• Animal cells and systems
• /
• 제2권1호
• /
• pp.107-111
• /
• 1998

Two kinds of humoral factors were observed in 2 orders, 7 families, 10 genera, and 15 different species of Korean ascidians. They are the naturally occuring hemagglutinins and/or hemolysins against human erythrocytes A, B, and 0. All but two species showed aggregative activity, although there were considerable variations in titer. The weak agglutinating and lytic activities were increased in the presence of $Ca^{++}$. Much higher activities of agglutination and/or lysis were shown in the hemolymph than extracts from tissues, and a higher response was shown in adults than in juveniles. No distinct differences from collected locations were observed. The hemolymph of Ciona intestinalis showed a strong hemolytic (cytotoxic) and weak agglutinins capacities. In addition, hemolymph of Styela plicata and Styela clava clava also showed hemoagglutining and hemolytic activities. Botryllus tuberatus had hemagglutining and weak lytic activities. Other species showed only hemagglutining activity. These agglutining activities are probably responsible for carbohydrate recognition in solitary or colonial ascidian. The lytic activity is probably responsible for antibacterial defense and nonfusion reactions between allogeneic colonial ascidians, especially the genus of Botryllus. The occurrences of humoral factors in ascidians were independent of their geographic distributions.

• 대한수의학회지
• /
• 제12권1호
• /
• pp.91-95
• /
• 1972

Investigation of leptospiral antibody in Korean cattle and pigs was carried out from February to October, 1971. Ten different living antigens, namely L. icterohaemorrhagiae, L. canicola, L. antumnalis, L. hebdomadis, L. australis A, L. pomona, L. pyrogenes, L. grippotyphosa, L. bataviae and L. javanica, were used. A total of 590 Korean cattle and 460 pig blood samples collected from Seoul Majang-dong slaughterhouse were tested by the rapid microscopic agglutination test. Throughout the studies the following results were obtained and summarized. 1. Of 590 serum samples of Korean cattle 51 were positive(8.64%). 2. Of 460 serum samples of pigs 27 were positive (5.87%). 3. Of 51 positive cattle samples, 29(4.92%) showed antibody to a serotype of L. icterohaemorrhagiae and 18(3.0%) to L. canicola, and 4 (0.68%) to L. pomona. Eight of L. icterohaemorrhagiae positive samples showed a cross reaction to L. canicola. 4. Of 27 positive pig samples, 14(3.04%) showed antibody to L. ictereohaemorrhagiae and 7(1.52%) to L. grippotyphosa. 4(0.87%) to L. canicola, 2(0.43%) to L. pomona. Two of L. canicola positive samples showed a cross reaction to L. grippotyphosa. 5. Serum samples of seven pigs, showing antibody positive to L. grippotyphosa were first observed in Korea. 6. Infection rate of bovine and porcine leptospirosis, in Korea, appeared to be lower than that of Japan, Taiwan, Thailand and the Philippines.

• Asian-Australasian Journal of Animal Sciences
• /
• 제10권2호
• /
• pp.245-250
• /
• 1997

Highly significant correlation (p < 0.001) was found between body weight and chest circumference of the local Katjang and cross-bred (local Katjang ♀ ${\times}$ German Fawn ♂ bucks under study. Increase in body length, chest circumference, depth of chest and height at withers (p < 0.001) reflected significantly the increase in body weight of the bucks. At the same age the cross-breds were bigger than the indigenous breed. No significant correlation was detected between body weight and scrotal circumference, or the latter with sperm counts in both buck types under study. However, the fluid portion of the semen increased in acidity and volume, the latter being significant (P < 0.01) in the local Katjangs, with increase in scrotal circumference. Although the effects of body condition on buck libido of both groups were not significant, the reaction time taken to mount the teaser females were significantly diminished (p < 0.001) with better body condition, at least in the local Katjangs. The reaction time gad an inverse, though not necessarily significant, relationship with semen characteristics such as volume, pH (in local Katjangs, p < 0.05), concentration, color (in cross-breds, p < 0.05), agglutination and mass movements in both phenotypes.

• 대한미생물학회지
• /
• 제34권6호
• /
• pp.583-589
• /
• 1999

Acellular pertussis vaccine has been used widely in Korea since 1984. However, because many of the former generations were not inoculated with pertussis vaccine, they may infect infants with pertussis. With this background, we investigated the prevalence of pertussis antibodies in all age groups. Enzyme-linked immunosorbent assay (ELISA) to assess IgG antibodies to pertussis toxin (PT) and filamentous hemagglutinin (FHA) and bacterial agglutination (BA) to assess antibodies to agglutinogen were compared on 842 serum samples which were donated from 11 hospitals in Seoul area. In comparison with age groups under 20 years, antibodies of adults against PT and FHA were maintained. But antibodies against agglutinogen showed no pattern in all age groups. Antibodies to PT were correlated with antibodies to FHA. There was no significant difference in antibody levels between male and female (p<0.05).

• 한국어병학회지
• /
• 제24권3호
• /
• pp.189-195
• /
• 2011

Starry flounder, which are recently increasingly cultured in Korea, are known to highly vulnerable to Streptococcus parauberis infection. Five groups of starry flounder (n=30 for each group) were vaccinated with S. parauberis formalin-killed whole cells by intraperitoneal injection at a final concentration of 0, 0.01, 0.1, 1 and 10 mg $fish^{-1}$. Specific antibody production of 1 and 10 mg $fish^{-1}$ administered groups significantly increased at four weeks post immunization. All vaccinated groups showed higher survival rates than a control group when five groups of fish were challenged with S. parauberis at a dose of $1.14{\times}10^4$ cfu $fish^{-1}$ and $1.14{\times}10^2$ cfu $fish^{-1}$, respectively. In particular, 0.1 or higher concentrations of formalin killed bacterial cells are able to confer the fish high protection against S. parauberis infection.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제6권1호
• /
• pp.27-31
• /
• 2003

Infectious flacherie of silkworm Bombyx mori is caused by B. mori infectious flacherie virus (BmIFV) and causes severe crop loss to sericulturists. In the present study, a colloidal textile dye-based dipstick immunoassay is developed for the detection of infectious flacherie in silkworms. Colloidal textile dye (blue D2R) with Aλ$_{max}$ at 620 nm was sensitised with 500 $\mu\textrm{g}$/ml of purified anti-BmIFV IgG. The dye-antibody reagent detects purified antigen up to 10 ng/ml and BmIFV infection in diseased larval extracts $(up to a dilution of {10^-5})$ and faecal matter extracts $(up to a dilution of {10^-2})$ by forming clear blue dot within 30 min. It was observed to be stable for three months period at $4^{\circ}C$. The efficacy of textile dye-based dipstick immunoassay was on pay with HRP-based dipstick immunoassay and fluorescent antibody test, and better than latex agglutination and ouchterlony tests in the detection of BmIFV The dye-based dipstick immunoassay method provides a simple, sensitive and less expensive test for the detection of BmIFV infection in silkworms.s.

• 한국연초학회지
• /
• 제1권1호
• /
• pp.33-38
• /
• 1979

低濃度의 바이러스 조건하에서도 반응의 민감도가 높은 바이러스病의 血淸學的 診斷法을 개발하고자 담배모자이크 바이러스(Tobacco mosaic virus), 보리줄무늬 모자이크 바이러스( Barleys-tripe mosaic virus) 및 大豆모자이크 바이러스(Soybean mosaic virus)의 抗血淸으로부터 電氣泳動에 의해 추출된 Immunoglobulin을 Latex(0.8l$\mu$, Difco)粒子에 흡착시켜 梢子毛細管(1 $\times$ 100mm)내에서 各 罹病組織의 汁液과 반응시킨 결과 寒天沈降法이나 微量沈降法보다 민감도가 매우 높았다. 이 방법은 작업과정이 간편하고 빠른 시간내에 다량의 시료를 檢定할 수 있으며 陽性反應과 陰性反應을 쉽게 구별할 수 있었다.

• 한국임상수의학회지
• /
• 제17권1호
• /
• pp.145-151
• /
• 2000

황색포도상구균이 Capsular polysaccharide (CPS)는 백혈구의 탐식에 저항하기 때문에 젖소의 유방염 발생의 중요한 병원상 인자로 오랫동안 연구자들의 관심이 집중되어 왔다. 따라서 CPS는 젖소의 유방염을 최소화시키기 위한 유방염 백신개발에 있어 잠재적인 백신 항원물질로 알려져 왔다. 본 연구의 복적은 황색포도상구균의 CPS 항원물질을 좀더 효과적으로 발현시키고 향후 백신항원으로 사용시 좀 더 간편하게 정제하는 방법을 찾아보고자 본 연구를 수행하였다. CPS는 락토스와 덱스트로스를 첨가한 brain heart infusion media 에서도 부분적으로 발현되었지만 CPS가 가장 잘 발현되는 배지 조건은 젖소 유래의 유청을 10% 첨가한 조건에서 가장 발현율이 높았다. 또한 CPS발현은 salt agglutination test, india ink법 및 투과전자현미경을 이용하여 확인하였으며 균체표면의 hydrophobicity에서는 락토스, 덱스트로스 및 젖소 유래의 유청을 첨가한 배지조건에서 다른 조건에 비해 높았다. CPS의 가장 손쉬운 검사법은 균체를 절편하지 않고 직접 투과전자현미경상에서 염색없이 관찰하는 것이 가장 간편한 방법이었다. CPS정제는 상층액을 농축시킨후 이온교환크로마토그라피와 젤 여과법을 이용하여 정제하였으며 CPS의 분자량은 대부분 97kDa이상이었다. CPS 회수율은 배지와 균체 1리터당 1.5mg이었다. 결론적으로 CPS를 백신항원으로 포함하고자 할 경우, CPS의 최적 발현조건은 젖소 유래의 유청을 10% 첨가한 Brain heart infusion 배지에서 배양하여 사용하는 것이 가장 좋은 방법이라고 생각된다.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• 제1권1호
• /
• pp.138-143
• /
• 1998

저자들은 만성 설사 및 점액성 혈변을 주소로 내원한 25개월 남아에서 대장 내시경 및 조직생검, latex 응집 반응검사로 위막성 대장염 1례를 진단, 치료하였기에 보고하는 바이다.

• 대한한의학원전학회지
• /
• 제21권3호
• /
• pp.147-155
• /
• 2008

Seopcheonsa(葉天士) regarded the cause of Stomach-Eum[胃陰] deficiency as the lack of fluid and humor induced the heat by Eum(陰, yin) deficiency constitution, external pathogen, overexertion and fatigue, misused medication etc. He said that the symptoms of Stomach-Eum deficiency was related to indigestion of food and that it induced the short supply of nutrition for the whole body and the symptoms by disabled Earth[土]-engenders-Metal[金]. He presented the treatment of clearing Stomach-Fire[胃熱] and nourishing Stomach-Eum[胃陰], and used Geumgwemaekmundongtang(金匱麥門冬湯). And he emphasized Stomach-Eum to supplement existing Spleen-Yang[脾陽] centered treatment. Dangjonghae(唐宗海) regarded Spleen-Eum[脾陰] as these, first, it was fluid and humor such as digestive enzyme. Secondly, it was nutritive substance itself. Finally, it contained plaster-oil and blood-agglutination which formed flesh with nutritive substance. Seopcheonsa and Dangjonghae both thought that fluid and humor was necessary to digest food. However, Dangjonghae's Spleen-Eum[脾陰] included nutritive substance as the result of digestion. So we can say that Dangjonghae integrated Spleen-Eum with Stomach-Eum and Spleen-nutrient.