• 한국식품영양과학회지
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• 제23권4호
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• pp.614-617
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• 1994

단백질이 풍부한 볶은 콩가루를 달리 첨가(0, 5, 10, 15, 20%)하여 절편의 아미노산 함량, 아밀로스 함량과 무기물 함량에 미치는 영향을 조사하였다. 콩가루를 첨가한 절편의 아미노산 함량을 조사한 결과 콩가루 첨가량의 증가에 따라 곡류의 제한 아미노산인 라이신의 함량이 증가하였다. 대조구는 259mg/100g인데 비하여 콩가루 첨가군은 320에서 625mg/100g 까지 1.28배에서 2.50배 까지 증가하였다. 또 필수아미노산 함량과 총 아미노산 함량도 대조구에 비하여 1.22배에서 2.04로 증가하였다. 콩가루 첨가량이 증가함에 따라 절편의 노화를 일으키는 아밀로스 대조구에 비하여 0.9~4.7%(w/w) 정도 감소함을 알 수 있었다. 절편의 다량 무기물 함량은 콩가루 첨가에 따라 증가하였고 철분, 아연, 구리, 망간 등의 미량 무기물의 함량도 콩가루 첨가량의 증가에 따라 비례적으로 증가하는 경향이었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.273-273
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• 2022

The increasing number of Westernized eating patterns based on wheat flour in Korea has led to an increase in the rate of diseases such as obesity and diabetes, which has become a social problem. Wheat consumption is increasing due to changes in eating habits, but domestic wheat has low price competitiveness and has stagnated recently, so it is necessary to secure new resources to differentiate from imported wheat. Resistant starch, a newly created resource in domestic wheat, can act as a prebiotic similar to dietary fiber in the body, inducing microbial changes in the gut and having beneficial effects on metabolic syndrome. Wheat research on resistant starch was carried out based on the breeding of high amylose. A genome-wide association study (GWAS) was used to perform SNP identification and expression analysis related to wheat amylose through phenotype and genotype. 561 wheat core collection gene sources were investigated for amylose content in wheat, and related genes were extracted and analyzed. In the GWAS analysis, the model formulas BLIMK, FarmCPU, GLM, MLM, and MLMM were used to derive results such as QQ plots and Manhattan plots through phenotypic data. Among these models, BLAST was conducted to find the association between the SNPs identified using FarmCPU and genes related to starch, and 15 were found. Using the identified markers, it becomes easier to develop and browse related wheat cultivars according to their amylose content.

• Journal of Crop Science and Biotechnology
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• 제11권3호
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• pp.181-186
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• 2008

To assess the potential as biofortified rice varieties, new endosperm and grain mutant lines were selected from $M_4$ generation seeds of the rice cultivar Shindongjin, which were either $\gamma$-irradiated or treated with N-methyl-N-nitrosourea(MNU) and lipid, sugar, and tocopherol content were analyzed. Amylose content in non-waxy mutants ranged from 8.8% in SM-4, a dull-type mutant, to 29.5% in SM-51, compared to 18.9% in the parental variety, Shindongjin. SM-23, a floury-type mutant, contained 0.09 ${\mu}g/g$ $\alpha$-tocopherol(40.9% of total tocopherol), was three times higher than in the parental variety. SM-32, a giant embryo-type mutant, had a 2.2-fold higher total tocopherol content, 2.1-fold higher $\alpha$-tocopherol, and 5.5-fold higher $\delta$-tocopherol content(47.3% of total tocopherol) than the parental variety(0.13 ${\mu}g/g$). Total free sugar content was elevated in all selected mutants and 1.2-8.6 times higher than in the parental variety(11.38 ${\mu}g/g$). These increased sugar levels were due to increase in sucrose concentration. SM-23(floury-type mutant) and SM-51(high amylose-type mutant) had 4.6 and 7.0 times more sugar, respectively, than the parental variety(11.38 ${\mu}g/g$). With relatively high concentrations, most mutants showed elevated fatty acid content in the SM 32(giant embryo-type) and SM-51(high amylose-type) mutants, at 124.56 and 89.59 mg/g, respectively. All selected mutants displayed valuable characteristics for the development of new varieties in rice-breeding programs.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.295-295
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• 2017

Rice (Oryza sativa L.) is one of the most important food crops in the world. The eating quality of cooked rice is the most important trait japonica rice breeding in Korea. Rice varieties that produce kernels that are firm and fluffy after cooking are generally favored in countries such as India, Pakistan, and Indonesia. Whereas varieties with kernels that maintain its shape, glossiness, savory odor, stickiness, and tenderness when cooked are preferred in Korea. This study analyzed the major physicochemical components of rice grain associated with the eating quality of 20 japonica rice varieties. Physicochemical components such as the amylose content, protein content, amylographic characteristics of polished rice, and texture of cooked rice were tested using a Tensipresser as alternative indirect methods in determining rice eating quality. Evaluation of eating quality of cooked rice using sensory test was conducted with 20 well trained members. The 20 rice varieties in different eating quality showed amylose contents of 17~20%. The amylose content of rice varieties had negative correlation with peak viscosity, however positive correlation with setback viscosity was observed. The stickiness and adhesiveness of cooked rice showed correlation with the amylose content and amylopectin chain length distribution. Rice varieties with good eating quality showed less retrogradation of cooked rice and higher hot viscosity of rice flour in amylogram.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.325-325
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• 2022

In order to developing high-quality rice varieties and processing varieties, systematic and stable evaluation of physicochemical properties is required for breeding lines. In this study, we compared the quality characteristics evaluation results of rice breeding lines for cooked rice, special rice, and whole crop silage rice adapted to central plain region (Suwon) in order to use as basic data for future rice variety development. Brown rice length/width ratio, head rice ratio, protein content, amylose content, alkali digestion value(ADV) and Toyo value were analyzed to evaluate the quality characteristics of yield trial lines cultivated in Suwon for four years (2017-2020). Brown rice length/width ratio, head rice ratio, protein content, ADV and Toyo values showed significant differences by year, but there was no significant difference in amylose content (p<0.05), which showed little environmental variation. The head rice ratio and Toyo value showed an increasing trend, while the protein content showed a decreasing trend. However, the protein content was the highest in 2020, which is thought to be owing to little sunlight hours due to heavy rainfall in 2020. The protein content of whole crop silage rice was 8.1%, which was significantly higher than that of other lines (p<0.05). Toyo value of medium-maturing and early-maturing lines were 67.6 and 73.7%, respectively, and the Toyo value of medium-maturing lines was higher than that of the early-maturing lines (p<0.05). In correlation analysis among the quality characteristics of the rice lines for cooked rice, significant positive correlations were detected between Toyo value and head rice ratio, amylose content, ADV, and a negative correlation was observed between Toyo value and protein content (p<0.05).

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.70-70
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• 2017

This study was conducted to characterize the amylose and protein contents of 4,948 rice landrace germplasm using the NIRS model developed in the previous study. The amylose contents estimated by NIRS in the standard rice were Sinseonchal (6.881%) 4.994%, Chucheong (19.731%) 18.633%, Goami (23.246%) 20.548%. Protein contents were Sinseonchal (6.890%) 6.824%, Chucheong (6.350%) 6.869%, Goami (6.777%) 7.839%. The NIRS analysis showed that 1.1-2.7%point lower in amylose and 0.4-0.6%point higher in protein than standard contents. The average amylose content of the germplasm was 20.39% with a range of 3.97-37.13%. The average protein content was 8.17% with a range of 5.20-17.45%. Amylose contents with a range of 20.06-27.02% represented 62.20% of the germplasm. Protein contents with a range of 6.78-9.75% represented 81.60% of the germplasm. Korean landrace comprised 24.9% among the 4,948 germplasm collected from 41 countries. A specific range of amylose contents showed in Korea 16.58-20.06%, in Japan 20.06-23.25%, in North Korea 23.25-27.02% and in China 27.02-37.13%. Protein contents exhibited 5.20-17.45% evenly in the whole landraces, whereas Chinese landrace particularly observed with 6.78-8.27% and 9.75-17.45%. Fifty resources were selected with low and high amylose ranging from 3.97-6.66% to 30.41-37.13% respectively. Similarly fifty resources were selected with low and high protein ranging from 5.20-6.09% to 13.21-17.45% respectively. Landraces with higher protein should be adapted to practical utilization of food sources.

• 한국식품영양과학회지
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• 제40권12호
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• pp.1797-1801
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• 2011

아밀로오스 함량에 차이가 있는 일반 멥쌀 현미(아밀로오스 20.1%)와 저아밀로오스 현미(아밀로오스 7.3%)를 제분 방법을 달리하여 현미가루로 제조한 후 제분방법별 현미가루의 이화학적 특성을 비교 조사하였다. Pin mill로 건식제분 한 현미가루의 입자크기가 수침후 roll mill에 의해 습식제분 한 현미가루에 비해 작았고 입자크기는 습식제분 하여 건조한 현미가루를 2차 건식 재분쇄하는(습식/건식) 제분방식에 의해 더 감소하였다. 현미가루의 전분손상도는 건식제분 현미가루가 14.6~15.6%로 습식제분 4.2~4.8%에 비해 현저하게 높았으나 습식/건식제분이 그대로 건식제분 하는 방법에 비해 입자크기가 미세해지면서 현미가루의 전분손상도는 줄여줄 수 있는 것으로 나타났다. 현미가루의 수분흡수지수는 습식/건식 현미가루에서 가장 낮았으며 수분용해도지수는 건식>습식/건식>습식제분 순이었다. 습식제분 한 현미가루가 건식제분보다 최고점도가 낮은 반면에 paste의 최종점도는 증가하여 setback은 증가하는 경향을 보였다. 제분방법별 일반 현미가루의 평균 최고점도, trough, breakdown, 최종점도, setback이 저아밀로오스 현미가루에 비해 훨씬 높게 나타났으며 전분의 노화 정도에 서로 차이가 있을 것으로 예측되었다.

• 한국식품조리과학회지
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• 제8권3호
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• pp.255-263
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• 1992

Pasting characteristics and amylose-surfactant complex forming ability of sweet potato starch were investigated after defatting and the addition of surfactants, such as SSL (sodium steamyl-2-lactylate) Dimodan (mono/di glyceride) and SE (sucrose ester) with different concentrations. All starch granules were smooth and round, there were no damages to starch granules after defatting and surfactant addition. amylose content of surfactant added stach decreased and me order of decrease was SSL, SE and Dimodan. The cornplex forming ability of SE added starch increased according to increasing HLB value. As surfactants concentration increased, amylose complex formig ability increased. In case of gelatinization patterns by amylograph, the initial pasting temperature of surfactant added starches was higher than mat of untreated or defatted starches, but viscosity at each temperature were all decreased. Soluble carbohydrate and leached amylose of starches increased at increasing temperature, those of surfactant added starches decreased at each temperature in the order of SSL, SE and Dimodan.

• 한국식품과학회지
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• 제29권2호
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• pp.383-386
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• 1997

아밀로오스 함량이 다른 옥수수 전분을 고압 가열-냉각 사이클을 4회까지 반복한 후, 효소 중량법과 ${\alpha}-amylase$ 처리로 효소저항전분을 분리하여 그 수율을 비교하였다. 분리방법에 상관없이 아밀로오스 함량이 높을수록 효소저항전분의 수율이 증가하였고, 가열-냉각 횟수를 증가시키면 효소저항전분의 수율도 증가하였다. 효소저항전분의 분리는 효소 중량법이 ${\alpha}-amylase$ 처리로 효소저항전분을 분리할 때보다 순도가 높았다.

• 한국작물학회지
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• 제44권3호
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• pp.277-281
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• 1999

Dull grains segregated from F$_3$ and F$_4$ of the crosses between two dull mutants and a glutinous cultivar were compared with non-glutinous and glutinous segregants for their physicochemical properties. Amylose content of dull rice grain segregated from the dull/glutinous cross showed the intermediate value between glutinous and non-glutinous rice grain, whether it is controlled by the recessive or dominant gene. Alkali digestibility value (ADV) of dull rice grain was lower than that of glutinous or non-glutinous rice. A positive correlation was found between ADV and amylose content of homozygous non-glutinous or dull F$_4$ grains, but a negative relationship was observed in glutinous grains. Protein content of dull grain was significantly higher than that of glutinous or non-glutinous grain segregated from the same cross, while those of glutinous and non-glutinous grains were not different. Among gelatinization characteristics, initial pasting temperature and peak viscosity of dull grains were higher than glutinous rice, and were not different with non-glutinous grain. Hot, cool and consistency viscosities of dull grain were intermediate between glutinous and non-glutinous rices. Dull grains showed the highest breakdown viscosity and the lowest setback viscosity among the three endosperm types.