• 한국정보처리학회논문지
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• 제6권12호
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• pp.3718-3726
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• 1999

칼라 영상은 단색조의 영상에 비해 인간의 시각을 크게 향상시킨다. 따라서, 칼라 영상 처리에 관한 연구는 매우 중요하다. 칼라 영상은 센서 잡음이나 채널 전송 에러에 의해 생기는 잡음에 의해 자주 오염되어진다. 이러한 칼라 잡음을 제거하기 위해 벡터 미디안, 평균 필터, 벡터 $\alpha-trimmed$ 평균 필터 등 여러 형태의 필터들이 개발되어왔는데 특히, 혼합된 칼라 잡음의 조건에서 벡터 $\alpha-trimmed$ 평균 필터는 우수한 성능을 보여왔다. 그러나, 벡터 $\alpha-trimmed$ 평균 필터는 필터링 과정이 영상의 전 영역에 걸쳐 동일한 가중치로 균일하게 적용되어지기 때문에 스텝 윤곽선 이동이 일어나고, 이에 따라 blurring 현상이 나타나는 문제점이 있다. 이러한 문제점을 개선하기 위해 본 논문에서는 윤곽선 영역과 평탄 영역을 구분한 뒤 각 영역에 적합한 선택적인 필터링을 하는 조건적인 퍼지 클러스터 필터를 제안하였고, 제안된 조건적인 퍼지 클러스터 필터는 혼합된 잡음의 조건에서 기존의 벡터 $\alpha-trimmed$ 평균 필터에 비해 NCD척도 및 사람의 시각에 의한 평가에 의해 우수한 성능을 보였다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2384-2389
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• 2007

The 2nd order response surface method (RSM) has been carried out to get optimum thermal design for enhanced heat transfer on square channel with bleed holes. The RSM was used as an optimization technique with Reynolds-averaged navier-stokes equation. Turbulence model for heat transfer analysis used RNG k-epsilon model. The wall function used enhanced wall function. Numerical local heat transfer coefficients were similar to the experimental tendency. Two design variables such as attack angle of rib (${\alpha}$), rib pitch-to-rib height ratio (p/e) were chosen. Operation condition considered bleeding ratio per bleed hole ($BR_{hole}$). A response surface were constructed by the design variables and operation condition. As a result, adjusted $R^2$ was more than 0.9. Optimization results of various objective function were similar to heat transfer in channel with and without bleed flow. But friction factor was lower than channel without bleed flow.

• BMB Reports
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• 제40권3호
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• pp.302-314
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• 2007

N type calcium channels (CaV2.2) play a key role in the gating of transmitter release at presynaptic nerve terminals. These channels are generally regarded as parts of a multimolecular complex that can modulate their open probability and ensure their location near the vesicle docking and fusion sites. However, the proteins that comprise this component remain poorly characterized. We have carried out the first open screen of presynaptic CaV2.2 complex members by an antibody-mediated capture of the channel from purified rat brain synaptosome lysate followed by mass spectroscopy. 589 unique peptides resulted in a high confidence match of 104 total proteins and 40 synaptosome proteome proteins. This screen identified several known CaV2.2 interacting proteins including syntaxin 1, VAMP, protein phosphatase 2A, $G_{o\alpha}$, G$\beta$ and spectrin and also a number of novel proteins, including clathrin, adaptin, dynamin, dynein, NSF and actin. The unexpected proteins were classified within a number of functional classes that include exocytosis, endocytosis, cytoplasmic matrix, modulators, chaperones, and cell-signaling molecules and this list was contrasted to previous reports that catalogue the synaptosome proteome. The failure to detect any postsynaptic density proteins suggests that the channel itself does not exhibit stable trans-synaptic attachments. Our results suggest that the channel is anchored to a cytoplasmic matrix related to the previously described particle web.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.44-44
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• 2003

$BK_{Ca}$ channels were suggested to contain one or more domains of the ‘regulator of K+ conductance’(RCK) in their cytosolic carboxyl termini (Jiang et al.2001). It was also shown that the RCK domain in mammalian $BK_{Ca}$ channels might sense the intracellular $Ca^{2+}$ with a low affinity (Xia et al. 2002). We aligned the amino acid sequence of the $\alpha$-subunit of rat $BK_{Ca}$ channels (rSlo) with known RCK domains and identified a second region exhibiting about 50％ homology. This putative domain, RCK2, contains the characteristic amino acids conserved in other RCK domains. We wondered whether this second domain is involved in the domain-domain interaction and the gating response to intracellular $Ca^{2+}$ for rSlo channel, as revealed in the structure of RCK domain of E. coli channel (Jiang et al.2001). In order to examine the possibility, site-directed mutations were introduced into the RCK2 domain of rSlo channel and the mutant channels were expressed in Xenopus oocytes for functional studies. One of such mutation, G772D, in the putative nucleotide-binding domain resulted in the enhanced $Ca^{2+}$ sensitivity and the channel gating of rSlo channel. These results suggest that this region of $BK_{Ca}$ channels is important for the channel gating and may form an independent domain in the cytosolic region of $BK_{Ca}$ channels. In order to obtain the mechanistic insights of these results, G772 residue was randomly mutagenized by site-directed mutagenesis and total 17 different mutant channels were constructed. We are currently investigating these mutant channels by electrophysiological techniques.ical techniques.

• Korean Journal of Acupuncture
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• 제29권3호
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• pp.467-475
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• 2012

Objectives : The aim of this study was to examine the effects of Acupuncture at the GV20 and GV22 on normal human beings using power spectrum analysis. Methods : Electroencephalogram(EEG) power spectrum exhibits site-specific and state-related differences in various frequency bands. 8 channels Background Electroencephalogram (EEG) was carried out in 30 subjects(24 females and 4 males). Results : In ${\delta}$(theta) band, the power values decreased significantly at the 8-channel average value(p=0.03) and especially at T3(p=0.02), T4(p=0.001) and P3(p=0.03). In ${\alpha}$(alpha) band, the power values have no significant changes. In ${\beta}$(beta)band, the power values increased significantly at the 8-channel average value (p=0.02) and especially at T4(p=0.003), P3 (p= 0.03) and P4(0.02). In ${\beta}/{\delta}$(beta/theta) ratio, the value increased significantly at the 8-channel average value(p=0.002) and especially at Fp2(p=0.05), F4(p=0.007), T3(0.012), T4(0.005), P3 (0.007) and P4(0.03) Conclusions : Through this data, we conclude that acupuncture at the GV20 and GV22 on normal human beings could have possibility to awake the cerebral cortex by the functional mechanism.

• The Korean Journal of Physiology and Pharmacology
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• 제13권3호
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• pp.241-249
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• 2009

Although extracellular $Ca^{2+}$ entry through the voltage-dependent $Ca^{2+}$ channels plays an important role in the spontaneous phasic contractions of the pregnant rat myometrium, the role of the T-type $Ca^{2+}$ channels has yet to be fully identified. The aim of this study was to investigate the role of the T-type $Ca^{2+}$ channel in the spontaneous phasic contractions of the rat myometrium. Spontaneous phasic contractions and $[Ca^{2+}]_i$ were measured simultaneously in the longitudinal strips of female Sprague-Dawley rats late in their pregnancy (on day 18 ${\sim}$ 20 of gestation: term=22 days). The expression of T-type $Ca^{2+}$ channel mRNAs or protein levels was measured. Cumulative addition of low concentrations (< 1 ${\mu}M$) of nifedipine, a L-type $Ca^{2+}$ channel blocker, produced a decrease in the amplitude of the spontaneous $Ca^{2+}$ transients and contractions with no significant change in frequency. The mRNAs and proteins encoding two subunits (${\alpha}$ 1G, ${\alpha}$ 1H) of the T-type $Ca^{2+}$ channels were expressed in longitudinal muscle layer of rat myometrium. Cumulative addition of mibefradil, NNC 55-0396 or nickel induced a concentration-dependent inhibition of the amplitude and frequency of the spontaneous $Ca^{2+}$ transients and contractions. Mibefradil, NNC 55-0396 or nickel also attenuated the slope of rising phase of spontaneous $Ca^{2+}$ transients consistent with the reduction of the frequency. It is concluded that T-type $Ca^{2+}$ channels are expressed in the pregnant rat myometrium and may play a key role for the regulation of the frequency of spontaneous phasic contractions.

• 정보통신설비학회논문지
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• 제2권2호
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• pp.36-45
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• 2003

본 논문은 무선 멀티미디어용 OFDM 시스템에 적용 할 수 있는 송신다이버시티 기술로 전송 효율 및 수신성능을 최대로 하기 위한 방안을 제시한다. 제안된 방법은 MIMO 채널에 적합한 시공간 부호기법으로 STTC(Space-Time Trellis Code)의 성능 분석을 위해 두 가지의 rms 지연확산, 즉 50 nsec, 150 nsec를 고려한 채널 추정에서 시평균을 위한 파라미터 $\alpha$가 0.3, 1인 경우와 하나의 프레임내에서 긴 훈련열 구간으로부터 추정된 채널 값을 해당 프레임에 적용한 경우의 성능을 비교 분석하였다. 실험결과 $\alpha$가 0.3인 경우가 긴 훈련 열만을 이용한 경우보다 BER이 $10^{-3}$에서 1.0dB의 SNR 개선을 가져옴을 알 수 있었으며, 또한 시평균을 위한 것이 그렇지 않은 경우보다 전반적인 성능 개선이 향상된다는 것을 확인하였다.

• Animal cells and systems
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• 제14권2호
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• pp.99-114
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• 2010

With the consensus sequence information of the serum glucocorticoid-induced protein kinase-1 (SGK1) phosphorylation site {R-X-R-X-X-(S/T)$\Phi$; where $\Phi$ is any hydrophobic amino acid}, we noticed that the transient receptor potential vanilloid 4 (TRPV4) cation channel, a member of the TRP vanilloid subfamily, harbors the putative SGK1 phosphorylation site (on its Ser 824). We have demonstrated that TRPV4 is an SGK1 authentic substrate protein, with the phosphorylation on the Ser 824 of TRPV4 by SGK1. Further, using TRPV4 mutants (S824A and S824D), we noted that the modification of the Ser 824 activates its $Ca^{2+}$ entry, and sensitizes the TRPV4 channel to 4-$\alpha$-phorbol 12,13-didecanoate (4-${\alpha}PDD$) or heat, simultaneously enhancing its active state. Additionally, we determined that the modification of the Ser 824 controls both its plasma membrane localization and its protein interactions with calmodulin. Thus, we have proposed herein that phosphorylation on the Ser 824 of TRPV4 is one of the control points for the regulation of its functions.

• 대한수의학회지
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• 제41권3호
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• pp.327-332
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• 2001

In recent years, experimental evidence have been suggested that melatonin has either contractive or relaxing effects on the vascular smooth muscle in vitro. But the effect of melatonin on the cardiovascular system in vivo had been emphasized about the hypotensive effect. In this work, we found not only hypotensive effect but also hypertensive effect of melatonin in rats and attempted to determine the mechanism of these effects elicited by melatonin. Regadless of concentration, melatonin(0.002~5 mg/kg) produced increase in mean blood pressure (MBP) in 36% (54/150 cases) and decrease in mean blood pressure in 64%(96/150 cases). As a whole melatonin caused an increase or a decrease in MBP without compensatory decrease or increase in heart rate. The melatonin-induced hypertension was abolished by the pretreatment of phenoxybenzamine, a ${\alpha}$-adrenoceptor antagoninst. The melatonin-induced hypotension was abolished by the pretreatment of propranolol, a ${\beta}$-adrenoceptor antagonist, ODQ, a NO-sensitive guanylate cyclase inhibitor, or nifedipine, a L-type $Ca^{2+}$ channel blocker, but not by bilateral cervical vagotomy. The results indicate that melatonin-induced hypertension may be related to ${\alpha}$-adrenoceptor stimulation and melatonin-indued hypotension may be related to ${\beta}$-adrenoceptor stimulation, inhibition of $Ca^{2+}$ channel and/or activation of guanylate cyclase.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 1992년도 수공학연구발표회논문집
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• pp.55-66
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• 1992

수로단면의 유속분포가 균일하지 않으므로, 에너지 원리의 $\alpha$값과 운동량원리의 $\beta$값 각각에 미치나, 대부분 실무에서는 이들 값을 추정하는 어려움 때문에 통상 1로 가정하여 사용하고 있다. 본 연구에서는 실용적으로 이용할 수 있는 공식을 유도하고, 기존에 사용되고있는 Prandtl-Von Karman 공식과 비교 검토하였으며, 유속분포계수 공식을 유도하기 위하여, 수로 바닥과 수 표면까지도 포함한 전 영역에서 비교적 정확한 유속분포를 나타내는 Chiu의 유속공식을 사용하였다. 또한, 실용적목적을 위해 유속데이타 없이 유속분포 계수를 구하기 위해서 Manning공식과 Chiu공식을 사용하였으며, 실험실 및 현장데이타를 위의 연구에 적용 검증하였다.