentration. 0.5 mM IPTG induction showed the higher oligosaccharides synthesis. Using metabolic engineering under optimal IPTG induction, LacNAc synthesis of AD202/pQNGLU increased until 16 h and showed the 1.34 mM. This concentration is 10 times higher than that of control strain at same reaction time. Lactose of AD202/pQNGLU showed the maximum synthesis of 0.39 mM at 16 h and showed the 2.6 times higher than that of control strain.

Effect of Glycyrrhizae Radix on the Glucuronidation in Rat Liver

  • Moon, Aree;Lee, Mi-Kyung;Kim, Seung-Hee;Kim, Young-Choong;Lee, Song-Deuk
    • Archives of Pharmacal Research
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    • v.18 no.5
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    • pp.320-324
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    • 1995
  • Pretreatment of Glycyrrhizae Radix(GR) to male Sprague-Dawley rats was demonstrated to increase excretion of acetaminophen-glucuronide ocnjugate when bile nad urine were assayed after administration of acetaminophen. In order to study the effect of GR on the glucuronidation in rats, we examined enzymatic activities of hepatic UDP-glucuronosyl-transferases (UDP-GT1 and UDP-GT2) and intracellular concentrations of hepatic UDP-glucuronic acid (UDP-GA), upon the administration of GR (1 g/kg body weight, p.o.) or glycyrrhizin (23 mg/kg body weight, p.o.) a major component of GR, for 6 days. GR and glycyrrhizin caused increases in specific activities of UDP-GT2 111% and 96% respectively. Specific activity of UDP-GT1 was increased 25% by GR treatment whereas it was not significantly increased by glycyrrhizin. Concentrations of UDP-GA were increased 257% by GR and 484% by glycyrrhizin. These data indicate that GR activated glucuronidation and thus suggest the possibility that GR may influence detoxification of xenobiotics in rat liver.

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Implementation & Analysis of SAN based on UDP for Cluster Web Server (클러스터 웹서버를 위한 UDP/IP기반 SAM의 구현 및 성능 분석)

  • 이주평;박규호
    • Proceedings of the Korean Information Science Society Conference
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    • 2002.10e
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    • pp.532-534
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    • 2002
  • 본 논문에서는 UDP/IP기반 SAN의 구현을 소개하였고 TCP/IP기반 SAN 및 로컬디스크와의 성능 비교를 통해 UDP/IP기반 SAN이 클러스터 웹서버에서 사용될 수 있는 가능성을 보였다. 실험결과 UDP/IP기반 SAN은 TCP/IP기반 SAN의 경우보다 약 20%정도 성능이 우수함을 볼 수 있다. 이는 UDP의 경우 TCP의 프로토콜 오버헤드가 없고 slow start의 영향을 받지 않으며 ACK를 기다릴 필요가 없기 때문이다.

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Effect of Nitrogen Source on the Change of UDP-glucose and ${\beta}$-1,3-glucan Concentration

  • Park, Yang-Ho;Lee, Jung-Heon
    • KSBB Journal
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    • v.21 no.5
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    • pp.366-370
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    • 2006
  • In this research, analysis of UDP-glucose a precursor of ${\beta}$-1,3-glucan by high performance liquid chromatography(HPLC) was established using a reversed phase system. One of key metabolite UDP-glucose was selected and its concentration changes was measured with the change of fermentation conditions. The effects of fermentation conditions with/without nitrogen source for cell growth on ${\beta}$-1,3-glucan production were dependent on the UDP-glucose concentration. The UDP-glucose was synthesized rapidly during cell exponential growth period and maintained high during ${\beta}$-1,3-glucan production period. The UDP-glucose concentration was higher for ${\beta}$-1.3-glucan production fermentor than that for cell growth fermentor. The ${\beta}$-1,3-glucan production was optimal at pH 5.5 and synthesis of ${\beta}$-1,3-glucan was greatest at pH 5.5.

Capacity Analysis of Internet Protocol Television (IPTV) over IEEE 802.11ac Wireless Local Area Networks (WLANs)

  • Virdi, Chander Kant;Shah, Zawar;Levula, Andrew;Ullah, Imdad
    • International Journal of Computer Science & Network Security
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    • v.22 no.2
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    • pp.327-333
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    • 2022
  • Internet Protocol Television (IPTV) has emerged as a personal entertainment source for home users. Streaming IPTV content over a wireless medium with good Quality of Service (QoS) can be a challenging task as IPTV content requires more bandwidth and Wireless Local Area Networks (WLANs) are susceptible to packet loss, delay and jitter. This research presents the capacity of IPTV using User Datagram Protocol (UDP) and TCP Friendly Rate Control (TFRC) over IEEE 802.11ac WLANs in good and bad network conditions. Experimental results show that in good network conditions, UDP and TFRC could accommodate a maximum of 78 and 75 Standard Definition Television (SDTV) users, respectively. In contrast, 15 and 11 High-Definition Television (HDTV) users were supported by UDP and TFRC, respectively. Performance of UDP and TFRC was identical in bad network conditions and same number of SDTV and HDTV users were supported by TFRC and UDP. With background Transmission Control Protocol (TCP) traffic, both UDP and TFRC can support nearly the same number of SDTV users. It was found that TFRC can co-exist fairly with TCP by giving more throughput to TCP unlike UDP.

Implementation and Performance Analysis of UDP/IP Header Compression Protocol in Wired Networks (유선망에서의 UDP/IP 헤더압축 프로토콜의 구현 및 성능분석)

  • 나종민;이종범;이인성;신병철
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.6
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    • pp.1076-1085
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    • 2004
  • Recently, the demands for real-time service and multimedia data are rapidly increasing. There are significant redundancies between header fields both within the same packet header and in consecutive packets belonging to the same packet stream. And there are many overheads in using the current UDP/IP protocol. Header compression is considered to enhance the transmission efficiency for the payload of small size. By sending the static field information only once initially and by utilizing dependencies and predictability for other fields, the header size can be significantly reduced for most packets. This work describes an implementation for header compression of the headers of IP/UDP protocols to reduce the overhead on Ethernet network. Typical UDP/IP Header packets can be compressed down to 7 bytes and the header compression system is designed and implemented in Linux environment. Using the Header compression system designed between a server and clients provides have the advantage of effective data throughput in network. Since the minimum packet size in Ethernet is 64 bytes, the amount of reduction by header compression in practical chatting environment was 6.6 bytes.

The Mechanism for TCP-UDP Fairness Improvement in the Assured Service of the Diffserv Network (차등 서비스 네트워크의 확신 서비스에서 TCP-UDP 공평성 향상 기법)

  • 모상덕;정광수
    • Proceedings of the Korean Information Science Society Conference
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    • 2004.04a
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    • pp.481-483
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    • 2004
  • 본 논문에서는 차등 서비스 네트워크(Diffserv network)의 확신 서비스(assured service)에서 발생할 수 있는 TCP와 UDP 플로우 사이의 공명성 문제를 해결하기 위한 TRBD(Target Rate-based Dropping) 기법을 제안한다. 차등 서비스 네트워크의 확신 서비스에서의 공평성 향상에 대한 기존 연구는 마킹 확률을 변경하여 공평성을 향상시키는 연구가 중심이 되어 왔다. 이러한 연구들은 코어노드에서의 큐 관리를 위하여 주로 MRED(Multiple RED)골 이용하기 때문에 TCP 및 UDP, 목표 전송률 등에 대한 고려가 부족하다. 본 논문에서 제안하는 TRBD 기법은 코어노드에서 목표 전송률에 따라 UDP의 폐기 확률을 조정하여 TCP-UDP 공평성 및 목표 전송률을 고려한 공평성을 향상시킬 수 있다. ns 시뮬레이터를 이용한 실험을 통하여 TRBD 기법의 TCP-UDP 공평성 향상을 확인하였다.

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Generation of Gate-level Models Equivalent to Verilog UDP Library (Verilog UDP Library의 등가 게이트수준 모델 생성)

  • 박경준;민형복
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.40 no.1
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    • pp.30-38
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    • 2003
  • UDP library of Verilog HDL has been used for simulation of digital systems. But it takes a lot of time and efforts to generate a gate-level library equivalent to the UDP library manually due to the characteristic of UDP that does not support synthesis. It is indispensable to generate equivalent gate-level model in testing the digital systems because fault coverage can be reduced without the equivalent gate-level models. So, it is needed to automate the process of generating the equivalent gate-level models. An algorithm to solve this problem has been proposed, but it is unnecessarily complex and time-consuming. This paper suggests a new improved algorithm to implement the conversion to gate-level models, which exploits the characteristic of UDP Experimental results are demonstrated to show the effectiveness of the new algorithm.

UDP with Flow Control for Myrinet (Myrinet을 위한 흐름 제어 기능을 갖는 UDP)

  • Kim, Jin-Ug;Jin, Hyun-Wook;Yoo, Hyuck
    • Journal of KIISE:Information Networking
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    • v.30 no.5
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    • pp.649-655
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    • 2003
  • Network-based computing such as cluster computing requires a reliable high-speed transport protocol. TCP is a representative reliable transport protocol on the Internet, which implements many mechanisms, such as flow control, congestion control, and retransmission, for reliable packet delivery. This paper, however, finds out that Myrinet does not incur any packet losses caused by network congestion. In addition, we ascertain that Myrinet supports reliable and ordered packet delivery. Consequently, most of reliable routines implemented in TCP produce unnecessarily additional overheads on Myrinet. In this paper, we show that we can attain the reliability only by flow control on Myrinet and propose a new reliable protocol based on UDP named RUM (Reliable UDP on Myrinet) that performs a flow control. As a result, RUM achieves a higher throughput by 45% than TCP and shows a similar one-way latency to UDP.

The Study of the Performance Improvement of UDP Packet Loss affected by TCP Flows (TCP Flows의 영향하에서 UDP 패킷손실을 줄이는 방법에 관한 연구)

  • 조기영;문호림;김서균;남지승
    • Proceedings of the IEEK Conference
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    • 1999.11a
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    • pp.1061-1064
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    • 1999
  • UDP has likely been used for real-time applications, such as video and audio. UDP supplies minimized transmission delay by omitting the connection setup process, flow control, and retransmission In general, more than 80 percent of the WAN resources are occupied by Transmission Control Protocol(TCP) traffic as opposed to UDP's simplicity, TCP adopts a unique flow control in this paper, I report new methods to minimize a udp packet loss considering TCP flow control on the real-time application the better performance of real time application can be obtained when they reduce a packet size and FIFO buffer scheduling method competing with TCP bandwidth for the bandwidth and buffering.

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