• Title/Summary/Keyword: agriculture production

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Initiation, proliferation, and improvement of a micropropagation system for mass clonal production of banana through shoot-tip culture

  • Khaskheli, Allah Jurio;Ali, Muharam;Shah, Syad Zakir Hussain;Memon, Zohra Fatima;Awan, Saleem;Khaskheli, Muhammad Ibrahim;Khaskheli, Mohsin Ali;Magsi, Bilqees;Qambrani, Zareen;Khaskheli, Asad Ali
    • Journal of Plant Biotechnology
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    • v.48 no.2
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    • pp.86-92
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    • 2021
  • The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%).

Determinants of Participation in Environment-Friendly Agricultural Production Organization - Focusing on Chungnam Area - (친환경농업 생산조직 참여결정 요인분석 - 충남지역을 중심으로 -)

  • Seo, Hwan-Seok;Hwang, Jae-Hyun
    • Korean Journal of Organic Agriculture
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    • v.27 no.3
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    • pp.271-288
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    • 2019
  • It has continuously promoted to scale up the agricultural management body in order to cope with the market-opening pressure such as the UR negotiation, the DDA negotiation, and more conclusions of FTAs and to enhance the competitiveness of our agriculture. This study evaluated the factors associated with production organization participation centered on environmentally-friendly agriculture farmers in order to improve the production efficiency of environmentally-friendly agriculture in the aspect of production. In order to achieve this objective, this study evaluated the factors that contributed to participating in an organization using the bivariate probit model focusing on environmentally-friendly agriculture farmers in Chungnam. This study examined the validity and reliability of the study model through exploratory factor analysis. The results of factor analyses identified four factors: "distribution", "production", "organization operation", and "social capital". The results of the bivariate probit model showed that distribution significantly affected production activities and distribution, organization operation, agricultural income, and gender significantly influenced sale activities. Environmentally-friendly production organization had not been able to provide the productive support that was needed for local farmers to produce environmentally-friendly agricultural products in a region. Additionally, farming education did not provide the information needed by the farmers. Although producers had a network within a production organization, it was operated mainly for administrators. Therefore, participants felt somewhat lacking. This study identified the level of organization of producer organizations in Chungnam area, where environmentally-friendly agriculture is well activated. This study suggested implications for future production organization participation by evaluating factors affecting participation quantitatively.

Comparison of In vitro Gas Production, Metabolizable Energy, Organic Matter Digestibility and Microbial Protein Production of Some Legume Hays

  • Karabulut, Ali;Canbolat, Onder;Kalkan, Hatice;Gurbuzol, Fatmagul;Sucu, Ekin;Filya, Ismail
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.4
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    • pp.517-522
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    • 2007
  • The aim of this study was to compare in vitro gas production kinetics, metabolizable energy (ME), organic matter digestibility (OMD) and microbial protein (MP) production of widely used legume hays in ruminant nutrition in Turkey. Gas production were determined at 0, 3, 6, 12, 24, 48, 72 and 96 h and their kinetics were described using the equation p = a+b ($1-e^{-ct}$). There were significant differences among legume hays in terms of chemical composition. The crude protein content of legume hays ranged from 11.7 to 18.6% of dry matter (DM); crude fat from 2.1 to 3.5% DM; neutral detergent fiber from 35.6 to 52.0% DM; acid detergent fiber from 32.0 to 35.5% DM and acid detergent lignin 1.7 to 11.0% DM. Total gas production after 96 h incubation ranged between 61.67 and 76.00 ml/0.200 g of substrate. At 24, 72 and 96 h incubation the total gas production for common vetch were significantly (p<0.01) higher than those of the other legume hays. The ME, OMD and MP of legume hays ranged from 9.09 to 11.12 MJ/kg DM, 61.30 to 75.54% and 90.35 to 138.05 g/kg DM, respectively. The ME, OMD and MP of common vetch was significantly (p<0.01) higher than those of the other hays due to low cell-wall contents and high crude protein. At the end of the experiment, differences in chemical composition of legume hays resulted in the differences in the in vitro gas production, gas production kinetics and the estimated parameters such as ME, OMD and MP. Common vetch can be recommended to hay producers and ruminant breeders, due to high ME, OMD and MP production.

The Role of Phosphatidylinositol 3-kinase and Mitogenic Activated Protein Kinase on the Differentiation of Ovine Preadipocytes

  • Choi, K.C.;Shrestha, S.G.;Roh, S.G.;Hishikawa, D.;Kuno, M.;Tsuzuki, H.;Hong, Y.H.;Sasaki, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.8
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    • pp.1199-1204
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    • 2003
  • The aim of this study was to investigate the role of phosphatidylinositol 3-kinase (PI3 kinase) and the mitogenactivating protein (MAP) kinase pathway on the differentiation of ovine preadipocytes. In order to investigate this issue, we monitored glycerol 3-phosphate dehydrogenase (GPDH) activity during differentiation with specific inhibitors of PI3 kinase and MAP kinase-Erk kinase, LY294002 and PD098059, respectively. The preadipocytes, which were obtained from ovine subcutaneous adipose tissues, were proliferated to confluence and then differentiated to adipocytes in differentiation medium with each inhibitor for 10 days. The confluent preadipocytes and differentiated adipocytes at days 3, 7 and 10 were harvested for assay of GPDH activity. LY294002 inhibited the differentiation program in dose- and day-dependent manners during 10 days of differentiation. PD098059 did not affect GPDH activity during differentiation. Furthermore, the expression of peroxisome proliferator-activated receptor ${\gamma}2$ (PPAR-${\gamma}2$), the representative early gene of differentiation, was markedly reduced by LY294002 treatment, although PD098059 did not change it. Our results demonstrated that the activation of PI3 kinase contributes to the differentiation process of ovine preadipocytes.

Effect of propolis supplementation and breed on growth performance, immunity, blood parameters and cecal microbiota in growing rabbits

  • Al-Homidan, Ibrahim;Fathi, Moataz;Abdelsalam, Magdy;Ebeid, Tarek;Abou-Emera, Osama;Mostafa, Mohamed;Abd El-Razik, Mohamed;Shehab-El-Deen, Mohamed
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1606-1615
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    • 2022
  • Objective: The present study was conducted to investigate the potential effects of dietary supplemented propolis in two growing rabbit breeds on growth performance, immune response, blood parameters, carcass characteristics, and cecal microflora composition. Methods: A total of 90 growing rabbits aged 6 weeks from two breeds (V-line and Jabali) were randomly allocated to 3 dietary propolis experimental treatments. The experimental treatments consisted of a 2×3 factorial arrangement with two rabbit breeds and three levels of dietary propolis supplementation (0, 250 mg/kg, and 500 mg/kg). Each sub-treatment has 15 rabbits. The experimental period lasted six weeks. Results: There were no significant differences in growth performance and carcass characteristics due to propolis administration. Propolis supplementation at a high level significantly increased (linear; p<0.05) cellular-mediated immunity compared with the unsupplemented group. Furthermore, the rabbits receiving propolis exhibited a significant increase (linear and quadratic; p<0.03) in IgM immunoglobulins compared to the control. The current study provides further evidence that the dietary inclusion of propolis can significantly reduce pathogenic bacterial colonization in growing rabbits. The total count of microflora, E. coli, and Salmonella spp. was significantly lower (linear; p<0.01) in supplemented rabbit groups compared to the control group according to the microbiological analysis of cecal digesta. Based on breed effect, the results indicated that Jabali rabbits (local) performed better than V-line rabbits (foreign) in the majority of the studied traits. Conclusion: Dietary propolis is promising for further investigation into improving intestinal health and enhancing immunity in growing rabbits.

Cross-Specific Amplification of SSR Markers in Dalbergia latifolia and Dalbergia sissoo - A Case for Hybrid Seed Production

  • Asif Javed Muhammad;Izhar-Ul-Haq;Nauman Ghafoor;Fazal-Ur-Rehman;Atif Ali;Zia Bilal Muhammad
    • Journal of Forest and Environmental Science
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    • v.40 no.3
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    • pp.259-264
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    • 2024
  • Dalbergia sissoo and Dalbergia latifolia are the two most important timber wood species that are indigenous to the Indo-Pak subcontinent producing very high-quality timber. Its wood is used to produce high-quality furniture. Due to overexploitation and dieback disease, their production is seriously hampered and threatens their genetic diversity. Several ecotypes are tolerant to dieback thus offering an opportunity to develop a superior hybrid for the establishment of plantations for hybrid seed production. Hybrid evaluation can only be done by using DNA markers such as SSR markers. Cross-specific amplification of SSR markers is a cost-effective way of producing DNA markers for species lacking genetic information. Here, we report for the first-time successful cross-amplification of SSR markers in Dalbergia latifolia and added new SSR markers in D. sissoo. Cross-species amplification resulted in 13 successful SSR markers in D. latifolia and an addition of 14 markers in D. sissoo of expected sizes. Six SSR markers were further selected randomly to validate the breeding behavior of both species. A diverse DNA profile of seed progenies matched to different pollen donors deviated from the same mother suggested cross-pollination is the most likely mechanism of seed production in D. sissoo and D. latifolia. However, the results must be validated by using a large sample size and through controlled pollinations. SSR markers thus developed will be useful in the conservation and development of superior hybrids for sustainable development and production of commercial populations in Dalbergia sissoo and D. latifolia.

A Research Review of Village Chicken Production Constraints and Opportunities in Zimbabwe

  • Mapiye, C.;Mwale, M.;Mupangwa, J.F.;Chimonyo, M.;Foti, R.;Mutenje, M.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.11
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    • pp.1680-1688
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    • 2008
  • Development of village chicken production can be a sustainable way of helping to meet the welfare needs of rural populations and raise their living standards. There is a dearth of information on research conducted to characterize, understand and develop the village chicken production systems in Zimbabwe. This review focuses on constraints, opportunities and research needs for the improvement of village chicken productivity in Zimbabwe. Village chicken production in Zimbabwe is extensive and dominated by indigenous chickens that exhibit remarkable adaptation to local environments. The multitude functions of village chickens, which include the provision of high quality protein meat and eggs, cash through sales and socio-cultural roles, are discussed in detail. Human gender aspects in village chicken production are highlighted. The factors that hamper village chicken productivity are reviewed together with opportunities and research needs. The major constraints include shortage of feed, poor health and housing management. Any improvements in these constraints may lead to sustainable increase in village chicken productivity.