• IMMUNE NETWORK
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• 제15권2호
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• pp.91-99
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• 2015

Herpes simplex virus (HSV) is a common causative agent of genital ulceration and can lead to subsequent neurological disease in some cases. Here, using a genital infection model, we tested the efficacy of vinegar-processed flos of Daphne genkwa (vp-genkwa) to modulate vaginal inflammation caused by HSV-1 infection. Our data revealed that treatment with optimal doses of vp-genkwa after, but not before, HSV-1 infection provided enhanced resistance against HSV-1 infection, as corroborated by reduced mortality and clinical signs. Consistent with these results, treatment with vp-genkwa after HSV-1 infection reduced viral replication in the vaginal tract. Furthermore, somewhat intriguingly, treatment of vp-genkwa after HSV-1 infection increased the frequency and absolute number of $CD3^-NK1.1^+NKp46^+$ natural killer (NK) cells producing interferon (IFN)-${\gamma}$ and granyzme B, which indicates that vp-genkwa treatment induces the activation of NK cells. Supportively, secreted IFN-${\gamma}$ was detected at an increased level in vaginal lavages of mice treated with vp-genkwa after HSV-1 infection. These results indicate that enhanced resistance to HSV-1 infection by treatment with vp-genkwa is associated with NK cell activation. Therefore, our data provide a valuable insight into the use of vp-genkwa to control clinical severity in HSV infection through NK cell activation.

• 동아시아식생활학회지
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• 제25권5호
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• pp.813-821
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• 2015

Asthma is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. The purpose of the present study was to evaluate the anti-inflammatory and anti-asthma effects of Adenophora triphylla var. japonica extracts. We investigated the molecular mechanism underlying the effects of 80% ethanol extracts (AE) of A. triphylla on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. AE treatment inhibited pro-inflammatory cytokines such as TNF-${\alpha}$ and IL-6 as well as nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. In particular, NO and pro-inflammatory cytokine production was suppressed more effectively by aerial parts (AE-A) than roots (AE-R) of A. triphylla. Quantitative RT-PCR assay showed that AE reduced mRNA levels of iNOS and COX-2. We also evaluated the anti-asthmatic effects of AE-A in an ovalbumin (OVA)-induced BALB/c mouse model. AE-A supplementation significantly reduced the amounts of airway eosinophils, IL-4 and IL-13 levels in BALF, and IgE levels in serum as compared with untreated, OVA-induced mice. These results suggest that AE-A can be considered as a therapeutic agent to potentially relieve asthma.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3909-3919
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• 2013

The aim of the present investigation was to evaluate the effect of A ferruginea extract on Dalton's lymphoma ascites (DLA) induced tumours in BALB/c mice. Experimental animals received A ferruginea extract (10 mg/kg.b.wt) intraperitoneally for 14 consecutive days after DLA tumor challenge. Treatment with extract significantly increased the life span, total white blood cell (WBC) count and haemoglobin (Hb) content and decreased the level of serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (${\gamma}$-GT) and nitric oxide (NO) in DLA bearing ascites tumor models. In addition, administration of extract significantly decreased the tumour volume and body weight in a DLA bearing solid tumor model. The levels of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin-1 beta (IL-$1{\beta}$), interleukin-6 (IL-6) and granulocyte monocyte-colony stimulating factor (GM-CSF), as well as pro-angiogenic growth factors such as vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) were elevated in solid tumour controls, but significantly reduced by A ferruginea administration. On the other hand, the extract stimulated the production of interleukin-2 (IL-2) and interferon-gamma (IFN-${\gamma}$) in animals with DLA induced solid tumours. Increase in $CD4^+$ T-cell population suggested strong immunostimulant activity for this extract. GC/MS and LC/MS analysis showed quinone, quinoline, imidazolidine, pyrrolidine, cyclopentenone, thiazole, pyrazole, catechin and coumarin derivatives as major compounds present in the A ferruginea methanolic extract. Thus, the outcome of the present study suggests that A ferruginea extract has immunomodulatory and tumor inhibitory activities and has the potential to be developed as a natural anticancer agent.

• 한국재난정보학회 논문집
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• 제13권1호
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• pp.43-50
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• 2017

본 연구에서는 연약지반이나 부분침하의 복원에 사용되는 팽창약액의 지반내 강도증진 및 응력전달효과를 알아보기 위하여 팽창성능을 구분한 팽창약액을 제작하여 모래지반내의 밀도 및 토압을 측정하여 분석하였다. 상대적 고팽창군과 저팽창군으로 구분하여 별도의 불투수 진공시트에 주입하여 팽창보강제를 제작하고 이를 균질하게 조성된 모형지반하부에서 고결실험을 시행하였다. 이 결과 팽창보강제 상부 약 15cm까지 보강효과가 나타났고, 토압의 분석결과 $1.150{\sim}11.298t/m^2$의 집중하중과 유사한 다짐경향이 나타났다.

• 한국축산식품학회지
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• 제37권5호
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• pp.646-653
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• 2017

Alcalase hydrolysis of liquid egg white was used to produce 5-hydroxytryptophan (HTP) under various conditions and investigate the sleep-potentiating activity of liquid egg white hydrolysate (LEH) on pentobarbital-induced sleep. Alcalase hydrolysis yielded the highest content of 5-HTP ($13.50{\mu}g/mL$), while neutrase hydrolysis showed the lowest 5-HTP content ($5.23{\mu}g/mL$). The liquid egg white to water ratio (1:1) was optimal for the production of 5-HTP with high amino-nitrogen (A-N) content and degree of hydrolysis. The 5-HTP, amino-nitrogen, and degree of hydrolysis increased until 24 h of hydrolysis and slightly increased thereafter during hydrolysis with 2% and 5% enzyme addition. 5-HTP administration at doses of 6 and 9 mg/kg significantly increased sleep duration and decreased sleep latency time compared to that in the control (p<0.05). LEH (150 mg/mouse), which was equivalent to 5-HTP at 6 mg/kg, significantly decreased sleep latency time and increased sleep duration time compared to that in the control (p<0.05). Oral administration of LEH showed sleep-potentiating effects because of 5-HTP. The sleep-potentiating activity of LEH may have occurred through 5-HTP in our pentobarbital-induced sleep model. LEH may be a valuable alternative to sleep enhancement and may be used as a sleep-potentiating agent.

• 한국축산식품학회지
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• 제37권2호
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• pp.242-253
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• 2017

This study was performed to evaluate antioxidant activity of tomato powder extracted by various concentrations of ethanol (0, 25, 50, 75, 100%) and to evaluate the physicochemical properties and antioxidant activities of pork patties with ethanol extracted tomato (EET) powder. No differences in the contents of total of individual phenolic compounds including gallic acid and catechin, were observed among the treatments (p>0.05). Among the various concentrations, 50% and 75% EET powder showed the highest free radical scavenging and iron chelating activities (p<0.05). Lipid peroxidation was retarded in linoleic acid emulsion with the addition of 50% and 75% EET powder (0.1%). Based on the model study, five pork patties were actually manufactured; control patty, reference patty with 0.01% of butylated hydroxytoluene, patty with 1% of water extracted tomato (WET), and patties with 0.5 and 1.0% of EET. Addition of 1% WET and EET decreased pH value, and increased redness values of pork patties, as compared to the control (p<0.05). Pork patties with WET (1.0%) and EET (0.5% and 1.0%) had lower 2-thiobarbituric acid reactive substances values compared with control patties after 7 d of storage (p<0.05). Pork patties containing EET powder showed lower total bacterial and Enterobacteriaceae counts than control patties (p<0.05). In conclusion, WET and EET (50%) could be used as a natural antioxidant and antimicrobial agent in meat products.

• 접착 및 계면
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• 제17권4호
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• pp.149-154
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• 2016

일반적으로, 화학 물리적 변성이 수반되는 멸균방법을 적용하지 못하는 경우 필터여과에 의한 멸균 방법을 주로 사용한다. 하지만 바이러스의 경우 100 nm의 작은 크기로 인해 박테리아 제거에 사용되는 필터여과에 의한 멸균 방법을 적용하기가 어렵다. 본 논문에서는 화학 물리적 변성 없이 바이러스를 비활성화하는 물질 개발을 위하여, 카테콜기가 도입된 헤파린 고분자(Hep-C)를 합성하였다. Hep-C의 바이러스 비활성화 효과를 알아보기 위해서 표면이 노르에피네프린으로 코팅된 조직배양접시에 아데노연관바이러스(Adeno-associated virus; AAV)를 고정화하였으며, 그 위에 다시 Hep-C를 코팅하여 녹색형광단백질(GFP) 유전자를 전달할 수 있는 AAV의 비활성화 정도를 유세포분석기(FACS)를 이용하여 분석하였다. 그 결과 AAV에 Hep-C를 도포하면 99%에 달하는 비활성화 효과를 보였으며, 헤파린 고분자를 도포한 결과에 비해 강한 염 저항성을 확인할 수 있었다. 이러한 결과로 미루어 보아 제조된 Hep-C는 AAV 바이러스 제거의 효과적 방법으로 충분한 가능성을 가짐을 증명하였다.

• Journal of Microbiology and Biotechnology
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• 제30권3호
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• pp.325-332
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• 2020

Methyl linderone (ML), a cyclo-pentenedione, was isolated from the fruit of Lindera erythrocarpa Makino (family Lauraceae). This plant has well-known anti-inflammatory effects; however, the anti-cancer effects of ML have not yet been reported. Thus, in the present study we investigated the effects of ML on the metastasis of human breast cancer cells. We used 12-O-tetradecanoyl phorbol-13-acetate (TPA)-stimulated MCF-7 cells as the cell model to study the effects of ML on invasion and migration. ML was found to reduce the invasion and migration rate of TPA-stimulated MCF-7 cells. Moreover, it inhibited two metastasis-related factors, matrix metalloproteinase-9 (MMP-9) and interleukin-8 (IL-8), at the mRNA and protein expression levels, in TPA-treated MCF-7 cells. The mechanism by which ML exerted these effects was through the inhibition of translocation of activator protein-1 (AP-1) and signal transducer and activator of transcription-3 (STAT3), mediated via phosphorylation of extracellular signal-regulated kinase (ERK). Taken together, our findings indicated that ML attenuated the TPA-stimulated invasion and migration of MCF-7 cells by suppressing the phosphorylation of ERK and its downstream factors, AP-1 and STAT3. Therefore, ML is a potential agent for the treatment of breast cancer metastasis.

• 한국콘텐츠학회논문지
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• 제9권12호
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• pp.932-939
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• 2009

본 논문은 시맨틱 웹 서비스 환경에서 유아교육을 위한 각각의 서비스를 표현하는 메타 정보를 기술한 서비스 템플릿 객체 모델을 기반으로 그래픽한 서비스 템플릿 저작도구 시스템을 구현하였다. 제안한 시스템은 자동으로 웹 서비스를 선택하고 구성하여 적절한 서비스 플랜을 생성하여 로봇 서비스를 제공한다. 또한 STDL을 기반으로 URC용 서비스 템플릿 생성, 추가, 삭제, 수정 등의 기능뿐만 아니라 서비스 템플릿 리소스들에 대한 그래픽한 처리 기능을 제공한다. 서비스 템플릿 과정에서 사용자 편의적인 환경 제공을 위해 플로우 뷰 스타일, 그리드 뷰 스타일, 텍스트 뷰 스타일 등의 편집 환경을 구현했다. 또한 실제 로봇 구동에 필요한 로봇API를 참조하고 이를 기반으로 추상 서비스 블록을 구체화하여 서비스 템플릿을 쉽게 작성할 수 있는 기능을 제공하게 된다. 따라서 서비스의 시맨틱 정보를 기반으로 서비스 에이전트의 지능적이고 자율적인 서비스를 제공할 수 있다.

• 정보처리학회논문지B
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• 제17B권1호
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• pp.85-92
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• 2010

부분 의미 분석 시스템은 문장의 구성 요소들이 술어와 갖는 관계를 분석하는 것으로 문장에서 술어의 주체, 객체, 도구 등을 나타내는 의미 논항을 확인하게 된다. 본 논문에서 개발한 부분 의미 분석 시스템은 두 단계로 구성되어 있는데, 먼저 부분 구문 분석 결과로부터 의미 논항의 경계를 찾는 의미 논항 확인 단계를 수행하고 다음으로 확인된 의미 논항에 적절한 의미역을 부착하는 의미역 할당 단계를 수행한다. 순차적인 두 단계 방법을 적용하는 것에 의해서, 학습 성능 저하의 주요한 원인인 클래스 분포의 불균형 문제를 완화할 수 있고, 각 단계에 적합한 자질을 선별하여 사용할 수 있다. 본 논문에서는 PropBank 말뭉치에 기반한 CoNLL-2004 shared task의 데이터 집합 및 평가 프로그램을 사용하여 각 단계가 시스템의 전체 성능에 기여하는 정도를 보인다.