• Journal of Korean Society on Water Environment
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• v.39 no.1
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• pp.20-29
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• 2023

This study analyzed the relationship between harmful cyanobacterial abundance and environmental factors in order to figure out the causes of the recent increase of cyanobacteria in Lake Dukdong from 2019 to 2021. Lake Dukdong, which is used as a drinking water source for Gyeongju City, has an algae alert system in place. Lake Dukdong has maintained good water quality, but algae alert level 1 (over 1,000 cells/mL) has been issued in recent years. As a result of Pearson correlation analysis (from May to Oct.), the cell density of Microcystis and Aphanizomenon, which form part of the most harmful cyanobacteria genus, were significantly positively correlated with the water temperature and water storage volume. T-test was performed to compare the data from 2016-2018 and 2019-2021 (from May to Oct.). The average density of harmful cyanobacteria cells increased about six-fold from 54 to 344 cells/mL. There were significant differences in water temperature, pH, total nitrogen (TN), total phosphorus (TP), TN/TP ratio, water storage volume, and cyanobacterial cell density. Water temperature increased from 19.2 to 22.8 ℃. TP concentration increased from 0.017 to 0.028 mg/L. The main cause of the recent increase of harmful cyanobacteria in Lake Dukdong is thought to be the increase in water temperature, TP concentration, and water storage volume from 2019 and 2021, resulting in more favorable conditions for cyanobacterial growth.

• Membrane and Water Treatment
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• v.13 no.1
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• pp.15-20
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• 2022

The continuous industrial growth increases the volume of pollutants discharged into the water, which induces Cyanobacteria in the receiving bodies. The removal of various cyanobacteria such as Microcystis, Anabaena, and Oscillatoria was explored to analyze their removal characteristics using different chemical and mineral coagulants. The chemical coagulants, including poly aluminium chloride (PACl), Alum, and mineral coagulants such as Loess and Illite, were tested to remove selected cyanobacteria. Results indicated that the removal rate increased with coagulant dosage regardless of the type of coagulant. The removal of selected cyanobacteria using chemical coagulant was found in the order: Microcystis > Anabaena > Oscillatoria. The PACl coagulant showed the most efficient removal rate for Microcystis, Anabaena, Oscillatoria. Removal rate of Microcystis conducted by PACl showed 92% at 100,000 cells/mL and 98.4% at 1,000,000 cells/mL whereas Illite showed lower 70% and Loess showed lower 50% in both 100,000 cells/mL and 1,000,000 cells/mL. The removal rate of Anabaena and Oscillatoria by PACl and Alum was higher 80%, while the other coagulants exhibited lower than 75% at 1,000,000 cells/mL. The removal rate of Oscillatoria by PACl was 80.1%, while the other coagulants exhibited lower than 70% at 1,000,000 cells/mL. Moreover, the mineral coagulants showed better removal efficiency at a higher concentration than low concentration during experiments. Therefore, removing cyanobacteria from water streams can be improved through coagulation by selecting a specific coagulant for a particular type of algae.

• ALGAE
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• v.38 no.1
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• pp.1-22
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• 2023

An enormous body of research is focused on finding ways to commercialize carotenoids produced by the unicellular green alga, Haematococcus, often without the benefit of a sound phylogenetic assessment. Evidence of cryptic diversity in the genus means that comparing results of pigment studies may be confounded by the absence of a phylogenetic framework. Moreover, previous work has identified unnamed strains that are likely candidates for species status. We reconstructed the phylogeny of an expanded sampling of Haematococcus isolates utilizing data from nuclear ribosomal markers (18S rRNA gene, 26S rRNA gene, internal transcribed spacer [ITS]-1, 5.8S rRNA gene, and ITS-2) and the rbcL gene. In addition, we gathered morphological, ultrastructural and pigment data from key isolates of Haematococcus. Our expanded data and taxon sampling support the concept of a new species, H. privus, found exclusively in North America. Despite overlap in numerous morphological traits, results indicate that ratios of protoplast length to width and akinete diameter may be useful for discriminating Haematococcus lineages. High growth rate and robust astaxanthin yield indicate that H. rubicundus (SAG 34-1c) is worthy of additional scrutiny as a pigment source. With the description of H. privus, the evidence supports the existence of at least five, species-level lineages in the genus. Our phylogenetic assessment provides the tools to frame future pigment investigations of Haematococcus in an updated evolutionary context. In addition, our investigation highlighted open questions regarding polyploidy and sexuality in Haematococcus which demonstrate that much remains to be discovered about this green flagellate.

• ALGAE
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• v.38 no.4
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• pp.283-294
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• 2023

Previous research indicated that free-living sporangial filament keep hollow morph under high-culture density and form bipartite cells under low-culture density, while the following conchospore release was inhibited by high light. Here, we further explored the molecular bases of these affects caused by light and culture density using a transcriptome analysis. Many differentially expressed genes (DEGs) related to carbon dioxide concentration and fixation, photosynthesis, chlorophyll synthesis and nitrogen absorption were upregulated under high-light conditions compared with low-light conditions, indicating the molecular basis of rapid vegetative growth under the former. The stress response- and ion transport-related DEGs, as well as the gene encoding the vacuole formation-brefeldin A-inhibited guanine nucleotide exchange protein (BIG, py05721), were highly expressed under high-density conditions, indicating the molecular basis of the hollow morph of free-living sporangial filaments under high-culture density conditions. Additionally, the brefeldin A treatment indicated that the hollow morph was directly influenced by vacuole formation-related vesicle traffic. Others DEGs related to cell wall components, zinc-finger proteins, ASPO1527, cell cycle and cytoskeleton were highly expressed in the low density with low-light group, which might be related to the formation and release of conchospores. These results provide a deeper understanding of sporangial filaments in Neopyropia yezoensis and related species.

• ALGAE
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• v.39 no.1
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• pp.17-30
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• 2024

Red macroalga Pyropia yezoensis is a high valuable cultivated marine crop. Its acclimation to cold stress is especially important for long cultivation period across winter in coasts of warm temperate zone in East Asia. In this study, the response of P. yezoensis thalli to low temperature was analyzed on physiology and transcriptome level, to explore its acclimation mechanism to cold stress. The results showed that the practical photosynthesis activity (indicated by Φ_PSII and qP) was depressed and pigment allophycocyanin content was decreased during the cold stress of 48 h. However, the F_v/F_m and non-photochemical quenching increased significantly after 24 h, and the average growth rate of thalli also rebounded from 24 to 48 h, indicating a certain extent of acclimation to cold stress. On transcriptionally, the low temperature promoted the expression of differentially expressed genes (DEGs) related to carbohydrate metabolism and energy metabolism, while genes related to photosynthetic system were depressed. The increased expression of DEGs involved in ribosomal biogenesis and lipid metabolism which could accelerate protein synthesis and enhance the degree of fatty acid unsaturation, might help P. yezoensis thallus cells to cope with cold stress. Further co-expression network analysis revealed differential expression trends along with stress time, and corresponding hub genes play important roles in the systemic acquired acclimation to cold stress. This study provides basic mechanisms of P. yezoensis acclimation to cold temperature and may aid in exploration of functional genes for genetic breeding of economic macroalgae.

• Journal of Environmental Science International
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• v.6 no.2
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• pp.141-152
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• 1997

The pilot plant had been made so as to be an association system from the various items managed to have degrees of efficiency and It have been done to consider the experimental result with irradiance period and pH influence of all major things to treatment function of Waste Stabilization Pond. The results are as following. The attained results for continuous & cyclic irradiance 1. 24L.-reactor was prior to 12L.-12D.-reactor on oxygen generation & algal production ability. 2. 24L.-reactor was prior to 12L.-12D.-reactor on nutrients removal efficiency. 3. In 24L.-reactor it maintained 5mg/L∼6mg/L, DO concent enough to a fish's survival. The attained results for pH condition 1. Oxygen generation ^ algal production in pH 4-reactor were higher than those in pH 10-reactor. 2. The acidic condition at pH 4 and alkalic condition at pH 10 did not so much affect an algal growth and nutrients removal. The attained results for whole 1. In view of the results appeared as [(NH3-N)+(NO3-N)] removal efficiency, 89.1%∼93.9% and PO4-P removal efficiency, 34.3%∼83.7% & COD removal efficiency, 88.5%∼93.9%. It is possible to treat the wastewater with starch and pH which have been known as thedifficult problem. 2. At the point of non using methanol to nitrificate NO3-N, the nutrients removal method by using an algal growth is the most economical method in the whole nutrients removal methods. 3. The nutrients removal method by using an algal growth contributes to natural ecosystem. 4. The nutrients removal method by using an algal growth is excellant in the prevention against the eutrophication.

• Journal of Microbiology and Biotechnology
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• v.17 no.3
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• pp.414-419
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• 2007

Botryococcus braunii is a green colonial fresh water microalga and it is recognized as one of the renewable resources for production of liquid hydrocarbons. CFTRI-Bb-l and CFTRI-Bb-2 have been reported for the first time and their performance with regard to growth and biochemical profile is presented here. The present study focused on effect of carbon dioxide $(CO_2)$ on biomass, hydrocarbon, carbohydrate production, fatty acid profile, and carotenoid content in various species of B. braunii (LB-572, SAG 30.81, MCRC-Bb, N-836, CFTRI-Bb-l, and CFTRI-Bb-2) at 0.5, 1.0, and 2.0% (v/v) levels using a two-tier flask. $CO_2$ at 2.0% (v/v) level enhanced growth of the organism, and a two-fold increase in biomass and carotenoid contents was observed in all the B. braunii strains studied compared with control culture (without $CO_2$ supplementation). At 1 % and 2% (v/v) $CO_2$ concentrations, palmitic acid and oleic acid levels increased by 2.5 to 3 folds in one of the strains of B. braunii (LB-572). Hydrocarbon content was found to be above 20% at 2% $CO_2$ level in the B. braunii LB-572, CFTRI-Bb-2, CFTRI-Bb-l, and N-836 strains, whereas it was less than 20% in the SAG 30.81 and MCRC-Bb strains compared with control culture. This culture methodology will provide information on $CO_2$ requirement for growth of algae and metabolite production. B. braunii spp. can be grown at the tested levels of $CO_2$ concentration without much influence on culture pH.

• Korean Journal of Environmental Biology
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• v.21 no.2
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• pp.216-221
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• 2003

Various physico-chemical and biological methods have been used to remove. cyanobacteria which causes blooms and releases toxin. The purpose of the following experiment is aimed finding out which cyanobacteria are affected by $_L-lysine $ and what concentration of$_L-lysine $ inhibits cyanobacteria. The 20 samples of Microcystis sp. have been tested. To prove the growth inhibition on Microcystis sp., double-layered agar method and microplate method have been used. When the concentration of $_L-lysine $ is as heavy as 100 ${\mu}g\; ml^{-1}$~300 ${\mu}g\; ml^{-1}$, some Microcystis sp. have made halo zone. Some Microcystis sp. have shown so high activity as to be inhibited in their growth by the $_{L}$-lysine of concentration 10 ${\mu}g\; ml^{-1}$ with microplate method. These activities are various in accordance with every species. In additions, the microplate method has been proven to be an easy way which examine the lytic activity on the species of algae.e.

• Journal of Korean Society of Water and Wastewater
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• v.28 no.5
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• pp.555-560
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• 2014

This study investigates the effect of sodium bicarbonate ($NaHCO_3$) on growth of S.dimorphus. $NaHCO_3$ concentration was varied from 0 to 2 g-C/L. As a result, the increase in concentration of $NaHCO_3$ up to 1.5 g-C/L increased dry weight of algae. The highest specific growth rate of S. dimorphus was $0.36day^{-1}$ which was obtained at concentration of 0.5 g-C/L $NaHCO_3$. pH showed a large variation range at the concentrations lower than 0.5 g-C/L $NaHCO_3$ whereas inorganic carbon, nitrate and phosphorus removal rates were almost same at the concentrations higher than 0.5 g-C/L $NaHCO_3$ (0.75, 1, 1.25, 1.5, 2 g-C/L $NaHCO_3$). Their average inorganic carbon, nitrate and phosphorus removal rate were 70 mg-C/L/d, 11.3 mg-N/L/d, and 1.6 mg-P/L/d, respectively. Thus, $NaHCO_3$ didn't effect on inorganic carbon, nitrate and phosphorus removal rate of S. dimorphus.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.2
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• pp.117-122
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• 1991

Microbial organisms including yeast, Acinetobacter sp. AG-3, Chlorococcum sp., Chlorella sp. and some of their combinations were tested to evaluate growth efficiency of Tigriopus japonicus. Body length and weight of the copepoda were measured during four days experiment. Acinetobacter sp. AG-3, dried yeast(produced Wago), Chlorella sp., Chlorococcum sp. and mixed culture were used as food sources. Yeast(Y.) was the most effective food for the growth during nauplius stage and efficiencies of bacteria(Bact.)+chlorococcum sp.(RA), Chlorococcum sp.(RA), Bact.+chlorella sp.(Ch.), Bact. and Ch. decreased in odor, while for the growth of copepodite and adult, Bact.+RA was the most effective food with decreased efficiency of Y., RA, Bact. + Ch., Bact., Ch. in order. The ratio of weight gain to the food uptaken, after the weight and food units were converted to carbon, was between 21.6 and $68.7\%$, This result suggests that some kinds of bacteria, algae and mixed cultural microorganisms could be good food sources for the growth of copepoda.