• Journal of Veterinary Clinics
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• v.31 no.3
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• pp.206-211
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• 2014

Anaplasmosis is a rickettsial zoonosis mediated by blood-sucking arthropods, such as ticks, flies, and mosquitos. Migratory birds are common hosts of ticks that are mediators of anaplasmosis, in particular, the tick infection rate in thrushes (family Turdidae) has been known to be high. The main purpose of this study is to survey the occurrence and prevalence of Anaplasma spp. from the migratory thrushes in Jeju island. We collected blood samples from 6 thrushes rescued at the Jeju Wildlife Rescue Center and from 34 wild-caught thrushes on Mara island which is a satellite island of Jeju. As a result, the nested PCR confirmed that seven out of 40 individuals (17.5%) were infected by Anaplasma spp. and all of them were identified as A. phagocytophilum based on sequences obtained from partial 16S rRNA. All the infected birds were on their northward migration in spring, our results suggest that the Turdidae family, which is a common and abundant migrant group passing through Jeju island, may act a role as active reservoir and disperser of A. phagocytophilum causing potential influx of the zoonotic pathogens from its wintering grounds in lower latitude to the mainland Korea as well as Jeju.

• Korean Journal of Veterinary Research
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• v.34 no.2
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• pp.381-386
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• 1994

Evidence is presented for the isolation and characterization of Anaplasma avis in both indigenous (Korean) and exotic goats imported from Australia. These studies were carried out in response to epidemic scenario whereby farmers reported noticing what was described as a mysterious disease characterized by anemia, anorexia, general malaise and a significant morbidity and mortality rate in both types of goat breeds. The syndrome consistent with caprine anaplasmosis was associated with an intraerythrocytic agent occuring in single initial bodies characteristically surrounded by a single-layered membrane whereas the marginal body was typically surrounded by a double-layered membrane. The identity of the etiologic agent was confirmed as Anaplasma avis by light and ultrastructural microscopy.

• Parasites, Hosts and Diseases
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• v.56 no.6
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• pp.559-565
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• 2018

The identification and characterization of pathogenic and zoonotic tick-borne diseases like granulocytic anaplasmosis are essential for developing effective control programs. The differential diagnosis of pathogenic Anaplasma phagocytophilum and non-pathogenic A. phagocytophilum-like Anaplasma spp. is important for implementing effective treatment from control programs. The objective of the present study was to investigate the prevalence of Anaplasma spp. in horses in Korea by nucleotide sequencing and restriction enzyme fragment length polymorphism assay. Of the 627 horses included in the study, only 1 (0.2%) was infected with A. phagocytophilum. Co-infection with A. phagocytophilumlike Anaplasma spp. was not detected in the study. The 16S rRNA sequence of A. phagocytophilum was similar (99.5-100%) to A. phagocytophilum 16S rRNA isolated from horses in other countries. PCR adapted to amplify A. phagocytophilum groEL and msp2 genes failed to generate amplicons, suggesting genetic diversity in these genes. This study is the first molecular detection of A. phagocytophilum in horses in Korea. Human granulocytic anaplasmosis and animal infection of A. phagocytophilum have been reported in Korea recently. Because of vector tick distribution, global warming, and the increase of the horse industry, horses should be considered as a potential reservoir for A. phagocytophilum, and cross infectivity should be evaluated even though a low prevalence of infection was detected in this study. Furthermore, continuous surveillance and effective control measures for A. phagocytophilum should be established to prevent disease distribution and possible transmission to humans.

• Parasites, Hosts and Diseases
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• v.52 no.5
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• pp.507-511
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• 2014

Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil.

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.911-916
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• 1997

We observed an outbreak of calf anaplasmosis at a farm in Chonbuk area during winter season, which was diagnosed by the hematological and serological tests. The results are as follow. On hematological observation for infected thirteen calves (ages 1 to 25 days) showed anemia with hematocrit $27.7{\pm}7.7%$, erythrocyte $6.9{\pm}1.9{\times}10^6/{\mu}l$, hemoglobin $11.3{\pm}3.2g/dl$, MCV $40.6{\pm}1.5fl$, MCH $16.3{\pm}1.6pg$ and MCHC $40.5{\pm}3.6g/dl$. Anaplasma marginale was observed in all of the calves's erythrocytes by Diff-Quick and acridine orange staining, and were reacted by ELISA.

• Parasites, Hosts and Diseases
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• v.56 no.5
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• pp.521-525
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• 2018

Rodents are well-known reservoirs and vectors of many emerging and re-emerging infectious diseases, but little is known about their role in zoonotic disease transmission in Bhutan. In this study, a cross-sectional investigation of zoonotic disease pathogens in rodents was performed in Chukha district, Bhutan, where a high incidence of scrub typhus and cases of acute undifferentiated febrile illness had been reported in people during the preceding 4-6 months. Twelve rodents were trapped alive using wire-mesh traps. Following euthanasia, liver and kidney tissues were removed and tested using PCR for Orientia tsutsugamushi and other bacterial and rickettsial pathogens causing bartonellosis, borreliosis, human monocytic ehrlichiosis, human granulocytic anaplasmosis, leptospirosis, and rickettsiosis. A phylogenetic analysis was performed on all rodent species captured and pathogens detected. Four out of the 12 rodents (33.3%) tested positive by PCR for zoonotic pathogens. Anaplasma phagocytophilum, Bartonella grahamii, and B. queenslandensis were identified for the first time in Bhutan. Leptospira interrogans was also detected for the first time from rodents in Bhutan. The findings demonstrate the presence of these zoonotic pathogens in rodents in Bhutan, which may pose a risk of disease transmission to humans.