• Research in Plant Disease
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• v.11 no.1
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• pp.43-47
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• 2005

To investigate control efficacy of flusulfamide GR (granule) on Chinese cabbage clubroot caused by Plasmodiophora brassicae, experiment was accomplished in field located in Gangneungshi alpine area contaminated by P. brassicae. Flusulfamide GR provided control value of 84.6% and that was statistically significant difference from standard fungicides containing untreated control. To investigate ratio of reduction of resting spore according to fungicide treatment, soil of Chinese cabbage field before and after fungicide treatment were sampled and investigated density of resting spore. Resting spore density was not uniform in soil before fungicide treatment. Therefore, to investigate control efficacy of fungicide against clubroot, investigation on resting spore density was conducted before experiment and reflected in experimental design. Flusulfamide GR and DP (dust powder) provided 64.2% and 63.7% of reduction of resting spore on field soil after fungicide treatments. This result indicated that control efficacy of the fungicides was correlated with reduction of resting spore of P. brassicae. The increasing rate in fresh weight of above-ground part of Chinese cabbage by flusulfamide DP and GR, fluazinam DP and trifloxystrobin SC (suspension concentrate) was 14.3%, 13.0%, 13.8% and 3.8%, respectively. From above result, flusulmide GR have outstanding control efficacy against clubroot of Chinese cabbage and is effectively decreasing of resting spore density in soil.

• The Plant Pathology Journal
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• v.22 no.1
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• pp.97-102
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• 2006

A virus causing vein banding, sometimes yellow mosaic and rugose symptoms on peanut was prevalent around Suwon, Korea. A survey conducted in the area found disease incidence, depending on cultivar, to range from 79 to $100\%$. The virus was found to be seed-transmissible in all the five peanut cultivars tested with transmission rates ranging from 2 to $16\%$. Host range analysis failed to differentiate 9 field isolates collected from different peanuts cultivars showing various symptoms. Inclusion bodies such as scroll, pinwheel and long laminated aggregates induced by the virus in host plant cells were similar to those induced by members of the Potyvirus subdivision III. The virus showed < $95\%$ homology with Bean common mosaic virus (BCMV), BCMV-BICMV/AzMV strains and only < $91\%$ with Desmodium mosaic virus. Based on biological characterization, electron microscopy and molecular analyses of a Korean isolate (Daewon 1), the virus was identified as peanut stripe strain of BCMV.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.381-381
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• 2011

본 연구는 고랭지 밭 지역에서 발생하는 강우유출수의 특성을 알아보기 위하여 2009년 7월부터 10월까지 유량과 수질농도를 분석하였다. 모니터링 지점은 소양강 댐 상류 지점인 홍천군 자운지구의 3개 소유역이며, 하천 주변에 농경지가 집중적으로 발달하여 강우시 농경지로부터 발생하는 비점오염의 영향이 클 것으로 판단하였다. 모니터링 결과는 기저유출 부하를 산정하기 위해 N-Day법을 이용하여 직접/기저 유출수로 분리하고, 각 유출수의 오염부하를 산정하여 비교하였다. 기저오염 부하를 산정하기 위해 평시모니터링을 실시하였으며, 3개 소유역에서 조사된 평시 수질항목의 농도는 큰 차이를 보이지는 않았다. 1차와 2차 강우사상에서는 3개의 소유역에서 수질항목의 직접유출 부하가 80% 이상을 차지하는 것으로 나타났다. 또한, 전체 강우사상에서 SS와 TP의 직접 오염부하가 다른 수질항목에 비해 크게 나타났으며, TN의 경우 다른 항목에 비해 기저유출 부하가 큰 것으로 조사되었다. 또한, 100 mm 이상의 강우시에는 대부분의 항목이 직접유출 부하가 더 큰 것으로 조사되었으나, 6차와 7차의 적은 강우시에는 SS와 TP를 제외한 항목에서 기저유출 부하가 큰 것으로 조사되었다. SS는 탁도를 유발시키는 주요 원인이 되며, TN과 TP는 부영양화의 원인이 된다. 또한, 강우시 오염부하는 직접유출 뿐만 아니라 기저유출의 영향도 큰 것으로 나타났으며, 기저유출의 관리가 어렵기 때문에 하천으로 유입되는 직접유출을 우선적으로 관리할 필요가 있다. 따라서 연구 지역과 유사한 지역의 수질 관리를 위해서는 직접유출 형태로 발생하는 SS와 TP를 줄이기 위한 비점오염저감시설 또는 최적관리방법의 도입이 필요한 것으로 판단된다.

• The Plant Pathology Journal
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• v.18 no.3
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• pp.130-137
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• 2002

Potato virus Y (PVY) is one of the most important viruses in many field crops in Korea. In this study, 31 PVY isolates were isolated from infected potato (Solanum tuberosum), tobacco (Nicotiana tabacum), pea (Pisum sativum), and weeds (Veronica persica, Lamium amplexicause and Capsella bursa-pastoris) showing different mosaic symptoms in Jeonbuk, Chungnam, Gangwon, and Gyeongbuk areas in Korea. The 640 nucleotide region containing the C-terminal portion of coat protein (CP) gene and 3'non-translated region (NTR) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using PVY-specific oligonucleotide primers. Sequence analyses of the amplified DNA fragments showed that the C-terminal portion of CP gene was not significantly different from that of previously reported PVY strains from potato (PVY-OK and -T) and tobacco (PVY-VN) in Korea. Homologies of the deduced CP amino acid sequences were 93.3-99.0% to corresponding regions of the other PVY strains including PV $Y^{N}$, PV $Y^{o}$ , PV $Y^{OK}$ , PV $Y^{T}$ , and PV $Y^{VN}$ . In contrast the sequences located at the 3'-NTR showed more diverse sequence homologies (76.4-99.7%). These results indicate that the C-terminal portion of the CP gene was relatively conserved while sequences at the 3'NTR were more diverse and variable over the host species and the regions where they were isolated.e isolated.

• Research in Plant Disease
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• v.16 no.2
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• pp.125-128
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• 2010

Bacterial black spot disease of prune fruit (Prunus salicina cv. formosa) has outbroke around major prune production area, Gimcheon, Euiseong and Gunwi in Gyungbuk province and has caused severe economic loss. Integrons PCR primer was designed along with sample pre-incubation and nested PCR method to enhance detection sensitivity for early detection of bacteria in fields. Designed integrons PCR primer successfully detected Xanthomonas arboricola pv. pruni from field collected samples, fruit, leaf, branch and even in raindrop collected from prune orchard. Pre-incubation along with nested PCR enhanced sensitivity to detect X. arboricola pv. pruni from seemingly healthy looking, symptomless branches. Designed integrons PCR can be used in prune nursery fields and in plant quarantine practice for the detection of X. arboricola pv. pruni.

• Journal of Korean Society on Water Environment
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• v.28 no.3
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• pp.453-459
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• 2012

In this study, Song-Cheon river Basin located at Dae-kwan-lyong Meyn in Kang-Won Province was monitored to analyze characteristics of non-point source pollution during the snowmelt period. The characteristics of NPS pollution such as runoff, change in water quality and pollutant loads by snowmelt were investigated from 23th February 2010 to 27th April 2010. The results showed that The climate change effects flow, turbidity and concentration of SS and COD with showing similar trend of fluctuation. Daily pollutant loads per unit area were SS 74.43 kg/ha/day, COD 1.25 kg/ha/day, BOD 0.21 kg/ha/day, TN 0.717 kg/ha/day, TP 0.141 kg/ha/day and the flow weight mean concentration has been SS 985.97 mg/L, COD 16.28 mg/L, BOD 2.67 mg/L, TN 9.302 mg/L, TP 1.834 mg/L, respectively. The flow and pollutant loads both of SS and of COD significantly increased during the snowmelt period. Because structurally loosen soil by freezing and thawing deteriorates water quality, research and management measures will be needed.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.4
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• pp.13-22
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• 2020

The Doam Lake watershed has a significant impact on the downstream water system due to nutrients and sediment outflow during rainfall caused by steep slopes, soil losses, and fertilization. These non-point sources are unclear in the discharge area and are affected by land use patterns, soil characteristics, and topographical features of the watershed. Therefore, this study conducted rainfall monitoring from July to October 2019 in Songcheon upstream of the Doam Lake watershed, one of the non-point pollution source management areas. Then, after analyzing rainfall runoff, Event Mean Concentration (EMC) and Mass First Flush ratio (MFFn) were calculated to compare and analyze the characteristics of rainfall and the non-point pollutant discharge. As a result of the analysis, it showed various non-point pollutant emission characteristics for each rainfall event. In addition, the concentration of EMC and the MFFn were affected by the average rainfall intensity and the maximum rainfall intensity, and were not significantly affected by the number of antecedent drying days. In the future, it is expected that effective non-point source reduction measures and management measures according to rainfall intensity through continuous monitoring and analysis will be needed.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.6
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• pp.43-50
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• 2020

The Doam Lake watershed is one of the non-point source management areas announced by the Ministry of Environment, and is a constant problem for the stream ecosystem dut to Storm water. In this study, a total of 48(rainfall) and 47(non-rainfall) sites were investigated for the entire watershed (Samyangcheon, Chahangcheon, Hoenggyecheon, Yongpyeongcheon, Songcheon, Lake Doam) on August 15, 2019 and on October 18, 2019 to estimate the source of turbid water in the Doam Lake watershed. Subsequently, water quality analysis was performed on Suspended Soild (SS), Turbidity, Total Phosphorus (TP), Total Nitrogen (TN), and Biochemical Oxygen Demands (BOD) and correlation among water quality parameters was analyzed based on the analyzed samples. As a result, most of the turbid water generated during rainfall was in highland fields. During rainfall, Hoengyecheon had the highest average SS concentration among all streams, and during non-rainfall, the average SS concentration was highest in Yongpyeongcheon, so the two stream were selected as vulnerable areas. However, since Yongpyeongcheon may be a temporary phenomenon due to river construction, additional continuous monitoring is required. Therefore, in the Doam Lake watershed, intensive management is required for vulnerable areas.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.3
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• pp.87-95
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• 2021

The Doam Lake Watershed is one of Gangwon-do's non-point source management areas. This area has a lot of snowfall in winter, and it is expected that there will be a lot of soil erosion in early spring due to snow melting. In this study, snow melting was monitored in the Doam Lake watershed from February to 3, 2020. It was conducted to analyze the water quality changes by calculating the concentration of non-point source pollution caused by snowmelt, and to compare the concentration of water quality during snowmelt event with rainfall and non-rainfall event. As a result of water quality analysis, Event Mean Concentration (EMC) at the first monitoring was SS 33.9 mg/L, TP 0.13 mg/L, TN 4.33 mg/L, BOD 1.35 mg/L, TOC 1.84 mg/L. At the second monitoring, EMC were SS 81.3 mg/L, TP 0.15 mg/L, TN 3.12 mg/L, BOD 1.32 mg/L, TOC 3.46 mg/L. In parameter except SS, it showed good water quality. It is necessary to establish management measures through continuous monitoring.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.455-458
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• 2001

To screen potato clones with high resistance to hollow heart (HH) and internal brown spot (IBS), field conditions were set up to induce high frequencies of physiological disorders in 'Atlantic' potato through various treatments of mulching, periodic shadings, and plant growth regulators such as trinexapac-ethyl (Tr-E) and dicloprop-triethanol mine (DTA). IBS occurrence was as high as 67.5% in the field plot mulched with transparent film and shaded for 10 days beginning at 80 days after planting. The highest level of HH was 22.9% in the plot mulched with transparent film and shaded for 10 days beginning at 60 days after planting. Very high level of IBS (66.3%) also occurred in the plot treated with 1500 mg/L of Tr-E 40 days after planting, while HH occurred by 21.3% in the plot treated with 1000 mg/L of DTA 70 days after planting. In the plots which were treated with 1,500 mg/L of Tr-E after 40 days of planting and 1000 mg/L of DTA after 70 days of planting, 'Superior' (moderately highly resistant) and 'Atlantic' (very susceptible) could be clearly distinguished to be resistant and susceptible. High occurrence condition set up in this study could be applied for the potato breeding program to screen potato clones with high resistance to HH and IBS.