• Animal cells and systems
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• 제4권1호
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• pp.95-100
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• 2000

The regional distribution and relative frequencies of endocrine cells were studied immunogistochemically (PAP methods) in the alimentary tract of the tree frog, Hyla areorea japonica, using specific antisera against serotonin, somatostatin, bovine Sp-1/chromogranin (BCG), cholecystokinin (CCK)-8, vasoactive intestinal polypeptide (VIP), gastrin, bombesin, secretin and pancreatic polypeptide (PP). Six kinds of endocrine cells were identified in this study, These immunoreactive cells were located in the gastric glands of stomach regions and in the basal portion of the epithelium of the intestinal tract or esophagus with variable frequencies. They were spherical or spindle-shaped. Serotonin-immunoreactive cells were observed in the whole alimentary tract including the esophagus. Somatostatin-immunoreactive cells were also detected throughout the alimentary tract except the rectum. CCK-8-immunoreactive cells were observed from the pylorus to ileum. Vip-immunoreactive cells were restricted to the rectum. Bombesin-immunoreactive cells were restricted to the fundic gastric regions and gastrin-immunoreactive cells were restricted to the pylorus. However, no BCG-, secretin and PP-immunoreactive cells were demonstrated in this study. In conclusion, the regional distribution and relative frequency of the endocrine cells in the alimentary tract of the tree frog were similar to other anuran species, but some differences which may be caused by feeding habits and species specification were also observed.

• 대한수의학회지
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• 제40권1호
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• pp.17-26
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• 2000

The regional distribution and relative frequencies of endocrine cells were studied immunohistochemically (PAP methods) in the alimentary tract and pancreas of the toad, Bufo bufo gargarizans Cantor using specific antisera against bovine Sp-1/chromogranin (BCG), serotonin, bombesin, gastrin, substance P (SP), somatostatin, insulin, glucagon, pancreatic polypeptide (PP), vasoactive intestinal polypeptide (VIP) and secretin. Nine kinds of endocrine cells were identified in this study. Spherical or spindleshaped immunoreactive (IR) cells were located in the gastric glands of stomach regions, in the basal portion of the epithelium of intestinal tract or esophagus, and in the exocrine or pancreatic islets with variable frequencies. In the alimentary tract, BCG-IR cells were found in the fundus and pylorus with rare and a few frequencies, respectively. Serotonin-IR cells were demonstrated in the whole alimentary tract including the esophagus. Bombesin- and SP-IR cells were restricted to the stomach regions and gastrin-IR cells were restricted to the pylorus. Somatostatin-IR cells were detected throughout the whole alimentary tract except for the large intestine, However, insulin-, glucagon-, PP-, VIP- and secretin-IR cells were not detected in the alimentary tract. In the pancreas of toad, the distribution and relative frequency of endocrine cells were similar to those of other mammals. Insulin-IR cells were located in the central portion of the pancreatic islets and interspaces of exocrine portions, and glucagon-, somatostatin- and PP-IR cells were detected in the marginal regions of the pancreatic islets and interspaces of exocrine. However, other IR cells were not found in the pancreas. In conclusion, the regional distribution and relative frequency of the endocrine cells in the alimentary tract and pancreas of the toad were similar to other anuran species but some differences which might be caused by feeding habits and species specification were also observed.

• 대한수의학회지
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• 제39권4호
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• pp.689-697
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• 1999

The regional distribution and relative frequency of endocrine cells in the alimentary tract of the snake, Rhabdophis tigrinus tigrinus, were investigated by immunohistochemical method using 7 antisera. Chromogranin (Cg)-, glucagon-, somatostatin-, gastrin/cholecystokinin (Gas/CCK)-, serotonin-, bovine pancreatic polypeptide (BPP)-immunoreactive cells were identified in this study. Cg-immunoreactive cells were detected throughout the alimentary tract including the esophagus, with predominant frequency in the pylorus. Numerous immunoreactive cells were observed from the esophagus to the pylorus but a few cells were detected in the large intestine. Glucagon-immunoreactive cells were observed from the proximal portions to the distal portions of the small intestine. They were increased to the middle portions but thereafter decreased, and no cells were found in the terminal portions. Somatostatin-immunoreactive cells were restricted to the small intestine and these cells were decreased toward to distal portions of the small intestine. Gas/CCK-immunoreactive cells were detected in the pylorus and small intestine. They were most predominant in the pylorus and the proximal portions of the small intestine but thereafter decreased toward to the distal regions. Serotonin-immunoreactive cells were observed throughout the alimentary tract. They were most predominant in the pylorus and proximal portions of the small intestine but a few cells were observed in the large intestine. BPP-immunoreactive cells were restricted to the distal portions of the small intestine with rare frequency. No bombesin-immunoreactive cells were found in this study.

• 대한수의학회지
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• 제40권2호
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• pp.247-256
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• 2000

붉은귀거북이(red-eared slider, Trachemys scripta elegans)의 소화관에 존재하는 위장관내분비세포의 부위별 분포 및 출현빈도를 Grimelius 및 Masson-Hamperi(M-H)도은 염색을 이용하여 조직화학적으로 관찰하였다. 소화관은 식도, 기저부(fundus), 유문부, 십이지장, 공장, 회장 및 대장의 7부위로 구분하였다. 소화관 전 부위에 걸쳐 은호성세포(argy-rophil, Grimelius-positive cell) 및 은친화성세포(argentaffin cell, M-H-positive cell) 들이 관찰되었다. 타원형에서 방추형 또는 난원형에서 원형의 은호성세포 및 은친화성세포들이 기저부와 유문부의 위샘(gastric gland), 장 및 식도상피의 기저부에서 관찰되었으며, 소화관 각 부위별로 다양한 출현빈도를 나타내었다. 은호성세포들은 식도를 포함한 소화관 전 부위에서 관찰되었으며, 중등도의 출현빈도를 나타낸 직장에서 가장 높은 빈도를 나타내었고, 식도, 기저부, 십이지장, 공장 및 회장에서 극소수, 유문부에서는 소수 관찰되었다. 은친화성세포들 역시 소화관 전 부위에서 관찰되었으며, 은호성세포와 유사하게 다수의 출현빈도를 나타낸 직장에서 가장 높은 빈도를 나타내었고, 직장 이외의 부위에서는 소수의 세포들이 관찰되었다.

• 한국어류학회지
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• 제7권2호
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• pp.140-149
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• 1995

망상어, Ditrema temmincki 소화관의 형태 조직화학적인 특징은 다음과 같이 요약할 수 있다. 체장에 대한 식도에서 항문까지의 소화관 길이의 비 (RLG)는 0.89정도이며, 소화관에는 식도와 담관 입구사이의 팽창부인 위(胃)가 없다. 소화관은 형태 조직화학적인 특징에 의하여 식도, 식도 - 장이행부, 전장, 중장, 후장, 장 - 직장 이행부, 직장, 직장 - 항문 이행부, 항문으로 나눌 수 있다. 점막주름은 직장에서 가장 복잡한 형태를 보이며, 근육층은 식도와 항문에서 발달된 상태를 나타낸다. 점막주름의 상피층은 식도 전방부에서는 입방세포로 구성되며, 나머지 부위 에서는 원주상피로 구성된다. 소화관에는 PAS 반응에 양성을 나타내는 배상세포와 다당류 흡수세포 (Polysaccharid Absorptive Cell)가 관찰되는데, 후자는 다당류 계통의 영양물질의 흡수기능을 가진다. 망상어 소화관에서 다당류 계통의 영양물질은 주로 후장부에서 흡수된다.

• Journal of Chest Surgery
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• 제7권2호
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• pp.169-174
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• 1974

Duplication of the alimentary tract, especially of the esophagus, have been regarded as rare cong- enital anomalies. However, they are being reported with increasing frequency in the literature. In the . ,- past they have been described by a variety of names, such as "enteric cysts", "intestinal cysts", "giant diverticula", "`mediastinal cysts of foregut origin" "enterogenous cysts" and other descriptive terms. . Most authorities now agree that these anomalies are best described by the term "duplications of the alimentary tract. The duplications [of the alimentary tract] are spherical or tubular structures which poses a well developed smooth muscle layer and are lined with a mucous membrane from any part of the alimentary tract. They may occur at any place in the digestive tube from the tongue to the rectum and usually are intimately attached to some portion of the alimentary tube. We have experienced a duplication of the esophagus in 14 years old middle schoolboy. He complained dysphagia, eructation and substernal pain associated with intermittent high fever and chilliness, increasing in severity for recent three weeks. Routine chest X-ray film revealed nore markable abnormal finding but esophagogram. revealed marked narrowing of the esophagus throughout with a large blind pouch in lower half with fistulous communication at mid portion of the esophagus. On thoracotomy, a large infected blind pouch communicating with the lumen of normal esophagus proximally, Was extended from the level of 5th to 10th thoracic spine. The duplicated segment of the esophagus has a common muscular .wall and proximal communication with the adjacent esophagus. The infected, duplicated esophagus was segmentally resected, and esophagogastrostomy with pyloroplasty was done by displacing the stomach into the right thoracic cavity through midline laparotomy. His Postoperative course was uneventful and discharged without complication.

• 한국수산과학회지
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• 제29권4호
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• pp.438-449
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• 1996

태생 경골어류 망상어, Ditrema temmincki 체내자어의 소화관 발달을 영양분 흡수와 연관하여 조직화학적으로 조사하였다. 체내자어의 입은 부화직후 열리며, 소화관 말단부는 전장 4.0mm에 열린다. 소화관의 점막주름은 전장 5.0m 시기에 후방부에서 부터 형성된다. 전장 30.0mm에 체내자어의 소화관은 인두-식도-전장-중장-후장-직장-항문이 완전히 구별된다. 전장 50.0mm 이후에 소화관의 전체 내부조직상은 성체와 유사한 구조를 가진다. 체내자어가 전장 7.0mm일때 인두에서 처음으로 PAS에 양성반응을 보이는 점액세포가 출현한다. PAS에 양성반응을 보이는 배상세포는 장에서는 전장 25.0mm 이후, 직장에서는 전장 30.0 mm 이후, 항문에서는 전장 40.0mm 이후, 그리고 식도에서는 전장 50.0mm 이후에 나타난다. 체내자어의 난황물질은 전장 6.0mm 시기에 완전히 흡수된다. PAS에 양성반응을 보이는 다당류 흡수세포는 전장 7.0mm 일때 장후방부에서 처음 나타나며, 직장에서는 전장 13.0mm, 장전방부에서는 전장 15.0mm, 그리고 항문전방부에서는 전장 40.0mm부터 관찰할 수 있다. 임신기 동안 망상어 체내자어의 모체내 영양분 섭취 형태는 metrotrophy (모체의존형) 가운데 placental analogues type에 속한다.

• 대한한의학원전학회지
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• 제23권2호
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• pp.15-31
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• 2010

Objectives : The aim of this study was to understand application of Ganhap(干合) theory to human body. Methods : We investigated 5 important sphincters along the alimentary tract (Larynx, Pharynx, Cardia, Pyrolus, Iliocecal portion) comparing with the function of Viscera and Bowels, also with Meridian and collateral theory. Results & Conclusions : We can analyze 5 important sphincters along the alimentary tract into relationship of Ganhap. 1. Larynx are related with the combination of Byeong-Sin(丙辛合). 2. Pharynx are related with the combination of Jeong-Im(丁壬合). 3. The Cardia is related with the combination of Mu-Gyu(戊癸合). 4. The Pyrolus is related with the combination of Gap-Gi(甲己合). 5. Ileocecal portion is related with the combination of Eul-Gyeong(乙庚合).

• Journal of Life Science
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• 제10권2호
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• pp.17-20
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• 2000

Adenovirus which is an important infectious viral agent in respiratory and alimentary tract was investigated in Pusan, 1999. Fifteen cases of adenovirus were detected from stools and throat swabs of suspected patients. Two cases of enteric adenovirus were detected from a 5 years old boy and a 6-month-old boy. Thirteen cases of respiratory adenoviruses were detected from children aged under 10 years old and one adult. From respiratory specimens, 1 case of adenovirus type 2, 1 case of type 5, and 11 cases of type 3 were found. Enterotype 41 was detected from fecal preparations. Adenoviruses appeared mostly during winter months, January, February and December. Adenovirus showed a slowly progressive cytopathic effect on HEp-2 cells, Vero cells and BGM cells at 37$^{\circ}C$, in a 5-7% $CO_{2}$ incubation. An electron microscopic observation exhibited non-enveloped icosahedron with a diameter of 70nm. No significant differences on cytopathic effect and morphological features have been found from specimens of either alimentary tract or respiratory secretions.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제23권5호
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• pp.423-429
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• 2020

Purpose: Alimentary tract duplication (ATD) is a rare congenital condition that may occur throughout the intestinal tract. Clinical symptoms are generally related to the involved site, size of duplication, or associated ectopic mucosa. This study aimed to identify clinical implications by anatomical locations and age group and then suggest a relevant management according to its distinct features. Methods: We retrospectively reviewed the clinical data of pediatric patients who received a surgical management due to ATD. Furthermore, data including patients' demographics, anatomical distribution of the duplication, clinical features according to anatomical variants, and outcomes were compared. Results: A total of 25 patients were included in this study. ATD developed most commonly in the midgut, especially at the ileocecal region. The most common clinical presentation was abdominal pain, a sign resulting from intestinal obstruction, gastrointestinal bleeding, and intussusception. The non-communicating cystic type was the most common pathological feature in all age groups. Clinically, prenatal detection was relatively low; however, it usually manifested before the infantile period. A laparoscopic procedure was performed in most cases (18/25, 72.0%), significantly in the midgut lesion (p=0.012). Conclusion: ATD occurs most commonly at the ileocecal region, and a symptomatic one may usually be detected before the early childhood period. Surgical management should be considered whether symptom or not regarding its symptomatic progression, and a minimal invasive procedure is the preferred method, especially for the midgut lesion.