• ALGAE
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• 제25권2호
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• pp.99-104
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• 2010

A psychrotolerant cyanobacterium, Nodularia spumigena KNUA005, was isolated from a cyanobacterial bloom sample collected near Dasan Station in Ny-${\AA}lesund$, Svalbard Islands during the Arctic summer season. To generate an axenic culture, the isolate was subjected to three purification steps: centrifugation, antibiotic treatment and streaking. The broad antibacterial spectrum of imipenem killed a wide range of heterotrophic bacteria, while the cyanobacterium was capable of enduring both antibiotics, the remaining contaminants that survived after treatment with imipenem were eliminated by the application of an aminoglycoside antibiotic, kanamycin. Physical separation by centrifugation and streaking techniques also aided axenic culture production. According to the cold-tolerance test, this mat-forming cyanobacterium was able to proliferate at low temperatures ranging between 15 and $20^{\circ}C$ which indicates the presence of cold-tolerance related genes in N. spumigena KNUA005. This suggests the possibility of incorporating cold-resistance genes into indigenous cyanobacterial strains for the consistent production of algae-based biofuel during the low-temperature seasons. Therefore, it is needed to determine the cold-tolerance mechanisms in the Arctic cyanobacterium in the next research stage.

• 청정기술
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• 제23권4호
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• pp.435-440
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• 2017

바이오 연료 생산을 위한 3세대 바이오매스, 즉 미세조류 및 거대조류의 급속 열분해는 최근 1 세대 및 2 세대 바이오매스와 비교하여 실험적으로 연구된 바 있다. 하지만 거대조류의 경우 스케일업을 위한 공정모사 및 공정설계 연구는 거의 전무한 실정이다. 이 연구에서는 갈조류 급속 열분해의 벤치 스케일 실험 데이터에 근거하여 갈조류로부터 최종적으로 디젤을 생산하는 산업 규모의 공정을 상용 공정모사기를 이용하여 설계하고 모사하였다. 이때 육상 바이오매스 대비 갈조류의 조성 차이를 수용하기 위해 공정 설계에 특별한 주의를 기울였다. 연간 380,000톤의 건조 갈조류 원료를 바이오 디젤로 전환하는 전체 공정을 경제적으로 평가하고 최소 (디젤) 판매 가격 또한 산정하였다.

• ALGAE
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• 제28권1호
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• pp.93-99
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• 2013

A freshwater cyanobacterium, Phormidium autumnale KNUA026, was isolated from puddles of icy water in Gyeongsan City, South Korea and its potential as a biofuel feedstock was investigated. Maximal growth was obtained when the culture was incubated at $25^{\circ}C$ and around pH 9.0. The total lipid content of the isolate was approximately 14.0% of dry weight and it was found that strain KNUA026 was able to autotrophically synthesize heptadecane ($C_{17}H_{36}$) which can be directly used as fuel without requiring a transesterification step. As this benthic cyanobacterium was capable of forming thick mats, it could be easily harvested by gravitational settling and this property may reduce the cost of production in commercial applications. Hence, P. autumnale KNUA026 appears to be a promising resource for use in the production of microalgae-based biofuels.

• International journal of advanced smart convergence
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• 제4권1호
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• pp.162-178
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• 2015

Petroleum based fossil fuels used to power most processes today are non-renewable fuels. This means that once used, they cannot be reproduced for a very long time. The maximum combustion of fossil fuels occurs in automobiles i.e. the vehicles we drive every day. Thus, there is a requirement to shift from these non-renenewable sources of energy to sources that are renewable and environment friendly. This is causing the need to shift towards more environmentally-sustainable transport fuels, preferably derived from biomass, such as biodiesel blends. These blends can be made from oils that are available in abundance or as waste e.g. waste cooking oil, animal fat, oil from seeds, oil from algae etc. Waste Cooking Oil(WCO) is a waste product and so, converting it into a transportation fuel is considered highly environmentally sustainable. Keeping this in mind, a life cycle assessment (LCA) was performed to evaluate the environmental implications of replacing diesel fuel with WCO biodiesel blends in a regular Diesel engine. This study uses Life Cycle Assessment (LCA) to determine the environmental outcomes of biodiesel from WCO in terms of global warming potential, life cycle energy efficiency (LCEE) and fossil energy ratio (FER) using the life cycle inventory and the openLCA software, version 1.3.4: 2007 - 2013 GreenDelta. This study resulted in the conclusion that the biodiesel production process from WCO in particular is more environmentally sustainable as compared to the preparation of diesel from raw oil, also taking into account the combustion products that are released into the atmosphere as exhaust emissions.

• 한국정밀공학회지
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• 제30권2호
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• pp.137-142
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• 2013

The indiscriminate use of the fossil fuel has caused serious environmental pollutions such as the shortage of energy and global warming. Microalgae have being emphasized as $3^{rd}$ generation biomass which makes the carbon dioxide reduce effectively as well as produces the biofuel. Large scale production of microbial biomass by continuous culture is a quite challenging issue, because off-line optimization strategies of a microbial process utilizing a model-based scheme give rise to many difficult problems. In this paper, the static and simple control method which was able to be applied in time-variant growth environment and large scale of algae culture was studied. The significant disturbances in on-line measurement of cell density were reduced by Savitzky-Golay FIR smoothing filter. Dunaliella salina was cultivated continuously in a flat panel photobioreactor by the on-off control of the turbidostat process.