• Journal of Soil and Groundwater Environment
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• v.20 no.7
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• pp.80-89
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• 2015

Nitrate is on the most seriou pollutant encountered in shallow groundwater aquifer in agricultural area. There are various remediation technologies such as ion exchange, reverse osmosis, and biological denitrification to recover from nitrate contamination. Biological denitrification by indigenous microorganism of the technologies has been reviewed and applied on nitrate contaminated groundwater. In this work, we selected the site where the annual nitrate (NO₃⁻) concentration is over 105 mg/L and evaluated denitrification process with sampled soil and groundwater from 3 monitoring wells (MW4, 5, 6). In the results, the nitrate degradation rate in each well (MW 4, 5, and 6) was 25 NO₃⁻ mg/L/day, 6 NO₃⁻ mg/L/day, and 3.4 NO₃⁻ mg/L/day, respectively. Nitrate degradation rate was higher in batch system treated with 2 times higher fumarate as carbon source than control batch system (0.42M fumrate/1M NO₃⁻), comparing with batch system with soil sample. This result indicates that increase of carbon source is more efficient to enhance denitrification rate than addition of soil sample to increase microbial dynamics. In this work, we also confirmed that monitoring method of functional genes (nirK and nosZ) involved in denitrification process can be applied to evaluated denitrifcation process possibility before application of field process such as in-situ denitrification by push-pull test.

• Journal of Environmental Health Sciences
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• v.34 no.1
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• pp.86-94
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• 2008

Lake Shiwha, an artificial lake located near metropolitan Seoul, offers a unique water environment and has been suspected to have high levels of chemical and microbiological contaminations. Lake Shiwha was originally connected to the sea but currently has four major surface water inputs from agricultural, municipal, industrial areas and in addition an occasional inflow from the sea. The objectives of this study are to investigate the relative contribution of microbial contaminants from each of the inflowing surface waters and to identify appropriate microbial indicator organisms in this unique water environment. We measured the levels of microbial contaminations in the four inflowing surface waters. A number of microbial indicator organisms including total coliform (TC), fecal coliform (FC), E. coli, Enterococci, somatic and male-specific coliphages were analyzed. Bacterial indicator microorganisms were detected and quantified by the $Colilert^{(R)},\;Enterolert^{(R)}$ kit. Surface water (50 l) was sampled by $ViroCap^{TM}\;5"$ cartridge filters and analyzed by the single agar layer method for detecting coliphages. The concentrations of TC, FC, E. coli, and Enterococci were 1543 CFU/100 ml${\sim}1.99{\times}10^6$ CFU/100 ml, 0 CFU/100 ml${\sim}202$ CFU/100ml, 0 CFU/100 ml${\sim}1.80{\sim}10^5$ CFU/100ml, 74 CFU/100 ml${\sim}3408$ CFU/100 ml, respectively. The male-specific and somatic coliphages were detected in three different inflowing surface waters. Isolated E. coli and Enterococci strains were further analyzed by 16s rDNA amplification and subsequent phylogenetic analysis from Jungwang-chun, Ansan-chun, Banwol-chun and penstock of inflowing surface water. Our results indicated that the concentrations of different fecal indicator microorganisms might not be highly correlated with each other. Multiple microbial indicator organisms should be used for monitoring microbial contamination and microbial source tracking methods.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.4
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• pp.340-360
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• 1982

Because of the continuing rapid increase in pesticide usage in Taiwan, much attention has been focus on pesticide contamination of food and effect of pesticides on human and environmental health. The Plant Protection Center (PPC) conducts safety evaluation of pesticides usee! in Taiwan. The pesticides are classified into different groups based on their acute toxicities. Pesticides which are classified into extremely toxic group are not allow to used on short term crops or the continuously harvest crops. The acute toxicity of pesticides to the beneficial insects are also studied, special attention has been paid to the two predators of rice brown planthopper. 60% of cultivated land in Taiwan are paddy field; therefore, acute fish toxicity was taken into consideration when a pesticide was applied for registration to be used in the paddy. Fish toxicities were evaluated by the dangerous rating value which is the amount of pesticide residue in the field water over the TLM value. Mutagenicity of pesticides was continuously evaluated by using Arne's microbial testing method. Island wide survey of residual levels of pesticides of known pollutants such as chlorinated hydrocarbon . insecticides, mercurial compounds in soil, water and biological samples were carried out constantly. The potential of a new1y Imported esticides to pollute the environment were studied by using model ecosystem. Ecological magnification (EM) of a chemical was calculated from model ecosystem. A chemical was considered as a pollutant when its EM value over 5000. In order to ensure the levels of pesticides residue of the crop within the safety limit. The 'tolerance' of pesticides on different crop groupings were established base on 1) acceptable daily intake value of individual pesticides, 2) average daily consumption of each crop groupings by Chinese person, 3) Actual residues of pesticides. on different crops obtained from supervised trials. Total about 79 pesticides for which the tolerances have been established on different crop groupings. Because the intensive agricultural system was adopted in Taiwan. The phytotoxicity of pesticides to the non-target crops was therefore become one of the important factor in the safety evaluation of pesticide usage. These will include 1) direct injury, 2) injury caused by pesticide polluted irrigation water, 3) injury caused by the pesticide polluted soil, 4) reduction of growth caused by the effect of pesticide on the soil microorganisms. This paper will reviewed all the aspects mentioned in the previous .paragraphs. Most the works have done in Taiwan by the PPC.y the PPC.

• Journal of Soil and Groundwater Environment
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• v.9 no.2
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• pp.54-63
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• 2004

For the purpose of finding out the distributions of groundwater uses, the effect of facilities on the parameter and the correlations among measurements, various statistical analysis were carried out with the data of groundwater quality measurements from January to December in 2002. (1) The rates of groundwater for drinking water were 10.5% in Yungcheon-Gu, 10.2% in Kangdong-Gu, and 9.9% in Eunpyung-Gu. The rates of other uses of groundwater were shown to be 58.1 %(786 wells) for civil defense emergency, 22.1% (299 wells) for contamination-concerning, 9.8%(133 wells) for water quality monitoring, consisting of 90% of all groundwater. (2) The 52.6% of groundwater for drinking were demonstrated to be appropriate while 91.9% for other uses-domestic, industrial, agricultural uses- were shown to be proper. (3) For drinking water, the maximum values of colar, turbidity, NH3-N, F, and Fe were 766.9 degree, 69.16NTU, 860.0 mg/l, 5.6 mg/l and 49.87 mg/l respectively. (4) Comparision of skewness and kurtosis for Seoul groundwater, pH was found to be 0.022 and -0.524, but the T.colony, color, turbidity, NH$_3$-N, NO$_3$-N, Fe and Mn respectively fumed out to be 11.641 and 174.324, 8.501 and 80.260, 5.675 and 32.821, 19.507 and 380.994, 3.323 and 17.436, 10.544 and 134.093 and 5.979 and 39.124. (5) In cases of drinking water wells for emergency, the results of statistical analysis showed that building year of the wells, depth and pumping rate didn't affect on whether it was proper for that use or not. It were shown that there were linear correlations between depth and NO3-N(-0.171) and F(0.332) while the correlation coefficients were 0.381 and -0.169 between the building year of well and depth and pumping rate respectively.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.11 no.1
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• pp.63-68
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• 2013

To see if slaked lime and organic fertilizer applications to soil are useful as countermeasures for reducing $^{99}Tc$ concentrations in rice seeds after $^{99}Tc$ contamination of paddy fields, pot experiments were performed for two different paddy soils in a greenhouse. The upper soils for a depth of about 20 cm were treated with the agricultural materials and $^{99}Tc$ 15 d before transplanting. The effects were compared using the transfer factor (TF) defined as the ratio of the plant concentration to the soil concentration. In the case of control plants, TF values for brown rice in the two soils were $4.1{\times}10^{-4}$ and $4.3{\times}10^{-4}$. Of various types of the application, only the application of slaked lime at a lower dose (about 0.6 kg $m^{-2}$), which led to a 60% reduction in the TF value for one soil, seemed to be worth using as a countermeasure. Little effect of the same application was found in the other soil so it is important to determine the effect averaged for a number of soils. Organic fertilizer applications at both of two different doses increased the TF value. It is considered necessary to perform experiments for slake lime applications at doses lower than the above.

• Korean Journal of Environmental Agriculture
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• v.35 no.2
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• pp.87-96
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• 2016

BACKGROUND: The objective of this study is to evaluate the soil quality to cultivate crops in urban garden.METHODS AND RESULTS: During the period of six month from March to August 2015, measured eight heavy metals, seven Organic items and Fluorine on seventeen urban sites and thirty-one suburban sites in Gwangju city.METHODS AND RESULTS: The average concentration and range of heavy metal in soil are 0.15 mg/kg(ND-0.6) for Cd, 14.9 mg/kg(1.5-33.3) for Cu, 4.4 mg/kg(0.4-71.8) for As, 0.05 mg/kg(ND-1.366) for Hg, 24.7 mg/kg(13.1-62.7) for Pb, 102.5 mg/kg(49.1-276.4) for Zn and 9.2 mg/kg(ND-90.1) for Ni but Cr⁶⁺ is not detected. The average value and range of soil fertility items are 253.5mg/kg(76.6-1766.0) for fluorine, 6.4(4.8-7.7) for pH, 20.3 g/kg(5.0-44.0) for orangic matters, 562.7 mg/kg (28.0-1672.0) for available phosphate, 0.6 cmol⁺/kg (0.1-2.3) for K, 9.7 cmol⁺/kg (2.7-22.0) for Ca, 3.0 cmol⁺/kg (0.9-7.4) for Mg, 1.0 ds/m(0.2-2.9) for conductivity. The concentration of Hg in Suburban area is 0.005 mg/kg lower than 0.134 mg/kg in urban area. Also, the concentration of As, Cd, Ni and Zn is lower than urban area as 32%, 37%, 51%, 71% respectively.CONCLUSION: According to SPI index of soil contamination, 39 sites are first degree and 8 sites are second degree and 1 site(41th) is fourth degree. Pb and Cd are not detected and As is detected tiny amounts in plants grown polluted soil, so heavy metals have not moved to plants.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.12 no.4
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• pp.275-286
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• 2014

This study evaluated the evolution stage and origin of chemical components of 12 boreholes at crystalline bedrock using multivariate statistical and groundwater quality analyses. Groundwater types are mostly belonged to Na(Ca)-$HCO_3$ and Ca-$HCO_3$ types, indicating that directly reaction of cation exchange ($Ca^{2+}{\rightarrow}Na^+$) prevailed. The degree of groundwater evolution is included the range from low to intermediate stage based on field and laboratory analytical conditions. As a result of multivariate statistical analysis, a typical indicator of groundwater contamination, $NO_3$-, has the positive correlation with $Na^+$ and $Cl^-$. The origin of sea spary ($Cl^-$) has the positive correlation with $Na^+$, $SO{_4}^{2-}$, $Mg^{2+}$, and $K^+$, while not correlation with $Ca^{2+}$, $Fe^{2+}$, $HCO_3{^-}$, $F^-$, and $SiO_2$. The concentration of $Cl^-$ and $NO_3{^-}$ belongs to general quality of groundwater and not exceeds over the Korean standard for drinking water. And the negative values of saturation index of minerals are calculated with chemical components in groundwater. Therefore, most of chemical components of groundwater in the study area are originated from natural process between rock and groundwater, whereas some of components are derived from sea spary and anthropogenic sources related to agricultural activities.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.11
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• pp.631-635
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• 2015

Water quality investigation was conducted for small/medium sized reservoirs in A city in June and November 2014 as a preliminary step for further use. Water quality analysis experiments were conducted for 21 reservoirs managed by a local government and water quality data were obtained from Korea Rural Community Corporation for 12 reservoirs. It was found that relatively poor water quality, not satisfied with the regulation level (somewhat poor(IV)) for agricultural use, was observed for some reservoirs and worse water quality (poor(V) and very poor(VI) grade) was observed for more reservoirs during autumn /winter season due to the decrease in water volume. Correspondingly, the trophic state was obtained as "Eutrophic" or "Hypertrophic" using the Korean trophic state index for most reservoirs. It was also revealed that significant contamination in sediments was not observed for selected 8 reservoirs considering water quality in each reservoir. The results in this study could be useful for the use of small/medium sized reservoirs as alternative water resources, tourism resources, inland fishery resources, renewable energy production sites, and anti-flooding reservoirs.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1152-1161
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• 2007

An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L. monocytogenes was produced from cloned hybridoma cells (FKLM-3B12-37) and used to develop an ICG strip test. The antibody showed a stronger binding to L. monocytogenes than other Listeria species, and a weak cross-reaction to S. aureus based on an ELISA. The detection limit of the ICG strip test was $10^5\;cell/ml$. In total, 116 meat and processed-meat samples were collected and analyzed using both the ICG strip test and a PCR. The ICG strip test and PCR indicated L. monocytogenes contamination in 34 and 27 meat samples, respectively. The 7 meat samples not identified as L. monocytogenes positive by the PCR were also tested using an API kit and found to be contaminated by Listeria species. In conclusion, the ICG strip test results agreed well with those obtained using the PCR and API kit. Thus, the developed ICG has potential use as a primary screening tool for L. monocytogenes in various foods and agricultural products, generating results within 20 min without complicated steps.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.4
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• pp.424-431
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• 1996

This study was carried out to provide information for establishing counter measures of soil pollution through analysis of Cd. Cu, Pb, and Zn in paddy soils and brown rice. Cadmium, Cu, Pb, and Zn contents in soils were analyzed and distribution maps for these heavy metals were prepared. Heavy metal contents in brown rice were also measured. Average contents of Cd, Cu, Pb, and Zn in surface paddy soils extracted with 0.1 N HCl were 7.4, 35.8, 98.9, and $118.8mg\;kg^{-1}$, respectively. These were 9 times (Cu) to 50 times (Cd) higher than the background level of heavy metals in unpolluted paddy soils in Korea. The contents of Pb and Zn were lower than those measured in 1980, whereas Cd content did not decrease. The levels of heavy metal contamination in paddy soils may not affect growth or yield of rice plant, however, Cd contents indicated a level of serious concern to humans. The average contents of Cd, Cu, Pb, and Zn in brown rice were 0.38, 2.38, 1.31 and $22.31mg\;kg^{-1}$, respectively.