• Environmental Engineering Research
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• v.10 no.5
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• pp.213-226
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• 2005

To treat wastewater efficiently by a one-step process of nitrogen removal, a new bacterial strain producing $N_2$ gas from ${NH_4}^+$ under an aerobic condition was isolated and identified. The cell was motile and a Gram-negative rod, and usually occurred in pairs. By 16S-rDNA analysis, the isolated strain was identified as Enterobacter asburiae with 96% similarity. The isolate showed that the capacity of $N_2$ production under an oxic condition was approximately three times higher than that under an anoxic condition. Thus, the consumption of ${NH_4}^+$ by the isolate was significantly different in the metabolism of $N_2$ production under the two different environmental conditions. The optimal conditions of the immobilized isolate for $N_2$ production were found to be pH 7.0, $30^{\circ}C$ and C/N ratio 5, respectively. Under all the optimum reaction conditions, $N_2$ production by the immobilized isolate resulted in reduction of ORP with both the consumption of DO and the drop of pH. The removal efficiencies of $COD_{Cr}$, and TN were 56.1 and 60.9%, respectively. The removal rates of $COD_{Cr}$, and TN were the highest for the first 2.5 hrs with the removal $COD_{Cr}/TN$ ratios of 32.1, and afterwards the rates decreased as reaction proceeded. For application of the immobilized isolate to a practical process of ammonium removal, a continuous operation was executed with a synthetic medium of a low C/N ratio. The continuous bioreactor system exhibited a satisfactory performance at 12.1 hrs of HRT, in which the effluent concentrations of ${NH_4}^+$-N was measured to be 15.4 mg/L with its removal efficiency of 56.0%. The maximum removal rate of ${NH_4}^+$-N reached 1.6 mg ${NH_4}^+$-N/L/hr at 12.1 hrs of HRT(with N loading rate of $0.08\;Kg-N/m^3$-carrier/d). As a result, the application of the immobilized isolate appears a viable alternative to the nitrification-denitrification processes.

• Journal of Korean Society on Water Environment
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• v.28 no.1
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• pp.63-70
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• 2012

This study was carried out to investigate change in influent concentration of domestic wastewater flowed from a newly constructed separate sewer system (SSS) and biological nutrients removal efficiency of a full scale Air-vent sequential batch reactor (SBR, $600m^3/d$). The average concentration of $BOD_5$, SS, T-N and T-P from SSS were 246.5 mg/L, 231.6 mg/L, 42.974 mg/L, 5.360 mg/L, respectively which corresponds to 2.2times, 1.2times, 1.8times and 2.1times higher than those from the conventional combined sewer system (CSS). The removal efficiency of $BOD_5$, SS, T-N, and T-P for the Air-vent SBR operated with influent from SSS averaged 99.1%, 99.0%, 91.2%, and 93.5%, respectively. Especially the respective nitrogen and phosphorus removal was 15% greater than that of the SBR operated with influent from CSS. Simultaneous nitrification and denitrification (SND) was observed in an aerobic reactor(II) as a result of DO concentration gradient developed along the depth by the Air-vent system. In order to achieve T-N removal greater than 90%, the C/N ratio should be over 6.0 and the difference between $BOD_5$ loading and nitrogen loading rate be over 100 kg/day (0.130 kg $T-N/m^3{\cdot}d$). Even with high influent T-P concentration of 5.360 mg/L from SSS (compared with 2.465 mg/L from CSS) T-P removal achieved 93.5% which was 15.5% higher than that of the SBR with influent from CSS. This is probably due to high influent $BOD_5$ concentration from SSS that could provide soluble carbon source to release phosphorus at anaerobic condition. In order to achieve T-P removal greater than 90%, the difference between $BOD_5$ loading and phosphorus loading rate should be over 100 kg /day (0.130 kg $T-N/m^3{\cdot}d$).

• Journal of Korean Society of Water and Wastewater
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• v.13 no.2
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• pp.55-65
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• 1999

AF(anaerobic filter)/BAF(biological aerated filter) system and UASB(upflow anaerobic sludge blanket)/BAF system, of which system effluents were recirculated to the anaerobic reactors in each system, were operated in order to investigate the performance in simultaneous removal of organics and nitrogen in high-strength dairy wastewater. Advanced anaerobic treatment processes of AF and UASB were evaluated on applicability as pre-denitrification reactors, and BAF was also evaluated on the performance in oxidizing the remaining organics and ammonia nitrogen. At system HRTs of 4.0 to 4.5 days and recirculation ratios of one to three, the AF/BAF system could achieve more than 99% of organics removals and 64 to 78% of total nitrogen removals depending upon the recirculation ratio. Although the UASB/BAF system also showed more than 99% of organics removals, total nitrogen removals in the UASB/BAF system were 53 to 66% which are lower than those in the AF/BAF system at the corresponding recirculation ratios. Optimum recirculation ratios considering simultaneous removal of organics and nitrogen and cost-effectiveness, were in the range of two to three. The upflow AF packed with crossflow module media, as a primary treatment of the anaerobic reactor/BAF system, showed better performances in denitrification, SS removals, and gas production than the UASB. Higher loading rate of suspended solids from the UASB increased the backwashing times in the following BAF. Especially, at a recirculation ratio of three in the UASB/BAF system, the increase in head loss due to clogging in the BAF caused frequent backwashing, at least once d day. The BAF showed the high nitrification efficiency of average 99.2% and organics removals more than 90% at organics loading rate less than $1.4KgCOD/m^3/d$ and $COD/NH_3-N$ ratio less than 6.4. It was proved that the simplified anaerobic reactor/BAF system could maximize the organics removal and achieve high nitrogen removal efficiencies through recirculation of system effluents to the anaerobic reactor. The AF/BAF system can, especially, be a cost effective and competitive alternative for the simultaneous removal of organics ana nitrogen from wastewaters.

• Journal of the Korean Society of Urban Environment
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• v.18 no.4
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• pp.401-408
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• 2018

EQPS (Effluent Quality Prediction System, Dynamita, France) was applied to analyze the appropriateness of the design of a bioreactor in A sewage treatment plant. A sewage treatment plant was designed by setting the design concentration of the secondary clarifier effluent to total nitrogen and total phosphorus, 10 mg/L and 1.8 mg/L, respectively, in order to comply with the target water quality at the level of the hydrophilic water. The retention time of the 4-stage BNR reactor was 9.6 hours, which was 0.5 for the pre-anoxic tank, 1.0 for the anaerobic tank, 2.9 for the anoxic tank, and 5.2 hours for the aerobic tank. As a result of the modeling of the winter season, the retention time of the anaerobic tank was increased by 0.2 hours in order to satisfy the target water quality of the hydrophilic water level. The default coefficients of the one step nitrification denitrification model proposed by the software manufacturer were used to exclude distortion of the modeling results. Since the process modeling generally presents optimal conditions, the retention time of the 4-stage BNR should be increased to 9.8 hours considering the bioreactor margin. The accurate use of process modeling in the design stage of the sewage treatment plant is a way to ensure the stability of the treatment performance and efficiency after construction of the sewage treatment plant.

• Journal of Korean Society on Water Environment
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• v.27 no.3
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• pp.322-328
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• 2011

This study was performed to investigate the characteristics of nutrient removal of municipal wastewater in the membrane bioreactor system with the different types of membrane. Membrane bioreactor consists of three reactors such as two intermittent anaerobic and the submerged membrane aerobic reactor with flat sheet and hollow fiber membrane, respectively. The removal efficiencies of $COD_{cr}$, BOD, SS, TN and TP on the flat sheet membrane bioreactor were 94.3%, 99.0%, 99.9%, 70.3% and 63.1%, respectively. In addition, The removal efficiencies of $COD_{cr}$, BOD, SS, TN and TP on the hollow fiber membrane bioreactor were 94.0%, 99.3%, 99.9%, 69.9% and 66.9%, respectively. The estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR) and phosphorus removal content on the flat sheet membrane bioreactor were $0.33kgVSS/kgBOD{\cdot}d$, $0.043mgNO_3-N/mgVSS{\cdot}d$, $0.031mgNH_4-N/mgVSS{\cdot}d$, and 0.144 kgP/d, respectively. In addition, the estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR) and phosphorus removal content on the hollow fiber membrane bioreactor were $0.30kgVSS/kgBOD{\cdot}d$, $0.067mgNO_3-N/mgVSS{\cdot}d$, $0.028mgNH_4-N/mgVSS{\cdot}d$, and 0.121 kgP/d, respectively. There was little difference between the flat sheet and hollow fiber on the nutrient removal efficiencies except SNR and SDNR. These differences between them were caused by the air demand to prevent the membrane fouling. The flux and oxygen demand for air scouring were $19.0L/m^2/hr$ and $2.28m^3/min$ for the flat sheet membrane, and $20.7L/m^2/hr$ and $1.77m^3/min$ for the hollow fiber membrane on an average.

• Journal of Korean Society of Water and Wastewater
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• v.31 no.1
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• pp.103-114
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• 2017

A membrane bioreactor by sequentially alternating the inflow and by applying a two-stage coagulation control based on pre-coagulation was evaluated in terms of phosphorus removal efficiency and cost-savings. The MBR consisted of two identical alternative reaction tanks, followed by aerobic, anoxic and membrane tanks, where the wastewater and the internal return sludge alternatively flowed into each alternative reaction tank at every 2 hours. In the batch-operated alternative reaction tank, the initial concentration of nitrate rapidly decreased from 2.3 to 0.4 mg/L for only 20 minutes after stopping the inflow, followed by substantial release of phosphorus up to 4 mg/L under anaerobic condition. Jar test showed that the minimum alum doses to reduce the initial $PO_4$-P below 0.2 mg/L were 2 and 9 mol-Al/mol-P in the wastewater and the activated sludge from the membrane tank, respectively. It implies that a pre-coagulation in influent is more cost-efficient for phosphorus removal than the coagulation in the bioreactor. On the result of NUR test, there were little difference in terms of denitrification rate and contents of readily biodegradable COD between raw wastewater and pre-coagulated wastewater. When adding alum into the aerobic tank, alum doses above 26 mg/L as $Al_2O_3$ caused inhibitory effects on ammonia oxidation. Using the two-stage coagulation control based on pre-coagulation, the P concentration in the MBR effluent was kept below 0.2 mg/L with the alum of 2.7 mg/L as $Al_2O_3$, which was much lower than 5.1~7.4 mg/L as $Al_2O_3$ required for typical wastewater treatment plants. During the long-term operation of MBR, there was no change of the TMP increase rate before and after alum addition.

• Journal of the Korean GEO-environmental Society
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• v.15 no.5
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• pp.39-46
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• 2014

The objectives of this study were to determine biodegradation and characteristics of BTEX and MTBE under aerovic-anaerobic conditions and evaluate the potential of natural attenuation method in denitrifying condition.. In the single-substrate experiments, all of the BTEX compounds were degraded under all the conditions. but, lower degradation of benzene and p-xylene were observed under aerobic condition due to the lack of oxygen initially supplied. In the mixed-substrate experiments, BTEX degradation was delayed compared to that in the single-substrate experiments due to a competition of the substrates. Biodegradation of MTBE was observed only under denitrifying conditions and we expected that MTBE mineralized to $CO_2$ without the accumulation of TBA. We also conducted to determine the effect of initial nitrate concentration on BTEX and MTBE degradation. At low nitrate concentration (<50 mg/L), BTEX degradations were limited by the lack of electron acceptor and BTEX degradation was inhibited at high nitrate concentration (>200 mg/L). The results in this study indicated that biotransformation could be applied to the gasoline-contaminated region under aerovic-anaerobic.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.343-346
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• 2000

This study was carried out to investigate the effects of influent $NH_4^{\;+}-N$ load, C/N ratio and superficial air velocity on the nitrogen removal efficiencies. Laboratory scale upflow biological aerated filter(BAF) was consisted of an anoxic-aerobic filter packed with porous ceramic media and operated with synthetic wastewater. BAFs requires less energy and space for the system when compared to conventional activated sludge process. The influent C/N ratios were varied from 0 to 1 by adjusting acetate. Various superficial air velocity had been applied to investigate aeration effect on nitrogen removal. The BAF reactor showed more than 90% average $NH_4^{\;+}-N$ removal efficiencies at $NH_4^{\;+}-N$ loading in the range of $0.26{\sim}1.33$ kg $NH_4^{\;+}-N/m^3{\cdot}d$ and 62% average T-N removal efficiencies at the C/N ratio of 1. Moreover, average T-N removal efficiencies increased as the superficial air velocity increased, because of the increase $NH_4^{\;+}-N$ removal efficiencies.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.10
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• pp.1757-1763
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• 2000

To consider the nutrient removal characteristics of BNR process activating soil microorganisms under the influence of DPB and to clear the characteristics of DPB under anoxic condition was investigated in the this study. The batch tests were conducted using sludge sampled from the BNR process activating soil microorganisms during operation periods. The results of this study were summarized as follows: - The DPB(Denitrifying Phosphorus removing Bacteria) performing denitrification and phosphorus uptake in the anoxic phase plays an important role in removing nitrogen and phosphorus in the BNR process activating soil microorganisms. - The PUR(Phosphorus Uptake Rate) of DPB in the anoxic phase was to be about 50% of PUR in the aerobic phase. - The DPB in the BNR process turned out to be increasing nutrient removal efficiency of BNR process.

• Journal of Korean Society on Water Environment
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• v.21 no.6
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• pp.624-629
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• 2005

The purpose of this study is to investigate the performance of nitrogen removal using autotrophic microorganism in the Membrane-Attached Biofilm Reactor (MABR). The treatment system consists of an aerobic MABR (R1) for nitrification and an anaerobic MABR (R2) for hydrogenotrophic denitrification. Oxygen and hydrogen were supplied through the lumen of hollow-fiber membranes as electron acceptor and donor, respectively. In phase Ι, simultaneous organic carbon removal and nitrification were carried out successfully in R1. In phase II, to develop the biofilm on the hollow-fiber membrane surface and to acclimate the microbial community to autotrophic condition, R1 and R2 were operated independently. The MABRs, R1 and R2 were connected in series continuously in phase III and operated at HRT of 8 hr or 4 hr with $NH_4{^+}-N$ concentration of influent, from 150 to 200 mgN/L. The total nitrogen removal efficiency reached the maximum value of 99% at the volumetric nitrogen loading rate of $1.20kgN/m^3{\cdot}d$ in the combined MABR system with R1 and R2. The results in this study demonstrated that the combined MABR system could operate effectively for the removal of nitrogen in wastewater not containing organic materials and can be used stably as a high rate nitrogen removal technology.