• 대한의생명과학회지
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• 제27권2호
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• pp.95-98
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• 2021

The S100 family proteins act as inducers of cancer cell apoptosis and inflammatory mediators. This study examined the pro-apoptotic mechanism caused by S100A8 and S100A9 in human FIP1L1-PDGFRα-positive eosinophilic leukemia cells. S100A8 and S100A9 elicited the death of EoL-1 cells in a time and dose-dependent manner. The activation of PDGFRα was suppressed by a decrease in PDGFRα after treatment with S100A8 and S100A9. Cycloheximide, a translation inhibitor, suppressed PDGFRα expression from 1 h to 5 h, and a co-treatment with S100A8 and S100A9 boosted the decrease in expression. The phosphorylation and expression of STAT5 decreased after treatment with S100A8 and S100A9 in EoL-1 and imatinib-resistant (EoL-1-IR) cells. S100A8 and S100A9 induced the chemotaxis of EoL-1 cells but did not affect the chemoattraction of EoL-1-IR. These findings indicate the cell death mechanism due to S100 family proteins and the development of leukemia therapy using S100A8 and S100A9.

• 대한의생명과학회지
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• 제23권1호
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• pp.44-47
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• 2017

S100A8 and S100A9 are associated with myeloid cell differentiation, chemotactic activities, adhesion of neutrophils, and apoptosis. In this study, we investigated the contribution of S100A8 and S100A9 to differentiation of the human eosinophilic leukemia cell line, EoL-1. S100A8 and S100A9 increased the number of vacuole per one cell and the protein expression of EPO and MBP. Rottlerin, an inhibitor of protein kinase C delta ($PKC{\delta}$), inhibited the EoL-1 cell differentiation induced by S100A8 and S100A9. These results suggest that S100A8 and S100A9 may regulate the differentiation of eosinophilic progenitors. Moreover, these findings may shed light on elucidation of eosinophil differentiation due to S100 proteins.

• 대한의생명과학회지
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• 제24권2호
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• pp.134-137
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• 2018

S100A8 and S100A9 are involved in pathogenesis of cancer by induction or inhibition of cancer as well as inflammation. In this study, we investigated the association of S100A8 and S100A9 with pathogenesis of leukemia using human monocytic leukemia cells, THP-1. The expression of TLR4, which is a known receptor of S100A8 and S100A9, was examined by using flow cytometry and Western blotting. THP-1 cells have high surface and cytosol expression of TLR4. S100A8 and S100A9 suppressed the cell survival, and this suppression was found to be associated with apoptosis because they increased the number of apoptotic cells in a dose- and a time-dependent manners. However, S100A8 and S100A9 had no effect on the survival and apoptosis of monocytes isolated from the peripheral blood. We next examined the apoptotic effect of lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA), which are other ligands of TLR4, in THP-1 cells. Lipopolysaccharide had no effect on cell survival, but MPLA is effective on the cell apoptosis. These results suggest that S100A8 and S100A9 may regulate leukemia cell survival via TLR4, which is an essential receptor in the pro-apoptotic mechanism induced by S100A8 and S100A9. These findings may shed light on development of a possible therapeutic drug for leukemia treatment.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2018년도 춘계학술대회
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• pp.87-88
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• 2018

본 연구에서는 국제수로기구(IHO)에서 제정한 S-100/10X 표준의 지속적 안정화를 위해 S-100 표준화 등록소(S-100 GI Registry)를 신규로 구축하였다. 이를 통하여 S-100 기반의 다양한 제품표준 사양에서 사용되는 피쳐 정보와 심볼을 체계적으로 관리하고 이용할 수 있을 것으로 예측된다.

• 생물정신의학
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• 제14권3호
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• pp.177-183
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• 2007

목 적: 성상세포(astrocyte)에서 생산되어 신경세포의 증식과분화에 관여하는 S100B 단백이 정신분열병의 진행과 증상론과 관련이 있다는 연구가 계속 진행되고 있다. 이에 정신분열병 환자와 정상대조군의 혈청 S100B 농도를 비교하고, 정신분열병 환자의 증상 양상과 S100B 농도와의 연관성을 연구하였다. 방 법: DSM-IV-TR 진단 기준에 따라 정신분열병으로 진단받은 환자 중 정신분열병 최초 발병 환자 혹은 최소 6개월 간 약물 치료를 하지 않은 정신분열병 환자 21명과 정상대조군 27명의 혈청 S100B 농도를 비교하였으며, 정신분열병 환자의 PANSS 전체점수와 양성증상점수, 음성 증상점수 등과 S100B의 혈청농도 간의 상관관계에 대해서 알아보았다. 결 과: 정신분열병 환자에서의 S100B 혈청농도($0.074{\pm}0.039$ ng/ml)와 정상대조군에서의 S100B 혈청 농도($0.072{\pm}0.030$ng/ml)에는 통계적인 차이는 없었다(p=0.925). 또한, 음성증상점수와 S100B 혈청농도(${\rho}$=0.410, p=0.065 ; 표 3)의 상관관계 및 양성증상점수와 S100B 혈청농도 (${\rho}$=-0.390, p=0.080 ; 표 3)의 상관관계는 통계적으로 유의하지 않았다. 결 론: 이번 연구에서 S100B 혈청농도와 정신분열병과의 관련성을 뚜렷하게 발견하기 어려웠다. 이러한 S100B의 역할과 연관성을 확인하기 위해서 연구대상의 확대와 CSF에서의 농도 측정, 장기적 추적 검사, 다른 관련 물질과의 연관성에 대한 추가적인 연구가 필요하다.

• 대한임상검사과학회지
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• 제49권2호
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• pp.128-134
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• 2017

Der p 1과 Der p 2는 알레르기 질환과 관련된 집먼지 진드기의 핵심적인 알러젠이다. 본 연구에서는 Der p 1과 Der p 2가 단구에서 S100A8과 S10A9을 분비시키는지를 확인하였고, 분비된 S100A8과 S10A9이 호중구의 세포고사 조절기전에 작용하는지를 연구하였다. Der p 1과 Der p 2는 정상인의 단구에서 S100A8과 S10A9을 유의하게 증가시켰고, S100A8과 S10A9은 정상인과 알레르기 질환 호중구의 자발적 세포고사를 억제 시켰다. 호중구의 Lyn, Akt, ERK는 S100A8과 S10A9을 시간별로 처리하였을 때 활성화하였다. 본 연구를 통하여 단구와 호중구에서 Der p 1과 Der p 2의 역할을 규명하였고, 나아가 관련된 알레르기 병인기전을 이해하는데 유용할 것이다.

• IMMUNE NETWORK
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• 제2권3호
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• pp.175-181
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• 2002

Background: S100A6 is a calcium-binding protein overexpressed in several tumor cell lines including melanoma with high metastatic activity and involved in various cellular processes such as cell division and differentiation. To detect S100A6 protein in patient' samples (ex, blood or tissue), it is essential to produce a monoclonal antibody specific to the protein. Methods: First, cDNA coding for ORF region of human S100A6 gene was amplified and cloned into the expression vector for GST fusion protein. We have produced recombinant S100A6 protein and subsequently, monoclonal antibodies to the protein. The specificity of anti-S100A6 monoclonal antibody was confirmed using recombinant S100A recombinant proteins of other S100A family (GST-S100A1, GST-S100A2 and GST-S100A4) and the cell lysates of several human cell lines. Also, to identify the specific recognition site of the monoclonal antibody, we have performed the immunoblot analysis with serially deleted S100A6 recombinant proteins. Results: GST-S100A6 recombinant protein was induced and purified. And then S100A6 protein excluding GST protein was obtained and monoclonal antibody to the protein was produced. Monoclonal antibody (K02C12-1; patent number, 330311) has no cross-reaction to several other S100 family proteins. It appears that anti-S100A6 monoclonal antibody reacts with the region containing the amino acid sequence from 46 to 61 of S100A6 protein. Conclusion: These data suggest that anti-S100A6 monoclonal antibody produced can be very useful in development of diagnostic system for S100A6 protein.

• IMMUNE NETWORK
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• 제3권1호
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• pp.16-22
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• 2003

Background: The S100A2 gene, also known as S100L or CaN19, encodes a protein comprised of 99-amino acids, is a member of the calcium-binding proteins of EF-hand family. According to a recent study, this gene was over-expressed in several early and malignant carcinomas compared to normal tissues. To elucidate the role of S100A2 protein in the process during carcinogenesis, production of monoclonal antibody specific to the protein is essential. Methods: First, cDNA sequence coding for ORF region of human S100A2 gene was amplified and cloned into an expression vector to produce GST fusion protein. Recombinant S100A2 protein and subsequently, monoclonal antibody to the protein were produced. The specificity of anti-S100A2 monoclonal antibody was confirmed by immunoblot analysis of cross reactivity to other recombinant proteins of S100A family (GST-S100A1, GST-S100A4 and GST-S100A6). To confirm the relation of S100A2 to cervical carcinogenesis, S100A2 protein in early cervical carcinoma tissue was immunostained using the monoclonal antibody. Results: GST-S100A2 recombinant protein was purified by affinity chromatography and then fusion protein was cleaved and S100A2 protein was isolated. The monoclonal antibody (KK0723; Korean patent pending #2001-30294) to the protein was produced and the antibody did not react with other members of EF-hand family proteins such as S100A1, S100A4 and S100A6. Conclusion: These data suggest that anti-S100A2 monoclonal antibody produced in this study can be very useful for the early detection of cervical carcinoma and elucidation of mechanism during the early cervical carcinogenesis.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2013년도 춘계학술대회
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• pp.317-318
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• 2013

국제수로기구(IHO)에서는 항해안전을 위한 다양한 수로데이터 사용을 지원하기 위해 범용수로데이터모델 S-100 표준을 간행한 바 있으며, 이러한 S-100표준은 레지스트리(등록소) 개념 도입을 통해 보다 다양한 수로분야의 데이터 관리 및 폭넓고 다양한 응용분야로의 적용이 가능하게 되었다. 본 연구에서는 우수하고 체계적인 S-100표준을 활용하고 국제수로분야에 기여하기 위해 해사안전분야에 관한 S-100기반의 제품표준을 시범적으로 개발하여 공통피쳐모델에 따른 응용스키마를 설계하였고 이를 간이용 레지스트리에 등록하여 피쳐 카탈로그를 생성하였다.

• 대한의생명과학회지
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• 제21권2호
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• pp.122-125
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• 2015

House dust mite (HDM) as a major allergen and damage-associated molecular pattern (DAMP) such as S100A8 and S100A9 trigger the pathogenesis and severity of allergic disease such as asthma. Regulation of neutrophil apoptosis is an important immune response and its dysregulation is involved in pathogenesis of allergic diseases. In this study, we examined the effects of HDM, S100A8 and S100A9 on spontaneous apoptosis of normal neutrophils. We considered the importance of the difference between in vitro and in vivo results and developed a new in vitro system consisting of a combination of immune cells and T helper (Th) cytokines. Extract of Dermatophagoides pteronyssinus (DP), S100A8, and S100A9 inhibited neutrophil apoptosis in culture of neutrophils alone without other leukocytes. DP and S100A8 more strongly suppressed neutrophil apoptosis in combinations of neutrophils, eosinophils, lymphocytes or monocytes than in a culture of neutrophils alone. Anti-apoptotic effect of S100A9 in the mixture of immune cells was similar to that in neutrophils. DP, S100A8, and S100A9 blocked neutrophil apoptosis, regardless of pretreatment with a T helper (Th) 1 cytokine (IFN-$\gamma$), Th2 cytokines (IL-4 and IL-10), a Th9 cytokine (IL-9), a Th17 cytokine (IL-17), a Treg-producing cytokine (TGF-$\beta$). These findings may enable elucidation of allergy pathogenesis due to HDM and DAMP.