• 한국해양바이오학회지
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• 제7권1호
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• pp.28-34
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• 2015

We examined cell regeneration efficiency of brown marine algae living in Jeju coast for search of a novel therapeutic device with cutaneous wound healing materials. The five algae were collected and compared with epidermal growth factor (EGF) as a positive control in the assays of cell proliferation and cell migration of NIH3T3 fibroblast cells. Among the 80% methanol extracts of these brown algae, the two algal extracts from Ishige foliacea and Colpomenia bullosa showed the proliferative effects of the cells similar to the effect of EGF. Besides it was found that Colpomenia bullosa extract significantly enhanced cell migration of NIH3T3 cell. In the study, therefore, we confirmed that the Colpomenia bullosa extract improved proliferation of NIH3T3 cell and a potential candidate for cultaneous wound healing.

• 생태와환경
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• 제36권2호통권103호
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• pp.161-171
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• 2003

본 연구는 도심에 위치한 얕은 인공호수의 수질변화 특성, 제한영양염 및 퇴적물 특성을 평가하기 위해서 이루어졌다. 일감호는 연 평균 엽록소 a($77.2{\pm}36.6\;{\mu}g/l)와 TP ($66.6{\pm}20.5\;{\mu}g/l)농도 그리고 영양상태지수 (>60)모두를 토대로 할 때 매우 부영양상태였다. 결빙시기와 해빙되는 시기에 수층내 무기질소와 인의 수체내 농도가 증가하였다가 여름철에는 감소하는 계절적인 변화가 나타났다. 수체내 DIN/DIP(무게비)비율과 TSI편차분석 그리고 생물검정 (AGP)실험을 통해서 질소와 인 모두가 식물플랑크톤의 성장을 제한함이 제시되었다. TSI편차분석 결과에서는 1년 중 생물검정을 통해서도 인 제한이 예측된 바 있는 9월부터 11월에 인과 질소 모두의 식물플랑크톤 성장에 대한 제한정도가 가장 큰 것으로 나타났다. 퇴적물내 인 함량(313${\pm}$155mgP/kg)은 계절에 따른 변화를 나타났고, 인용출률은 pH9에서 0.29${\pm}$0.02Pmg $m^{-2}$ $day^{-1}$로 가장 높았다. 질소함량은 평균 4,452${\pm}$283.0 mg N/kg였으며 계절에 따른 큰 차이는 없었다. 이러한 결과들은 퇴적물이 제한영양염의 공급원으로서의 중요한 역할을 수행하며, 퇴적물내 인 함량의 계절에 따른 변화가 조류의 생물량을 조절할 수 있음을 의미한다.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1547-1554
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• 2015

The potential of microalgae biofuel has not been realized because of the low productivity and high costs associated with the current cultivation systems. In this study, a new low-cost and transparent attachment material was tested for cultivation of a filamentous algal strain, Stigeoclonium sp., isolated from wastewater. Initially, the different materials tested for Stigeoclonium cultivation in untreated wastewater were nylon mesh, polyethylene mesh, polypropylene bundle (PB), polycarbonate plate, and viscose rayon. Among the materials tested, PB led to a firm attachment, high biomass (53.22 g/m², dry cell weight), and total lipid yield (5.8 g/m²) with no perceivable change in FAME profile. The Stigeoclonium-dominated biofilm consisted of bacteria and extracellular polysaccharide, which helped in biofilm formation and for effective wastewater treatment (viz., removal efficiency of total nitrogen and total phosphorus corresponded to ~38% and ~90%, respectively). PB also demonstrated high yields under multilayered cultivation in a single reactor treating wastewater. Hence, this system has several advantages over traditional suspended and attached systems, with possibility of increasing areal productivity three times using Stigeoclonium sp. Therefore, multilayered attached growth algal cultivation systems seem to be the future cultivation model for large-scale biodiesel production and wastewater treatment.

• Ocean and Polar Research
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• 제25권4호
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• pp.625-631
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• 2003

While phytoplankton diversity and productivity in the Southern Ocean has been widely studied in recent years, most attention has been given to elucidating environmental factors that affect the dynamics of micro-plankton (mainly diatoms) and nano-plankton (mainly Phaeocystis antarctica). Only limited effects have been given to studying the occurrence and the potential risks associated with the blooming of dinoflagellates in the relevant waters. This study focused on the appearance and toxicological characteristics of a toxic dinoflagellate, Alexandrium tamarense, identified and isolated from the Drake Passage in a research cruise from November to December 2001 The appearance of A. tamarense in the Southern Ocean indicates the risk of a paralytic shellfish poisoning (PSP) outbreak there and is therefore of scientific concern. Results showed that while the overall quantity of A. tamarense in water samples from 30meters below the sea surface often comprised less than 0.1% of the total population of phytoplankton, the highest concentration of A. tamarense (20 cells $L^{-1}$) was recorded in the portion of the Southern Ocean between the southern end of South America and the Falkland Islands. Waters near the Polar Front contained the second highest concentrations of 10-15 cells $L^{-1}$. A. tamarense was however rarely found in waters near the southern side of the Polar Front, indicating that cold sea temperatures near the Antarctic ice does not favor the growth of this dinoflagellate. One strain of A. tamarense from this cruise was isolated and cultured for further study in the laboratory. Experiments showed that this strain of A. tamarense has a high tolerance to temperature variations and could survive at temperatures ranging from $5-26^{\circ}C$. This shows the cosmopolitan nature off. tamarense. With regard to the algal toxins produced, this strain of A. tamarense produced mainly C-2 toxins but very little saxitoxin and gonyailtoxin. The toxicological property of this A. tamarense strain coincided with a massive death of penguins in the Falkland Islands in December 2002 to January 2003.

• 한국환경과학회지
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• 제21권9호
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• pp.1079-1085
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• 2012

Ground seawater quality was investigated, and the algal growth potential (AGP) tests were performed along the Tongyeong coast to examine the inflow of materials, which is needed for the red tide species Cochlodinium polykrikoides to grow. The study was conducted to determine the mechanism for C. polykrikoidesred tides. Water temperature, salinity, pH, and dissolved oxygen (DO) ranged from 16.05 to $20.74^{\circ}C$, 18.20 to 32.11 psu, 6.00 to 7.61, and 3.41 to 7.91 mg/L (41.4-96.1%), respectively. No seasonal variation was observed in water temperature. The salinity, pH, and DO saturations at most stations were lower than those of coastal seawater at Tongyeong. The ${NH_4}^+$-N, ${NO_{2+3}}^-$-N, ${PO_4}^{3-}$-P concentrations ranged from 0.43 to 16.00 ${\mu}M$, 1.50 to 132.38 ${\mu}M$, and 1.30 to 6.29 ${\mu}M$, respectively; the values at some stations were much higher than observed in Tongyeong coast seawater. Using seawater from station B, C. polykrikoides grew consistently, with a high growth rate, similar to the red tide in nature. This seawater appeared to contain materials needed by C. polykrikoides to grow. Therefore, C. polykrikoides red tides seem to occur wherever the ground sea water contains materials that are needed for its growth.

• Environmental Engineering Research
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• 제21권2호
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• pp.211-218
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• 2016

Cheonggye-cheon is a partly restored urban stream located in central Seoul. We monitored fecal contamination using three different fecal indicators, total coliforms (TC), fecal coliforms (FC) and E. coli, to assess differences in each indicator on days of varying weather conditions. Presumptive TC, FC and E. coli colonies were identified by their 16S rRNA sequences. The results showed that enumeration of E. coli provided a better reflection of fecal contamination of the stream than TC and FC. The main sources of contamination were the inflow of fecal-polluted groundwater from the vicinity of a subway line and two inflowing streams. The fecal contamination was worsened on days with heavy rain because untreated sewage from a collecting facility flowed into the stream. Moreover, growth potential of fecal indicator (E. coli) in situ induced by algal exudates was measured. Our results suggest that an appropriate standard based on E. coli rather than TC and FC should be established for improving water quality management strategies of Cheonggye-cheon in the future.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.410-418
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• 2023

Bacilysin is a dipeptide antibiotic composed of L-alanine and L-anticapsin produced by certain strains of Bacillus subtilis. Bacilysin is gaining increasing attention in industrial agriculture and pharmaceutical industries due to its potent antagonistic effects on various bacterial, fungal, and algal pathogens. However, its use in industrial applications is hindered by its low production in the native producer. The biosynthesis of bacilysin is mainly based on the bacABCDEF operon. Examination of the sequence surrounding the upstream of the bac operon did not reveal a clear, strong ribosome binding site (RBS). Therefore, in this study, we aimed to investigate the impact of RBS as a potential route to improve bacilysin production. For this, the 5' untranslated region (5'UTR) of the bac operon was edited using the CRISPR/Cas9 approach by introducing a strong ribosome binding sequence carrying the canonical Shine-Dalgarno sequence (TAAGGAGG) with an 8 nt spacing from the AUG start codon. Strong RBS substitution resulted in a 2.87-fold increase in bacilysin production without affecting growth. Strong RBS substitution also improved the mRNA stability of the bac operon. All these data revealed that extensive RBS engineering is a promising key option for enhancing bacilysin production in its native producers.

• 환경생물
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• 제31권4호
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• pp.288-294
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• 2013

한국산 고지질 Botryococcus를 확보하기 위해 시료채취를 하였고, 실험실에서 Botryococcus sp.를 분리하였다. 분리된 Botryococcus sp.는 현미경으로 그 크기와 형태를 관찰하였고, 분자적 동정을 위해 18S rRNA gene과 ITS region의 염기서열을 분석함으로써 Botryococcus sudeticus J2로 명명하였으며, 생태학적 특성 연구를 위해 문헌조사를 통해 국내의 Botryococcus 속의 분포를 조사를 실시하였다. 동정된 B. sudeticus J2의 생리적 특성 분석을 위해 배양실험을 수행하였고, 생장률과 바이오매스 생산성 그리고 광합성효율을 측정하였다. 또한 바이오디젤 생산을 위한 조류주로서의 가치를 판단하기 위해 총 지질함유량과 지방산 조성을 분석하였다. B. sudeticus J2의 생태적, 생리적 연구 후 2배의 광량과 2% $CO_2$ 조건에서 배양을 수행함으로써 최적 배양조건을 탐색하고자 하였다. 분리된 B. sudeticus J2는 비교를 위한 B. braunii UTEX 572보다 높은 바이오매스 생산성과 지질생산성을 보였으나 바이오매스 생산을 위한 다른 후보 미세조류에 비해서는 낮은 생장률을 보였다. 따라서 바이오디젤 생산을 위한 조류로 B. sudeticus J2를 활용하기 위해서는 본 연구에서 밝혀진 광저해에 대한 내성에 초점을 맞추어 최적 배양을 위한 광조건을 탐색하는 연구가 필요하다.

• ALGAE
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• 제32권4호
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• pp.309-324
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• 2017

Recently, the heterotrophic nanoflagellate Katablepharis japonica has been reported to feed on diverse red-tide species and contribute to the decline of red tides. However, if there are effective predators feeding on K. japonica, its effect on red tide dynamics may be reduced. To investigate potential effective protist predators of K. japonica, feeding by the engulfment-feeding heterotrophic dinoflagellates (HTDs) Oxyrrhis marina, Gyrodinium dominans, Gyrodinium moestrupii, Polykrikos kofoidii, and Noctiluca scintillans, the peduncle-feeding HTDs Luciella masanensis and Pfiesteria piscicida, the pallium-feeding HTD Oblea rotunda, and the naked ciliates Strombidium sp. (approximately $20{\mu}m$ in cell length), Pelagostrobilidium sp., and Miamiensis sp. on K. japonica was explored. We found that none of these heterotrophic protists fed on actively swimming cells of K. japonica. However, O. marina, G. dominans, L. masanensis, and P. piscicida were able to feed on heat-killed K. japonica. Thus, actively swimming behavior of K. japonica may affect feeding by these heterotrophic protists on K. japonica. To the contrary, K. japonica was able to feed on O. marina, P. kofoidii, O. rotunda, Miamiensis sp., Pelagostrobilidium sp., and Strombidium sp. However, the specific growth rates of O. marina did not differ significantly among nine different K. japonica concentrations. Thus, K. japonica may not affect growth of O. marina. Our findings suggest that the effect of predation by heterotrophic protists on K. japonica might be negligible, and thus, the effect of grazing by K. japonica on populations of red-tide species may not be reduced by mortality due to predation by protists.

• 한국해양학회지:바다
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• 제16권1호
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• pp.27-38
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• 2011

동계 진해만 광역해역의 식물플랑크톤의 군집구조와 영양염 제한 특성을 파악하고자 식물플랑크톤 군집의 정점간 비교와 환경인자간의 관계를 조사하였다. 아울러 식물플랑크톤의 성장에 영향을 미치는 제한영양염을 평가하기 위해서 현장수를 이용하여 생물검정실험을 수행하였다. 조사기간동안 규조류와 은편모조류는 대부분의 정점에서 전체 식물플랑크톤의 90% 이상을 점유하였고, 식물플랑크톤의 현존량은 마산만이 진해만 보다 현저히 높게 관찰되었다. DIN:DIP 비, pH, 투명도는 식물플랑크톤의 생물량과 유의한 양(+)의 관계를 보였다. 식물플랑크톤 군집구조를 바탕으로 Cluster 분석과 MSD 분석을 한 결과 크게 3개의 그룹으로 구분되었다. 제 1그룹은 고성과 통영을 중심으로 한 진해만 서부해역으로 은편모조류가 전체 식물플랑크톤 현존량에 50% 이상으로 우점하였다. 제 2그룹은 마산만 가장 내측해역으로 높은 생물량, 낮은 투명도, 높은 DIN:DIP 비가 관찰된 것이 특이적이였다. 제 3그룹은 진해만 동부수역에 해당되는 마산만 외측과 진해만 중앙해역으로 구분이 되었고, 이들 정점의 특성은 DSi:DIP 비가 높았고, 규조류가 우점출현하였다. 영양염 절대농도와 구성성분비에 의한 제한영양염과 생물검정실험을 통한 영양염 제한은 마산만 대부분의 정점은 P 영양염의 제한이 관찰되었고, 진해만은 N기원의 영양염제한이 식물플랑크톤의 증식에 중요한 역할을 하는 것으로 파악되었다. 동계 진해만은 규산염이 상대적으로 풍부하게 존재하였고, 이는 규조류가 우점하기 좋은 환경이라 사료된다.