• 한국유기농업학회지
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• 제5권1호
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• pp.87-99
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• 1996

Cultural conditions for mass production of the antagonistic bacteria, Bacillus subtills CAP134 against pathogens causing major airborne diseases to apple tree, effect of temperature, pH, carbon and nitrogen source in the culture broth were investigated. The bacterial growth was most vigorous when the temperature and pH of the culture broth was 30~$35^{\circ}$C, and 7, respectively. As for carbon source, dextrose was best followed in order by dextrose(monosaccharides)>sucrose(disaccharides)$\geq$saccharose(di-saccharides)>starch (polysaccharides). Among different sugars, bacterial growth was favored by in the order of brown, black and white sugars, indicating that the bacterial growth might be promoted by the minor elements presented as impurities in the less purified sugars. As for nitrogen source, organic forms were better to bacterial growth than inorganic forms, that is polypeptone was best followed in order by soy sauce, soybeen milk and inoganic nitrogens. Differences in bacterial growth among different forms of inorganic nitrogen were negligible.

• 한국유기농업학회지
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• 제6권1호
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• pp.85-98
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• 1997

Cultural conditions for mass production of the antagonistic bacteria, Bacillus subtills CAP141 against pathogens causing major airborne diseases to apple trees, effects of temperature, pH, carbon and nitrogen source in the culture broth were investigated. The bacterial growth was most vigorous when the temperature and pH of the culture broth was 30~35$^{\circ}$C and 7, respectively. As for carbon source, dextrose was best followed in order by dextrose(monosaccharide) > sucrose(disaccharide) $\geq$ saccharose (market disaccharides) > starch (polysaccharieds). Among different sugars, bacterial growth was favored by in the order of brown, black and white supars, indicating that the bacterial growth might be promoted by the minor elements presented as impurities in the less purified sugars. As for nitrogen source, organic forms were better to bacterial growth than inorganic forms, that is polypeptone was best followed in order by soy sauce, soybeen milk and inorganic nitrogens. Differences in bacterial growth among different forms of inorganic nitrogen were negligible.

• 식물병연구
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• 제14권1호
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• pp.43-50
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• 2008

Rhizoctonia solani AG2-2에 의해 발생되는 잔디 갈색퍼짐병(large patch)은 한국잔디로 식재된 골프장 페어웨이에서 문제가 되는 대표적 병해이다. 본 연구에서는 잔디 갈색퍼짐병의 생물학적 방제법에 이용할 수 있는 길항 미생물을 R. solani AG2-2에 대한 항균활성과 균사생장 억제율 등을 기준으로 1차 선발하였고, 선발된 길항미생물에 대해 폿트 수준의 생물검정실험을 실시하였다. 두 실험을 통해 선발된 길항미생물 중에서 상대적 길항효과 지수(RPI) 값을 기준으로 잔디 갈색퍼짐병에 대해 가장 우수한 것으로 최종 선발된 길항미생물 CJ-9는 동정한 결과 Bacillus subtilis로 밝혀져, B. subtilis CJ-9이라 명명하였다. B. subtilis CJ-9는 합성농약에 대한 내성 실험을 통해 현장에 적용시 화학 농약과의 혼용이 가능함을 밝혔다. 골프장에서 야외 포장시험을 실시한 결과, 실험 기간동안 합성농약에 의한 관행처리구에서 잔디 갈색퍼짐병이 2차례 발병된 것과 비교하여 B. subtilis CJ-9를 처리한 시험구에서는 전혀 발생하지 않아 방제 효과가 인정되었다. 선택배지를 이용한 병원균 밀도 조사를 통하여 B. subtilis CJ-9 처리가 잔디 갈색퍼짐병을 일으키는 병원균의 밀도를 감소시키는 것을 확인하였다. 따라서 B. subtilis CJ-9이 잔디 갈색퍼짐병 방제를 위해 합성농약을 대체할 수 있는 친환경적인 방제방법이 될 수 있음을 입증하였다.