• Biomedical Science Letters
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• v.24 no.3
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• pp.168-174
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• 2018

Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by abdominal pain, rectal bleeding and diarrhea. Gastrodia elata Blume (GE) has been used for the treatment of various diseases including neurodegenerative diseases and inflammatory disease. However, there has been no information on whether GE regulates intestinal inflammation. The aim of this study is to elucidate whether GE can protect against dextran sulfate sodium (DSS)-induced colitis in a mouse model. The colitis mice were induced by drinking water containing 5% DSS for 7 days. Body weight, colon length and clinical score were assessed to determine the effects on colitis. The levels of inflammatory cytokines, tumor necrosis factor $(TNF)-{\alpha}$ and interleukin (IL)-6 in colitis tissue were also measured. The results showed that mice administrated with DSS showed clinical signs including weight loss and reduced colon length. GE inhibited the DSS-induced loss of body weight and shortening of colon and increased Disease activity index score. Additionally, we observed that GE suppressed the levels of $TNF-{\alpha}$ and IL-6 in DSS-treated colon tissues. Collectively, these findings provide experimental evidence that GE might be a useful therapeutic agent for patients with UC.

• Biomedical Science Letters
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• v.25 no.4
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• pp.398-406
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• 2019

Cancer immunotherapy using gene-modified tumor cells is safe and customized cancer treatment method. In this study, we made gene-modified tumor cells by transferring costimulatory molecules, 4-1BBL and OX40L, into tumor cells using lentivirus vector, and identified anti-cancer effect of gene-modified tumor cells in CT26 mouse colorectal tumor model. We construct pLVX-puro-4-1BBL, -OX40L vector for lentivirus production and optimized the transfection efficiency and transduction efficiency. The transfection efficiency is maximal at DNA:cationic polymer = 1:0.5 and DNA 2 ㎍ for lentivirus production. Then, the lentiviral including 4-1BBL and OX40L was used to deliver CT26 mouse tumor cells to establish optimal delivery conditions according to the amount of virus. The transduction efficiency is maximal at 500 μL volume of lentiviral stock without change in cell shape or growth rate. CT26-4-1BBL, CT26-OX40L significantly inhibited the tumor growth compare with CT26-WT or CT26-β-gal cell line. These data showed the possibility the use of genetically modified tumor cells with costimulatory molecule as cancer immunotherapy agent.

• YAKHAK HOEJI
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• v.51 no.6
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• pp.476-481
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• 2007

A polymorphic fungus, Candida albicans, causes various forms of infections such as disseminated candidiasis and vaginitis. Recent reports indicate that the fungus is a main etiological agent for the arthritis. In search of new sources for treatment of the fungal arthritis, we examined $18{\beta}$-glycyrrhetinic acid ($18{\beta}$-GA) against C. albicans-caused septic arthritis. The compound is isolated from Glycyrrhizae Radix that is known to have various immunomodulating activities and is one of the most popular herbal medicines. For induction of animal model of a septic arthritis, mice were given an emulsion form of C. albicans cell wall mixed with Complete Freund's Adjuvant (CFA) via footpad-injection. To determine prophylactic and therapeutic effects, the component was given to the animals before or after the induction of the arthritis, respectively. Data showed that intraperitoneal administration of $18{\beta}$-GA resulted in reduction of the inflammation, indicating the component had both prophylactic and therapeutic activities. For investigation of mechanism of the $18{\beta}$-GA, inhibitory effects on NO (nitiric oxide) and on T-lymphocyte proliferation were determined. Results demonstrated that $18{\beta}$-GA suppressed NO production from LPS (lipopolysaccharide)-treated macrophages and also inhibited proliferation of Con A (concanavalin A)activated T-cells. Taken together, $18{\beta}$-GA, a pentacyclic triterpene, has anti-arthritic activity against C. albicans-caused septic arthritis, possibly by blocking NO production and T-cell suppression.

• The Korean Journal of Zoology
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• v.36 no.4
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• pp.487-495
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• 1993

TRH (Thvrotropin-Releasing Hormone) known to regulate the transcription of the TSH (Thyroid-Stimulating Hormones gene in pituitary cells, but little is understood about the mechanism(sl involved. re present study was attempted to elucidate the role of Ca2+ movement through the voltage-gated channels in the regulation of TSH gene transcription. The c-fos is one of immediate early genes and used as model system for the investigation of signaling pathwavs involved in various stimuli. The changes of c-fos mRNA levels were determined after treatment of various agents using Northern and slot hybridization analysis. The c-fos mRNA was rapidly and transiently induced by TRH (about 3-fold) in GH3 cells and this induction was repressed by calcium chelating agent (EGTA), calcium channel blocker (verapamil) anti protein kinase C inhibitor (aminoacridine). The abilities of forskolin (adenvlate cvclase activators, PMA (protein kinase C activator), and A23187 (calcium ionophore) to affect c-ios gene transcription, either alone or in combination with TRH were tested in the same cells. All of them significantly increased the level of c-fos mRUA. However, no additive relationship was observed in all combined treatments except forskolin. These results suggest that TRH action on the c-fos gene activation is mediated by calcium influx as well as through protein kinase C.

• Journal of Institute of Control, Robotics and Systems
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• v.12 no.1
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• pp.66-71
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• 2006

Recently, there are lots of concerning on ubiquitous environment of robots and URC (Ubiquitous Robotic Companion). In this paper, a practical ubiquitous interface system far controlling mobile robots in indoor environments was proposed. The interface system was designed as a manager-agent model including a PC manager, a mobile manager, and robot agents for being able to be accessed by any network. In the system, the PC manager has a 3D virtual environment and shows real images for a human-friendly interface, and share the computation load of the robot such as path planning and managing geographical information. It also contains Hybrid Format Manager(HFM) working for transforming the image, position, and control data and interchanging them between the robots and the managers. Mobile manager working in the minimized computing condition of handsets has a mobile interface environment displaying the real images and the position of the robot and being able to control the robots by pressing keys. Experimental results showed the proposed system was able to control robots rising wired and wireless LAN and mobile Internet.

• International Journal of Internet, Broadcasting and Communication
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• v.7 no.1
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• pp.42-48
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• 2015

Generally, the current commercial cloud system is hosted by the centralization large datacenter. However, the other clouding forms existed and one of them is the P2P cloud. The P2P Cloud is a distributed systems, is freedom to join and leave and is possible to provide the IaaS. Commonly, P2P Cloud System is based on Pure-P2P System. Since a number of connection paths exist, it has a high reliability and easily scalability of the network. However, there are disadvantages of the slow rate of route because of looking up the all peers in order to search for the peer. In this paper, it proposes the P2P cloud system based on super-peer P2P. Super-peer P2P system has fast routing time, because of searching for cluster unit and it also can has high reliability and high scalability. We designs the super Peer cloud service and proposes the system model and Resource Allocation Algorithm for IaaS in Super peer P2P environment.

• Bulletin of the Korean Chemical Society
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• v.34 no.8
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• pp.2466-2472
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• 2013

Human immunodeficiency virus type-1 (HIV-1) is a causative agent of Acquired immunodeficiency syndrome (AIDS), which has affected a large population of the world. Viral envelope glycoprotein (gp120) is an intrinsic protein for HIV-1 to enter into human host cells. Molecular docking guided molecular dynamics (MD) simulation was performed to explore the interaction mechanism of heterobiaryl derivative with gp120. MD simulation result of inhibitor-gp120 complex demonstrated stability. Our MD simulation results are consistent with most of the previous mutational and modeling studies. Inhibitor has an interaction with the CD4 binding region. Van der Waals interaction between inhibitor and Val255, Thr257, Asn425, Met426 and Trp427 were important. This preliminary MD model could be useful in exploiting heterobiaryl-gp120 interaction in greater detail, and will likely to shed lights for further utilization in the development of more potent inhibitors.

• Clean Technology
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• v.14 no.3
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• pp.153-159
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• 2008

The solubility of triclosan, an anti-acne agent was measured in supercritical carbon dioxide ($scCO_2$) with a variable volume view cell at 313.15, 323.15, and 333.15 K and at pressures between 10 and 40 MPa. We successfully correlated triclosan solubility in $scCO_2$ using the quasi-chemical nonrandom lattice fluid (QLF) equation of state. Triclosan was micronized using the rapid expansion of supercritical solutions (RESS) process. The effects of temperature and pressure on particle size were investigated using phase behavior data and correlated results from the QLF model.

• The Journal of Society for e-Business Studies
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• v.9 no.4
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• pp.85-102
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• 2004

Currently, developed technologies for semantic web services discovery are based on ontologies. These ontologies are DAML-S(DARPA Agent Markup Language for Services) and Process Handbook Project of MIT. These technologies have some problems for intelligent web services discovery. So, in this paper we analyzed those ontologies and proposed TM-S, Topic Maps for Services. TM-S is the presentation model for semantic web services. And TM-S includes benefits and complements weaknesses of those ontologies. And we proposed TMS-QL, TM-S Query Language. TMS-QL is query language for intelligent web services discovery. At last, we designed and implemented intelligent web service discovery system that deals TM-S ontology and TMS-QL

• Journal of the Korea Society of Computer and Information
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• v.8 no.3
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• pp.34-39
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• 2003

There have been several researches to trace intruders on the Internet. But, up to now, few of them has shown a satisfactory and practicable result of the study. Recently, a little bit more active methods such as 'counter-attacking' have been considered to be an alternative to solve the problem of hacking, and some people showed a tendency to accept the method as one possible way to protect their systems. And the new intruder-retracing method suggested in this study is an improved AIAA(Autonomous Intrusion Analysis Agent) model which has been achieved by attaching the counter-attacking method to the existing tracing system. In this paper, the automatic intruder-tracking system is proposed, which was achieved through the design of the following three modules, such as the intruder-retracing module, intruder-tracing module and AIAA dispatch module.