• Molecules and Cells
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• v.28 no.2
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• pp.131-137
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• 2009

Plant defensins are small (5-10 kDa) basic peptides thought to be an important component of the defense pathway against fungal and/or bacterial pathogens. To understand the role of plant defensins in protecting plants against the brown planthopper, a type of insect herbivore, we isolated the Brassica rapa Defensin 1 (BrD1) gene and introduced it into rice (Oryza sativa L.) to produce stable transgenic plants. The BrD1 protein is homologous to other plant defensins and contains both an N-terminal endoplasmic reticulum signal sequence and a defensin domain, which are highly conserved in all plant defensins. Based on a phylogenetic analysis of the defensin domain of various plant defensins, we established that BrD1 belongs to a distinct subgroup of plant defensins. Relative to the wild type, transgenic rices expressing BrD1 exhibit strong resistance to brown planthopper nymphs and female adults. These results suggest that BrD1 exhibits insecticidal activity, and might be useful for developing cereal crop plants resistant to sap-sucking insects, such as the brown planthopper.

• Korean journal of food and cookery science
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• v.26 no.3
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• pp.238-245
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• 2010

Sprout vegetable and general vegetable were obtained from radish, red cabbage, vegetable green, buckwheat and broccoli seeds. The vitamin contents had a large deviation, and the vitamin B1 contents decreased and/or was not detected according to the sprouting. This tendency occurred because of the use for growth of sprouts. The mineral contents in sprout vegetables was the highest and was 2~10 times greater than general vegetables. In the growing pathway, potassium was a large consumption nutrient and consumption of sodium was low. $\alpha$-Amylase and total enzyme activities were higher in sprout vegetable than general vegetable, which was also the case of the corresponding freeze dried powders. Sprouting was shown to increase the enzyme activities. Thus, sprout vegetables had several advantages including a rich level of nutrients and safety from agricultural pesticides.

• The Plant Pathology Journal
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• v.29 no.2
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• pp.125-135
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• 2013

In agro-ecosystems worldwide, some of the most important and devastating diseases are caused by soil-borne necrotrophic fungal pathogens, against which crop plants generally lack genetic resistance. However, plants have evolved approaches to protect themselves against pathogens by stimulating and supporting specific groups of beneficial microorganisms that have the ability to protect either by direct inhibition of the pathogen or by inducing resistance mechanisms in the plant. One of the best examples of protection of plant roots by antagonistic microbes occurs in soils that are suppressive to take-all disease of wheat. Take-all, caused by Gaeumannomyces graminis var. tritici, is the most economically important root disease of wheat worldwide. Take-all decline (TAD) is the spontaneous decline in incidence and severity of disease after a severe outbreak of take-all during continuous wheat or barley monoculture. TAD occurs worldwide, and in the United States and The Netherlands it results from a build-up of populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. during wheat monoculture. The antibiotic 2,4-DAPG has a broad spectrum of activity and is especially active against the take-all pathogen. Based on genotype analysis by repetitive sequence-based-PCR analysis and restriction fragment length polymorphism of phlD, a key 2,4-DAPG biosynthesis gene, at least 22 genotypes of 2,4-DAPG producing fluorescent Pseudomonas spp. have been described worldwide. In this review, we provide an overview of G. graminis var. tritici, the take-all disease, Pseudomonas biocontrol agents, and mechanism of disease suppression.

• Biomolecules & Therapeutics
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• v.16 no.4
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• pp.385-393
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• 2008

As an attempt to search for bioactive natural products exerting anti-inflammatory activity, we evaluated the effects of the methanol extract of Polytrichum commune Hedw (PCM) (Polytrichaceae) on lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$) and pro-inflammatory cytokines release in murine macrophage cell line RAW 264.7. PCM potently inhibits the production of NO, $PGE_2$, tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6. Consistent with these results, PCM also concentration-dependently inhibited LPS-induced inducible NO synthase (iNOS) and cyclooxygase (COX)-2 at the protein levels, and iNOS, COX-2, TNF-$\alpha$ and IL-6 at the mRNA levels without an appreciable cytotoxic effect on RAW 264.7 macrophag cells. Furthermore, PCM inhibited LPS-induced nuclear factor-kappa B (NF-$\kappa$B) activation as determined by NF-$\kappa$B reporter gene assay, and this inhibition was associated with a decrease in the nuclear translocation of p65 and p50 NF-$\kappa$B. Taken together, these results suggest that PCM may play an anti-inflammatory role in LPS-stimulated RAW 264.7 macrophages through the inhibitory regulation of iNOS, COX-2, TNF-$\alpha$ and IL-6 via NF-$\kappa$B inactivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.3
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• pp.320-324
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• 2007

To characterize flooding-affected anatomical morphology changes in soybean, flooding-tolerant cv Pung-sannamulkong (PSNK) and flooding-sensitive cv. Sobaek-namulkong (SBNK) at 5 trifoliate leaf stage were exposed to flooding by maintaining tap water 2 cm above soil surface for 9 days and resultant microscopic anatomical changes in leaf and hypocotyl cross-sections along with chlorophyll content and photosynthetic rate changes were compared. Flooding-sensitive SBNK exhibited more significant decrement in chlorophyll content, photosynthetic rate, number of palisade parenchyma, and leaf dry matter compared to cv. PSNK. Flooding decreased fineness of palisade parenchyma, while inducing wider xylem vessel, especially in PSNK. The aerenchyma formation in hypocotyls under flooding could be observed only in flooding-resistant PSNK. All these anatomical changes seems to be related with higher physiolocial activity and resultant resistance against flooding in PSNK compared to flood-sensitive PSNK.

• Korean Journal of Environmental Agriculture
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• v.2 no.2
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• pp.103-107
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• 1983

The effect of photochemical oxidants on rice plants was measured by growing the rice plants Nihonbare in pot in charcoal-manganese oxide filtered atmosphere and non-filtered air. Visible injury on the leaf blades of rice plants were observed in plants grown under the unfiltered air chamber, but plants under filtered air chamber were free from any injury. Fresh weight of stem and root at maximum tillering stage in unfiltered chamber were 16.8 and 46.4% less than filtered air chamber, respectively. Grain yield in unfiltered air chamber was also reduced by 14.7% compared to that of filtered air chamber. And the reduced yield paralleled increase in concentration of oxidants in the atmosphere at the experimental site. ABA content in rice plants cultivated in unfiltered air chamber was higher than in filtered air chamber, but the root activity of rice plants in unfiltered air chamber was remarkably decreased.

• Korean Journal of Environmental Agriculture
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• v.20 no.1
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• pp.57-62
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• 2001

Twelve antagonistic strains against Fusarium wilt of watermelon-rootstock gourd were selected from 54 bacterial isolates which were isolated from the rhizosphere of crop plants growing in various locations. They showed strong inhibitory effects on growth of Fusarium osysporum f. sp. niveum, the causal agent of watermelon-rootstock gourd Fusarium wilt. Among these antagonists, the isolate YJ-3 was the most pronounced in growth-promoting ability for watermelon-rootstock gourd. The growth of watermelon-rootstock gourd in bed soil inoculated with YJ-3 was better by 46 and 13% than those in commercial bed soil alone and in bed soil inoculated with commercial microbial inoculant, respectively. The antagonistic plant growth-promoting rhizobacterium, strain No. YJ-3, was identified as Bacillus sp. on MIDI system. Furthermore, Bacillus sp. YJ-3 showed antifungal activity on growth against Alternaria cucumerina, Botrytis cinerea, Colletotrichum orbiculare, Didymella bryoniae, Rhizoctonia solani and Fusarium oxysporum.

• Journal of Engineering Education Research
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• v.16 no.1
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• pp.27-34
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• 2013

The purpose of this study is to analyze work values of undergraduates according to major and gender. The result of this research is following. First, undergraduates from engineering and social science department took a very serious view of achievement and leasure of body and mind in work values. Second, undergraduates made much of achievement, job stability, leasure of body and mind, economic compensation and reputation. And undergraduates thought that internal value orientation was more important than external value orientation. Third, two groups had significant difference in service, knowledge pursuit, patriotism, economic compensation, reputation, and indoor activity. Fourth, the result of this analysis according to gender is following. Male undergraduates made much of leasure of body and mind, achievement, job stability, knowledge pursuit, and economic compensation in order. But female undergrduates made much of economic compesation, leasure of body and mind, achievement, and job stability in order. Fifth, that undergraduates make little of domain of service and patriotism gives a suggestion as to improvement of course education. As a result, this study is suggestive of instruction and advice in course search curriculum.

• Korean Journal of Community Nutrition
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• v.17 no.6
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• pp.772-781
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• 2012

In order to supply the reference data to define the quality of life of the rural agricultural elderly population in the simplified steps, the data were collected by personal visits to 232 elder people over 65 (89 males and 143 females) in Sunchang area. The survey was conducted with written questionnaires concerning the quality of life, health-related habits, basic physical functions and cognitive behavior. The prevalence of the degenerative diseases were assessed by anthropometric and biochemical determinations. The averaged overall quality of life represented by EQ-5D index with Nam's model was calculated to be $0.865{\pm}0.1509$, and the percentages of people below the average were 38% (low QOL group) and 62% (high QOL group) were found to be the above the average. Generally, the subjects with male gender, higher educational background, higher self-rated health status, higher social activities were belonged to the high QOL group. The high QOL group had higher scores of daily living activities KADL and IADL, and lower risks in cognitive functions K-MMSE and depression scale GDS. There was no statistically significant correlation between biochemical indexes of blood and cognitive function and EQ-5D scores when the results were adjusted for age and gender. There were significant differences in nutritional assessment determined by the MNA between the high and low QOL groups. The low QOL group showed inferior nutritional status. The rapidly measurable factors of the quality of life of rural elderly were turned out to be physical activity scores and the simplified nutritional status measurement.

• Korean Journal of Environmental Agriculture
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• v.3 no.1
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• pp.71-78
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• 1984

The light quality obtained from sunlight by removing the spectral region below about 500 nm(blue light region) showed a remarkable effect on the growth of pepper. The physiological characteristics evaluated with the conventionally adopted indices at the vegetative stage revealed that the filtered sunlight improved and accelerated growth in general. In addition, the photosynthetic pigment level comprising chlorophylls, ${\beta}-carotene$ and 5 xanthophyll components as major pigments was increased significantly in the leaves grown under the orange-colored light. The increase of pigment content was prominent for every carotenoid component rather than for chlorophylls. The strong growth activity produced by the elected spectral quality for this work was properly demonstrated by the fruit yield ratio (filtered light/white light) which was found to be 1.35. Also confirmed was that removing the blue light region from solar radiation did not result in worsening of the food quality of matured pepper. The logic behind selection of the above light quality and some data presented was discussed on various photobiological aspects.