• Animal Systematics, Evolution and Diversity
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• v.40 no.4
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• pp.323-331
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• 2024

This study focuses on the diversity of ciliates inhabiting the oxygen-depleted conditions in freshwater ponds in Korea. We identified two species, Bothrostoma nasutum (Cunha, 1915) Jankowski, 1964 and B. undulans Stokes, 1887, for the first time in Korea. Their morphological characteristics were determined based on observations of both live and protargol-impregnated specimens. Bothrostoma nasutum is characterized by body size 70-85×25-35 ㎛ in vivo, body shape elongated ellipsoidal, anterior end extended as a proboscis; total 22-29 somatic kineties (including 7-8 postoral kineties), cortical granules arranged in 5 or 6 short, oblique rows; and 19-23 adoral membranelles. While B. undulans has the following features: body size 70-120×20-45 ㎛ in vivo, body shape elongated ellipsoidal, anterior end terminated by an acute snout; total 18-22 somatic kineties (including 5-7 postoral kineties); 17-22 adoral membranelles; and cortical granules obliquely arranged in 6 or 7 short rows. Recently, the increasing interest in research on anaerobic ciliates has contributed to the understanding of the diversity of ciliates in Korea.

• Animal Systematics, Evolution and Diversity
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• v.40 no.3
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• pp.211-220
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• 2024

During the surveys of ciliates from hypoxic habitats, three marine anaerobic species were found: Metopus spiculatus, M. vestitus, and Muranothrix felix. These species have not been previously recorded in South Korea and belong to the taxonomic classes Armophorea and Muranotrichea. The morphology of these species was examined by both microscopic observations of live specimens, and stained cells using protargol impregnations. Metopus spiculatus has the following characteristics: body size 80-110×25-35 ㎛ in vivo, beak-like structure at the end of preoral dome, ectosymbiotic bacteria covering cell surface, intracytoplasmic needle-shaped structures and the conspicuous tail end. Metopus vestitus has the following distinguishing characteristics: body size 95-130×25-45 ㎛ in vivo, a cone-shaped body, a covering of ectosymbiotic bacteria on its cell surface, intracytoplasmic needleshaped structures, somatic kineties arranged in 26-28 longitudinal rows, and a posterior part tapered into a tail. Muranothrix felix has the following characteristics: body size 100-130×20-30 ㎛ in vivo, elongated body with twisted neck region, bristle-like cilia protruding perpendicular to the cell margin, ectosymbiotic bacteria covering the cell surface, about 10 macronuclear nodules, and a long, stiffened caudal cilium.

• Korean Journal of Veterinary Research
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• v.35 no.2
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• pp.327-336
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• 1995

This study was carried out to develop the animal protein(feed for fry) that was isolated, purified and lyophilized the rumen ciliates from the healthy rumen contents which have $10^5-10^6/g$ ciliates and were discarded in abattoirs. The rumen ciliates are non-pathogenic, anaerobic and the weight of this protozoa is 2% of rumen content. The rumen protozoan and bacterial proteins both have a biological value for rats of 80-81, which is higher than the 72 of brewer's yeasts. Furthermore, the true digestibility and net protein utility of the protozoan protein are 91 and 73, much higher than those of bacterial(74 and 60) or yeast(84 and 60) proteins. The amino acids of rumen protozoa is nutritionally superior than the others. The size of rumen ciliates is $30-200{\times}20-110{\mu}m$ and so we had isolated and purified the rumen ciliates from the rumen contents by the physical methods. The purified rumen protozoa was lyophilized with freezing dryer. The results of this experiment were as follows : 1. Population dynamics of protozoan ciliates in slaughtered rumens; % of samples which small ciliates were predominated was 82.5%(52/63) and that of large ciliates was 17.5%(11/63). 1) predominant species of small ciliates were Entodinium ovinum and E nanellum. 2) predominant species of large ciliates were Epidinium ecaudatum and Diploplastron affine. 2. The lyophilized rumen ciliates which were isolated and purified from 1 kg of rumen content at the pH 6.2-6.8 was about 7.0 gram. 3. The nutrient analysis of lyophilized rqmen ciliates(LRC) was as follows: 1) Proximate analysis of the LRC and the composition of fry feed; moisture 8.05%(below 10.0), protein 35.37%(45), fat 5.39%(4.5), fiber 1.23%(below 2.5), ash 2.25%(below 15.0), Ca 0.26%(below 2.0), P 0.14%(below 1.1), energy 4,608.11(fish meal 5000 cal/g) 2) Amino acids (% in crude protein) of the LRC and the rotifer(Brachionus plicatilis); Arg 5.19%(4.50), His 2.50%(1.55), Ile 5.29%(3.45), Leu 8.11%(5.85), Lys 10.34%(6.15), Met 2.25% (0.85), Phe 5.66%(3.80), Thr 5.14% (3.45), Val 4.18%(3.90), Ala 4.13%(3.35), Asp 13.26%(8.25), Glu 16.62%(9.20), Gly 4.23%(3.10), Pro 3.25%(5.05), Ser 4.85%(3.85), Tyr 5.04%(3.05) 3) Fatty acids(% in fat) of the LRC and the rotifer(biological feed ; Brachionus plicatilis); myristic acid(C14:0) 3.27%(0.3), myristoleic acid(C14:1) 0.83%(-), palmitic acid(C16:0) 39.11% (23.5), palmitoleic acid(C16:1) 2.81%(2.0), stearic acid(C18:0) 9.36%(5.6), oleic acid(C18:1) 25.54%(3.5), linoleic acid(C18:2) 15.05%(32.9), linolenic acid(C18:3) 1.74%(9.8). Judging from the above investigated results, the analytical data of proximate analysis, amino acids, fatty acids of the purified and lyophilized rumen protozoa are reasonable for the feed of freshwater fishes(fry and fingerling). But it was disappointed of our expectation that the crude protein of lyophilized rumen ciliates contains low percentage, it was thought that because of the small ciliates(starch digester) in beef cattle rumens which were administered the concentrated feed, is much difficult to isolate and purify than the large ciliates(fiber digester).

• Journal of Species Research
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• v.11 no.2
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• pp.108-116
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• 2022

During a field survey to report unrecorded Korean ciliates, we collected five anaerobic ciliate species in two samples from freshwater and marine environments. These species belong to the classes Armophorea and Plagiopylea. The morphology of these species was studied based on live observations, protargol impregnation, and scanning electron microscope. Brachonella contorta is characterized by a size of 80-100×40-60 ㎛ in vivo, a bullet-shaped body, and about 60 oral polykinetids making 360° spiral around long axis. Brachonella pulchra is characterized by a size of 80-110×50-70 ㎛ in vivo and adoral zone composed of about 40 oral polykinetids with minimal spiralization. Metopus setosus is characterized by a size of 45-70×20-30 ㎛ in vivo, about 22 ciliary rows, very long caudal cilia, and 20 oral polykinetids. Plagiopyla nasuta is a freshwater species characterized by a size of 80-120×50-60 ㎛ in vivo, striated band present, straight extrusomes, and about 70 somatic ciliary rows. The marine Plagiopyla frontata is characterized by a size of 80-120×40-60 ㎛ in vivo, an oval to ellipsoid body shape, 50-60 somatic ciliary rows, and curved extrusomes.

• Microbiology and Biotechnology Letters
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• v.31 no.3
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• pp.264-270
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• 2003

A quantitative survey of the protozoa microfauna at bioreactor of advanced sewage treatment plant was carried out during a period of 11 months. In this study, 32 genera were identified, including 17 ciliates. The abundance of the important protozoa were compared with the operating parameters and water quality of the effluent of the plant using statistical procedure. Statistical analysis revealed a relationship between the abundance of some genera and removal rate. In particular, correlation analysis on the quality of effluent and protozoa indicated that Lepadella may be used as the bioindicator of TP removal and Trochilia, Entosiphon, Colepus may be used as the bioindicator of TN removal when water temperature was below $20^{\circ}C$.