• The Plant Pathology Journal
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• 제39권2호
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• pp.234-234
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• 2023

• The Plant Pathology Journal
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• 제35권1호
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2016년도 추계 학술발표논문집
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• pp.156-160
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• 2016

• The Plant Pathology Journal
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• 제37권5호
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• pp.437-445
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• 2021

Tilletia laevis Kuhn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron microscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What's more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breeding of common bunt resistance.

• The Plant Pathology Journal
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• 제35권3호
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• pp.208-218
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• 2019

Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

• 한국응용곤충학회지
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• 제17권1호
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• pp.1-5
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• 1978

필자들은 우리나라에서 발생되고 있는 콩해충 중 나방류해충을 대상으로 조사한바 총 49종이 채집 분류되었으므로 그 목록을 발표한다. 또한 그들은 가해형태별로 대별하여 콩립 및 꼬투리를 가해하는 종류, 잎을 말고 가해하는 종류, 잎을 식해하는 종류 그리고 토양에 서식하며 가해하는 종류등으로 구분하고 각 무리의 중요종들에 대하여 개략적인 생활사 및 피해상황 등을 기술하였다.

• 한국환경과학회지
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• 제30권12호
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• pp.993-1003
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• 2021

It is known that the effect of traditional agricultural techniques of burning farmland such as paddy fields and fields gradually declines and affects both the fauna and flora of the rice paddy as well as pests. Therefore, in this study, a study was conducted to investigate the effects of burning rice paddy fields and rice paddy fields levee on the control effect of winter pests inhabiting agricultural land and the amount of pests generated and damaged during the growing season. As a result of this study, the pest control effect of incineration reduces not only the density of pests, but also beneficial insects (natural enemies) and non-reptiles. It is judged that burning has a very low insect control effect. It is expected to be used as basic data to create a sustainable agricultural environment, such as minimizing various negative effects such as pest control effects, wildfires, and air pollution caused by incineration, and suppressing unnecessary incineration and fine dust generation.

• 한국응용곤충학회지
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• 제55권4호
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• pp.431-437
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• 2016

시설딸기에 발생하는 해충 종류와 피해를 구명하기 위하여 2013년부터 2016년까지 제주지역의 농가포장에서 조사한 결과 8목 31종이 발생되고 있었다. 그중에서 발생과 피해가 큰 해충은 점박이응애(Tetranychus urticae), 꽃노랑총채벌레(Frankliniella occidentalis), 목화진딧물(Aphis gossypii) 3종이었다. 그 외에 딸기가루이와 괴테애매미충은 일부 조사포장에서 피해가 나타났으나 발생과 피해가 점차 증가하고 있었다. 나방류 중에서는 담배거세미나방(Spodoptera litura)의 발생빈도와 피해가 가장 컸으며, 왕담배나방(Helicoverpa armigera)은 조사기간 중 발생빈도는 적었으나 피해는 심했다. 조사 해충 중 황색끈끈이트랩으로 발생밀도를 예찰할 수 있는 꽃노랑총채벌레와 목화진딧물 유시충, 딸기가루이, 괴테애매미충에 대한 시기별 발생특성에 대하여 고찰하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.97-99
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• 2001

Seven vegetables were planted in green houses to compare their yields and damages by insect pests based on three treatments, green music (GM), ultra sonic (US) and control. The vegetable yields of GM treatment were increased by an average of 17.81% than that of control for two years. There was a remarkable statistic difference in the yield between GM and respective controls such as radish, young radish, cucumber, spinach and Brassica oleracea var. acephala. There was no notable yield difference between US and controls for the semen vegetables within two years. The population densities of the insect pests in GM and US treatments were lower than controls. Only a few insect pests of some vegetables in GM and US treatments injured more seriously than in control.

• The Plant Pathology Journal
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• 제38권6호
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• pp.679-684
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• 2022

Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M. oryzae, the direct extraction of M. oryzae genomic DNA from infected rice tissue would be useful alternative for rapid monitoring of changes of avirulence genes without isolation and cultivation of the pathogen. In this study, a fast, low-cost and reliable method for DNA preparation of M. oryzae from a small piece of infected single rice leaf or neck lesion was established. This single step method only required 10 min for DNA preparation and conventional chemical reagents commonly found in the laboratory. The AvrPik and AvrPi9 genes were successfully amplified with the prepared DNA. The expected DNA fragments from 570 bp to 1,139 bp could be amplified even three months after DNA preparation. This method was also suitable for DNA preparation from M. oryzae strains stored on the filter paper. All together these results indicate that the DNA preparation method established in this study is reliable, and could meet the basic needs for polymerase chain reaction-based analysis of M. oryzae.