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Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017

  • Seo, Hyun-Ji;Kim, Keun-Ho;Lee, Sang Kyu;Min, Subin;Lim, Ji-Yeon;Yang, Sun-Joo;Yoo, Mi-Sun;Jung, Sukchan;Yoon, Soon-Seek;Cho, Yun Sang
    • Korean Journal of Veterinary Research
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    • v.61 no.1
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    • pp.4.1-4.6
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    • 2021
  • Equine piroplasmosis (EP) is caused by Babesia caballi and Theileria equi infection. We investigated antigen and antibody of EP in horses in the Republic of Korea during 2016-2017. Antigen and antibody of T. equi was detected 0.06% (1/1,650). Phylogenetic analysis of 18S rRNA revealed that the T. equi was highly homologous with the strains from China, Mongolia, and Spain. Two Theileria spp. were also detected and highly homologous with T. buffeli, T. luwenshuni, and T. orientalis.

Heavy metals leaching behavior and ecological risks in water and wastewater treatment sludges

  • Wuana, Raymond A.;Eneji, Ishaq S.;Ugwu, Ezekiel C.
    • Advances in environmental research
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    • v.6 no.4
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    • pp.281-299
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    • 2017
  • Single (0.005 M DTPA), sequential (six-step) and kinetic (0.05 M EDTA) extractions were performed to assess Cd, Cr, Cu, Ni, Pb, and Zn mobilization and their potential ecological risks in Abuja (Nigeria) water (WTS) and wastewater (WWTS) treatment sludges. Total metal levels (mg/kg) in WTS and WWTS, respectively were: Cd(3.67 and 5.03), Cr(5.70 and 9.03), Cu(183.59 and 231.53), Ni(1.33 and 3.23), Pb(13.43 and 17.87), Zn(243.45 and 421.29). DTPA furnished metal extraction yields (%) in WTS and WWTS, respectively as: Cd(11 and 6), Cr (15 and 7), Cu(17 and 13), Ni(23 and 3), Pb(11 and 12), and Zn(37 and 33). The metals were associated with the soluble/exchangeable, carbonate, Mn/Fe-oxide, organic matter and residual forms to varying degrees. Kinetic extractions cumulatively leached metal concentrations akin to the mobilizable fractions extracted sequentially and the leaching data fitted well into the Elovich model. Metal mobilities were concordant for the three leaching procedures and varied in the order:WTS>WWTS. Calculated ecological risk indices suggested moderate and considerable metal toxicity in WTS and WWTS, respectively with Cd as the worst culprit. The findings may be useful in predicting heavy metals bioavailability and risks in the sludges to guide their disposal and use in land applications.

Analysis of Public and Researcher Interests in Suicide and Related Illnesses, and Acupuncture and Acupressure: Utilizing Google Trends and Major Electronic Database (자살 및 관련 질환과 침치료 및 혈위지압에 대한 대중과 연구자의 관심도 분석: Google Trends와 주요 전자 데이터베이스를 이용하여)

  • Sung-Hyun Kang;Jung-Gyung Lee;Chan-Young Kwon
    • Journal of Oriental Neuropsychiatry
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    • v.34 no.3
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    • pp.235-245
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    • 2023
  • Objectives: The aim of this study was to analyze public and researcher interests in suicide and related illnesses and acupuncture and acupressure treatment using Google Trends and some electronic databases. Methods: Search results for keywords "suicide," "acupuncture," "acupressure," and several illnesses related to suicide were analyzed in Google Trends from January 2004 to June 2023. Illnesses included anxiety, depression (including major depressive disorder), schizophrenia, bipolar disorder, post- traumatic stress disorder (PTSD), eating disorder (including anorexia nervosa and bulimia nervosa), substance use disorder, autism spectrum disorder, personality disorder (including borderline person- ality disorder), and chronic pain. Search results were extracted using relative search volume (RSV) scores between 0 and 100. Search terms were also searched in online databases, including PubMed, CNKI, and OASIS, to estimate the number of related studies, and descriptive analysis was conducted. Results: Google Trends analysis showed a strong positive correlation between the RSVs of "suicide and depression," "acupuncture and chronic pain," and "acupressure and PTSD." The electronic database search results produced numerous studies published on "suicide and depression," "acupuncture and depression," and "acupressure and anxiety." High interest in "suicide and depression," "acupuncture and chronic pain," and "acupressure and anxiety" was seen among the public and researchers. Interest in "suicide and chronic pain," "acupuncture and eating disorder," and "acupressure and PTSD" was higher in the public than among researchers, while "anxiety and suicide" and "anxiety and acu- puncture" showed opposite trends. Conclusions: The results of this research enable an understanding of public and researcher interest in suicide, acupuncture, acupressure, and suicide-related illnesses. The results also provide a basis for fu- ture research and examining public health implications in Korean medicine.

In vivo quantification of mandibular bone remodeling and vascular changes in a Wistar rat model: A novel HR-MRI and micro-CT fusion technique

  • Song, Dandan;Shujaat, Sohaib;Zhao, Ruiting;Huang, Yan;Shaheen, Eman;Van Dessel, Jeroen;Orhan, Kaan;Velde, Greetje Vande;Coropciuc, Ruxandra;Pauwels, Ruben;Politis, Constantinus;Jacobs, Reinhilde
    • Imaging Science in Dentistry
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    • v.50 no.3
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    • pp.199-208
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    • 2020
  • Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model. Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 ㎍/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared. Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat. Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization.

Study on Visible Diagnosis of Energy and Color (망기색에 대한 연구)

  • Kim, Yong-Chan;Kang, Jung-Soo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.4
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    • pp.840-847
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    • 2005
  • This study was written in order to help understanding of visible diagnosis of energy and color(기색). Visible diagnosis of energy and color is a very important factor of diagnosis and a necessary step of visible diagnosis. As human's body is under the control of spirit(신) and spirit(신) is stored by five viscera(오장), so spirit expresses the change of five viscera and is reflected by energy and color(기색). Strictly speaking, energy and color(기색) is not divided into each other. But, we can say that glittering glossy part belongs to energy(기) and blue, red, yellow, white and black part belongs to color(색). Various changes of energy and color(기색) observe the rules of yin-yang(음양). If we consider the interior and exterior(내외), top and bottom(상하), right and left(좌우), rise and fall(부침), clearness and muddiness(청탁), weak and severe(미심), spread and gather(산박), gloss and dness(택요) etc. we can decide the yin and yang(음양), inside and outside(표리), coldness and heatness(한열), truth and false(허보), life and death(생사) and prognosis. One man's own color is determined by the five human type(오형인), There are very various points of changing colors. As divided into principal groups, there are three main groups, that is, sky(천), earth(지) and man(인). A season(사시), day and night(주야) and cloud and clear(음청) belong to the factor of sky(천), a direction and configuration of the ground(지형) belong 治 the factor of earth(지), and motion and rest(동정), seven emotions(칠정), age and youth(노소), poor and rich(빈부) and high and low(귀천) belong to the factor of man(인).

A Change of fashin IIIustration by the Ideal Type of Human Body Beauty (인체미의 이상형에 따른 패션 일러스트레이션의 변화)

  • 전경숙
    • Journal of the Korean Society of Costume
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    • v.28
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    • pp.65-84
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    • 1996
  • The purpose of this study is to analyze the relation between the beauty of human body and the fashion illustration in each period And I attained my object through the investigation of features and changes of illustration which is given a vivid description of the features and development of ideal humam body beauty. By various methods I studied this subject. Frist I refered to sundry records Secondly I investiated the fashion illustrations which are included in Vogue. On the basis of this data I grasped the ideal types of human body beauty which is founded during the social change in each 10 years. And I analyzed the relation be-tween the ideal type and fashion illustration which show the change of fashion. The summary of result is as follows. 1. In the early part of the 20th century the beauty of human body is represented with the figure of large-sized beauty which emphasize shoulder and bust. And fashion ikllustration show 9 life-size broad shoulder full bust lim waist and hourglass silhouette. 2, In the 1910s the swell of strength vanishes gradually and shoulder and sleeve are straight type. And fashion illustration show 7 life-size high waist line. And that is tublar sil-houette of high waist and streamline shape in which bust and hip are not emphasized. 3. In the 1920s the ideal type of human body beauty is straight type which shows flat bust and unexaggerate hip. And fashion illus-tration is about 8 life-size tublar silhouette of low waist and lunger and slimmer and young style in which bust and hip are not emph-asized. 4. In the 1930s the ideal is womamly slim and long style. Fashion illustration is about 8 life-size and slim & long silhouette in which waist line is emphasized and bust and hip line come out. 5. In the 1940s the ideal type is womamly style which has narrow shoulder rich bast and slim waist. And fashion illustration is about 7 life-size and hourglass silhouette which has unartificial shoulder slim waist and empha-sized bust. 6. In the 1950s the ideal type is that of ro-bust health which emphasize build and muscu-lar system. And fashion illustration is 8.5 life-size and show full bust and made waist slimmer. That is sheath silhouette. 7. In the child who has full face with large eyeball slender and long leg: narrow and immatured body comparatively big head. And fashion illus-tration is 7 life-size and show slim and long neck flat bust long and slim limbs and big head. That is H type silhouette. 8. In the 1970s the ideal type is high stat-ure flat breast small hip and wide shoulders. And fashion illustration is wide shoulders and slim waist as 11 life-size and straight sil-houete. 9. In the 1980s the ideal type is extremely emphasized breadth of shoulder because healthy body and muscle are recognized as the symbol of ideal attractiveness. And fashion il-lustration is about 8.5 life-size and show mus-cular slim type that is slim silhouette. 10 At the present time the ideal type is slim and tall type which is empasized healthy beauty. And fashion illustration is 12 life-size which has healthy body and skin So that is slim and long type.

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Comparison of Pepper Anthracnose Pathogens from Korea and China

  • Shin, Hyun-Joo;Chen, Zhu-Jun;Hwang, Jae-Moon;Lee, Soon-Gu
    • The Plant Pathology Journal
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    • v.15 no.6
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    • pp.323-329
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    • 1999
  • Anthracnose of pepper casued by Colletotrichum spphas been a great problems for pepper production in Korea and China. Especially Colletotrichum gloeosporioides was found predominantly over cultivation areas during infection periods and caused severe rots on bath unripe and ripe fruits that resulted in major yield losses. In this study, comparison of Colletrichum spp.isolated from Korea and China in morphology and pathgenicity, and RAPD-PCR analysis were conducted. Based on morphological characteristics, the pathogen isolates, K1 and C1, K2 and C2, and K3 and C3 were identified as Colletotrichum gloeosporioides (G) type, C. gloeosporioides (R) type and C. coccodes, respectively. in pathogenicity test, K1 and C1, and K2 and C2 were found to attack mainly fruits and to be the most virulent among isolates. K3 and C3 were strongly virulent to leaves and seedling. Pathogenicity between Korean and Chinese isolates. K3 and C3 were strongly virulent to leaves and seedling. Pathogenicity between Korean and Chinese isolates did not show any difference. Results of the RAPD-PCR analyses indicate the varying levels of molecular diversity within and between Colletotrichum spp.of Korea and China. The similarities between K1 and C1, K2 and C2, and K3 and C3 were 85.71%, 71.43% and 50.0% respectively.

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Antioxidant activity and anti-inflammatory activity of ethanol extract and fractions of Doenjang in LPS-stimulated RAW 264.7 macrophages

  • Kwak, Chung Shil;Son, Dahee;Chung, Young-Shin;Kwon, Young Hye
    • Nutrition Research and Practice
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    • v.9 no.6
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    • pp.569-578
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    • 2015
  • BACKGROUND/OBJECTIVES: Fermentation can increase functional compounds in fermented soybean products, thereby improving antioxidant and/or anti-inflammatory activities. We investigated the changes in the contents of phenolics and isoflavones, antioxidant activity and anti-inflammatory activity of Doenjang during fermentation and aging. MATERIALS/METHODS: Doenjang was made by inoculating Aspergillus oryzae and Bacillus licheniformis in soybeans, fermenting and aging for 1, 3, 6, 8, and 12 months (D1, D3, D6, D8, and D12). Doenjang was extracted using ethanol, and sequentially fractioned by hexane, dichloromethane (DM), ethylacetate (EA), n-butanol, and water. The contents of total phenolics, flavonoids and isoflavones, 2,2-diphenyl-1 picryl hydrazyl (DPPH) radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured. Anti-inflammatory effects in terms of nitric oxide (NO), prostaglandin (PG) E2 and pro-inflammatory cytokine production and inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions were also measured using LPS-treated RAW 264.7 macrophages. RESULTS: Total phenolic and flavonoid contents showed a gradual increase during fermentation and 6 months of aging and were sustained thereafter. DPPH radical scavenging activity and FRAP were increased by fermentation. FRAP was further increased by aging, but DPPH radical scavenging activity was not. Total isoflavone and glycoside contents decreased during fermentation and the aging process, while aglycone content and its proportion increased up to 3 or 6 months of aging and then showed a slow decrease. DM and EA fractions of Doenjang showed much higher total phenolic and flavonoid contents, and DPPH radical scavenging activity than the others. At $100{\mu}g/mL$, DM and EA fractions of D12 showed strongly suppressed NO production to 55.6% and 52.5% of control, respectively, and PGE2 production to 25.0% and 28.3% of control with inhibition of iNOS or COX-2 protein expression in macrophages. CONCLUSIONS: Twelve month-aged Doenjang has potent antioxidant and anti-inflammatory activities with high levels of phenolics and isoflavone aglycones, and can be used as a beneficial food for human health.