• 제목/요약/키워드: agricultural research

검색결과 15,246건 처리시간 0.047초

Heterologous Expression and Characterization of a Laccase from Laccaria bicolor in Pichia pastoris and Arabidopsis thaliana

  • Wang, Bo;Yan, Ying;Xu, Jing;Fu, Xiaoyan;Han, Hongjuan;Gao, Jianjie;Li, Zhenjun;Wang, Lijuan;Tian, Yongsheng;Peng, Rihe;Yao, Quanhong
    • Journal of Microbiology and Biotechnology
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    • 제28권12호
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    • pp.2057-2063
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    • 2018

  • Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and $40^{\circ}C$. It exhibited $K_m$ and $V_{max}$ values of $0.565mmol\;l^{-1}$ and $1.51{\mu}mol\;l^{-1}\;min^{-1}$ for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.

  • https://doi.org/10.4014/jmb.1807.08042 인용 PDF KSCI

Studies on the suppression of transmission of anthracnose with covering method and environment friendly agricultural materials (EFAM) in pepper field

  • Kang, B.R.;Ko, S.J.;Kim, D.I.;Choi, D.S.;Kim, J.D.;Choi, K.J.
    • 한국유기농업학회지
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    • 제19권spc호
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    • pp.291-294
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    • 2011

  • We studies a model for management of pepper anthracnose based covering method and spraying system in field. 1. Among 82 organic fungicides, 42 materials showed most effective inhibition against mycelia growth of the Colletotrichum acutatum in vitro. 23 formulated biocontrol agents were chosen to control the disease from 42 biocontrol agents in greenhouse. In the end, five kinds (2 plant extracts, 2 biopesiticides, 1 Bordeaux mixture) were selected from 23 materials in the field. 2. The mulching materials of bed covering in fruit season were thin non-woven fabric sheet and black plastic. The use of a fabric sheet was reduced the spread of anthracnose as compared to the plastic covering. 3. The application with the chosen materials was reduced 34% of anthracnose for 7 times sprays to planting 70 days as compared to the untreated control. In yield, nonwoven fabric sheet with formulated biopesticides was increased 17% than black plastic. 4. This result indicated that the developed biocontrol strategy could be an effective and economic crop protection system in organic pepper cultivation field.

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HEATING PERFORMANCE OF AIR SOURCE HEAT PUMP WITH HEAT REGENERATIVE DEVICE USING FIBER BELT

  • Ryou, Y.S.;Chang, J.T.;Kim, Y.J.;Kang, G.C.;Yun, J.H.;Lee, K.J.
    • 한국농업기계학회:학술대회논문집
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    • 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.III
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    • pp.647-653
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    • 2000

  • In this research the heat regenerative technology was employed to eliminate frosting on evaporator coil and improve COP of the heat pump system. This heat regenerative device(HRD) has very simple structure consisting a geared motor and a porous fiber belt passing through alternatively between cold and warm air duct. The laboratory test showed that the heat pump system with HRD yielded an impressive COP higher than 3.5 at the outside air temperature of $-7^{circ}C$ in heating mode.

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공공기관 농업연구의 발전 방향 (Policy Recommendations on the Development of Agricultural Research of Public Agricultural Institution)

  • 임형백
    • 농촌지도와개발
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    • 제15권2호
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    • pp.293-323
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    • 2008

  • The purpose of this study is to suggest some policy recommendations on the development of agricultural research of public agricultural institution. Critics say public agricultural institution are not working fast enough. As for efficiency itself, it seems reasonable to conclude that privatization plan is the best plan. The change that is needed to improve the efficiency of public agricultural institution is not the point in question. I will concentrate on the extraordinary nature of agriculture. We must not forget the important of extraordinary nature of agriculture. What is important is to have a genuine debate on extraordinary nature of agriculture and agricultural research. My aim is to highlight some aspects of agricultural research of public institution. Most important thing is, agricultural research is a valuable resource for nation. As I mentioned before, the change that is needed to improve the efficiency of public agricultural institution is not the point in question But there is much room for consideration for proper method. Impatient privatization without serious consideration could have a negative impact on the agricultural research.

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