• Title/Summary/Keyword: altemansucrase

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Modification of Acetobacter xylinum Bacterial Cellulose Using Dextransucrase and Alternansucrase

  • Kim, Do-Man;Kim, Young-Min;Park, Mi-Ran;Park, Don-Hee
    • Journal of Microbiology and Biotechnology
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    • v.9 no.6
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    • pp.704-708
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    • 1999
  • In addition to catalyzing the synthesis of glucan from sucrose as a primary reaction, glucansucrase also catalyzes the transfer of glucose from sucrose to other carbohydrates that are present or are added to the reaction digest. Using dextransucrase and altemansucrase, prepared from Leuconostoc mesenteroides B-742CBM and B-1355C, respectively, we modified the bacterial cellulose in Acetobacter xylinum ATCC10821 culture, and then produced a characteristic cellulose that is soluble and has a new structure. There were also some partially modified insoluble cellulose and oligosaccharides in the modification culture. After methylation and following acid hydrolysis of both the soluble and insoluble glucans, there were ($1{\rightarrow}4$) as well as ($1{\rightarrow}6$) and ($1{\rightarrow}3$) glycosidic linkages in the soluble glucan.

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