• Title/Summary/Keyword: alkali soluble

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Nutritional Characteristics and Bioactive Components Contents of Flos Sophora Japonica (괴화(槐花)의 식품영양학적 접근 및 생리활성물질 함량분석)

  • Park, Sung-Jin;Chung, Byung-Hee;Choi, Young-Su;Kim, Jong-Dai;Park, Sung-Hye
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.1
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    • pp.171-180
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    • 2007
  • The purpose of this study is to determine the possibility of using Flos Sophora japonica as natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of Flos Sophora japonica a were measured. The contents of carbohydrate, crude protein, crude lipid and ash are 67.76%, 19.87%, 4.61% and 7.76%. And the calories of Flos Sophora japonica Linne was 318.32 Kcal. Total dietary fiber was 25.35% of total carbohydrates. The percentages of water soluble dietary fiber to insoluble dietary fiber were 1.80 % and 23.56 %, respectively. The protein were contained total 18 different kinds of amino acids. The contents of non-essential and essential amino acids were 4,898.78mg and 5,953.51mg. The K was the largest mineral followed by Ca, P and Mg, which means Flos Sophora japonica Linne is alkali material. The contents of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were 29.69%, 34.93% and 35.38%. Therefore, the amount of the total unsaturated fatty acid was higher than that of any other plant. The content of vitamin C in Flos Sophora japonica Linne was higher than that of any other plant, which suggest that it could increase blood elasticity. The content of rutin, which is responsible for capillary vessel permeability, was 22.60%. The contents of water soluble antioxidative materials in 1 mL of water-extracted Flos Sophora japonica Linne were 3.9 ${\mu}$g which is comparable to 1233.0 mmol of vitamin C in antioxidant effect. The general nutrients and other antioxidatant bioactive materials in Flos Sophora japonica Linne were also potential materials for good health food. It is expected that follow up study of Flos Sophora japonica Linne through developing processed food and evaluation of their functional properties would provide useful information as a source of medicinal foods.

Changes in Polysaccharides Content and Cell Morphology of Fomitopsis pinicola Mycelium during Submerged Culture (소나무잔나비버섯(Fomitopsis pinicola) 균사체 배양에 따른 함량 및 세포의 형태학적 변화)

  • Jung Yoo-Kyung;Shin Kyung-Ok;Park Hong-Duok;Kim Soon-Dong
    • Food Science and Preservation
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    • v.13 no.3
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    • pp.397-403
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    • 2006
  • This experiment was conducted to examine the changes in polysaccharide concentration and morphological variation of Fomitopsis pinicola mycelium during submerged-culture in the citrus peel medium (CP). On the 12 days culture, the yields of mycelium and alcohol insoluble substance were 40.21%(w/v) and 6.94%(w/w), respectively, which were much higher than 11.29%(w/v, wet basis) and 3.17%(w/w, wet basis) obtained from YM medium. A large amount of acid soluble polysaccharides was derived from YM medium while a larger amount of alkali soluble polysaccharide was produced from CP medium. Yields of the mycelium were higher when cultured in CP medium However, there was no significant difference in formation of membranous vesicle between mycelia cultured in CP medium and YM medium. It was also observed that the formation of vacuole was closely related to the activation of the multivescular body known as cytolysome. As a result activation of mycelium and cell wall biosynthesis were more accelerated in CP medium.

The Physicochemical Characteristics of Marinated Beef Galbi under Different Cooking Conditions (양념 소갈비의 조리과정에서의 물리화학적 특성 평가)

  • Hong, Sang-Pil;Kim, Young-Ho;Lee, Nam-Hyouck;Heo, Yeong-Uk
    • Journal of the Korean Society of Food Culture
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    • v.28 no.1
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    • pp.78-88
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    • 2013
  • Marinated beef galbi is a traditional Korean dish cooked with soy sauce, pear juice, onion, sesame oil, and sugar. However, there are many differences in beef galbi, including flavor and physicochemical aspects, depending on cooking conditions. Therefore, the physicochemical characteristics of marinated beef galbi prepared through various recipes was evaluated for its effects on pH, texture, aging, proteolysis, heating conditions, cooking time, and flavor compounds (pyrazines, IMPs, or FAAs). There were significant differences in salt concentration (0.8~3.03%), pH (4.89~6.22), and solid soluble contents (1.34-6.31 Brix) between recipes in this study. In the Pearson assay for sensory evaluation, overall preference correlated well with texture (a well-known sensory attribute in meat evaluation). Controlling the pH of meat through soaking in lemon solution, alkali water, phosphate, and baking powder solution, improved water holding capacity as much as 9 to 15% compared with the control. The myofibril index (MFI) of marinated meat stored at $4^{\circ}C$ increased 32% with 24 hours of aging and reached 39% at 48 hours of aging, and its fragmentation was observed through microscopy. SDS-PAGE showed hydrolysis of acid-soluble collagen by the pear juice, possibly related to meat tenderness. On the basis of surface temperature, the cooking time was estimated to be 8 minutes with pan heating at $170^{\circ}C$, 6 minutes at $270{\sim}300^{\circ}C$, and 4 minutes with charcoal at $700{\sim}900^{\circ}C$. Different pyrazine compounds, such as 2-methyl-3-phenylpyrrol(2,3-b) pyrazine (the typical product of the browning reaction) was mainly detected, and IMP (one of the main taste compounds in beef) was in higher amounts with the charcoal treatment, potentially related to its flavor preference among treatments. Our results demonstrate an effective case study and cooking system for beef galbi.

Study on the Damage Mechanism by Salt of White Porcelain Figurine in Underglaze Iron (백자 철화 인물형 명기의 염 손상 메커니즘 연구)

  • Lee, Sun Myung;Jin, Hong Ju;Yun, Ji Hyeon;Kwon, Oh Young
    • Journal of Conservation Science
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    • v.36 no.5
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    • pp.368-382
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    • 2020
  • It was confirmed that a white porcelain figurine in underglaze iron was damaged after exhibition. This study analyzes the current state of salt damage on the artifact and identifies the factors contributing to its deterioration by examining the material characteristics of the artifact and exhibition environment. The analysis will thus assist in preparing a conservation scheme for artifacts. The crystallized carbonate on the surface of the white porcelain figurine is a water-soluble alkali salt with high hygroscopicity and high solubility in water. This solubility increases as the temperature increases. The figurine was low-fired at approximately 1000℃. A lead glaze was applied, and thin cracks were formed on the glazed surface, indicating poor surface properties. Our analysis suggested that the showcase used in the exhibition likely created a moist environment resulting from condensation, as it was exposed to high temperature and relative humidity, particularly in comparison to the exhibition room where the temperature was regulated using an air conditioner. In addition, the artifacts in the showcase were exposed to sudden changes in temperature and relative humidity as the air conditioner was repeatedly turned on and off. Therefore, it can be deduced that the soluble salt remaining on the white porcelain figurine moved toward the surface of the relatively weak glaze as a result of the temperature, and the crystallized salt exacerbated surface damage as the moisture evaporated in a dry environment.

The Composition and Bioactivities of Ganoderan by Mycelial Fractionation of Ganoderma lucidum IY009 (영지 IY009 균사체의 분획에 따라 추출된 ganoderan의 조성과 생리적 활성)

  • Han, Man-Deuk;Jeong, Hoon;Lee, June-Woo;Back, Sung-Jin;Kim, Su-Ung;Yoon, Kyung-Ha
    • The Korean Journal of Mycology
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    • v.23 no.4 s.75
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    • pp.285-297
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    • 1995
  • Ganoderan, an immunomodulating ${\beta}-glucan$ of G. lucidum, induces potent antitumor immunity in tumor-bearing mice. The present study was set up to elucidate the chemical composition and bioactivities of ganoderan obtained from the mycelial fractionation of G. lucidum IY009. Ganoderan was isolated and purified from its extracellular, cell wall and cytoplasmic sources. These ganoderans were composed mainly of glucose. The cell wall-alkali soluble-water soluble fraction (CW-AS-WS) showed the highest antitumor activity (inhibition rate of 94%) in sarcoma-bearing mice and 37% of anticomplementary activity. The CW-AS-WS fraction was found to be approximately average 20,000 dalton in aq. 0.3N NaOH solution and composed of 88% carbohydrate and 4% protein. The carbohydrate of the CW-AS-WS was composed of 74% glucose. These results indicate that the ganoderans extracted from the mycelial fractionations of G. lucidum IY009 had different chemical characteristics and showed different potentiality in antitumor and anticomplementary activity.

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Two Cases of Inhalation Injury Caused by An Explosion of Two Different Chemical Disinfectants(Sodium Dichloroiso Cyanurate & Calcium Hypochlorite) in a Swimming Pool (수영장에서 소독제 분말 가스 폭발에 의한 흡입화상 2례)

  • Lee, Su Jin;Park, Eun Young;Kim, Mi Ran;Lee, Kon Hee;Kim, Kwang Nam
    • Clinical and Experimental Pediatrics
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    • v.46 no.2
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    • pp.198-202
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    • 2003
  • Of all toxic inhalant exposures, chlorine is one of the most common toxic chemical inhalants. When acutely inhaled, it can be responsible for symptoms ranging from upper air way irritation to more serious respiratory effects. It can also deteriorate lung function and lead to death. Chlorine and its compounds such as chlorinated cyanurates and hypochlorites are commonly used in water disinfection. The chemical agents discussed in this article are sodium dichloroiso cyanurate and calcium hypochlorite which are the two most popular products for swimming pool chlorination. They are both strong oxidizing agents which are soluble in water. Between the above two alkali agents, acid-base interaction occurred and generated heat. And the acid drove the combination of hypochlorous and chloride ions to form chlorine gas. We have experienced, two boys who had inhalation injuries caused by an accidental explosion which occurred in a swimming pool by mixing two different chlorinating agents : sodium dichloroiso cyanurate and calcium hypochlorite. The children suffered from respiratory difficulties after the exposure. They both required intensive care management and one needed the support of mechanical ventilation.

Micron-Sized Hollow Plastic Pigment (마이크론 크기의 중공 유기 안료)

  • Choi, Gwang-Sik;Kim, Yang-Soo;Jung, Hoon-Sang;Jang, Seo-Won;Kim, Nam-Seon
    • Polymer(Korea)
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    • v.33 no.5
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    • pp.463-468
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    • 2009
  • Syntheses of monodisperse and micron-sized hollow plastic pigment (HPP) were carried out through the core-shell reaction. The effects of the reaction parameters, such as the particle size, molecular weight, the swelling time, agitation rate, and the solid contect were investigated. This micron-sized HPP could be made by using the alkali soluble core with at least bigger than 200 nm size. To obtain a higher opacity ratio, the swelling time and molecular weight of the core should be controlled. The agitation rate affected the particle's morphology. To prevent the shell destruction, the agitation rate must be sufficiently low in case of the syntheses of micron-sized HPP. In this study, micron-sized HPP exhibiting the high hiding power and narrow particle distribution could be obtained.

Biomineralization Strategy of Biocomposites on Regenerated Shell: Chitin Synthesis and Regenerated Shell Formtation by Deformed Oyster Shell (생체복합체의 재생패각 합성전략: 참굴 패각의 변형에 따른 키틴 합성 및 패각재생)

  • Lee, Seungwoo;Park, Seungbin;Yeong, Donghee;Choi, Cheongsong
    • Korean Chemical Engineering Research
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    • v.46 no.3
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    • pp.529-534
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    • 2008
  • The normal shell and the regenerated oyster shell, Crassostrea gigas, are separated according to the characteristics of inner shell morphology. To study characteristics of chitin obtained from the regenerated shell, chitin prepared by acid and alkali process is analyzed by FT-IR (Fourier transform infrared spectrometer) and XRD (X-ray Diffractometer). The content of insoluble protein in the normal shell was more than doubled as compared with that in the regenerated shell. A comparison of secondary structure of the normal shell and the regenerated shell revealed that the content of random of the regenerated shell was above 47%, indicating an amount in the structural unordered state. Through amino acid composition analysis and secondary protein structure of soluble protein isolated from the normal shell and the regenerated shell, it was found that there are differences in biomineralization strategy of the regenerated shell as compared to the normal shell. The relatively low hardness of the regenerated shell is caused by the change of amino acid composition and ordered secondary protein structure as compared to hardness of the normal shell.

The Antimutagenic Activity of Sulfated Alginate and Its Effect on the Growth of Lactobacillus acidophillus (황 흡착 알긴산의 Lactobacillus acidophillus 성장에 미치는 영향 및 항 돌연변이 작용)

  • 강경금;박난희;김정목
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.757-761
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    • 2004
  • The alginate extracted from Hizikia fusiform by the acid alkali soluble alginate (AASA) extraction method was reacted with Na$_2$SO$_4$. The amount of sulfate absorbed in the 3% alginate solution was determined about 18,435 ppm by inductively coupled plasma-atomic emission spectrometry. Both alginate and S-alginate stimulated the growth of Lactobacillus acidophilus in MRS broth and peptone water medium. The addition of S-alginate to the media showed the higher bacterial numbers than alginate supplement. There was no mutagenic activity of S-alginate in the Ames test using the Salmonella Typhimurium TA98 and TA100 strains. The S-alginate showed suppressive effect against 2-NF and MMS of mutagens.

Preparation and Analysis of Yeast Cell Wall Mannoproteins, Immune Enhancing Materials, from Cell Wall Mutant Saccharomyces cerevisiae

  • Ha Chang-Hoon;Yun Cheol-Won;Paik Hyun-Dong;Kim Seung-Wook;Kang Chang-Won;Hwang Han-Joon;Chang Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.247-255
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    • 2006
  • Yeast cell wall matrix particles are composed entirely of mannoprotein and ${\beta}-glucan$. The mannoproteins of yeast cell wall can systemically enhance the immune system. We previously purified and analyzed alkali-soluble ${\beta}-glucans$ [${\beta}$-(1,3)- and ${\beta}$-(1,6)-glucans] [10]. In the present study, a wild-type strain was first mutagenized with ultraviolet light, and the cell wall mutants were then selected by treatment with 1.0 mg/ml laminarinase (endo-${\beta}$-(1,3)-D-glucanase). Mannoproteins of Saccharomyces cerevisiae were released by laminarinase, purified by concanavalin-A affinity and ion-exchange chromatography. The results indicated that the mutants yielded 3-fold more mannoprotein than the wild-type. The mannoprotein mass of mutant K48L3 was 2.25 mg/100 mg of yeast cell dry mass. Carbohydrate analysis revealed that they contained mannose, glucose, and N-acetylglucosamine. Saccharomyces cerevisiae cell wall components, mannoproteins, are known to interact with macrophages through receptors, thereby inducing release of tumor necrosis factor alpha ($TNF-{\alpha}$) and nitric oxide. Mannoprotein tractions in the present study had a higher macrophage activity of secretion of $TNF-{\alpha}$ and nitric oxide and direct phagocytosis than positive control ($1{\mu}g$ of lipopolysaccharide). In particular, F1 and F3 fractions in mannoproteins of K48L3 enhanced and upregulated the activity of nitric oxide secretion and macrophage phagocytosis by approximately two- and four-fold, respectively.