• 제목/요약/키워드: agricultural science

검색결과 22,965건 처리시간 0.046초

Tectella patellaris from Korea

  • Seok, Soon-Ja;Jung, Yun-A;Jin, Yong-Ju;Park, In-Cheol;Kim, Wan-Gyu;Kim, Yang-Sup;Yoo, Kwan-Hee
    • Mycobiology
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    • 제39권4호
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    • pp.303-305
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    • 2011
  • One rare and interesting species collected from Gyeryong-san, Chungnam Province is described and illustrated in detail. The species "Tectella patellaris (Fr.) Murr." and genus "Tectella Earle" is a first record for Korean fungal flora. Specimens cited here have been deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

Production of rice transformant with useful gene

  • Suh, Suhk-Cheol;Lee, Seung-In;Lim, Myung-Ho;Lee, Si-Myung;Kim, Dong-Hern;Eun, Moo-Young;Kim, Ho-Il
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2001년도 추계학술발표대회
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    • pp.53-55
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    • 2001
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Production of Colored Cocoons by Feeding Dye-Added Artificial Diet

  • Kang, Pil-Don;Kim, Mi-Ja;Jung, I-Yeon;Kim, Kee-Young;Kim, Young-Soon;Sung, Gyoo-Byung;Sohn, Bong-Hee
    • International Journal of Industrial Entomology and Biomaterials
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    • 제22권1호
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    • pp.21-23
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    • 2011
  • In order to produce the colored cocoons we finalized the adequate amount of dyes throughout several trial and error experiments. The proper amount of each dye required for per 100 g of each colored cocoon turned out to be 150 mg of Rhodamine, 1560 mg of Thionin, 170 mg of Neutral red, and 200 mg of N-Blue, respectively. With this amount of dyes silkworms grew without physiological disorders. In order to produce colored cocoons, artificial diet composed mainly of mulberry leaves was fed to silkworms from the beginning of 5th instar, and subsequently fed with dye included diet from $4^{th}$ day of the 5th instar. This process resultantly produced colored silkworm body from the onset of feeding and subsequently colored cocoons and eggs. Nevertheless, the dye induced color was not inherited to next generation.

Expression of Recombinant Human Stem Cell Factor (hSCF) Protein using Bombyx mori Protein Disulfide Isomerase (bPDI)

  • Kim, Sung-Wan;Yun, Eun-Young;Kim, Seong-Ryul;Park, Seung-Won;Kang, Seok-Woo;Lee, Kwang-Gill;Kwon, O-Yu;Goo, Tae-Won
    • International Journal of Industrial Entomology and Biomaterials
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    • 제21권2호
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    • pp.151-155
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    • 2010
  • Protein disulfide isomerase (PDI) catalyzes the oxidation of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have developed expression system using the Bombyx mori PDI (bPDI) as a fusion partner. bPDI gene fusion was found to improve the production of recombinant hSCFs. Thus, we conclude that bPDI gene fusion will be very useful for the large-scale production of biologically active recombinant proteins.

An artificial diet for the swallowtail butterfly, Papilio xuthus

  • Kim, Seonghyun;Hong, Seongjin;Park, Haechul;Lee, Youngbo;Park, Kwanho;Choi, Wonho;Kim, Namjung
    • International Journal of Industrial Entomology and Biomaterials
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    • 제28권1호
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    • pp.1-4
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    • 2014
  • The effect of an artificial diet on developmental rate, a life history parameter, was examined for the swallowtail butterfly Papilio xuthus. Artificial insect diets are an essential component of many insect rearing systems that produce insects for research purposes. Complex agar-gelled diets are generally prepared in large batches and used shortly after preparation because the degradation of perishable diet ingredients, such as vitamins and fatty acids, can adversely affect insect quality (Brewer 1984). However, the timing of diet preparation may be inconvenient, and large batches wasteful, if the unused excess is discarded. The percentage of pupation varied considerably, with no significant differences among diets, on which a maximum pupation percentage of 83% was observed. Pellet-type diets were investigated with the aim of developing a more easily prepared diet. The extrusion of the artificial diet under high temperature and pressure may induce desirable chemical and physical changes in the extruded product. The purpose of the present study was to develop an artificial diet for rearing P. xuthus.

Microsatellite analysis of 20 mulberry varieties preserved in Korea

  • Chan Young, Jeong;Sang Kuk, Kang;Nam-Suk, Kim;Ik Seob, Cha;Seong-Wan, Kim;Jong Woo, Park;Kee-Young, Kim
    • International Journal of Industrial Entomology and Biomaterials
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    • 제45권2호
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    • pp.49-55
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    • 2022
  • A total of 20 mulberry varieties preserved in Korea were typed for eight polymorphic microsatellite loci. We obtained 6 to 15 alleles per locus with an average value of 10.6, per-locus observed heterozygosity ranging from 0.35 to 1.00, and per-locus polymorphic information content (PIC) ranging from 0.61 to 0.87, indicating that most loci are highly variable. Phylogenetic analysis using the eight microsatellite loci was sufficiently suitable for classifying 20 mulberry varieties preserved in Korea. A total of 160 variety-specific apomorphic alleles were obtained from eight loci discriminated 20 mulberry varieties. These variety-specific alleles from this analysis are expected to be useful for the discrimination of other mulberry varieties. Furthermore, a substantial number of homozygote loci, represented by 60 among 180 alleles in eight loci were found. These results collectively suggest that these microsatellite locus primers are potentially crucial molecular markers for the eventual classification of mulberry varieties that are preserved as hundreds in Korea.