• 생명과학회지
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• 제31권11호
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• pp.1019-1027
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• 2021

천연물에서 피부 미백 효능을 가진 화합물을 개발하기 위한 노력이 많이 이루어지고 있다. 꽃송이 버섯(Sparassis crispa)은 β-glucan의 함량이 건조중량의 40% 이상으로서 항암과 면역증강 효과가 있는 것으로 입증되어 약용으로도 인정받고 있다. 이 연구에서는 S. crispa β-glucan의 멜라닌 생합성 및 tyrosinase 활성억제 효능을 확인함으로써 피부 미백제로서의 활용 가능성을 평가하고자 하였다. 외부 자극제인 α-melanocyte stimulating hormone (α-MSH)와 S. crispa β-glucan (10, 100, 1,000 ㎍/ml)을 B16F1 melanoma 세포에 투여하여 멜라닌 생성량과 tyrosinase 활성도를 측정하였다. Tyrosinase, tyrosinase related protein-1 (TRP-1), TRP-2, microphthalmia-associated transcription factor (MITF)의 발현정도에 대해서는 western blot으로 분석하였다. S. crispa β-glucan을 10, 100, 1,000 ㎍/ml의 농도로 투여하였을 때 α-MSH 만 투여군에 비하여 멜라닌 생성이 각각 13.9%, 18.7%, 39.5% 감소하였다. S. crispa β-glucan을 10, 100, 1,000 ㎍/ml의 농도로 투여하였을 때 β-glucan을 투여하지않은 α-MSH 유도군에 비하여 tyrosinase 활성도는 각각 15.6%, 26.9%, 43.2% 억제되었다. 또한 tyrosinase와 TRP-1, TRP-2, MITF 단백 발현도 효과적으로 감소시켰다. 본 연구 결과에 따르면 S. crispa β-glucan은 MITF 발현을 억제함으로써 tyrosinase 발현을 감소시켜 멜라닌 생성을 억제시킨 것으로 판단된다. 따라서 S. crispa β-glucan은 미백제로서 유용하게 활용할 수 있을 것으로 생각한다.

• 한국환경보건학회지
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• 제23권2호
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• pp.12-23
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• 1997

Potassium dichromate(PD, 500 ppm, 500 ppm), potassium chromate(PC, 500 ppm, 500 ppm), cobalt chloride(CC, 100 ppm, 10 ppm), methylmercuric chloride(MC, 100 ppm, 10 ppm)을 처리한 배지에 E. coli와 B.subtilis를 배양하는 동안에 당지질과 galactose의 생합성 및 지방산 조성을 대조구와 비교 분석하였다. 금속화합물 처리구에서의 생장과 MGDG, DGDG의 함량 및 total lipid는 대조구에 비해 감소하였다. 또한 당지질 형성에 이용된 galatose의 함량은 억제되었다. E. coli와 B. subtilis에서 MGDG와 DGDG 합성에 이용된 주요 지방산은 다양한 변화를 나타내었다.

• The Plant Pathology Journal
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• 제15권1호
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• pp.34-37
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• 1999

Cymbidium mosaic virus (CyMV) was purified from CyMV infected orchid plant leaves by Sephacryl S-500 HR column chromatography. Partial purification was done by solubilization with Triton X-100 (alkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG 6,000) followed by ultracentrifugation on 30% sucrose cushion. Based on the spectrophotometric analysis, 33 mg of CyMV could be obtained form 100 g of CyMV-infected orchid plant leaves. The purified CyMV represented one distinct homogeneous band by SDS-PAGE, and electron microscopy revealed that it was highly homogeneous and not fragmented. Bioassay demonstrated that the purified CyMV had a normal infectivity to Chenopodium amaranticolor and orchid plants. Based on these results, the purification method in this work could be served as an improved method for the purification of CyMV and similar viruses with good yield, high purity and native integrity.

• Restorative Dentistry and Endodontics
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• 제20권2호
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• pp.538-548
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• 1995

Calcium hydroxide has been used not only as pulp capping and pulpotomy agents in the operative dentistry, but dressing and temporary filling materials in root canal treatment. Calcium hydroxide was known to stimulate odontoblast to produce new reparative dentin and to eliminate microorganims effectively in the infected root canals. The purpose of this study was to evaluate the effect of calcium hydroxide solution on cultured L929 cells and its antibacterial effect on several streptococci. Calcium hydroxide solution (0.121g/100ml) was added to L929 cells and cell viability was measured using 3-(4,5-dimethylthiazol-2-yl) -2,5-dimethyltetrazolium bromide (MTT) and neutral red (NR) dye. Calcium hydroxide solution (20, 40, 60, 80, 100 and $150{\mu}l$) was added to L929 cells in 96-well microplates for 1, 4 and 24 hours respectively. Cell viability was gradually decreased when the volume and exposure time of calcium hydroxide solution were increased. When $150{\mu}l$ of calcium hydroxide was applied to L929 cells for 24 hours, there was more than fifty percent reduction of cell viability. Calcium hydroxide solution (20g/100ml) showed antibacterial effect against S. uberis, S. intermedius and S. mitis after thirty-second exposure. But 0.121g/100ml concentration of cacium hydroxide solution exhibited no antibacterial effect on six streptococci after one-hour exposure.

• 한국염색가공학회지
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• 제17권1호
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• pp.38-44
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• 2005

Oxidation method was used to extract S-keratose from wool. Wool was treated with performic acid and soluble fraction(S-keratose) was collected by evaporating the solvent. S-keratose and Nylon 6 were dissolved in formic acid at the ratio of 100/0, 80/20, 50/50, 20/80, and 0/100, and S-keratose/Nylon 6 web of sub-micron size was made by electro-spinning technique. SEM, EA, FT-IR, XRD, and TGA were used to characterize the properties of S-keratose/Nylon 6 solutions and electrospun fibers. As the Nylon 6 content increased, viscosity, conductivity of the electrospinning solution and the diameter of spun fiber increased. Electrospun nonwoven webs have the same S-keratose/Nylon 6 ratios of the spinning solutions. The crystalline structures of S-keratose and Nylon 6 existed separately in the electrospun webs. Thermal stability of the webs increased due to Nylon 6 content.

• 분석과학
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• 제9권1호
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• pp.107-111
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• 1996

Bipolaris cynodontis의 배양 추출물로부터 이탈리안 라이그라스 뿌리의 생육저해 활성을 나타내는 물질을 분리 정제한 다음에 2차원 NMR을 포함한 각종 기기분석을 이용하여 구조를 결정하였다. 이 화합물의 분자식은 $C_{24}H_{30}O_5$ 이었으며 세스퀴테르펜류 화합물로 이탈리안 라이그라스 및 벼와 같은 벼과식물에 대해서 100ppm에서 100%의 활성을 나타내는 것으로 보아 병원균의 병징 발현에 관여하는 것으로 보여진다.

• Bulletin of the Korean Chemical Society
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• 제35권12호
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• pp.3567-3570
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• 2014

The most stable adsorption structures and energies of four tautomers of 3-methyl-5-pyrazolone (keto-1, enol-1, keto-2, and enol-2) on Ge(100) surfaces were investigated using density functional theory (DFT) calculations. The enol-1, keto-2, and enol-2 tautomers, but not the keto-1 tautomer, were found to exhibit stable adsorption structures on the Ge(100)-$2{\times}1$ surface. Of these three adsorption structures, that of enol-2 is the most stable.

• 한국식생활문화학회지
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• 제30권1호
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• pp.77-85
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• 2015

This study investigated the physicochemical properties and antioxidative activity of soymilk added with buckwheat sprout (SBS), and sensory properties of SBS were analyzed with control soymilk (CS). Moisture content decreased while protein, ash, and lipid contents increased according to content of buckwheat sprout. Solids content and viscosity tended to increase with addition of buckwheat sprout from 0% (CS) to 2.5% (2.5SBS), 3.5% (3.5SBS), and 4.5% (4.5SBS). SBS was found the significant pH drop from 7.08 to 6.43, 6.34, and 6.21. Suspension stability of soymilk slightly decreased with addition of buckwheat sprout. Hunter's color value L of SBS decreased while a and b values increased in comparison with CS. Rutin content was measured 23.78 mg/100 g (2.5SBS), 39.68 mg/100 g (3.5SBS), and 44.80mg/100 g (4.5SBS). Vitamin C content in SBS was higher than CS. Daidzin content increased as buckwheat sprout was added. Total phenolic content increased from 100.95 mg/100 g (CS) up to 315.71 mg/100 g (4.5SBS). For free radical scavenging activity, SBS was significantly higher than CS. In conclusion, SBS can be used as a functional food with higher amounts of rutin, vitamin C, total phenolics, and daidzin. 2.5SBS and 3.5SBS also showed better overall quality characteristics.

• 한국식품과학회지
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• 제24권4호
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• pp.382-386
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• 1992

열수출시 미역 추출액의 품질 특성에 미치는 온도의 영향을 조사하였다. 추출액의 고형분, 단백질의 농도와 수율은 초기추출시간 동안 추출 온도 $50^{\circ}C$에서 $100^{\circ}C$로 증가할수록 높아졌다. 그러나 상징액율은 $50^{\circ}C$와 $100^{\circ}C$에서 가장 낮은 값을 보였으며 $100^{\circ}C$에서 1시간 추출할 때 고형분과 단백질 수율은 각각 21.56%와 4.76%였다. 점도는 $100^{\circ}C$에서 2시간 추출할 때 가장 높았으며 다른 온도의 추출액 점도에 비해 $2.5{\sim}4.0$배 높았다. 탁도는 추출 1시간 이후 차츰 감소하였으며 고형분과 단백질의 초기 추출속도에서 계산된 활성화에너지는 1.18과 3.9 Cal/mole이었다.

• 한국복식학회:학술대회논문집
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• 한국복식학회 2002년도 International Costume Conference
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• pp.100-100
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• 2002