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Expression of ${\alpha}_1$-Acid Glycoprotein and Inflammatory Cytokines during Differentiation of HL-60 Cells

  • Lee, Il-Ha;Kim, In-Sook;Lee, Soo-Young
    • BMB Reports
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    • v.33 no.5
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    • pp.402-406
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    • 2000
  • In order to understand the role of AGP on the differentiation of promyelocytic leukemia cells, the AGP expression and its relation to cytokines were investigated during granulocytic or monocytic differentiation of HL-60 cells. When HL-60 cells were treated with all-trans-retinoic acid (ATRA) for 5 days, the cells were fully differentiated into granulocytes, and the AGP mRNA and protein levels were continuously increased up to 5 days in a dose- and time- dependent manner. However, in the case of the monocytic differentiation of HL-60 cells by tetradeanoyl phorbol acetate (TPA), the AGP gene expression was not induced. In addition, $IL-1{\alpha}$, $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ mRNAs were also enhanced during granulocytic differentiation. These cytokine transcripts showed a peak level 3 days after the ATRA treatment. It decreased gradually thereafter. However, direct addition of recombinant cytokines ($IL-1{\beta}$, IL-6 and $TNF-{\alpha}$) and dexamethasone to the HL-60 cell cultures showed no AGP induction. These findings suggest that the AGP and proinflammatory cytokines are expressed in ATRA-treated promyelocytic cells. However, these cytokines do not act as autocrine inducers on AGP expression. This fact implies that the AGP expression during granulocytic differentiation of HL-60 cells is induced through a signal pathway different from hepatocyte signaling in inflammation.

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Crystallization of Poly(1,4-diaminobutane-co-adipic acid-co-ε-caprolactam-co-diethylenetriamine) Copolymer Fiber (Poly(1,4-diaminobutane-co-adipic acid-co-ε-caprolactam-co-diethylenetriamine) 공중합체 섬유의 결정거동)

  • Jo, Kuk Hyun;Song, Jihyeon;Cho, Hyun-Hok;Jang, Soon-Ho;Lee, Hyun Hwi;Kim, Nam Cheol;Kim, Hyo Jung
    • Textile Coloration and Finishing
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    • v.28 no.4
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    • pp.231-238
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    • 2016
  • We investigated the evolution of crystal formation as a function of drawing ratio in poly(1,4-diaminobutane-co-adipic acid-co-${\varepsilon}$-caprolactam-co-diethylenetriamine)(nylon 466T) copolymer formed by four monomers, i.e 1,4-diaminobutane, adipic acid, ${\varepsilon}$-caprolactam, diethylenetriamine(DETA), using synchrotron X-ray scattering measurement. In case of pristine(as spun) nylon 466T fiber, it was consisted with unoriented nylon $6{\alpha}$ and unoriented nylon $46{\alpha}$ phases. As increase the drawing ratio, unoriented nylon $6{\alpha}$ was transformed to oriented ${\gamma}$ phase, while unoriented nylon $46{\alpha}$ changed to oriented $46{\alpha}$ phase. The effect of the addition of DETA was not observed in the pristine fibers. However, DETA affected to restrict the formation of crystals at the maximum drawing condition, and as a result it had a role to increase the moisture regain.

An Effect of Carbon Arc Lamp and Low Level Laser Therapy on the Changes of Burned Mice's Cytokine (탄소방전등과 저강도 레이저 조사가 화상 쥐의 Cytokine 변화에 미치는 영향)

  • Park, Don-Mog;Park, Rae-Joon
    • The Journal of Korean Physical Therapy
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    • v.18 no.6
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    • pp.13-21
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    • 2006
  • Purpose: To provide the basic method for physical therapy to contribute to early stage treatment of a burned patients. We investigated whether or not carbon arc lamp treatment and low level laser one among various laser treatments have the effect of reducing Cytokine, and to elucidate the effect of carbon arc lamp treatment and laser one. Methods: The 6-week old BALB/c types of 92 mice were used for the experimental test, and they were burned with $100^{\circ}C$ water, they were divided into 4 groups in accordance with the method of treatment respectively. And the blood and the tissue from the subject of each group were extracted and analyzed each time after they were cured for 3, 6, 9 days. For the analysis of the results, SPSS statistical program was used in this study. Results: The quantity of $TNF-{\alpha}$ and $IL-1{\beta}$ within the blood of a burned mouse increased more than that of a normal mouse(p<0.01). In only a burned mouse, carbon arc lamp treatment gave the effect on the decrease of $TNF-{\alpha}$ thickness the 6th day to the 9th day(p<0.01). $IL-1{\beta}$ quantity was more decreased than that of control group around the 6th day. In comparison with only a control group, low level laser treatment has more significant effect in decreasing the quantity of $TNF-{\alpha}$ a and $IL-1{\beta}$ than the two different methods(p<0.01). In case that the two treatment methods, carbon arc lamp treatment and low level laser, were executed together, there was the effect of decreasing $TNF-{\alpha}$ until the 6th day(p<0.01). Conclusion: Both low level laser treatment and carbon arc lamp one would be able to have an effect on the inflammation inhibition of burned patients and tissues reproduction. However, it must be also considered for the two treatments to be done at the same time.

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Microstructure Change and Mechanical Properties in Binary Ti-Al Containing Ti3Al

  • Oh, Chang-Sup;Woo, Sang-Woo;Han, Chang-Suk
    • Korean Journal of Materials Research
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    • v.26 no.12
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    • pp.709-713
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    • 2016
  • Grain morphology, phase stability and mechanical properties in binary Ti-Al alloys containing 43-52 mo1% Al have been investigated. Isothermal forging was used to control the grain sizes of these alloys in the range of 5 to $350{\mu}m$. Grain morphology and volume fraction of ${\alpha}_2$ phase were observed by optical metallography and scanning electron microscopy. Compressive properties were evaluated at room temperature, 1070 K, and 1270 K in an argon atmosphere. Work hardening is significant at room temperature, but it hardly took place at 1070 K and 1270 K because of dynamical recrystallization. The grain morphologies were determined as functions of aluminum content and processing conditions. The transus curve of ${\alpha}$ and ${\alpha}+{\gamma}$ shifted more to the aluminum-rich side than was the case in McCullough's phase diagram. Flow stress at room temperature depends strongly on the volume fraction of the ${\alpha}_2$ phase and the grain size, whereas flow stress at 1070 K is insensitive to the alloy composition or the grain size, and flow stress at 1270 K depends mainly on the grain size. The ${\alpha}_2$ phase in the alloys does not increase the proof stress at high temperatures. These observations indicate that improvement of both the proof stress at high temperature and the room temperature ductility should be achieved to obtain slightly Ti-rich TiAl base alloys.

APPLICATION OF CONVOLUTION THEORY ON NON-LINEAR INTEGRAL OPERATORS

  • Devi, Satwanti;Swaminathan, A.
    • Korean Journal of Mathematics
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    • v.24 no.3
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    • pp.409-445
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    • 2016
  • The class $\mathcal{W}^{\delta}_{\beta}({\alpha},{\gamma})$ defined in the domain ${\mid}z{\mid}$ < 1 satisfying $Re\;e^{i{\phi}}\((1-{\alpha}+2{\gamma})(f/z)^{\delta}+\({\alpha}-3{\gamma}+{\gamma}\[1-1/{\delta})(zf^{\prime}/f)+1/{\delta}\(1+zf^{\prime\prime}/f^{\prime}\)\]\)(f/z)^{\delta}(zf^{\prime}/f)-{\beta}\)$ > 0, with the conditions ${\alpha}{\geq}0$, ${\beta}$ < 1, ${\gamma}{\geq}0$, ${\delta}$ > 0 and ${\phi}{\in}{\mathbb{R}}$ generalizes a particular case of the largest subclass of univalent functions, namely the class of $Bazilevi{\check{c}}$ functions. Moreover, for 0 < ${\delta}{\leq}{\frac{1}{(1-{\zeta})}}$, $0{\leq}{\zeta}$ < 1, the class $C_{\delta}({\zeta})$ be the subclass of normalized analytic functions such that $Re(1/{\delta}(1+zf^{\prime\prime}/f^{\prime})+1-1/{\delta})(zf^{\prime}/f))$ > ${\zeta}$, ${\mid}z{\mid}$<1. In the present work, the sucient conditions on ${\lambda}(t)$ are investigated, so that the non-linear integral transform $V^{\delta}_{\lambda}(f)(z)=\({\large{\int}_{0}^{1}}{\lambda}(t)(f(tz)/t)^{\delta}dt\)^{1/{\delta}}$, ${\mid}z{\mid}$ < 1, carries the fuctions from $\mathcal{W}^{\delta}_{\beta}({\alpha},{\gamma})$ into $C_{\delta}({\zeta})$. Several interesting applications are provided for special choices of ${\lambda}(t)$. These results are useful in the attempt to generalize the two most important extremal problems in this direction using duality techniques and provide scope for further research.

Protective Ability Index of Rust Layer Formed on Weathering Steel Bridge

  • Hara, S.;Kamimura, T.;Miyuki, H.;Yamashita, M.;Uchida, H.
    • Corrosion Science and Technology
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    • v.6 no.3
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    • pp.128-132
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    • 2007
  • For a quantitative inspection on the performance of weathering steel bridges, we have investigated the relationship between the corrosion rate and the composition of the rust layers formed on weathering steel bridges located in various environments in Japan and applied a protective ability index (PAI) to the bridges. The corrosion rates were clearly classified by the PAI, ${\alpha}/{\gamma}*$ and sub index of $({\beta}+s)/{\gamma}*$, where $\alpha$, \gamma*, $\beta$ and s are the mass ratio of crystalline $\alpha-FeOOH$, the total of $\gamma$-FeOOH+ $\beta$-FeOOH + the spinel-type iron oxide (mainly $Fe_3O_4$), $\beta-FeOOH$ and spinel-type iron oxide, analyzed by powder X-ray diffraction, respectively. In the case of ${\alpha}/{\gamma}$*>1, the rust layer works protective enough to reduce the corrosion rate less than 0.01 mm/y. The sub index $({\beta}+s)/{\gamma}*$<0.5 or >0.5 classifies the corrosion rate of the non-protective rust layers, therefore the former state of the rust layer terms inactive and the latter terms active. The quantitative inspection of a weathering steel bridge requires a performance-inspection (PI) and periodical deteriorationinspections (DI). The PI can be completed by checking of the PAI, ${\alpha}/{\gamma}*$. The DI on the weathering steel bridges where deicing salt is sprinkled can be performed by checking the PAI, $({\beta}+s)/{\gamma}*$.

Static and Dynamic Fracture Analysis for the Interface Crack of Isotropic-Orthotropic Bimaterial

  • Lee, Kwang-Ho;Arun Shukla;Venkitanarayanan Parameswaran;Vijaya Chalivendra;Hawong, Jae-Sug
    • Journal of Mechanical Science and Technology
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    • v.16 no.2
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    • pp.165-174
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    • 2002
  • In the present study, interfacial cracks between an isotropic and orthotropic material, subjected to static far field tensile loading are analyzed using the technique of photoelasticity. The fracture parameters are extracted from the full-field isochromatic data and the same are compared with that obtained using boundary collocation method. Dynamic photoelasticity combined with high-speed digital photography is employed for capturing the isochromatics in the case of propagating interfacial cracks. The normalized stress intensity factors for static cracks are greate. when ${\alpha}$: 90$^{\circ}$(fibers perpendicular to the interface) than when ${\alpha}$=0$^{\circ}$(fibers parallel to the interface), and those when ${\alpha}$=90$^{\circ}$are similar to ones of isotropic material. The dynamic stress intensity factors for interfacial propagating cracks are greater when ${\alpha}$=0$^{\circ}$ than ${\alpha}$=90$^{\circ}$. For the velocity ranges (0.1 < C/C$\sub$s1/<0.7) observed in this study, the complex dynamic stress intensity factor │K$\sub$D/│increases with crack speed c, however, the rate of increase of │K$\sub$D/│with crack speed is not as drastic as that reported for homogeneous materials.

Chemokines Expression in Children with a Non-productive Cough (소아기 단순 기침 환아에서의 케모카인 발현 양상 연구)

  • Lee, Young-Hwan;Kim, Hee-Sun
    • Journal of Yeungnam Medical Science
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    • v.24 no.2
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    • pp.129-136
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    • 2007
  • Purpose : To evaluate the chemokine expression in children with a non-productive cough. Materials and Methods : Six children with a non-productive cough who visited Yeungnam University Hospital were evaluated for the mRNA expression of interferon-${\gamma}$-inducible protein 10(IP-10), macrophage cationic protein 1 and 3 (MCP-1, 3), interleukin (IL)-8, regulated upon activation in normal T cells expressed and secreted (RANTES), eotaxin and growth-related oncogene-${\alpha}$ (Gro-${\alpha}$) using the reverse transcription polymerase chain reaction. Results : The chemokines IP-10 and MCP-3 were expressed in all samples. The chemokine RANTES was expressed in five cases, and IL-8 was expressed in three among them. However, eotaxin, Gro-${\alpha}$ and MCP-1 were not expressed at all. The expression of chemokine MCP-3, RANTES and IL-8 were suppressed after the resolution of coughing in just one available case. Conclusion : The chemokines MCP-3, RANTES and IL-8 may contribute to airway inflammation in children with a non-productive cough, whereas IP-10 is of secondary importance in this condition.

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Antioxidant Effect of Tryptophan and Arginine on Soybean oil Rancidity (Tryptophan과 Arginine의 대두유에 대한 항산화 및 상승효과)

  • Cho, Hee-Sook
    • Journal of the Korean Society of Food Culture
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    • v.14 no.1
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    • pp.21-28
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    • 1999
  • This study was conducted to investigate the antioxidant and synergistic effects upon different concentration(0.02, 0.1, 1%) of tryptophan and arginine of soybean oil during incubation at $60^{\circ}C$. Peroxide value(POV), acid value(AV) and thiobarbituric acid(TBA) value of each oil were monitored. Tryptophan and arginine showed antioxidative effects in all concentration. In case of incubating antioxidative effect of tryptophan was similar to that of TBHQ that of arginine was considerably higher than that of ${\alpha}-tocopherol$, but was lower than that of tryptophan. Synergistic effects among tryptophan, arginine and some food antioxidant were shown to be available in all substrates and the best effect was shown in substrate added compound of tryptophan and ${\alpha}-tocopherol$.

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Effect of V and Sb on the Characteristics of β to α Transformation in Zr-0.84Sn Alloy (V과 Sb 첨가가 Zr-0.84Sn 합금의 β→α 상변태 특성에 미치는 영향)

  • O, Yeong-Min;Jeong, Yong-Hwan-Jeong;Kim, Seon-Jin-Kim
    • Korean Journal of Materials Research
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    • v.12 no.4
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    • pp.317-323
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    • 2002
  • Effect of V and Sb content on characteristics of ${\beta}\;to\;{\alpha}$ phase transformation in Zr-0.84Sn alloy has been studied using optical microscopy and transmission electron microscopy. As V content increased, the ${\beta}{\to}{\beta}+{\alpha}$ transformation temperature was lowered, thus allowing the width of $\alpha$-lath in air-cooled Zr-0.86Sn-0.40V alloy to be fine. The width of ${\alpha}$-lath in air-cooled Zr-0.84Sn-xSb, however, was rarely changed with Sb content. The ${\beta}\;to\;{\alpha}$ transformed microstructures of water-quenched Zr-0.84Sn, Zr-0.84Sn-0.10V and Zr-0.84Sn-0.19V alloys were mainly slipped martensite. On the other hand, those of wafter-quenched Zr-0.86Sn-0.40V and Zr-0.85Sn-0.05Sb alloys were predominantly twinned martensite. In case of water-quenched Zr-0.85Sn-0.12Sb and Zr-0.84Sn-0.17Sb alloys, basketweave structure was observed. The transition of slipped martensite to twinned martensite in Zr-0.84Sn-xV alloys and the transition of twinned martensite to basketweave structure in Zr-0.84Sn-xSb alloys were due to the decrease of $M_s$ temperature.