• 제목/요약/키워드: agricultural science

검색결과 22,965건 처리시간 0.052초

Overproduction of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) confers resistance to the herbicide glyphosate in transgenic rice

  • Lee, Soo-In;Kim, Hyun-Uk;Shin, Dong-Jin;Kim, Jin-A;Hong, Joon-Ki;Kim, Young-Mi;Lee, Yeon-Hee;Koo, Bon-Sung;Kwon, Sun-Jong;Suh, Seok-Chul
    • Journal of Plant Biotechnology
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    • 제38권4호
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    • pp.272-277
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    • 2011
  • Plants expressing Agrobacterium sp. strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant EPSPS. In order to develop herbicide-resistant rice, we prepared transgenic rice plants with CP4 EPSPS gene under the control of CaMV 35S promoter for over-expression. A recombinant plasmid was transformed into rice via Agrobacterium-mediated transformation. A large number of transgenic rice plants were obtained with glyphosate and most of the transformants showed fertile. The integration and expression of CP4 EPSPS gene from regenerated plants was analyzed by Southern and northern blot analysis. The transgenic rice plants had CP4 EPSPS enzyme activity levels more than 15-fold higher than the wild-type plants. EPSPS enzyme activity of transgenic rice plants was also identified by strip-test method. Field trial of transgenic rice plants further confirmed that they can be selectively survived at 100% by spay of glyphosate (Roundup$^{(R)}$) at a regular dose used for conventional rice weed control.

누에번데기 유래 불포화지방산을 이용한 기능성 효과 시험 (Study on the Unsaturated Fatty Acids in the Pupae of Silkworm, Bombyx mori)

  • 강필돈;김진원;정이연;김기영;강석우;김미자;류강선
    • 한국잠사곤충학회지
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    • 제48권1호
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    • pp.21-24
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    • 2006
  • 누에유래 불포화지방산의 분리법을 확립하여 특허출원 하였다(출원번호 :2004-76364호). 분리 정제된 누에유래 불포화지방산의 비율은 70% 이상이었다. 누에유래 불포화지방산의 기능성을 시험해본 결과, 고지혈증 개선의 효과를 보였고, 피부단백질인 콜라겐 생성을 유의적으로 증가시키며, 콜라겐 분해효소 활성을 유의적으로 억제하였다. 또한, 피부건조증 개선에 빠르고 탁월한 효과가 있었다.

Species Belonging to Genus Protodaedalea in Korea

  • Seok, Soon-Ja;Jung, Yun-A;Jin, Yong-Ju;Park, In-Cheol;Kwon, Soon-Woo;Kim, Yang-Sup;Yoo, Kwan-Hee
    • Mycobiology
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    • 제39권4호
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    • pp.301-302
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    • 2011
  • One rare and interesting species collected from Seorak-san, Inje-gun; Yeonyeop-san, Hongcheon-gun; Daeam-san, Yanggugun, Gangwon-do; Pocheon-gun, Gyeonggi-do; Songni-san, Boeun-gun; Joryeong-san, Goesan-gun, Chungcheongbuk-do and Sobaeksan, Yeongju-si, Gyeongsangbuk-do is described and illustrated in detail. The species "Protodaedalea hispida Imazeki" and genus "Protodaedalea Iamzeki" has not been previously recorded in Korean fungal flora. The specimens have been deposited in the Herbarium Conservation Center of the National Academy of Agricultural Sciences.