• Journal of Dairy Science and Biotechnology
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• 제34권1호
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• pp.43-49
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• 2016

Emulsion PCR (ePCR) has recently gained interest in the areas of food safety and biotechnology owing to its highly specific and sensitive performance in the amplification of target DNA. To facilitate the applications of ePCR to food safety and biotechnology, this paper describes the principles of ePCR and the factors that should be considered in designing ePCR. In addition, current research and applications related to ePCR are discussed.

• 한국방재학회:학술대회논문집
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• 한국방재학회 2011년도 정기 학술발표대회
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• pp.12-19
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• 2011

Drawing on its extensive experience with natural disasters, Japan has been dispatching Japan Disaster Relief (JDR) team to disaster-stricken countries to provide specialist assistance in rescue and medical operations. The JDR team has assisted in the wake of disasters including the 2004 Indian Ocean Earthquake and the 2008 Sichuan Earthquake in China. Information about the affected area is essential for a rapid disaster response. However, it can be difficult to gather information on damages in the immediate post-disaster period. To help overcome this problem, we have built on an Earthquake Damage Estimation System. This system makes it possible to produce distributions of the earthquake's seismic intensity and structural damage based on pre-calculated data such as landform and site amplification factors for Peak Ground Velocity, which are estimated from a Digital Elevation Model, as well as population distribution. The estimation result can be shared with the JDR team and with other international organizations through communications satellite or the Internet, enabling more effective rapid relief operations.

• Structural Engineering and Mechanics
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• 제44권5호
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• pp.681-704
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• 2012

The dynamic response of Euler-Bernoulli beams to resonant harmonic moving loads is analysed. The non-dimensional form of the motion equation of a beam crossed by a moving harmonic load is solved through a perturbation technique based on a two-scale temporal expansion, which permits a straightforward interpretation of the analytical solution. The dynamic response is expressed through a harmonic function slowly modulated in time, and the maximum dynamic response is identified with the maximum of the slow-varying amplitude. In case of ideal Euler-Bernoulli beams with elastic rotational springs at the support points, starting from analytical expressions for eigenfunctions, closed form solutions for the time-history of the dynamic response and for its maximum value are provided. Two dynamic factors are discussed: the Dynamic Amplification Factor, function of the non-dimensional speed parameter and of the structural damping ratio, and the Transition Deamplification Factor, function of the sole ratio between the two non-dimensional parameters. The influence of the involved parameters on the dynamic amplification is discussed within a general framework. The proposed procedure appears effective also in assessing the maximum response of real bridges characterized by numerically-estimated mode shapes, without requiring burdensome step-by-step dynamic analyses.

• Advances in materials Research
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• 제6권2호
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• pp.155-168
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• 2017

The terrorist attacks and dangers by bomb blast have turned into an emerging issue throughout the world and the protection of the people and structures against terrorist acts depends on the prediction of the response of structures under blast and shock load. In this paper, behavior of aluminum and unidirectionally reinforced E-Glass Epoxy composite plates with and without focal circular holes subjected to shock loading has been identified. For isotropic and orthotropic plates (with and without holes) the classical normal mode approach has been utilized as a part of the processing of theoretical results. To obtain the accurate results, convergence of the results was considered and a number of modes were selected for plate with and without hole individually. Using a shock tube as a loading device, tests have been conducted to composite plates to verify the theoretical results. Moreover, peak dynamic strains, investigated by experiments are also compared with the theoretical values and deviation of the results are discussed accordingly. The strain-time histories are likewise indicated for a specific gauge area for aluminum and composite plates. Comparison of dynamic-amplification factors between the isotropic and the orthotropic plates with and without hole has been discussed.

• 한국정밀공학회지
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• 제31권3호
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• pp.277-283
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• 2014

This paper presents the design, optimization and fabrication of a piezo driven micro-positioning stage constructed using a 3D-printer. 3D printing technology provides many advantageous aspects in comparison to traditional manufacturing techniques allowing more rapid prototyping freedom in design, etc. Micro-positioning stages have traditionally been made using metal materials namely aluminum. This paper investigates the possibility of fabricating stages using ABS material with a 3D printer. CAE simulations show that equivalent motion amplification can be achieved compared to a traditional aluminum fabricated stage while the maximum stress is 30 times less. This leads to the possibility of stages with higher magnification factors and less load on the driving piezo element. Experiment results agree with the simulation results. A micro-position stage was fabricated using a 3D printer with ABS material. The motion amplification is very linear and 50 nm stepping was demonstrated.

• Earthquakes and Structures
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• 제17권1호
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• pp.75-89
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• 2019

The paper presents experimental and numerical studies carried out on low-rise RC frames, typically found in developing countries. Shake table tests were conducted on 1:3 reduced scaled two-story RC frames that included a code conforming SMRF model and another non-compliant model. The later was similar to the code conforming model, except, it was prepared in concrete having strength 33% lower than the design specified, which is commonly found in the region. The models were tested on shake table, through multiple excitations, using acceleration time history of 1994 Northridge earthquake, which was linearly scaled for multi-levels excitations in order to study the structures' damage mechanism and measure the structural response. A representative numerical model was prepared in finite element based program SeismoStruct, simulating the observed local damage mechanisms (bar-slip and joint shear hinging), for seismic analysis of RC frames having weaker beam-column joints. A suite of spectrum compatible acceleration records was obtained from PEER for incremental dynamic analysis of considered RC frames. The seismic performance of considered RC frames was quantified in terms of seismic response parameters (seismic response modification, overstrength and displacement amplification factors), for critical comparison.

• Geomechanics and Engineering
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• 제30권1호
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• pp.1-10
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• 2022

Seismic ground response evaluation is one of the main issues in geotechnical earthquake engineering. These analyses are subsequently divided into one-, two- and three-dimensional methods, and each of which can perform in time or frequency domain. In this study, a novel approach is proposed to assess the seismic site response using two-dimensional transfer functions in frequency domain analysis. Using the proposed formulation, a program is written in MATLAB environment and then promoted utilizing the equivalent linear approach. The accuracy of the written program is evaluated by comparing the obtained results with those of actual recorded data in the Gilroy region during Loma Prieta (1989) and Coyote Lake (1979) earthquakes. In order to precise comparison, acceleration time histories, Fourier amplitude spectra and acceleration response spectra diagrams of calculated and recorded data are presented. The proposed 2D transfer function diagrams are also obtained using mentioned earthquakes which show the amount of amplification or attenuation of the input motion at different frequencies while passing through the soil layer. The results of the proposed method confirm its accuracy and efficiency to evaluate ground motion during earthquakes using two-dimensional model. Then, studies on irregular topographies are carried out, and diagrams of amplification factors are shown.

• 한국항만학회지
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• 제6권2호
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• pp.43-64
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• 1992

This study discusses the interacting with deep water waves approaching from deep water based on the linear wave theory and steep sloping sea bottom floor by the numerical procedure. The results of particular interest are particle velocity and acceleration in x, y, z direction wave height amplification factor reflection coefficient and dimensionless pressure distribution on the steep sloping bottom with respect to the various incident wave angle. The wave loads relative to various bottom slopes, incident wave angles and wave periods on submerged breakwater and pipe are represented in comparison with mild sloping bottom the wave load parameters on the steep sloping bottom seemed to be influenced by variation of incident wave angle. In general the particle velocities and accelerations in x, y, z directions on the steep sloping bottom represented larger value or about two than those on the mild sloping bottom according to incident wave angle. However, the wave height amplification factors did not show distinct difference, but the slight variation with respect to the various incident angle showed on mild sloping bottom. The reflection coefficient increased with respect to increase of the incident angle on the steep sloping bottom the results also indicate that the very steep sloping beach produces a rather substantial amount of reflection as we expected. No significant variation of wave pressure was shown on the steep sloping bottom but it represented a certain amount of variation on the mild sloping bottom according to the various incident wave angle. The analysis at the OTEC site also showed similar results.

• Earthquakes and Structures
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• 제23권3호
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• pp.283-295
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• 2022

Seismic design codes permit the use of Equivalent Static Analysis of buildings considering torsional eccentricity e with dynamic amplification factors on structural eccentricity and some accidental eccentricity. Estimation of e in buildings is not addressed in codes. This paper presents a simple approximate method to estimate e in RC Moment Frame and RC Structural Wall buildings, which required no detailed structural analysis. The method is validated by 3D analysis (using commercial structural analysis software) of a spectrum of building. Results show that dynamic amplification factor should be applied on torsional eccentricity when performing Response Spectrum Analysis also. Also, irregular or mixed modes of oscillation arise in torsionally unsymmetrical buildings owing to poor geometric distribution of mass and stiffness in plan, which is captured by the mass participation ratio. These irregular modes can be avoided in buildings of any plan geometry by limiting the two critical parameters (normalised torsional eccentricity e/B and Natural Period Ratio 𝜏 =T_𝜃/T, where B is building lateral dimension, T_𝜃 uncoupled torsional natural period and T uncoupled translational natural period). Suggestions are made for new building code provisions.

• Environmental Analysis Health and Toxicology
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• 제11권1_2호
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• pp.1-10
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• 1996

A decrease in stratospheric ozone probably caused by chloroflurocarbons (CFCs) emissions, has been observed large parts of-the globe. It is generally accepted that if ozone levels in the stratosphere are depleted, greater amounts of shortwave ultraviolet radiationB (UVB) will reach the earth's surface, resulting in increased incidence of nonmelanoma skin cancer. In this study, we evaluated several mathematical models, such as a power and an exponential model, and a geometric model considering the surface area of a human body part and ages for the prediction of Skin cancer incidence caused by exposure to the UVB radiation. These models basically estimated the risk of skin cancer based on those measurements of the local ozone in stratosphere and UVB. Both were measured at a part of Seoul with a Dobson ozone spectrometer and Robertson-Berger UV Biometer for 1995. As a result, we calculated the point estimation applying a biological amplification factor (BAF), UVB radiation and other factors. We used a Monte-Carlo simulation technique with assumption on the distribution of each considered factor. The sensitivity analysis of model by there components conducted using Gaussian sensitivity method. The annual integral of UVB radiation was 2275 MED (minimal erythema dose)/yr. Also, an estimate of the annual amount of UVB reaching the earth's surface at a korea's latitude and altitude was 3328 MED/yr. The values of the radiation amplification factor (RAF) were ranged from 0.9 to 1.5 in Seoul. To give the effective factors required to model the prediction of skin cancer incidence caused by exposure to the UVB radiation in Korea, we studied the pros and cons of above mentioned models with the application of those parameters measured in Seoul, Korea.