• Title/Summary/Keyword: alkalophilic bacteria alkaline amylase gene

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Molecular Cloning and Expression of Alkaline Amylase Gene of Alkalophic Bacillus sp. AL-8 and Enzyme Properties in E. coli (호알카리성 Bacillus sp. AL-8의 알카리성 아밀라제 유전자의 대장균에의 클로닝과 발현된 아밀라제의 특징)

  • Bae, Moo;Hwang, Jae-Won;Park, Sin-Hye
    • Microbiology and Biotechnology Letters
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    • v.15 no.6
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    • pp.441-445
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    • 1987

  • The gene coding for alkaline amylase of alkalophilic Bacillus sp. AL-8 was cloned and expressed in Escherichia coli which was lack of amylase activity. For the cloning of the alkaline amylase gene, the chromosomal DNA and plasmid vector pBR322 were cleaved at the site of EcoRI and the gene was cloned. The selection of the transformants carrying the amylase gene was based on the their antibiotics resistance and amylase activity of the transformants. The recombinant plasmids pJW8 and pJW200 containing 5.8Kb and 3.0Kb EcoRI inserts respectively were proved to can the alkaline amylase gene. Alkaline amylase expressed in E. coli was characterized. The enzyme was proved to be stable at the range of alkaline pH.

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