• Journal of Microbiology and Biotechnology
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• 제30권3호
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• pp.325-332
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• 2020

Methyl linderone (ML), a cyclo-pentenedione, was isolated from the fruit of Lindera erythrocarpa Makino (family Lauraceae). This plant has well-known anti-inflammatory effects; however, the anti-cancer effects of ML have not yet been reported. Thus, in the present study we investigated the effects of ML on the metastasis of human breast cancer cells. We used 12-O-tetradecanoyl phorbol-13-acetate (TPA)-stimulated MCF-7 cells as the cell model to study the effects of ML on invasion and migration. ML was found to reduce the invasion and migration rate of TPA-stimulated MCF-7 cells. Moreover, it inhibited two metastasis-related factors, matrix metalloproteinase-9 (MMP-9) and interleukin-8 (IL-8), at the mRNA and protein expression levels, in TPA-treated MCF-7 cells. The mechanism by which ML exerted these effects was through the inhibition of translocation of activator protein-1 (AP-1) and signal transducer and activator of transcription-3 (STAT3), mediated via phosphorylation of extracellular signal-regulated kinase (ERK). Taken together, our findings indicated that ML attenuated the TPA-stimulated invasion and migration of MCF-7 cells by suppressing the phosphorylation of ERK and its downstream factors, AP-1 and STAT3. Therefore, ML is a potential agent for the treatment of breast cancer metastasis.

• 한국콘텐츠학회논문지
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• 제9권12호
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• pp.932-939
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• 2009

본 논문은 시맨틱 웹 서비스 환경에서 유아교육을 위한 각각의 서비스를 표현하는 메타 정보를 기술한 서비스 템플릿 객체 모델을 기반으로 그래픽한 서비스 템플릿 저작도구 시스템을 구현하였다. 제안한 시스템은 자동으로 웹 서비스를 선택하고 구성하여 적절한 서비스 플랜을 생성하여 로봇 서비스를 제공한다. 또한 STDL을 기반으로 URC용 서비스 템플릿 생성, 추가, 삭제, 수정 등의 기능뿐만 아니라 서비스 템플릿 리소스들에 대한 그래픽한 처리 기능을 제공한다. 서비스 템플릿 과정에서 사용자 편의적인 환경 제공을 위해 플로우 뷰 스타일, 그리드 뷰 스타일, 텍스트 뷰 스타일 등의 편집 환경을 구현했다. 또한 실제 로봇 구동에 필요한 로봇API를 참조하고 이를 기반으로 추상 서비스 블록을 구체화하여 서비스 템플릿을 쉽게 작성할 수 있는 기능을 제공하게 된다. 따라서 서비스의 시맨틱 정보를 기반으로 서비스 에이전트의 지능적이고 자율적인 서비스를 제공할 수 있다.

• 정보처리학회논문지B
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• 제17B권1호
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• pp.85-92
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• 2010

부분 의미 분석 시스템은 문장의 구성 요소들이 술어와 갖는 관계를 분석하는 것으로 문장에서 술어의 주체, 객체, 도구 등을 나타내는 의미 논항을 확인하게 된다. 본 논문에서 개발한 부분 의미 분석 시스템은 두 단계로 구성되어 있는데, 먼저 부분 구문 분석 결과로부터 의미 논항의 경계를 찾는 의미 논항 확인 단계를 수행하고 다음으로 확인된 의미 논항에 적절한 의미역을 부착하는 의미역 할당 단계를 수행한다. 순차적인 두 단계 방법을 적용하는 것에 의해서, 학습 성능 저하의 주요한 원인인 클래스 분포의 불균형 문제를 완화할 수 있고, 각 단계에 적합한 자질을 선별하여 사용할 수 있다. 본 논문에서는 PropBank 말뭉치에 기반한 CoNLL-2004 shared task의 데이터 집합 및 평가 프로그램을 사용하여 각 단계가 시스템의 전체 성능에 기여하는 정도를 보인다.

• 약학회지
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• 제36권6호
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• pp.518-528
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• 1992

To determine the optimal conformation of sulfonylureas, the correlation between conformation and hypoglycemic activity of the two sulfonylureas of tolbutamide and chlorpropamide as hypoglycemic agent was studied using an empirical potential function (ECEPP/2) and the hydration shell model in the unhydrated and hydrated states. The conformational energy was minimized from several starting conformations with possible torsion angles in each molecule. The conformational entropy change of each conformation was computed using a harmonic approximation. To understand the hydration effect on the conformation of the molecules in aqueous solution, the contribution of water-accessible volume of each group or atom in the lowest-free-energy conformation was calculated and compared each other. From comparison of the computed lowest-free-energy conformations of two sulfonylureas, it could be suggested that the hydration of sulfonylurea moiety is related to increase the hypoglycemic activity. From the calculation results, it was known that the conformational entropy is the major contribution to stabilize the low-free-energy conformations of two sulfonylureas in unhydrated state. Whereas, in hydrated state, the hydration free energy largely contributes to the total free energies of low-free-energy conformations of tolbutamide and conformational entropy contributes to stabilize the low-free-energy conformations of chlorpropamide. The torsion angles from phenyl ring to urea moiety of the low-free-energy conformations of the two sulfonylureas were shown the nearly regular trend. On the basis of these results, the conformation exhibiting the optimal hypoglycemic activity of sulfonylureas and the binding direction to pancreatic receptor site A could be predicted. Also, according to the side chain lengthening of urea moiety, tolbutamide showed various conformational change. Therefore, steric effect may be important factor in the interaction between sulfonylureas and the putative pancreatic receptor.

• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.245-253
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• 2012

Collagen tripeptide (CTP) is a functional food material with several biological effects such as improving dry skin and wound and bone fracture healing. This study focused on the anti-photoaging effects of CTP on a hairless mouse model. To evaluate the effects of CTP on UVB-induced skin wrinkle formation in vivo, the hairless mice were exposed to UVB radiation with oral administration of CTP for 14 weeks. Compared with the untreated UVB control group, mice treated with CTP showed significantly reduced wrinkle formation, skin thickening, and transepidermal water loss (TEWL). Skin hydration and hydroxyproline were increased in the CTP-treated group. Moreover, oral administration of CTP prevented UVB-induced MMP-3 and -13 activities as well as MMP-2 and -9 expressions. Oral administration of CTP increased skin elasticity and decreased abnormal elastic fiber formation. Erythema was also decreased in the CTP-treated group. Taken together, these results strongly suggest that CTP has potential as an anti-photoaging agent.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제5권7호
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• pp.1329-1345
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• 2011

After the 9.11 terror attack, lawful Interception (LI) has emerged as an important tool for anti-terrorist activity. Law enforcement agents and administrative government bodies effectively monitor suspicious target users of permanent IP-based network devices by LI in Packet Data Networks (PDNs). However, it is difficult to perform LI in monitoring migrating users from a location to another, who change their IPs due to the proliferation of portable Internet devices enabling 3G IP Multimedia Subsystems (IMS). The existing, manual handover technique in 3G IMS makes it even more difficult to continue the LI activities due to time-lag reissuance of LI authority warrants when the target users move to a new LI jurisdiction via a roaming service. Our proposed model is a seamless LI handover mechanism in 3G IMS to support mobility detection of the target users. The LI warrants are transferred to the new LI agent automatically with the target users when they move to a new LI jurisdiction. Thus, time-lag human intervention of reissuance of the LI warrants is removed and enables the LI authorities to continue monitoring. In the simulation of our proposed mechanism, the quality of lawful interception achieves a mean score of over 97.5% out of the possible 100% maximum score, whereas the quality of the existing mechanism has a mean score of 22.725%.

• 한국화재소방학회논문지
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• 제29권4호
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• pp.26-32
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• 2015

이산화탄소 소화설비는 질식소화가 주된 소화작용이므로 제한된 시간 내에 소화약제를 방출하여 방호구역의 산소농도를 기준치 이하로 유지시키는 능력이 확보되어야 한다. 국내에서 이산화탄소 소화설비는 국민안전처 고시에 따라 인증된 설계프로그램을 사용하여 소화설비를 설계하고 있다. 그러나 소화설비가 설치된 방호구역의 기하학적 구조는 성능인증 단계의 모의실험장치와 차이가 있기 때문에 설계프로그램 적용 범위를 벗어난 소화설비 설계가 이루어질 수 있다. 특히, 저압식 이산화탄소 소화설비는 배관 내 유동현상에 따라 소화약제 방출량이 변하므로 소화설비 설치 후에 현장 방출시험을 통하여 성능을 확인할 필요가 있다. 현재까지 국내 외 기술기준에는 방출시험에 대한 방법론이 구체적으로 명시되어 있지 않다. 본 논문에서는 국내외 기술기준을 비교 분석하고, 방출시험 사례분석을 통하여 현장 방출시험 방법론을 제시하였다.

• Toxicological Research
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• 제32권2호
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• pp.133-140
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• 2016

Sulfasalzine is a widely administered drug against inflammatory-based disorders in human. However several cases of liver injury are associated with its administration. There is no stabilized safe protective agent against sulfasalazine-induced liver injury. Current investigation was designed to evaluate if N-acetylcysteine (NAC) and dithioteritol (DTT) as thiol reducing agents and/or vitamins C and E as antioxidants have any protective effects against sulfasalazine-induced hepatic injury in an ex vivo model of isolated rat liver. Rat liver was canulated and perfused via portal vein in a closed recirculating system. Different concentrations of sulfasalazine and/or thiol reductants and antioxidants were administered and markers of organ injury were monitored at different time intervals. It was found that 5 mM of sulfasalazine caused marked liver injury as judged by rise in liver perfusate level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) (p < 0.05). A significant amount of lipid peroxidation and hepatic glutathione depletion were detected in drug-treated livers, accompanied with significant histopathological changes of the organ. Administration of NAC ($500{\mu}M$), DTT (${400\mu}M$), Vitamin C ($200{\mu}M$), or vitamin E ($200{\mu}M$) significantly alleviated sulfasalazine-induced hepatic injury in isolated perfused rat liver. The data obtained from current investigation indicate potential therapeutic properties of thiol reductants and antioxidants against sulfasalazine-induced liver injury.

• 기술혁신연구
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• 제19권1호
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• pp.177-201
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• 2011

본 연구는 특허정보활용과 연구개발활동 사이의 관계에 대한 분석을 위해, 국가연구개발사업을 대상으로 특허기술동향조사가 국가연구개발사업의 성과에 미치는 영향을 기술적 성과인 특허성과에 집중하여 그 영향을 추정하였다. 정부는 2005년부터 국가연구개발사업에 대하여 특허기술동향조사사업을 시행하고 그 범위를 확대해 왔다. 특허기술동향조사사업은 국가연구개발사업의 기획단계 또는 과제 선정단계에서 특허 동향 및 선행기술에 대한 정보를 제공하여 중복 투자, 유사기술 개발의 가능성을 줄여 국가연구개발사업의 효율성과 성과를 제고하기 위한 사업이다. 이러한 특허기술동향조사가 국가연구개발사업의 기술적 성과에 어떠한 효과를 지니는지를 투입요소, 개발주체의 특성, 자금의 공급자 요인을 고려한 모형을 사용하여 추정하였다. 2005년부터 2008년까지 국가연구개발사업의 성과 자료와 특허기술동향조사사업에 대한 자료를 바탕으로 특허기술동향조사 사업의 효과를 추정한 결과, 선행기술조사는 국내 및 해외 특허의 출원 및 등록 성과를 유의하게 제고하는 것으로 밝혀졌다. 이러한 결과로부터 국가연구개발사업에서 특허정보활용을 통해 기술적 성과의 제고가 가능하다는 점을 알 수 있다. 이는 특허정보활용을 통해 국가연구개발사업의 효율성과 효과성이 제고되었음을 보여준다.

• 대한본초학회지
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• 제25권3호
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• pp.117-121
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• 2010

Objective：The Herbal Remedy for Diabetes Mellitus-01 (HRDM-01) is composed of 11 species of medicinal plants. HRDM-01 is used as anti-hyperglycaemic agent. Anti-hyperglycaemic agents in western medicine often have hepatotoxicities. Therefore, we investigated safety of HRDM-01, especially in liver functions. Methods：We investigated the effects of HRDM-01 on liver function measuring serum AST, ALT and LDH levels and histopathological changes of liver tissue using photomicroscope. In addition, we also investigated the effects on serum lipid levels such as total cholesterol, HDL-cholesterol and triglyceride. Results：In our experiment, single injection of streptozotocin elevated levels of AST and ALT in serum. But LDH level was not affected. In addition, our animal model showed elevated levels of total cholesterol and triglyceride in serum. 30 days treatment with HRDM-01 lowered serum AST level. Serum levels of ALT and LDH did not affected. In addition, HRDM-01 lowered serum triglyceride level significantly. In histopathological observation, we did not find any abnormal changes in all experimental groups. Conclusions：Briefly, HRDM-01 did not elevate serum levels of ALT, LDH, total cholesterol and did not affect histopathological changes in liver tissues. Moreover, HRDM-01 lowered serum AST, triglyceride, which are elevated by induction of diabetes mellitus. These results suggest the safety of HRDM-01 in diabetic treatment.