• 대한의생명과학회지
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• 제18권4호
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• pp.406-412
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• 2012

The polymorphism (insertion, I or deletion, D) of angiotensin converting enzyme (ACE) gene is designated as the presence of a 287 bp Alu repeat. The D/D homozygote carrier is associated with high ACE activity, and this high activity has been implicated with hypertension, coronary artery disease, or diabetic nephropathy. We studied the clinical candidate marker in ACE gene polymorphism using chemical and hematological analysis. The subjects are divided into normotensive and hypertensive groups and ACE genotype in the group was confirmed by PCR method. Chemical analysis was preceded with Hitachi7060, and hematological analysis was performed using Mythic 22. In 116 targeted people, 17 (38.64%) of 44 I/I genotype group are hypertension, 15 (34.09%) in 44 with D/I, but, D/D type in the 28 cases is 15 patients (53.57%) in hypertension. In hypertension group, biochemical analysis (triglyceride, and alkaline phosphatase) and hematological analysis (white blood cell, platelet) are showed high value in D/D genotype of ACE gene. The relationship between hypertension and ACE genotype is the same results as previously reported and we thought that the high laboratory value of white blood cell, platelet, triglycerides, and alkaline phosphatase are also indicator of hypertension in D/D type of ACE.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.53-59
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• 1990

A study was conducted to investigate the release pattern of zinc form the zinc containing boluses and to see whether the released zinc can cure a zinc deficiency in sheep. Three sheep were used in this experiment and were fed a low zinc semi-synthetic diet throughout the experimental period. Each sheep was given a single pre-weighed zinc containing bolus when blood variables showed continuous zinc deficiency. The zinc containing boluses when placed within the reticulo-rumen of zinc deficient sheep, release zinc at the rate of 106.6 mg zinc/day for 39 days. At the end of depletion period there was a reduced feed consumption, plasma zinc concentration, plasma alkaline phosphatase activity and increased plasma zinc binding capacity which were 409 g, 0.18 mg/l, 87 U/l and 88.7% respectively and 521 g, 0.18 mg/l, 142 U/l, and 89.5% respectively before first and second blousing. After the administration of the first and second boluses, the feed consumption, plasma zinc levels and plasma alkaline phosphatase activities rose rapidly and far exceeded the starting values. The zinc binding capacity was reduced to 21.9% due to the administration of the first and second boluses. It is concluded that zinc boluses can be used for curing a zinc deficiency in sheep.

• Journal of Trauma and Injury
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• 제26권3호
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• pp.81-88
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• 2013

Purpose: Among patients with multiple traumatic fractures, a tendency to form more callus exists in groups with multiple fractures combined with traumatic brain injury. This retrospective study evaluated the hematologic factors that might be useful to predict callus formation by comparing serologic tests and clinical and radiologic results in two groups. Methods: From January 2000 to December 2010, patients with femur shaft fractures were divided in two groups: one without traumatic brain injury (control group: 32 cases), and the other with traumatic brain injury (study group: 44 cases). We evaluated routine serologic exams and the amount of callus formation during the follow-up period. Results: Only the alkaline phosphatase level was statistically different between the two groups, not the White blood cell count, C-reactive protein, total calcium, and lactate dehydrogenase level. The amount of callus formation on the antero-posterior radiograph at the last follow up period was 74.9% in the study group and 42.1% in the control group. Then lateral radiograph showed 73.2% callus formation rate in the study group and 31.8% in the control group. Conclusion: In routine serologic exams, the two groups had no significant differences, except for the alkaline phosphatase level. The group with traumatic brain injury had much more callus formation, but there was no reliable factor to predict callus formation on the routine serologic exam.

• 대한방사선기술학회지:방사선기술과학
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• 제9권1호
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• pp.137-143
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• 1986

This study was undertaken to analyze the changes of radiographical image in avian (chicken) by using scanner and ${\gamma}-camera$ with $Tc^{99m}-MDP$. Experiment was aimed also to observe the changes of calcium, phosphorus and alkaline phosphatase activity in serum after experimental fracture. The results obtained are summerized as follows. 1. 10 chickens grown 6 weeks were scanned within 48 hours after experimental fracture, showed localized increase in uptake. The earlist increased local uptake was observed after 7 hours from experimental fracture at the fracture site. 2. Serum alkaline phosphatase level was increasing continuously during the fracture healing process in comparison with control group and serum alkaline phosphatase activity showed a peak after 2 weeks following the fracture. 3. The change of Ca. amount in serum trended to decrease from 1st day after fracture and it was in the lowest level at the 7 day's group following the fracture. 4. Serum phosphorus level trended to increase after fracture and it was in the highest level at the 7 day's group and showed similar level in comparison with control group.

• 한국식품영양과학회지
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• 제27권4호
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• pp.724-730
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• 1998

Effects of raw soy flour(RSY) and black(RSB) feeding on protein concentration of liver and serum, and GOT, GPT and alkaline phosphatase activities of serum in diabetic and nondiabetic rats were studied. Male rats(Sprague-Dawley), mean weight of (338.4$\pm$19.2g) were assigned to six dietary groups and fed with the assigned diet for 28 days. For each experimental, some rats were injected with streptozotocin intraperitoneally(L.P.) to induce diabets, and other rats were injected with buffer L.P. as a control group. The liver, kidney and spleen weights relative to bo요 weigth were higher in raw yellow soy flour diet diabetes(D-RSY) and black soy flour diet diabetes(D-RSB) groups than control, but the body weights were lower than control. The protein and albumin concentrations of liver and serum were lower in D-RSY and D-RSB groups than control. The albumin concentration of serum in D-RSB group was lower than control. The GOT activities of serum in RSY and RSB groups were increased compared with control, but the GPT activities were lower in diabetic control, D-RSY and D-RSB groups than control. The alkaline phosphatase activities of serum in RSY and RSB groups were higher than control, but those in D-RSY and D-RSB groups were lower than diabetic control.

• Journal of Microbiology
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• 제45권4호
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• pp.311-317
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• 2007

In this study, the effects of phosphate concentration and carbon source on the patterns of alkaline phosphatase (APase) and phospholipase (PLase) expression in Vibrio vulnificus ATCC 29307 were assessed under various conditions. The activities of these enzymes were repressed by excess phosphate (4 mM) in the culture medium, but this repression was reversed upon the onset of phosphate starvation in low phosphate defined medium (LPDM) containing 0.2 mM of phosphate at approximately the end of the exponential growth phase. The expressions of the two enzymes were also influenced by different carbon sources, including glucose, fructose, maltose, glycerol, and sodium acetate at different levels. The APase activity was derepressed most profoundly in LPDM containing fructose as a sole carbon source. However, the repression/derepression of the enzyme by phosphate was not observed in media containing glycerol or sodium acetate. In LPDM-glycerol or sodium acetate, the growth rate was quite low. The highest levels of PLase activity were detected in LPDM-sodium acetate, followed by LPDM-fructose. PLase was not fully repressed by high phosphate concentrations when sodium acetate was utilized as the sole carbon source. These results showed that multiple regulatory systems, including the phosphate regulon, may perform a function in the expression of both or either APase and PLC, in the broader context of the survival of V. vulnificus.

• 대한임상검사과학회지
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• 제43권1호
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• pp.6-11
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• 2011

In clinical chemistry tests, the interfering substances such as hemoglobin, lipid, bilirubin, and drugs, etc. can cause the changes of test results performed by spectrophotometrical methods. We evaluated the effects of interfering substances on the test results by adding interfering substances on the samples in the 19 kinds of clinical chemistry tests such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, gamma-glutamyltransferase, total protein, albumin, glucose, total cholesterol, total bilirubin, triglyceride, uric acid, calcium, inorganic phosphours, high density lipoprotein cholesterol, low density lipoprotein cholesterol, creatinine, blood urea nitrogen, and C-reactive protein using newly implemented automatic chemical analyzer Toshiba TBA-C8000 under the direction of CLSI EP07-A guideline. Hemolytic samples show increased concentration of total protein, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase and reduced concentration of total bilirubin, alkaline phosphatase by interfering effect. Hyperlipemic samples show increased concentration of total protein and alkaline phosphatase and reduced concentration of low density lipoprotein cholesterol. The samples with conjugated bilirubinemia show increased concentration of inorganic phosphours, otherwise the samples with unconjugated bilirubinemia show no interference or allowable range in the test result.

• 대한임상검사과학회지
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• 제46권3호
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• pp.79-84
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• 2014

Children and adolescents normally have higher Alkaline phosphatase levels than adults. This study was conducted to provide basic data about pediatric reference intervals for ALP. The data from the 2011 Korean National Health and Nutrition Examination Survey was used. Analysis was done for 847 Children and Adolescents (443 boys and 404 girls) aged 10 to 19. The method of p-NPP & EAE buffer was used to get the data. The highest ALP levels were found in boys aged 12 to 13, the reference interval being 653~1,518 IU/L; the levels decreased for those over 13 years old. In the case of girls, the highest ALP levels were found with those aged between 11 and 12, the reference interval being 463~1,598 IU/L; the levels decreased after 12 years of age. The mean difference in all age groups was statistically significant, except for those aged 10 to 11 and 11 to 12. Therefore, it is suggested that pediatric reference intervals should be divided into three groups according to gender. Complementing data about children and adolescents below 10 years old were not included in this study and will be much needed in the next trial.

• Biomolecules & Therapeutics
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• 제9권4호
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• pp.244-248
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• 2001

This study was performed to investigate therapeutic effects of Carthami Semen, Paeoniae Raidx extracts and chitosan on the growth and differentiation of human periodontal ligament cell. We found that co-treatment of methanol extracts of Carthami Semen and chitosan significantly increased the growth of human periodontal ligament cell. However, the sigle treatment groups of the extracts showed only 20-30% of the growth increase. Alkaline phosphase activity, one of differentiation markers, was increased approximately 1.5- fold by co-treatment of methanol extract of Carthami Semen and chitosan and calcified nodule formation was also increased at the similar levels as the alkaline phosphatase. But the single treatment groups showed only 20-30% increases. These results suggest that Carthami Semen and chitosan co-treatment can be used efficiently for periodontium regeneration.

• 한국동물학회지
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• 제31권2호
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• pp.147-155
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• 1988

1986년 12월부터 87년 11월까지 채집된 애응애과 응애를 분류학적으로 정리한 바 Brevipalpus속의 B.californicus와 B.ovovatus 2종과 다음의 한국4미기록종을 얻었다: Aegyptobia nothus Pritchard and Baker,Pentomerismus taxi Hailer, P. oregonensis McGregor,Brevipalpus lewisi McGregor. 따라서, 한국산 애응애과 응애는 Tenuipalpus속의 T.zhizhilashiliae(감나무애응애)를 포함하여 4속 7종이 보고되는 셈이다. 또한 P. oregonensis,B.californicus,B.ovovatus 및 B.lewisi에 대하여 polyacrylamide slab gel electrophoresis로 esterase와 alkaline phosphatase pattern을 조사한 바 esterase는 band수와 이동도에서 종과 속간에 뚜렷한 차이를 보였다.