• 한국식품과학회지
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• 제28권2호
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• pp.384-388
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• 1996

다시마 추출액의 향미성분을 분석하기 위하여 steam distillation방법으로 추출한 뒤 GC/MS를 이용하여 분석하였다. 다시마 분말과 100에서 2시간 추출한 대조구 그리고 효소분해와 NaCl용액에서 추출한 뒤 ultrafiltration으로 점성 물질을 제거한 추출액을 비교하였다. 그 결과 다시마 분말의 주요 향미성분은 2-nonen-1-ol, myristic acid, palmitic acid와 oleic acid이었고 $100^{\circ}C$에서 열수추출한 대조구에서는 pentadecane, 1,4-cyclooctadiene과 myristic acid로 나타났으며, 점성물질을 제거한 시료에서는 cyclohexanone, (E,Z)-octa-1,5-dien-3-ol, acetic acid, ethanone, benzaldehyde, 2,6-nonadienal 등으로 나타났다. 또한, 점성물질제거 과정중 분자량이 비교적 큰 지방산들과 3,5-nonadien-2-ol, 1-penton-3-ol 등이 현저히 감소하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.305-306
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• 2003

To treat fulminant hepatic failure (FHF) patients, various extracorporeal bioartificial liver (BAL) systems have been developed. Several requirements should be met for the development of BAL systems: hepatocytes should be cultured in a sufficiently high density; their metabolic functions should be of a sufficiently high level and duration; and the BAL systems module should permit scaling-up and aseptic handling. Several investigators have found that freshly isolated primary hepatocytes can be cultured into three dimensional, tightly packed, freely suspended, multicellular aggregates, or spheroids. These specialized cell structures exhibited enhanced liver specific functions and a prolonged differentiated state compared to cells maintained in a monolayer culture. Cells in spheroids appear to mimic the morphology and ultrastructure of the in vivo liver lobule. The ability of hepatocytes to organize into three-dimensional structures was hypothesized to contribute to their enhanced liver-specific activities. In this study, the ammonia removal rate and urea secretion rate of pig hepatocytes spheroids encapsulated in Ca-alginate bead were determined. A packed-bed bioreactor with encapsulated pig hepatocytes was devised as BAL support system. The efficacy of the system was evaluated in vitro.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.756-762
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• 2001

As a component for a recirculating aquaculture system, a new strain of denitrifying bacterium was isolated from municipal sewage. The isolate was motile by means of one polar flagellum, catalase-positive, and a Gram-negative rod-shaped cell measuring $0.5-0.6{\mu}m$ in width and $1.3-1.9{\mu}m$ in length. The isolate was identified as Pseudomonas fluorescens and produced dinitrogen gas via the reduction of nitrate. The optimal growth conditions (pH, temperature, carbon source, and C/N ratio) of the isolate were found to be 6.8, $30^{\circ}C$, malate, and 3, respectively. Under optimal growth conditions of P. fluorescens, dinitrogen gas was first detected in the exponential growth phase, then a small amount of nitrite was developed and converted to dinitrogen gas in the stationary phase. Pseudomonas fluorescens cells were immobilized in modified polyvinyl alcohol (PVA) gel beads, and the maximum denitrification rate was measured as $36.6 {\mu}lN_2h^-1$ per bead with an optimum cell loading of $20mg {\mu}l^-1$ and $2\%$ sodium alginate added to the PVA gel. The operating stability of the modified PVA gel beads remained unchanged for up to 43 repeated batches.

• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.735-739
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• 2002

Large quantities of porcine hepatocyte aggregates with various degrees of aggregation (DA) could be obtained by controlling the suspension periods (0,9,24, and 48 h), and by entrapping the hepatocyte aggregates in model materials of encapsulation such as Ca-alginate and type-I collagen gels. The effects of DA on liver-specific functions of hepatocytes were evaluated in order to obtain optimum DA for the cell source of bioartificial liver (BAL) systems. Irregular rugged aggregates (size $75 \pm 28$ $\mu\textrm{m}$) farmed by 24 h of suspension culturing showed peak viability and hepatic functions such as ammonia removal and albumin secretion in the two types of entrapment systems, thus offering themselves as a stable cell source of a BAL system for hepatic functions and scale-up.

• 대한화장품학회지
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• 제49권1호
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• pp.67-74
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• 2023

본 연구에서는 천연 및 자연주의 화장품 시장에 대응하여 폴리비닐알코올(polyvinyl alcohol, PVA)을 이용한 필 오프 팩을 대체할 수 있는 성분을 탐색하고자 하였다. 수용성 다당류인 풀루란(pullulan)과 기타 다당류(sodium hyaluronate, cellulose gum, hydroxyethyl cellulose, sodium alginate, corn starch)와의 조합을 통한 필 오프 팩을 제조하였으며, 각 필 오프 팩의 pH와 점도, 온도별 안정도를 확인하였다. 제조한 필름의 두께와 인장 강도를 측정하여 기존의 PVA 필 오프 팩과 비교하였으며, 피부에서의 도포성과 건조속도, 제거성을 확인하였다. 그 중 pullulan-sodium hyaluronate 필오프 팩은 5.12% 얇은 두께와 4.23% 높은 필름 인장강도로 PVA 필 오프 팩을 대체할 수 있는 우수한 필름성을 보였다. 실제 피부에 적용 시 팩의 퍼짐 정도와 균일하게 도포 가능한 사용성, 건조 후 제거 시 필름의 형성 및 제거 강도 평가 또한 PVA 필 오프 팩과 흡사한 수준을 보였다. 이에 pullulan-sodium hyaluronate 필름은 천연 필 오프 팩으로 PVA 필 오프 팩을 대체할 수 있을 정도의 물성을 보임을 확인하였다.